Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,336,711	G→T	E173* (GAG→TAG)	lptC →	LPS export ABC transporter periplasmic protein LptC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,336,711	0	G	T	100.0%	286.0 / NA	72	E173* (GAG→TAG)	lptC	LPS export ABC transporter periplasmic protein LptC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (46/26);  total (46/26)

ATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCGAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTCATCGTCACCCAGGGCACCATCAAAATTAATGCCGACAAAGTGGTC  >  NZ_CP009273/3336465‑3336957                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       aTCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGc                                                                                                                                                                                                                                                     >  1:124932/1‑250 (MQ=255)     cGACATGGTCCGTAAAGGCAGATAAAGCCAAGCTGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCCCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGAt                                                                                                                                                                                                                                                 >  2:466383/1‑250 (MQ=255)      gACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGAtt                                                                                                                                                                                                                                                >  2:181317/1‑250 (MQ=255)            gTCCGTAAAAGCAGATAAAGCCAAGCTGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGGCGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGaaaa                                                                                                                                                                                                                                           <  2:356601/249‑1 (MQ=255)                         gATAAAGCCAAGCTGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCt                                                                                                                                                                                                                             <  2:39669/250‑1 (MQ=255)                           tAAAGCCAAGCTGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTAt                                                                                                                                                                                                                           <  2:521026/250‑1 (MQ=255)                            aaaGCCAAGCTGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTAt                                                                                                                                                                                                                           >  2:11043/1‑249 (MQ=255)                             aaGCCAAGCTGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGa                                                                                                                                                                                                                         <  1:357383/250‑1 (MQ=255)                                ccAAGCTGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAAt                                                                                                                                                                                                                      >  1:437829/1‑250 (MQ=255)                                     cTGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCaa                                                                                                                                                                                                                  >  2:275469/1‑249 (MQ=255)                                      tGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCaaa                                                                                                                                                                                                                 >  2:602553/1‑249 (MQ=255)                                           aaTGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAaaacaaa                                                                                                                                                                                                            >  2:561211/1‑249 (MQ=255)                                            aTGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAaaacaaaca                                                                                                                                                                                                          <  1:5364/250‑1 (MQ=255)                                               aCCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCACGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAaaacaaacaaac                                                                                                                                                                                                       >  1:692712/1‑250 (MQ=255)                                                ccGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACt                                                                                                                                                                                                      >  1:275658/1‑250 (MQ=255)                                                    aTGCTCTATTTATATGGACACGTTGAAGTCAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGGGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGc                                                                                                                                                                                                  >  1:677677/1‑250 (MQ=255)                                                     tGCTCTATTTATATGGACACGTTGAAGTCAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGcc                                                                                                                                                                                                 >  1:47863/1‑250 (MQ=255)                                                      gCTCTATTTATATGGACACGTTGAAGTCAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTAACTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCt                                                                                                                                                                                                >  2:280629/1‑250 (MQ=255)                                                          tATTTATATGGACACGTTGAAGTCAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATGTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAGCAAACAAACTCAGCCTTa                                                                                                                                                                                              <  1:517834/248‑1 (MQ=255)                                                            tttATATGGACACGTTGAAGTCAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATc                                                                                                                                                                                           <  2:634118/249‑1 (MQ=255)                                                            tttATATGGACACGTTGAAGTCAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCt                                                                                                                                                                                          >  2:35100/1‑250 (MQ=255)                                                                tataGACACGTTGAAGTCAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAAACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTtgtg                                                                                                                                                                                      <  2:485192/250‑1 (MQ=255)                                                                     acacGTTGAAGTCAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGc                                                                                                                                                                                 >  1:13565/1‑250 (MQ=255)                                                                         gTTGAAGTCAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGc                                                                                                                                                                             >  2:30056/1‑250 (MQ=255)                                                                              aGTCAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACt                                                                                                                                                                        >  1:478160/1‑250 (MQ=255)                                                                                 cAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCt                                                                                                                                                                     >  1:138556/1‑250 (MQ=255)                                                                                  aaCGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTg                                                                                                                                                                    >  2:421836/1‑250 (MQ=255)                                                                                         ttGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGgccgc                                                                                                                                                               >  2:236812/1‑248 (MQ=255)                                                                                         ttGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGgccgc                                                                                                                                                               >  1:15298/1‑248 (MQ=255)                                                                                               cAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTc                                                                                                                                                       >  2:237786/1‑250 (MQ=255)                                                                                               cAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTc                                                                                                                                                       <  1:181320/250‑1 (MQ=255)                                                                                                   ctctCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGc                                                                                                                                                   <  1:626134/250‑1 (MQ=255)                                                                                                         aCTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGc                                                                                                                                             >  1:45807/1‑250 (MQ=255)                                                                                                          cTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGcc                                                                                                                                            <  1:488220/250‑1 (MQ=255)                                                                                                                 gAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCg                                                                                                                                     >  1:507314/1‑250 (MQ=255)                                                                                                                      acgacgGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGgaga                                                                                                                                 <  1:625611/249‑1 (MQ=255)                                                                                                                           ggATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGa                                                                                                                           <  1:563462/250‑1 (MQ=255)                                                                                                                              tAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCa                                                                                                                        <  2:467865/250‑1 (MQ=255)                                                                                                                                       gATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCca                                                                                                               >  2:327956/1‑250 (MQ=255)                                                                                                                                       gATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCca                                                                                                               >  2:173520/1‑250 (MQ=255)                                                                                                                                                 gTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAAt                                                                                                       <  2:116233/248‑1 (MQ=255)                                                                                                                                                  tGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCgg                                                                                                    <  2:655342/250‑1 (MQ=255)                                                                                                                                                       cAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAg                                                                                               >  2:582674/1‑250 (MQ=255)                                                                                                                                                        aGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAg                                                                                               >  2:172024/1‑249 (MQ=255)                                                                                                                                                            tGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCgg                                                                                                                                    >  1:417002/1‑208 (MQ=255)                                                                                                                                                             gTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGga                                                                                                                                   <  2:417002/208‑1 (MQ=255)                                                                                                                                                              ttACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAAtctc                                                                                        >  2:479282/1‑250 (MQ=255)                                                                                                                                                               tACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATctct                                                                                       >  2:616802/1‑250 (MQ=255)                                                                                                                                                                 ccTCTGAAGACCTCGTCACGTTATACGGAAAAACATTTAACTCCAGCGGTCAGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATctctg                                                                                      <  2:25903/249‑2 (MQ=255)                                                                                                                                                                    ctGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGata                                                                                  >  1:43536/1‑250 (MQ=255)                                                                                                                                                                     tGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGatat                                                                                 >  2:461862/1‑250 (MQ=255)                                                                                                                                                                       aaGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGatat                                                                                 <  1:327956/248‑1 (MQ=255)                                                                                                                                                                       aaGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATg                                                                                <  1:368386/249‑1 (MQ=255)                                                                                                                                                                            cTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGc                                                                          <  1:558786/250‑1 (MQ=255)                                                                                                                                                                              cGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCaa                                                                        >  1:324015/1‑250 (MQ=255)                                                                                                                                                                                    gTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTT‑TGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGt                                                                  >  1:81867/1‑249 (MQ=255)                                                                                                                                                                                       ataCGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTa                                                                <  1:185628/249‑1 (MQ=255)                                                                                                                                                                                        taCGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAcc                                                              >  1:582386/1‑250 (MQ=255)                                                                                                                                                                                        taCGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAc                                                               <  2:324015/249‑1 (MQ=255)                                                                                                                                                                                           ggAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCtt                                                            >  2:191976/1‑249 (MQ=255)                                                                                                                                                                                              acaacaTTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTAcc                                                        <  1:479250/250‑1 (MQ=255)                                                                                                                                                                                                 acaTTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCgg                                                      >  2:454134/1‑249 (MQ=255)                                                                                                                                                                                                   aTTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTaa                                                   >  2:478094/1‑250 (MQ=255)                                                                                                                                                                                                           ccAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTCATCg                                           >  1:408418/1‑250 (MQ=255)                                                                                                                                                                                                             aGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTCATCGt                                          >  1:200979/1‑249 (MQ=255)                                                                                                                                                                                                                 gTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTCATCGTCAcc                                      >  1:450591/1‑249 (MQ=255)                                                                                                                                                                                                                          tGCGCGGCAACTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTCATCGTCACCCAGGGCACCa                            >  1:435329/1‑250 (MQ=255)                                                                                                                                                                                                                                  aaCTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTCATCGTCACCCAGGGCACCATCAAAAt                     >  1:139138/1‑249 (MQ=255)                                                                                                                                                                                                                                  aaCTTACGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTCATCGTCACCCAGGGCACCATCAAAAt                     >  1:49195/1‑249 (MQ=255)                                                                                                                                                                                                                                        cGCAGCAAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTCATCGTCACCCAGGGCACCATCAAAATTAATGcc              >  1:620238/1‑250 (MQ=255)                                                                                                                                                                                                                                           agcaAGAACGCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTCATCGTCACCCAGGGCACCATCAAAATTAATGCCGAc           <  1:652373/250‑1 (MQ=255)                                                                                                                                                                                                                                                    gCCTAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTCATCGTCACCCAGGGCACCATCAAAATTAATGCCGACAAAGTGGTc  <  2:286675/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       ATCCCGACATGGTCCGTAAAAGCAGATAAAGCCAAGCTGACCAATGACCGGATGCTCTATTTATATGGACACGTTGAAGTCAACGCACTTGTGCCAGACTCTCAACTTCGCAGAATCACGACGGATAACGCGCAGATCAATCTGGTGACGCAGGATGTTACCTCTGAAGACCTCGTCACGTTATACGGAACAACATTTAACTCCAGCGGTCTGAAAATGCGCGGCAACTTACGCAGCAAGAACGCCGAGCTGATTGAAAAGGTTAGAACATCCTATGAAATTCAAAACAAACAAACTCAGCCTTAATCTTGTGCTTGCCAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTCATCGTCACCCAGGGCACCATCAAAATTAATGCCGACAAAGTGGTC  >  NZ_CP009273/3336465‑3336957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑