Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,485,387	0	C	T	43.1%	64.4 / 28.3	53	Q90Q (CAG→CAA)	ppiA	peptidylprolyl isomerase A
Reads supporting (aligned to +/- strand):  ref base C (3/26);  new base T (22/0);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.47e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGG‑TTTTTTCTGCTGCATCTG‑CTC‑GGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCG‑A‑CAAAGTTTTGCA‑CAGACACTGGCGCTTTTTGTTTATCCA‑GCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATACGTGCGGGTCCCCTTTCGCTGCCATTGCTGCGGGAGAAAGAGCAGAAAGAGCGAAAACAGCAGCCAT  >  NZ_CP009273/3485150‑3485632                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                            gTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGT‑ACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGG‑TTTTTTCTGCTGCATCTG‑Ct                                                                                                                                                                                                                                               <  2:266339/250‑1 (MQ=255)       aGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGG‑TTTTTTCTGCTGCATCTG‑CTC‑GGt                                                                                                                                                                                                                                          <  1:462065/249‑1 (MQ=255)        gTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGG‑TTTTTTCTGCTGCATCTG‑CTC‑GGTGa                                                                                                                                                                                                                                        <  1:549395/250‑1 (MQ=255)            gCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGGTTGGC‑GGGGTTGG‑TTTTTTTTGCGGCCTCTG‑CTC‑gggtgaacc                                                                                                                                                                                                                                    >  2:142046/1‑243 (MQ=255)            gCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGG‑TTTTTTCTGCTGCATCTG‑CTC‑GGTGAAAcc                                                                                                                                                                                                                                    <  1:331229/250‑1 (MQ=255)                      aaTCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTCATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGG‑GTTTTTTTGCTGCATCTG‑CTC‑GGGGGAAACGCCGCCCTg                                                                                                                                                                                                                           >  1:616621/1‑249 (MQ=255)                        tCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGGTTTG‑TTTTTTTTTCTTCCTCTT‑CTC‑GGTGGAAACGCCGccccgcat                                                                                                                                                                                                                        >  1:515378/1‑245 (MQ=255)                         cTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTTG‑GTTTTTTTTCTGCCTCTT‑CTC‑GGGGGAAACGCCGCCCCGaaa                                                                                                                                                                                                                        >  1:487403/1‑248 (MQ=255)                           tATCGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTGTGG‑GGTTTTTTGCTTCCTCCG‑CTA‑CGTGGAAACGACGccccgtgtca                                                                                                                                                                                                                      >  2:191265/1‑242 (MQ=255)                                                tCACCACTTTACCAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGGTTGG‑TTTTTTTTTCTGCATCTT‑CTC‑GGTGGAAACGCCCCCCTGGATCATAAAACCAGGAATGACGCgggg                                                                                                                                                                                                >  1:447498/1‑248 (MQ=255)                                                tCACCACTTTACCAAATACCGCGTAACCGAAATCACACTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGGTTTTG‑TTTTTTTTTCGGC‑TCTG‑CTC‑GGGGGAACCGCCGCCCTGAATCATAAAAAAAGGGATGACGCgggg                                                                                                                                                                                                >  2:59524/1‑248 (MQ=255)                                                    cacTTTACCAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGGTTTGG‑TTTTTT‑TTCTGGCTCTT‑CTC‑GGGGGAAACGCCGCCCCGAATCATAAAACCAGGGATGAAGCGGGGGAAg                                                                                                                                                                                            >  1:78815/1‑250 (MQ=255)                                                           ccAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGG‑TTTTTTCTGCTGCATCTG‑CTC‑GGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAgtg                                                                                                                                                                                      <  1:291086/249‑1 (MQ=255)                                                           ccAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGGTTTG‑TTTTTTTTTCTTCCTCTG‑CTC‑GGTGGAACCCCCCCCCCCAAACCTAAAAACAGGAATGAAGCGGTGGGAGGTaaggg                                                                                                                                                                                     >  1:51493/1‑246 (MQ=255)                                                            cAAATACCGCGTACCCGAAATCACGCTGACCAGGGTCAAGGAAGTCGTGAGCGGCAACGTTGATAAAGAACGGGCTGGTGGCGGTGTCTTTTTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTGTTGATTGGC‑GAGTTTGG‑TATTTACAGCTGCATCTG‑CTC‑GGTGAAGCTGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGtgtt                                                                                                                                                                                    <  2:71777/250‑1 (MQ=255)                                                                tACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGG‑TTTTTTCTGCTGCATCTG‑CTC‑GGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTta                                                                                                                                                                                <  1:672036/250‑1 (MQ=255)                                                                     cgTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGGTTTG‑TTTTTTTTTCTGCTCCTGTCCC‑GGGTAAACGGCCCCCCGGAA‑CATAAAACCCGGCATGAGGCGGGGGAAGGGAGTGTTGTTTTaaa                                                                                                                                                                            >  2:471061/1‑249 (MQ=255)                                                                              aaTCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGG‑TTTTTTCTGCTGCATCTG‑CTC‑GGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGtt                                                                                                                                                                  <  1:330471/250‑1 (MQ=255)                                                                                tCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGGTTTG‑TTTTTTTTTCTTCTTCTT‑CTC‑GGTGGAAACGCCGCCCCTCATCATAAAAACAGGGAAGAAGCGGGGGAAGGGAGAGGTGGTTTTAAAAACGCTGGTca                                                                                                                                                                >  2:136456/1‑250 (MQ=255)                                                                                 cACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGGTTGGTTTTTTTTTGCTCCATCTT‑CCT‑GGGGAAACGCCCGCCCCGCACCTTAAACACCGAAATGATCCGGTGGAAGTGAGATGTGTTTTAAAAAcaccctgtg                                                                                                                                                                 >  2:166716/1‑241 (MQ=255)                                                                                    gCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGGTTTG‑TTTTTTTTGCTGCCTCTT‑CTC‑GGGGGAAACGCCCCCCCGGATCATAAAACCAGGGAAGAAGCGGGGGAAGGGAGGGGTGTTTTAAAAAACCCTGTTTACAt                                                                                                                                                             >  1:420331/1‑249 (MQ=255)                                                                                        aCCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGG‑TTTTTTCTGCTGCATCTG‑CTC‑GGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAAt                                                                                                                                                          <  2:392978/248‑1 (MQ=255)                                                                                          cATGGTCAAGGAGGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGG‑TTTTTTCTGCTGCATCTG‑CTC‑GGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCG‑A‑c                                                                                                                                                    <  2:353774/250‑1 (MQ=255)                                                                                                     aaggCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGGTTTG‑TTTTTTTTGCTGCCTCTG‑CTC‑GGGGGAAACGCCGCCCCGGATCATAAAACCAGGGATGACGCGGGGGAAGGGAGTGGTTTTTTAAAAAACCCCGTTTACATAATCGAA‑CAAAGttttca                                                                                                                                          >  1:234269/1‑248 (MQ=255)                                                                                                        gCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGG‑TTTTTTCTGCTGCATCTG‑CTC‑GGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCG‑A‑CAAAGTTTTGCA‑CAg                                                                                                                                     <  2:599645/250‑1 (MQ=255)                                                                                                        gCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGGTTGG‑TTTTTTTTTTTTCCTCTC‑CTC‑GGGGGAAACGCCCCCCTGTATCTTAAAAACCGGAACGACACGGGGTAAGGTGGGGTTGTTTTTAAAACACCTGTTCTCCTAATCT‑C‑CAAAAtttttctca                                                                                                                                       >  2:375559/1‑244 (MQ=255)                                                                                                           ttATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGG‑TTTTTTCTGCTGCATCTG‑CTC‑GGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCG‑A‑CAAAGTTTTGCA‑CAGaca                                                                                                                                  <  2:637850/250‑1 (MQ=255)                                                                                                             aTCGGCAACGGTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGG‑TTTTTTCTGCTGCATCTG‑CTC‑GGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCG‑A‑CAAAGTTTTGCA‑CAGacac                                                                                                                                 <  2:272302/249‑1 (MQ=255)                                                                                                              gcGGCAACGTTGATAAAGAACTGGCTGGAGGCGCTTTCTTTGTCAGGGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATATTTGATTGGC‑GGGTTTGG‑TTTTTTCTGCTGCATCGG‑CTC‑GGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTAAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCG‑A‑CAAAGTTTTGCA‑CAGACACt                                                                                                                                <  2:357428/248‑1 (MQ=255)                                                                                                                   aaCGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGGTTGG‑GTTTTTTTTCTGCATCTG‑CTC‑GGGGGAAACGCCGCCCCGAATCATAAAAACAGGGATGACGCGGGGGAAAGGAGGGGTTTTTTAAAAAACCCTGTTTACATAATCG‑A‑AAAAAATTTTCA‑CAAACAATGgcgcc                                                                                                                          >  1:124046/1‑249 (MQ=255)                                                                                                                          aTAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTTTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGG‑TTTTTTCTGCTGCATCTG‑CTC‑GGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCG‑A‑CAAAGTTTTGCA‑CAGACACTGGCGCTTTTTGt                                                                                                                    <  2:475983/249‑1 (MQ=255)                                                                                                                                  ctggctggTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGGTTTGG‑TTTTTT‑TTCTGCATCTG‑CTC‑GGGGGAAACGCCCCCCCGAATCATTAAAACAGGAAAGGAGGGGGGGAAGGGAGGGGTGGTTTTAAAAAAACCGTTCACATAAACG‑A‑CAAAGGTTTTTA‑CAAACACCGGCGCTttttttttttttatc                                                                                                           >  2:61376/1‑243 (MQ=255)                                                                                                                                         gtggCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGG‑TTTTTTCTGCTGCATCTG‑CTC‑GGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCG‑A‑CAAAGTTTTGCA‑CAGACACTGGCGCTTTTTGTTTATCCA‑GCTCCAGtt                                                                                                   <  2:200705/250‑1 (MQ=255)                                                                                                                                         gtggCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGG‑TTTTTTCTGCTGCATCTG‑CTC‑GGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCG‑A‑CAAAGTTTTGCA‑CAGACACTGGCGCTTTTTGTTTATCCA‑GCTCCAGt                                                                                                    <  2:224569/249‑1 (MQ=255)                                                                                                                                                                 cGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGG‑TTTTTTTTTCTGCCTCTG‑CTC‑GGTGGAAACGCCGCCCTGAATCATAAAACCAGGGATGACGCGGGGGAAGGGAGTGTTGTTATAAAAAACGCTGTTCACATAATCC‑A‑CAAAGATTTTCA‑CAGACACCGGCGCCTTTTGTTTTTTCA‑GCTCCCGTTCGATGTTACCAGCTGAGGTTGTc                                                                            >  1:606765/1‑249 (MQ=255)                                                                                                                                                                   tGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGGTTGG‑TTTTTTTTGCTGCATCTG‑CTC‑GGGGGAAACGCCGCCCTGAATCATAAAAACAGGAATGACGCGGGGGAAAGGAGGGTTGTTATAAAAAACCCTGTTTACCAAAACG‑A‑CAAAATTTTGCA‑CAAACACTGGGGCTTTTTTTTTATCCA‑GCTCCAATTCGATGTTACCAGATGAGGGTGTcaa                                                                          >  1:144260/1‑249 (MQ=255)                                                                                                                                                                      cATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGG‑TTTTTTCTGCTGCATCTG‑CTC‑GGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCG‑A‑CAAAGTTTTGCA‑CAGACACTGGCGCTTTTTGTTTATCCA‑GCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAAt                                                                      <  1:429801/250‑1 (MQ=255)                                                                                                                                                                                     aCGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGG‑TTTTTTCTGCTGCATCTG‑CTC‑GGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCG‑A‑CAAAGTTTTGCA‑CAGACACTGGCGCTTTTTGTTTATCCA‑GCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATACGTGCGGGTCCCCt                                                       <  1:46082/250‑1 (MQ=255)                                                                                                                                                                                       gcgTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGG‑TTTTTTCTGCTGCATCTG‑CTC‑GGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCG‑A‑CAAAGTTTTGCA‑CAGACACTGGCGCTTTTTGTTTATCCA‑GCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATACGTGCGGGTCCCCttt                                                     <  1:441040/250‑1 (MQ=255)                                                                                                                                                                                             tGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTTG‑GTTTTTTTTCTGCATTTG‑GTCGGGGGAAACCG‑CGCCCCTAATCATAAAAACCGGGAAGACGCGGGGGAAAGGAGGGGTGTTTTTAAAAAACCCGCTTATATAAACG‑A‑CAAAGGTTTTCACCAACCACT‑GCGCCTTTTTTTTATTCATACCCCACTT‑GATGTTATGAAACGAGGGTGTCAACAAAAACTGGGGGGCCCCTTTTTCTGc                                               >  2:343438/1‑250 (MQ=255)                                                                                                                                                                                                   ggCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGG‑TTTTTTTTGCTGCATCTT‑CTC‑GGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGGAGTGTTGTTATAAAAACCGCTGTTCACATAATCG‑A‑CAAAGTTTTGCA‑CCGACACTGGCGCTTTTTTTTTTTTCA‑GCTCCCGTTCGATGTTACCAGCTGAGGGTGTCAACAATACGTGCGGGGCCCCTTTCGCTGCCATtgg                                         >  1:437167/1‑249 (MQ=255)                                                                                                                                                                                                    gCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGG‑TTTTTTTTCGTGCATATG‑CTC‑GGTTTAAACGCCGCCCCGTATCATAAAAACCGGAGTGACGCGGTGAAAAGGAGAGTTGTTATAAAAACAGCCGTTTACACAATCG‑ACCAAAGTTTT‑CA‑CAAAAACATGCGGTTTTTTTTTTTTCA‑ACTCCCGTTTTGTGTTACCATCTGAGGGGGTCTACAATACAGGCGGGTGCCCCTTTTCTGCCCTttct                                        >  2:124980/1‑250 (MQ=255)                                                                                                                                                                                                       gTTATCGGCTTCATTTTTGATTGGC‑GGGTTTTG‑TTTTTTTTTCTGCATCTG‑CTC‑GGTGGAAACGCCGCCCTGAATCATAAAACCAGGGATGACGCGGGGGAAGGGAGTGTTGTTATAAAAAACGCCGGTCACATAAACG‑A‑CAAAAATTTTCA‑CAAACACCGGCGCTTTTTTTTTATTCA‑CCTCCCGTTTGATGTTACCCACTGAGGGTGTCAACAAAACATGCGGGGCCCCTTTCGCCTCCCTTGCTGCg                                     >  2:42813/1‑250 (MQ=255)                                                                                                                                                                                                       gTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGG‑TTTTTTCTGCTGCATCTG‑CTC‑GGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCG‑A‑CAAAGTTTTGCA‑CAGACACTGGCGCTTTTTGTTTATCCA‑GCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATACGTGCGGGTCCCCTTTCGCTGCCATTGCTGCg                                     <  1:484715/250‑1 (MQ=255)                                                                                                                                                                                                        ttATCGGCTTCATTTTTGATTGGC‑GGGTTTGG‑TTTTTTCTGCTGCATCTG‑CTC‑GGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCG‑A‑CAAAGTTTTGCA‑CAGACACTGGCGCTTTTTGTTTATCCA‑GCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATACGTGCGGGTCCCCTTTCGCTGCCATTGCTGCgg                                    <  2:512032/250‑1 (MQ=255)                                                                                                                                                                                                         tATCGGCTTCATTTTTGATTGGC‑GGGTTTGG‑GTTTTTTTGCTGCATCTG‑CTC‑GGGGAAAACGCCGCCCTGGATCATAAAACCAGGAATGACGCGGGGGAAGGTAGTGGTGTTTTAAAAAACGCTGTTCACATAAACG‑A‑CAAAGTTTTTCA‑CAAAAACTGGCGCTTTTTGTTTTTCCA‑GCTCCAGTTCGATGTTTCCAGCTGAGGTTGTCAACAAAACGTGCGGGGCCCCTTTCGCTGCCCTTGCTGCggg                                   >  1:64163/1‑250 (MQ=255)                                                                                                                                                                                                               cTTCATTTTTGATTGGC‑GGGTTTGG‑TTTTTTCTGCTGCATCTG‑CTC‑GGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCG‑A‑CAAAGTTTTGCA‑CAGACACTGGCGCTTTTTGTTTATCCA‑GCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATACGTGCGGGTCCCCTTTCGCTGCCATTGCTGCGGGagaaag                             <  1:34453/250‑1 (MQ=255)                                                                                                                                                                                                                          aTTGGC‑GGGTTTGG‑TTTTTTCTGCTGCATCTG‑CTC‑GGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCG‑A‑CAAAGTTTTGCA‑CAGACACTGGCGCTTTTTGTTTATCCA‑GCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATACGTGCGGGTCCCCTTTCGCTGCCATTGCTGCGGGagaaagagcagaaagag                  <  1:461870/250‑1 (MQ=255)                                                                                                                                                                                                                              gC‑GGGTTTGG‑TTTTTTCTGCTGCATCTG‑CTC‑GGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCG‑A‑CAAAGTTTTGCA‑CAGACACTGGCGCTTTTTGTTTATCCA‑GCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATACGTGCGGGTCCCCTTTCGCTGCCATTGCTGCGGGAGAAAGAGCCGAAAGAGCGa               >  2:449349/1‑249 (MQ=255)                                                                                                                                                                                                                              gC‑GGGTTTGG‑TTTTTTCTGCTGCATCTG‑CTC‑GGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCG‑A‑CAAAGTTTTGCA‑CAGACACTGGCGCTTTTTGTTTATCCA‑GCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATACGTGCGGGTCCCCTTTCGCTGCCATTGCTGCGGGAGAAAGAGCAGAAAGAGCGa               >  1:4238/1‑249 (MQ=255)                                                                                                                                                                                                                                 gggTTTGG‑TTTTTTCTGCTGCATCTG‑CTC‑GGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCG‑A‑CAAAGTTTTGCA‑CAGACACTGGCGCTTTTTGTTTATCCA‑GCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATACGTGCGGGTCCCCTTTCGCTGCCATTGCTGCGGGAGAAAGAGCAGAAAGAGCGaaaa            >  1:322049/1‑250 (MQ=255)                                                                                                                                                                                                                                        g‑tttTTTCTGCTGCATCTG‑CTC‑GGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCG‑A‑CAAAGTTTTGCA‑CAGACACTGGCGCTTTTTGTTTATCCA‑GCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATACGTGCGGGTCCCCTTTCGCTGCCATTGCTGCGGGAGAAAGAGCAGAAAGAGCGAAAACagcagc     <  1:486874/250‑1 (MQ=255)                                                                                                                                                                                                                                             tttCTGCTGCATCTG‑CTC‑GGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCG‑A‑CAAAGTTTTGCA‑CAGACACTGGCGCTTTTTGTTTATCCA‑GCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATACGTGCGGGTCCCCTTTCGCTGCCATTGCTGCGGGAGAAAGAGCAGAAAGAGCGAAAACAGCAGCCAt  <  1:149060/249‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                            GTCATGAGTCGGCACCTGGGAAATCTTATCGGCAACGTCCATGCCTTTCACCACTTTACCAAATACCGCGTAACCGAAATCACGCTGACCATGGTCAAGGAAGGCGTTATCGGCAACGTTGATAAAGAACTGGCTGGTGGCGCTGTCTTTGTCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGG‑TTTTTTCTGCTGCATCTG‑CTC‑GGTGAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCG‑A‑CAAAGTTTTGCA‑CAGACACTGGCGCTTTTTGTTTATCCA‑GCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATACGTGCGGGTCCCCTTTCGCTGCCATTGCTGCGGGAGAAAGAGCAGAAAGAGCGAAAACAGCAGCCAT  >  NZ_CP009273/3485150‑3485632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑