Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,344,2120AC40.8% 134.1 / 11.1 71S725A (TCA→GCA) arcBaerobic respiration two‑component sensor histidine kinase ArcB
Reads supporting (aligned to +/- strand):  ref base A (38/4);  new base C (0/29);  total (38/33)
Fisher's exact test for biased strand distribution p-value = 5.98e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCACCGTACTCTCCTCATCATCCTGGGTATCCCAG  >  NZ_CP009273/3343965‑3344459
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       
tGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGAcc                                                                                                                                                                                                                                                       >  1:419669/1‑250 (MQ=255)
 gTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGAccc                                                                                                                                                                                                                                                      >  1:452230/1‑250 (MQ=255)
  tAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGAcccc                                                                                                                                                                                                                                                     >  1:195827/1‑250 (MQ=255)
  tAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGAcccc                                                                                                                                                                                                                                                     <  1:358601/250‑1 (MQ=255)
    aCTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCccgc                                                                                                                                                                                                                                                   >  2:460797/1‑250 (MQ=255)
       catcatAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCcagccg                                                                                                                                                                                                                                                 >  2:28013/1‑249 (MQ=255)
             aatattTTGCGGCCCAGCCAAGTTTTCCCGGGTGTTGGCGCAGTTTCCGCGCCCCCCGTTCTAGCGGGGGTTTTTTTTTAGTGGCTTTTC‑CCCCCCCCGCTTTCACCATTTCTCCGCCGTGCCGCCCCTCTTCTTTCATCTCTCCAATCCATCCCCCGCCGTTATCTCCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGCCCCCGCCGCACCttt                                                                                                                                                                                                                                          <  2:442332/250‑1 (MQ=255)
                   ttACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTAGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAAtttt                                                                                                                                                                                                                                    >  2:8430/1‑250 (MQ=255)
                         cgcAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGtcc                                                                                                                                                                                                                              >  1:9416/1‑250 (MQ=255)
                            aGCCAAGATTTCCCTGTTTTGGGCGCATTATTCGCGCCCCCCGGTCTAGCCGGGGTTATTTTTTAGTGGCTTTTG‑CCCCCCACGCTTTCACCCTTTCTCCGTCGTGCCCCCCCTCTTCTTTCATCTCTTAAATCCATCCCCCGCCTTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGATTTTCCTCACCCAGTTTTTGCAGATGGCATACCCCCACGGCCCCCGCCGCACCTTTAATTTTATGtccttc                                                                                                                                                                                                                           <  2:617321/250‑1 (MQ=255)
                                 aGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTcaa                                                                                                                                                                                                                      >  2:675544/1‑250 (MQ=255)
                                          tGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCtt                                                                                                                                                                                                             >  2:569773/1‑250 (MQ=255)
                                            gtgtTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCtttt                                                                                                                                                                                                           >  1:602004/1‑250 (MQ=255)
                                             tgtTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGGGGCTTTGG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCCCTCTTCTTTCATCTCTCCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTGGCTGACCCAGCTGTTGCAGATGGCGTACCCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCttttt                                                                                                                                                                                                          <  1:60039/250‑1 (MQ=255)
                                                    gcAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTCTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTg                                                                                                                                                                                                   >  1:86231/1‑250 (MQ=255)
                                                      aGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTgcg                                                                                                                                                                                                 >  2:88033/1‑250 (MQ=255)
                                                          ttCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGt                                                                                                                                                                                             >  2:430666/1‑250 (MQ=255)
                                                           gcgccccccccGGTCTGGCGGGGTTCTTTTTTTAGTGGTTTTTG‑CCACCCCCGCTTCCACCGCTTCTCCGTCGGGACCCCTCTCTTTTTCCATCTCTTCAACCCTCCCCCCGCCTTTTTCTCCCCGGGCGGGAAGGCCAGGAGCCTAATTTTGCTGCCCCATTTTTTGCAGATGGCGAACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGt                                                                                                                                                                                             <  2:688018/242‑1 (MQ=255)
                                                                    ccGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGAc                                                                                                                                                                                   >  2:455423/1‑250 (MQ=255)
                                                                     cGGTCTAGCCGGGGTCTTTTTTTAGTGGCTTTTC‑CCCCCCACGCTTCCAGCACTTCTACGTCGTGACCCCACTCTTCTTCCATCTCTTCAATCCATTCACCGACGTTATCTCCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACt                                                                                                                                                                                  <  1:388686/250‑1 (MQ=255)
                                                                     cGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACt                                                                                                                                                                                  >  1:553081/1‑250 (MQ=255)
                                                                                 ggTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGa                                                                                                                                                                      >  2:169209/1‑250 (MQ=255)
                                                                                     tttttttTATTGCTTTTGCCCCCCCCCGCTTCCACCTCTCCTCGTTCGTGCCCCCCCTCTTCTTCCTCCTCTTCACTCCATCCCCCGCCTTTTTCTTCCCAGGCCGGAAGGCCGGGACACTGATTTTCCTCCCCCAGCTTTTGCAGATGGCGCACCCCCACCCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACa                                                                                                                                                                    <  2:8698/248‑1 (MQ=255)
                                                                                       tttttAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGc                                                                                                                                                                >  2:302278/1‑250 (MQ=255)
                                                                                                   tttG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCAtct                                                                                                                                                    >  1:4346/1‑250 (MQ=255)
                                                                                                        ccacccacGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCAtcttct                                                                                                                                                 >  2:355791/1‑249 (MQ=255)
                                                                                                           cccccGTTTACACCACTTCGCCGTCGTACCGCCACTTTCTTTTCACCCCTCCAACCAATACACCGACGTTATCTCCCCGGGCCGGAGGGCCAGGAGACTGATTTGCCTGCCCCAGCTGTTGCAGTTGCCGAACCCCCCCGCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCaaa                                                                                                                                             <  2:140253/250‑1 (MQ=255)
                                                                                                           cccacGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCaaa                                                                                                                                             >  2:518162/1‑250 (MQ=255)
                                                                                                            ccccGCTTTCACCACTTCTCCGTCGTGCCCCCCTTCTTCTTTCACCTCTCCAACCCATCCCCCGACTTTATCTCCCCAGGCCGGAAGGTCAGGAGACTAATTTTGCTGCCCCAGCTGTTGAAGATGGCTTACCCCCACGGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAac                                                                                                                                            <  1:353903/247‑1 (MQ=255)
                                                                                                             cacGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAaca                                                                                                                                           >  2:74699/1‑250 (MQ=255)
                                                                                                                 cTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGc                                                                                                                                       >  2:535037/1‑250 (MQ=255)
                                                                                                                 cTTTCACCCCTCTTACCTGGTGCCCCCACTCTTCTTTCCTCTCTACAATCCCTCCCCCCACGTTCTCTTCCCAGGCCGGAAGGTCAGGAGACTGATTTGGCTGACCCAGTTGTTGCAGATGGCAAACCCCCACGGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACAcc                                                                                                                                         <  2:636702/248‑1 (MQ=255)
                                                                                                                          cTTCTACGTCGTGACCCCACTCTTCTTTCATCTCTTCAACCCATCCCCCGACGTTATCTCCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGCCCCAGTTGTTGCAGAGGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTc                                                                                                                              <  1:460797/250‑1 (MQ=255)
                                                                                                                             cTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGt                                                                                                                           >  1:467952/1‑250 (MQ=255)
                                                                                                                                 gtctgacccccACTCTTTTTTCCTCTCTCAAACCCATTCCCCGCCTTTATCTCCCCAGGCCGGAAGGTCAGGAGACTGATTTTGCTCACCCTGTTGTTGCAGATGGCGCACCCCCACCGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATc                                                                                                                       <  2:401385/241‑1 (MQ=255)
                                                                                                                                 gtcgtGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATc                                                                                                                       >  2:431450/1‑250 (MQ=255)
                                                                                                                                   cgtGACCCCCCTCTTCTTTCATCTCTTCAATCCATCCCCCGACGTTATCTCCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGCCCCAGTTGTTGAAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAg                                                                                                                     <  1:184681/250‑1 (MQ=255)
                                                                                                                                             ctctTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTcc                                                                                                            >  1:125560/1‑249 (MQ=255)
                                                                                                                                                  cttCCACCTCTACAATCCATTCCCCGCCTTTATCTCCCCAGGCCGGAAGGTCAGAAGACTGATTTTGCTGCCCCAGTTGTTGCAGATGGCTTACCCCCCCTCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTAc                                                                                                         <  1:94530/247‑1 (MQ=255)
                                                                                                                                                          tctTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCgaga                                                                                               >  1:9432/1‑249 (MQ=255)
                                                                                                                                                              cAACCCATCCACCGCCGTTATCTCCCCAGGCGGAAAGGACGGGAGACTAAATTTCCTGCCCCAGTTGTTGCAGAGGGCGAACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTg                                                                                          <  2:257206/250‑1 (MQ=255)
                                                                                                                                                                 tCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTc                                                                                       >  2:179979/1‑250 (MQ=255)
                                                                                                                                                                   ctttCACCGACGTTATCCGCCCAGGCCGGAAGGCAGGGAGACTGAATTTGCTGACCCGGCTGTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCa                                                                                     <  1:8430/248‑1 (MQ=255)
                                                                                                                                                                    aTTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAg                                                                                    >  1:623464/1‑250 (MQ=255)
                                                                                                                                                                     tccACCGACTTTCTTTCCCCGGGCCGGAGGGCCGGGACACTGAGTTTGCGGACCCGGCTTTTGCAGATGGCGTACCCCCCCCGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGc                                                                                   <  2:404256/248‑1 (MQ=255)
                                                                                                                                                                       ccccGACGTTATCTCCCAGGGGGGGAAGGGGAGAAAATGGATTTTGCCGCCCCTGTTGTTGCAGAGGGCATACCCCCCCTCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCAt                                                                                 <  2:391330/248‑1 (MQ=255)
                                                                                                                                                                       acccGGCGTTACCTACCCGGCGCGGAGGGCCCCGATACGGAATTTCCTGCCCAGGCGGTGGCAGAGGGCCGACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCAt                                                                                 <  2:507056/248‑1 (MQ=255)
                                                                                                                                                                           gccGTTATCTCCCCAGGCGGGAAGGTCAGGAGACTGAATTTGCTGACCCAGTTGTTGCAGATGGCGTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGa                                                                             <  1:520598/248‑1 (MQ=255)
                                                                                                                                                                            aCGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGaa                                                                            >  2:66521/1‑250 (MQ=255)
                                                                                                                                                                                tATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATc                                                                        >  2:289313/1‑250 (MQ=255)
                                                                                                                                                                                  tCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATc                                                                        >  1:550069/1‑248 (MQ=255)
                                                                                                                                                                                   ctccccGGGCGGGAGGGCCGGGAGACTGATTTGCCTCCCCCAGCTGTTGAAGATGGCGCACCCCCCCCCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGa                                                                      <  2:655885/246‑1 (MQ=255)
                                                                                                                                                                                           gCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCtt                                                             >  2:315345/1‑250 (MQ=255)
                                                                                                                                                                                            ccGGAGGGTCAGGAGACTGAATTTGCTGCCCCAGCTGTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTc                                                            <  1:604437/250‑1 (MQ=255)
                                                                                                                                                                                            ccGGAAGGTCAGGAGACTGAATTGCCTGCCCCAGTTTTTGCAAAGGGCGAACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTc                                                            <  1:491053/250‑1 (MQ=255)
                                                                                                                                                                                              gcgagggcgggAGACAGAATTGCCTCCCCCATCTGTTGTAGAGGCAGCACCCCCACCCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTg                                                          <  2:499796/241‑1 (MQ=255)
                                                                                                                                                                                                aaGGTCAGGAGACTGAATTGGCTGCCCCAGCTGTTGCAGATGGCGAACCCCCACTCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGAt                                                        <  2:258356/250‑1 (MQ=255)
                                                                                                                                                                                                     cggcagattcaaTTTACTGACCCATCTGTTGCGTATGGCGTCCCCCCCCCCCCTCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACt                                                   <  2:586743/240‑1 (MQ=255)
                                                                                                                                                                                                             tGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCtctt                                           >  1:599991/1‑250 (MQ=255)
                                                                                                                                                                                                               aaTTCGCCGCCCCAGCTGTGGAAGGAGGCCTACCCCCACTCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCtcttct                                         <  2:493557/250‑1 (MQ=255)
                                                                                                                                                                                                                  tgccTGCCCCGGCTGTTGCAGAGGGCGTACCCCCACGACCCCCGCCGCACCTCTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTgt                                       <  2:86231/246‑1 (MQ=255)
                                                                                                                                                                                                                        aCCCATCTGTTGCAGATGCCGTACCCCCCCTCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCa                                  <  1:325475/248‑1 (MQ=255)
                                                                                                                                                                                                                           cAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCACCGTa                             >  1:71680/1‑250 (MQ=255)
                                                                                                                                                                                                                             gCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCACCGTAct                           >  2:179645/1‑250 (MQ=255)
                                                                                                                                                                                                                              cttttGCAGATGCCGTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCACCGTActc                          <  1:675544/247‑1 (MQ=255)
                                                                                                                                                                                                                                 ttGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTCCTGTTCTCTTCTGTCGTCACCGTACTctcc                       >  2:615683/1‑250 (MQ=255)
                                                                                                                                                                                                                                          gCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCACCGTACTCTCCTCATCATcc              <  1:394574/250‑1 (MQ=255)
                                                                                                                                                                                                                                                ccccccTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCACCGTACTCTCCTCATCATCCTGGGTa        <  1:315386/250‑1 (MQ=255)
                                                                                                                                                                                                                                                 cccACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCACCGTACTCTCCTCATCATCCTGGGTAt       <  1:88033/250‑1 (MQ=255)
                                                                                                                                                                                                                                                       gACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCACCGTACTCTCCTCATCATCCTGGGTATCCCAg  >  1:439466/1‑249 (MQ=255)
                                                                                                                                                                                                                                                       gACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCACCGTACTCTCCTCATCATCCTGGGTATCCCAg  >  2:543331/1‑249 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       
TGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTG‑CCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTACAAGTTCGAGATACTGTTCCAGCATGGGAATATCGAGCAATGCTTCTGATTTACTGTTCTCTTCTGTCGTCACCGTACTCTCCTCATCATCCTGGGTATCCCAG  >  NZ_CP009273/3343965‑3344459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑