Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,544,9360AG39.2% 69.8 / 18.7 51I182V (ATC→GTC) uxuRUxu operon transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (4/27);  new base G (20/0);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.37e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCGCGGCAGTTATTGGAGAGCAACATCGCCGAGTTTGCCGCTTTGCAGGCTACCCGCGAAGATATCGTCAAAATGCGTCAGGCATTGCAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGC‑GTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTC‑TGCTGGAA‑TTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGC  >  NZ_CP009273/4544693‑4545183
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                            
gcgcGGCAGTTATTGGAGAGCAACATCGCCGAGTTTGCCGCTTTGCAGGCTACCCGCGAAGATATCGTCAAAATGCGTCAGGCATTGCAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGt                                                                                                                                                                                                                                                       <  2:136305/250‑1 (MQ=255)
       aGTTATTGGAGAGCAACATCGCCGAGTTTGCCGCTTTGCAGGCTACCCGCGAAGATATCGTCAAAATGCGTCAGGCATTGCAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCCAAGTGGGT‑CCAGTTTCACAg                                                                                                                                                                                                                                                >  1:256153/1‑250 (MQ=255)
            ttGGAGAGCAACATCGCCGAGTTTGCCGCTTTGCAGGCTACCCGCGAAGATATCGTCAAAATGCGTCAGGCATTGCAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATc                                                                                                                                                                                                                                           <  1:478852/250‑1 (MQ=255)
                 gagCAACATCGCCGAGTTTGCCGCTTTGCAGGCTACCCGCGAAGATATCGTCAAAATGCGTCAGGCATTGCAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGGAACAAACCAAAGTGGGTGCCGGTTGCAC‑GGCATCTgggg                                                                                                                                                                                                                                      >  2:416177/1‑248 (MQ=255)
                        aTCGCCGAGTTTGCCGCTTTGCAGGCTACCCGCGAAGATATCGTCAAAATGCGTCAGGCATTGCAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAAAAAAACAATGTGGGT‑CCCGTTGCCCCGCCACCTGGGTGACAgac                                                                                                                                                                                                                               >  2:128466/1‑248 (MQ=255)
                               aGTTTGCCGCTTTGCAGGCTACCCGCGAAGATATCGTCAAAATGCGTCAGGCATTGCAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAAACAATGTGGGG‑CCAGTTGCACAACCCTCTGGGAGACAGCCTGTATCg                                                                                                                                                                                                                        >  1:600566/1‑250 (MQ=255)
                                       gCTTTGCAGGCTACCCGCGAAGATATCGTCAAAATGCGTCAGGCATTGCAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAACAATCCAATTTTGGT‑CCCGTTGCCCAGCCCTCTGGGTGACAACCTTTTTTGCCAAGA‑Gt                                                                                                                                                                                                               >  1:257835/1‑250 (MQ=255)
                                             cAGGCTACCCGCGAAGATATCGTCAAAATGCGTCAGGCATTGCAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTgg                                                                                                                                                                                                         <  2:645965/250‑1 (MQ=255)
                                               ggCTACCCGCGAAGATATCGTCAAAATGCGTCAGGCATTGCAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAACAAACCAAAGTGGGT‑TCCGTTGCTCAGACATATGGGGGGCAACCTCGATCGCACaggggtgggtggt                                                                                                                                                                                                        >  2:521289/1‑239 (MQ=255)
                                                   aCCCGCGAAGATATCGTCAAAATGCGTCAGGCATTGCAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAATACAATGTGGGT‑CCCGTTGCACAACCCTCTTGGTGACAGCCTGTATCGCACAGAGGGGGTTgggggg                                                                                                                                                                                                     >  2:220040/1‑246 (MQ=255)
                                                      cgcgAAGATATCGTCAAAATGCGTCAGGCATTGCAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATcaca                                                                                                                                                                                                <  1:16722/250‑1 (MQ=255)
                                                       gcgAAGATATCGTCAAAATGCGTCAGGCATTGCAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCCATGTGGGT‑CCAGTTGCCCCGCCCTCTTGGTGACATACCGTATTGCAAAGA‑GGGGGTTGGGGGTcaca                                                                                                                                                                                                >  2:468264/1‑249 (MQ=255)
                                                             atatCGTCAAAATGCGTCAGGCATTGCAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAACAATCCAATGTGGGT‑CCAGGTGCACAGCCATCTGGGTGACAGCCTGTATTGCAAAGA‑GGGGGTGGGCGATCACaaa                                                                                                                                                                                              >  1:8467/1‑245 (MQ=255)
                                                             atatCGTCAAAATGCGTCAGGCATTGCAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATCACAAACAGAt                                                                                                                                                                                         <  2:608544/250‑1 (MQ=255)
                                                                        aTGCGTCAGGCATTGCAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGC‑Gt                                                                                                                                                                             <  2:614271/250‑1 (MQ=255)
                                                                                      gCAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGC‑GTTAATCAAAAAAGa                                                                                                                                                                <  2:531355/249‑1 (MQ=255)
                                                                                                gagCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAAAAAACCAATGTGGGG‑CCAGTTGCACAGCCCTCTGGGTGACAGACCGTATCGCCAAAA‑GGGGGTTGGGGGGCACAAAAAAAACCTCCCCCCGGTTAACAAAAAAAAATGCCC‑Agcgg                                                                                                                                                     >  2:213751/1‑250 (MQ=255)
                                                                                                  gCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAATGTGGGT‑CCAGGTGCACAACCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGGTGGGCGGTCACAAAAAGATCCTCCCCGC‑CGTAATCAAAAAAGATGCCCGAgcggcg                                                                                                                                                   >  1:34784/1‑250 (MQ=255)
                                                                                                       aaCTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGC‑GTTAATCAAAAAAGATGCCCGAGCGGCGAAGCt                                                                                                                                              <  2:691170/250‑1 (MQ=255)
                                                                                                       aaCTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGC‑GTTAATCAAAAAAGATGCCCGAGCGGCGAAGCt                                                                                                                                              <  2:279862/250‑1 (MQ=255)
                                                                                                           ggCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAAAAATCCCATGTGGGT‑CCAGTTGCACAGCCCTCTGGACGAAAGCCCGTATCGCCAAGA‑GGGGGTTGGGGGTCTCCAACAGATCCTCCCCCC‑CTTAAACAAAAAAAAAGGCCGAGCGGGGGAGCgggg                                                                                                                                           >  2:512292/1‑248 (MQ=255)
                                                                                                           ggCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAAAAAAACAAAGTGGGA‑CCAGTTGTACAGCCCTCTGGATGACAGCCCGTATTGCCAAAA‑GGGGGTGGGGGGTTACCAAAAGAATCTCCCCCG‑GTTAATCAAAAAAAAAGACCCAGCGGCGCAGATGGcc                                                                                                                                          >  2:225662/1‑249 (MQ=255)
                                                                                                                 cAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGC‑GTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTgg                                                                                                                                    <  1:371590/250‑1 (MQ=255)
                                                                                                                       gcCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGC‑GTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCAt                                                                                                                              <  1:379119/250‑1 (MQ=255)
                                                                                                                                         cGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGcac                                                                                                                                                                                                                                                  <  1:289614/118‑1 (MQ=255)
                                                                                                                                                       tCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGGT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GGGGTTGGGCGATCACAAACAGATCCTCGCCGC‑GTTAATCAAAAAAGAAGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCAACTGGGAAAAGTTAAGCAACGGC‑TGCTGGGA‑tt                                                                                            >  1:559624/1‑250 (MQ=255)
                                                                                                                                                        ccATCTCGCGATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGC‑GTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTC‑TGCTGGAA‑tt                                                                                            <  1:398583/249‑1 (MQ=255)
                                                                                                                                                               gCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGC‑GTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTC‑TGCTGGAA‑TTCTCGAAc                                                                                     <  1:484022/249‑1 (MQ=255)
                                                                                                                                                               gCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGC‑GTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTC‑TGCTGGAA‑TTCTCGAAc                                                                                     <  1:342242/249‑1 (MQ=255)
                                                                                                                                                                          aGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAAACCAATGTGGGT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GGGGGTGGGGGGTCACAAAAAAATCCTCGCCGC‑GTTTATCAAAAAAAATGGCCGAGCGGCGAAGGTGGGAATGTGGGAGCCTCTGGAAAAAGTTAAGAAAACTCTTGTTGGAA‑TTTCTGAAGGTTG‑CGAtatt                                                                         >  2:672374/1‑250 (MQ=255)
                                                                                                                                                                             aaCGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGGTGACAGCCCGTATCGCAAAGA‑GTGGGTGGGCGGTCACAAAAAAATCCTCGCCGC‑GTTAATCAAAAAAGAAGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGAC‑TGCTGGGA‑TTCTTGTACGGTGACGAtattttt                                                                      >  1:267149/1‑248 (MQ=255)
                                                                                                                                                                             aaCGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAAAGTGGGT‑CCAGGTGCACAGCCATCTGGGTGACAGCCTGTATCGCAAAGA‑GGGGTTGGGGGGTCACAAACAGATCCCTGCCCC‑GTTTATCAAAAAAAATGCCCGGGCGGCGGAACCGGGCATGGGGGAGCAACTGGAAAACGGTCAACACCGTC‑TGGTGGAATTTTTGGAAGGTTGCGGAtatttt                                                                       >  2:172915/1‑249 (MQ=255)
                                                                                                                                                                                 cATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAAACCCATGTGGGT‑CCAGTTGCACAGCCATCTGGGTGACAGCCTGTATCGCAAAGA‑GGGGGTGGGCGCTCCCACACAAAACCCCGCCCC‑CTTTATCAAAAAAGAAGGCCGCGCGGGCGAGCTGGCCATGTGGGCGCATCTGGGAAAAGTTTAGAAACGCC‑TGCTGGGA‑TTCTCGAACGTTGACGAGCTTTTTTTTg                                                                  >  2:37298/1‑250 (MQ=255)
                                                                                                                                                                                   tAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGGT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGGTGGGGGATCACAAACAAAACCTCGCCGC‑GTTTATCAAAAAAGATGCCCGAGCGGCGGAGCTGGGAATGTGGCAGCAACTGGGAAAAGTTAAGCAACGTC‑TGCTGGAA‑TTCTTGAACGGTGACGATATTTTTTTTGAt                                                                >  1:564558/1‑250 (MQ=255)
                                                                                                                                                                                   tAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGGTGACAGCCTGTATTGCCAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGC‑GTTAATCAAAAAAGATGCCCGAGCGGCGAAACTGGCAATGTGGCAGCATCTGGAAAACGGTAAGCAACGTC‑TGCTGGAA‑TTTTCCAACGTTGACGATATTTTTTTTTAt                                                                >  1:674522/1‑250 (MQ=255)
                                                                                                                                                                                    aaCAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAATACAATGTGGGT‑CCAGTTGCACAGCCATCTGGGTGAAAGCCTGTATCGCAAAGA‑GGGGGTGGGGGGTCACAAAAAGATCCTCGCCGC‑GTTAATAAAAAAAAATGCCCGAGCGGCGGAGCTTGGAAAGGGGGAGGATCTGGAAAAAGTTAAAGAACGTC‑CGCTGGAA‑TTCTCGAACGGTGGAGGTATTTAtttttttg                                                               >  2:111191/1‑246 (MQ=255)
                                                                                                                                                                                           tGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGC‑GTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTC‑TGCTGGAA‑TTCTCGAACGTTGACGATATTTATTTTGATGGCTATCt                                                        <  1:18910/250‑1 (MQ=255)
                                                                                                                                                                                                 tggAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGC‑GTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTC‑TGCTGGAA‑TTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGa                                                  <  2:208358/250‑1 (MQ=255)
                                                                                                                                                                                                 tggAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGC‑GTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTC‑TGCTGGAA‑TTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGa                                                  <  1:470543/250‑1 (MQ=255)
                                                                                                                                                                                                         ttCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGC‑GTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTC‑TGCTGGAA‑TTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGc                                          <  2:417053/250‑1 (MQ=255)
                                                                                                                                                                                                         ttCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGC‑GTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTC‑TGCTGGAA‑TTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGc                                          <  1:340817/250‑1 (MQ=255)
                                                                                                                                                                                                           ccGTCAGTCCTGGCAGTGGCGGGAAAACAAACCAATGTGGGT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GGGGGTGGGCGGTCACAAAAAAAACCTCGCCGC‑GGTAATCAAAAAAAAAGCCCGAGCGGCGAAGCTGGGAATGTGGCAGCATCTGGGAAAAGGTAAGCAAAGTC‑TGCTGGGA‑TTCTCGAACGGTGACGATATTTATTTTGTTGGGTAACTGGTTGATTCATGGCCg                                        >  1:96354/1‑250 (MQ=255)
                                                                                                                                                                                                                 gTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGC‑GTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTC‑TGCTGGAA‑TTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGAt                                  <  2:34784/250‑1 (MQ=255)
                                                                                                                                                                                                                    cTGGCAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGC‑GTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTC‑TGCTGGAA‑TTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATaaa                               <  2:211343/250‑1 (MQ=255)
                                                                                                                                                                                                                       gCAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGC‑GTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTC‑TGCTGGAA‑TTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTc                            <  1:245074/250‑1 (MQ=255)
                                                                                                                                                                                                                        cAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGC‑GTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTC‑TGCTGGAA‑TTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCg                           <  2:471152/250‑1 (MQ=255)
                                                                                                                                                                                                                           tGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGC‑GTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTC‑TGCTGGAA‑TTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGTTAAAGTCGACg                        <  2:65554/250‑1 (MQ=255)
                                                                                                                                                                                                                              cGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGC‑GTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTC‑TGCTGGAA‑TTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCt                     <  2:294257/250‑1 (MQ=255)
                                                                                                                                                                                                                                 gAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGC‑GTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTC‑TGCTGGAA‑TTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGAc                  >  1:472138/1‑250 (MQ=255)
                                                                                                                                                                                                                                    aaCAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGC‑GTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTC‑TGCTGGAA‑TTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACtta               >  2:83313/1‑250 (MQ=255)
                                                                                                                                                                                                                                           cAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGC‑GTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTC‑TGCTGGAA‑TTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTataat        <  1:451503/250‑1 (MQ=255)
                                                                                                                                                                                                                                                   aT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGC‑GTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTC‑TGCTGGAA‑TTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAgcgc  >  2:441957/1‑248 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                            
GCGCGGCAGTTATTGGAGAGCAACATCGCCGAGTTTGCCGCTTTGCAGGCTACCCGCGAAGATATCGTCAAAATGCGTCAGGCATTGCAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGAT‑CCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGA‑GTGGTTGGGCGATCACAAACAGATCCTCGCCGC‑GTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTC‑TGCTGGAA‑TTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGC  >  NZ_CP009273/4544693‑4545183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑