| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,594,167 | 0 | C | T | 42.0% | 78.6 / 36.9 | 69 | S64F (TCT→TTT) | bglJ | DNA‑binding transcriptional activator BglJ |
| Reads supporting (aligned to +/- strand): ref base C (7/33); new base T (29/0); total (36/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.54e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGT‑CAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGG > NZ_CP009273/4593918‑4594411 | aaTAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATtttttctttttc < 1:468044/250‑1 (MQ=255) aTAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTttttctttttct < 1:350327/250‑1 (MQ=255) aTAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTttttctttttct < 1:461456/250‑1 (MQ=255) gcgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCAt < 1:666535/250‑1 (MQ=255) gTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTGCCCTGTGCAGt > 2:114911/1‑250 (MQ=255) gTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTTTGCCATGAGGAGt > 1:526749/1‑250 (MQ=255) aaGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAgc < 1:149046/250‑1 (MQ=255) gagaACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTGCCATGAGAAGTgggggc > 1:112201/1‑245 (MQ=255) gaACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCag < 1:533000/250‑1 (MQ=255) acttaacttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag < 2:226943/250‑1 (MQ=255) aacttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAgg < 2:233965/249‑1 (MQ=255) aacttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGG‑CCATTTTTTTTTTTTTGCCATGAGAAGGGAGGGCGGAGgggg > 2:178099/1‑250 (MQ=255) ttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CCTTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCCGAGGGGGGtt > 1:238255/1‑250 (MQ=255) ttCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCtt > 1:570063/1‑250 (MQ=255) tttATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCCTTGAG‑AGGGAGCGGCGGGAGGGGTTATCtttgctg > 2:597341/1‑245 (MQ=255) tttATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCc‑t < 1:14634/249‑1 (MQ=255) tGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCc‑tgact < 2:245599/249‑1 (MQ=255) gCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CTTTTTTTTTTTTTTTTTCATGAGAAGTGGGGGCTCCGTGGGGTTATCTTTCC‑TTGCTGTACTcgcgtt > 1:469606/1‑248 (MQ=255) gCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGATTATTTTGCC‑TGACTGAACTGGCGA‑t > 1:175315/1‑250 (MQ=255) gAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTGCCCTGAGGAGTGAGCGCAGAGAGGGGTTATCTTGCC‑TGACTGAACTGGCGA‑TTa > 1:268487/1‑249 (MQ=255) acacAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTTCCATGAGAAGGGGGGGCAGAGAGGGATTATTTTTCC‑TTAATGAACTTGCGG‑TTAAGTTTCcg > 1:540794/1‑250 (MQ=255) aGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTa < 2:320929/250‑1 (MQ=255) cGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTTCCATTAGAAGTGAGCGGAGAGAGGGGTTATCTTTCC‑TGACTTAACTTGGGT‑TTAATGTTTCACGTAccc > 2:261615/1‑250 (MQ=255) cGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTAccc < 1:221204/250‑1 (MQ=255) gAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTTCCCTTAGAAGGGAGGGCCGAGAGGGGTTATCTTTCC‑TGACTGAACTTGCGG‑TTAAGTTTTCCCGTAcccc > 1:10720/1‑249 (MQ=255) gaagaGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTa < 1:600260/250‑1 (MQ=255) aagaGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTAg < 1:632422/250‑1 (MQ=255) gagaAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTAGtt < 2:53731/250‑1 (MQ=255) ataaaTGTCGCACTGAAAGAAAAAGTCGACATGAGGAGCATCTGGATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATATCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTGTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGAGTAGCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTGCCCGGC‑GTTTAGtga < 2:669939/248‑3 (MQ=255) agaAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTCTGCCATGAGAAGTGAGGGCCGAGAGGGGTTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTAGttt > 1:82712/1‑249 (MQ=255) cATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTGCCATTAGAAGTGGGGGCAGGGGGGGGTTTTCTTTCCTTGATTTAATTGGGGT‑TTAATTTTCC‑CGTACCCCGCGGTTTGGTTTTTggggttatgg > 2:158832/1‑243 (MQ=255) tAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTGTCTTATTCTGCCATGAGAAGTGAGCGCAGAGAGAGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTAGTTATTGCGGATGATGatat < 2:274713/250‑1 (MQ=255) gAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTAGTTATTGCGGATGATGATAtt < 2:680270/249‑1 (MQ=255) aaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCCGAGAGGGGTTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTTCCCGTACCCGGC‑GTTTTGTTATTGTGGATGATGGTATTg > 1:255498/1‑248 (MQ=255) gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTc < 1:407977/250‑1 (MQ=255) ggTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGTC‑TCACTGAACTGGGGA‑TTAAGTTTTCGCCGGCCCGGC‑GGTTTGTTTTTTGGGGTGATGGTAATTAAGCTCGGGTGGTTgggttctttt > 2:495764/1‑242 (MQ=255) ggTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTg < 2:83485/249‑1 (MQ=255) gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCa < 2:221217/249‑1 (MQ=255) gTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTTCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GGTTAGTTATTGCGGATGATGATATTTAAGCTCGGCTGATTGGTTCATTGTCGCCATCAcc > 1:95935/1‑250 (MQ=255) tttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTGCCCTTGGAAGTGAGGGCAGGGAGGGGTTATCTTGCC‑TGACTGAACTGGCGA‑ATTAGTTTTCGCGTACCCCGC‑GGTTTGTTATTGCGGGTGATGATATTGAAGCTCGGCTGATTGGTTTATTGTCGCCCTCACCg > 2:84844/1‑250 (MQ=255) tttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCg < 2:404851/250‑1 (MQ=255) tATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGGTTTTCTTTTC‑TGACTGAACTGGCGA‑TTATGATTCCGCGTACCCGGC‑GTTTAGTTATTTCGGATGATGGGATTTTAGATCGGGTGATTGGTTCTTTTTCGcctctccccct > 2:646715/1‑241 (MQ=255) aGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACg < 1:175520/250‑1 (MQ=255) aGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGAGGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCGGGACg < 2:380965/250‑1 (MQ=255) gAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACgg < 2:568137/250‑1 (MQ=255) aaaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGgt < 2:120712/250‑1 (MQ=255) tttGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTAt < 1:539624/250‑1 (MQ=255) tttGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTAt < 1:344636/250‑1 (MQ=255) tGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTGCCATGAGGAGTGAGCGCAGAGAGGGGTTTTCTTGCC‑TGACTGAACTGGCGG‑TTAAGTTTTCGCGTACCCGGC‑GTTTTGTTTTTGCGGATGATGATATTTAAGCTCGGCTGATTGGTTCATTTTCGCCATCACCGCTGGACGGGGGAttt > 1:127915/1‑249 (MQ=255) aaGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGA‑CATTTTTTTTTTTTCTGCCCTGAGTAGTGAGCGCAGAGGGGGGTTATCTTGCC‑TGAATGAAAAGGCGA‑TTAAAATTCCGCGGACCCGGC‑GGTTAGTTATTGCGGATGATGATATTGAAGCTCGGGTGGTTGGGTTATTGTTGCCATCACCGCTGGGAGGGGTGTTTAGTATAGGGT‑CAACGc > 2:518468/1‑250 (MQ=255) ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CCTTTTTTTTTTTTTTTCCCTGAGAAAGGAGGGCCGAGAGGGGTTTTCTTTCC‑CGACTGAACTTGCGG‑TTTAGGTTTCCCGGACCCGGC‑GCTTTGTTATTGCGGGTGATGATAATTAAAGTCGGGCGGTTTGTTTCTTTTCGCCATCACCCCTTGGCGGTGGTTTAAGTTAAGCGG‑CCAAGCTTgag > 2:609493/1‑250 (MQ=255) ccATACATATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTTCCATGAGAAGGGAGGGCCGAGAGGGGTTATCTTTCC‑TGACTGAACTGGCGG‑TTAAGGTTCCGCGGACCCGGC‑GGTTTGTTTTTTCGGGGTGTGATATTTAAGCTCGGCTGGTTTGTTCCTTTTTGCCATTCCCGCTGGGAGGGGTTTTTAGTAAAACggcccaggtgggg > 2:367588/1‑238 (MQ=255) aTACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CCTTTTTTTTTTTTTTGCCATGGGAAGTGGGCGCAGAGGGGGGTTTTTTTTCC‑TGGCTGAACTGGCGG‑TTTAATTTCCGCGGACCCCGC‑GGTTTGTTTTTGCGGGTGATGATATTTAAACTCGGCTGATTTGTTTCTTTTTGCCCTCCCCGCTGGGCGGGGTTTTAAGTAAAGCGG‑TAAAGCTGGAGAt > 2:686415/1‑250 (MQ=255) gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CTTTTTTTATTTTTTTGCCATGAGAAGTGAGCGCCGAGAGGGGTTATCTTTCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTTGTTATTGCGGATGGTGATATTTAAGCTCGGCTGATTGGTTTATTGTCGCCCTCACCGCTGGACGGGGTATTAAGTAAAGCGT‑CAACGCTGGAGATTTTTTATCAGGAActct > 1:589218/1‑250 (MQ=255) gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGT‑CAACGCTGGAGATTTTTCATCAGGAActct < 1:65448/250‑1 (MQ=255) tCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CCTTTTTTTTTTTTTTGCCCTGGGAAGTGAGCGCCGAGAGGGATTATCTTTCC‑TGACTGAACTGGCGG‑TTAAGTTTTCGCGTACCCGGC‑GTTTAGTTTTTTCGGGTGATGATATTTAAGCTCGGCTGGTTGGTTTATTTTCGCCCTCACCCCTGGGCGGGGTATTTAGTAAAAAGG‑CACAGCTGGGGATTTTTTATCCGGGAATCtttt > 2:510069/1‑250 (MQ=255) tCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCCGAGAGGGGTTTTCTTTCC‑TGGCTGAACTGGCGG‑TTAAGTTTTCGCGTAACCGGC‑GTTTAGTTATTTCGGGTGATGATATTTAAGGTCGGGTGGTTTGTTTATTTTTGCCCTCACCCCCGGGCGGGGTATTTAGTAAAAAGT‑TAACGGTGGAGGTTTTTTCTCAGGGCCTCtttt > 2:60460/1‑250 (MQ=255) cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTTCCATTAGGAGGGAGCGCAGAGAGGGGTTTTTTTTCC‑TGGCTTAACTGGGGATTTAAGTTTCC‑CGGACCCGGC‑GGTTTGTTATTTCGGGTGGTGATTTTTAAGGTCAGGTGATTTGTTTTTTGTTGGCCTCCCCCCCTGGCGGGGTTTTAAATAAAAGGTCCACCCCGGGGGATTTTTC‑TCAAGAACTtttttt > 2:529284/1‑250 (MQ=255) aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTCTGCCATGAGAAGTGGGCGCAGAGAGGGATTATCTTTCC‑TGACTGAAATGGCGG‑TTAAGTTTTCGCGTACCCGGC‑GTTTTGTTATTTCGGATGATGATATTTAAGCTCGGCTGGTTTGTTCATTTTCGCCCTCACCCCTGGGCGGGGTATTAAGTAAAACGT‑CAACGCTGGAGAATTTTTATCAGGAACTCtttttt > 1:131174/1‑249 (MQ=255) tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTGCCCTGAGAAGGGAGCGCAGAGAGGGGTTATCTTTCC‑TGGCTGAACTGGCGA‑TTAAGTTTTCGCGTACCCCGC‑GTTTTGTTATTTCGGGTGATGGAATTTAAGCTCGGCTGTTTGGTTCATTTTTGCCATCACCGCTGGGCGGTGGATTAAGTAAAGCGT‑CAACACTGGAGATTTTTTATCAGGAAATCttttttt > 2:469485/1‑248 (MQ=255) cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTGCCATGGGAAGGGAGCGCAGAGAGGGGTTATCTTTCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCCGC‑GTTTTGTTATTGCGGGTGATGATATTTAAGGTCGGCTGATTGGTTCCTTTTCGCCCTCACCGCTGGACGGGGTATTAAGTAAAGCGT‑CAAAGCTGGAGATTTTTCATCAGGAACTCTTTTTTTCAt > 1:460012/1‑250 (MQ=255) gATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGT‑CAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTaa < 1:119585/250‑1 (MQ=255) tGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGT‑CAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAAt < 1:41437/250‑1 (MQ=255) ccATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTTTGCCCTGAGAAGGGAGCGCAGAGAGGGGTTATCTTGTC‑TGACTGAAATGGCGA‑TTAAGTTTTCGCGTACCCGGG‑GGTTTGTTATTGGGGGTGATGATATTTAAGGTCGGCTGGTTGGTTTATTGTTGCCATCACCGCTGGGAGGGGGATTAAGTAAAGGGG‑CAACGCTGGGGATTTTTTATCAGGAAATCTTTTTGTCATTTAATgg > 1:105012/1‑250 (MQ=255) aTGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTTTTTTTTTGCCCTGAGAAGAGAGGGGAGAGAGGGGGTTTCTTGCC‑TGACTGAACTGGCGG‑TTTAGGTTTCGCGTACCCCGG‑GGTTTGTTTTTTTGGAGGGTGGTATTTAAGATCCGCTGGTTGGTTCATTGTTGCCCTCACCGCTGGGCGGTGTATTAAGTTAAGCAG‑CAACGCTGGAGGATTTTTATCCAGGACTCCTTTTTTTATTATATGgtg > 2:349320/1‑250 (MQ=255) gcgGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGT‑CAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACg < 1:245121/249‑1 (MQ=255) gTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGATAAGTGAGCGCAGAGAGGGATTTTCTTGCC‑TTACTGAACTGGCGA‑TTAAGCTTCCGCGTACCCGGC‑GTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGT‑CAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGcga < 2:549720/248‑1 (MQ=255) tttGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGT‑CAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGcgaccg < 2:109309/250‑1 (MQ=255) gcggT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGT‑CAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGcgaccgaccgac < 2:268487/250‑1 (MQ=255) gcggT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGT‑CAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGcgaccgaccgac > 2:680892/1‑250 (MQ=255) t‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGT‑CAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGaa > 1:403989/1‑250 (MQ=255) aTTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGT‑CAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAAc < 1:26616/249‑1 (MQ=255) tttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGT‑CAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTgg > 1:500207/1‑250 (MQ=255) | AATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGT‑CATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGC‑GTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGT‑CAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGG > NZ_CP009273/4593918‑4594411 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |