Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP00927315,3950GA39.5% 56.6 / 16.5 43intergenic (+97/‑50)dnaJ/BW25113_RS00080molecular chaperone DnaJ/IS4‑like element IS421 family transposase
Reads supporting (aligned to +/- strand):  ref base G (3/23);  new base A (17/0);  total (20/23)
Fisher's exact test for biased strand distribution p-value = 2.71e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATA‑TTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCT‑AACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTGAAGTCACTGCATGGG  >  NZ_CP009273/15147‑15634
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 
tcgAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAgc                                                                                                                                                                                                                                                  <  1:48755/250‑1 (MQ=255)
     acacCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTaa                                                                                                                                                                                                                                             <  2:82103/250‑1 (MQ=255)
            tAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAgg                                                                                                                                                                                                                                      <  2:188920/250‑1 (MQ=255)
                 cTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACCCCCACAAGAGCTAACTTAAggggttt                                                                                                                                                                                                                                 >  1:488810/1‑246 (MQ=255)
                    aaCGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTtgtg                                                                                                                                                                                                                                <  2:480349/248‑1 (MQ=255)
                                     gctgctGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATGACACCCCTCAAAGCTACATTAAGGGGTGTGGGTTTACGCCTGACATGAt                                                                                                                                                                                                             >  2:31634/1‑250 (MQ=255)
                                                 gctgcaagaAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGa                                                                                                                                                                                                 <  2:3949/250‑1 (MQ=255)
                                                 gctgcaagaAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGAACAATCCCCTAAACGCTCCCTTAATGGGGGGGGTGTTACGCCCGATATTATTTaaatatccgg                                                                                                                                                                                                 >  2:522705/1‑242 (MQ=255)
                                                   tgcaagaAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGGACTCCCCTAAACGCCAACATTAGAGGTGTGGGATTATGGCTGGTATGAtttttaatgccggt                                                                                                                                                                                                >  1:296730/1‑238 (MQ=255)
                                                      aagaAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACACCCCTAAAAGCGAAATTAAGGGGTGTGGGATTATGCCTGATATGATTTTACGTGGCGATGAAt                                                                                                                                                                                            >  1:5831/1‑250 (MQ=255)
                                                        gaAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGGACACCCCAAAACGCGAAACTAAAGGGTGTGGTGTTATGCCTGGTATGATTTTAAGTGGCGGTGaaat                                                                                                                                                                                           >  2:326802/1‑247 (MQ=255)
                                                            gCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTGACTCCCCTAAACGCTAACTTAAGGGGTGTGGGATTACGCCTGGTATGATTTTAAGTGCCGATGGATTaattt                                                                                                                                                                                      >  1:542987/1‑246 (MQ=255)
                                                            gCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTActct                                                                                                                                                                                      <  1:541082/250‑1 (MQ=255)
                                                                     gCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAAc                                                                                                                                                                             <  2:110160/250‑1 (MQ=255)
                                                                            cGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCCTAAACGCTAAATTTAAGGTTGTGGTATTTCGCCCGATATTATTTTACGTGCCGAGGAATTACTCTCACGATAAATGGTCAg                                                                                                                                                                      >  1:491200/1‑250 (MQ=255)
                                                                              gCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTTAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCa                                                                                                                                                                    <  2:452374/250‑1 (MQ=255)
                                                                               cGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCaa                                                                                                                                                                   <  2:517100/250‑1 (MQ=255)
                                                                                                   cAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCATAAACGCTAACTTAAAGGGTGTGGGATTTCGCCTGATATGATTTTACGTGCCGATGAATTAATCTCACGATAAATGGTCAGCAATTCTTGCCCATA‑TTTGTaaa                                                                                                                                              >  1:304456/1‑249 (MQ=255)
                                                                                                    aaaGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACCCCCCCAAACGCGAAACTAAAGGGTGTGGGGTTATGGCTGGTATGATTTTACGAGGCGGTGGATTAATCTCACGCAAAATTGGGAGGAATTTTGGCCCCTA‑TTGGGaaac                                                                                                                                             >  2:87179/1‑248 (MQ=255)
                                                                                                    aaaGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATA‑TTGGTAAGc                                                                                                                                             <  1:574219/250‑1 (MQ=255)
                                                                                                     aaGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATA‑TTGGTAAGcc                                                                                                                                            <  1:387221/250‑1 (MQ=255)
                                                                                                     aaGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATA‑TTGGTAAGcc                                                                                                                                            <  2:535917/250‑1 (MQ=255)
                                                                                                       gagCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGAACAATCCCCTAAACGCTAACTTAAGGGGTGTGGGGTTTAGCCCGATATGATTTAAAGTGCCGATGAATTACTTTCACCATAAATGGGCAGCAATTCTGGCCCCAA‑TTGGTAAGccc                                                                                                                                           >  1:351178/1‑249 (MQ=255)
                                                                                                          cttcttTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATA‑TTGGTAAGCCCgaag                                                                                                                                       <  1:46661/250‑1 (MQ=255)
                                                                                                             cttTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATA‑TTGGTAAGCCCgaagaa                                                                                                                                     <  1:55519/249‑1 (MQ=255)
                                                                                                              tttGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACCCCCATAAACGCCAACTTAAAGGGTGTGGGATTTAGCCCGATATGATTTTAACTGCCGGTGAATTACTCTCACGATAAATGGGCAGCAATTCTTGCCCCTA‑TTTGGAAACCCgaagaaat                                                                                                                                   >  1:586865/1‑248 (MQ=255)
                                                                                                                        tgAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATA‑TTGGTAAGCCCGAAGAACTGGATACTTCg                                                                                                                         <  1:23192/250‑1 (MQ=255)
                                                                                                                                   tttGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCCTAAACGCTAACTTTAGGGGTGTGGTATTATGCCTGATATTATTTTACGTGCCGGTGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCCTA‑TTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGc                                                                                                              >  1:480043/1‑250 (MQ=25)
                                                                                                                                       acgacCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATA‑TTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCggg                                                                                                          <  1:229985/250‑1 (MQ=255)
                                                                                                                                       acgacCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGAGCACTCCCCTAAACGCCAACTTAAGGGGTGTGGGATTAAGCCTGATATGATTTTAAGTGCCGATGAATTACTCTCACGATAAATGGGCAGCAATTCTTGCCCACA‑TTGGTAAGCCCGAAGAAATGGATACTTCGGCACGTAATGCCggg                                                                                                          >  1:8733/1‑250 (MQ=255)
                                                                                                                                                  cGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAACGCTAAATTAAAGGGTGTGGGATTTCGCCTGATATTATTTTAAGGGCCGATGAAATAATCTCACGATAAATTGTCAGCAATTCTGGCCCCTA‑TTTGTAAACCCGAAAAAATGGGTAATTCGGGCCGCAATGCCGGGGCTCT‑AAcccc                                                                                              >  1:146398/1‑249 (MQ=255)
                                                                                                                                                         cTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGGACACCCCTAAAAGCTAACCTAAAGGGTGTGGGGTTATGCCCGGTATGATTTTAAGTGCCGGTGAAATAATCTCACGATAAAAGGTCAGCAAATCTGGCCCCTA‑TTTGTAAAACCGCAGAAAAGGATACTTCGGCACCGAATGCCGGGGGTCT‑TACCccccgccgc                                                                                       >  2:81363/1‑250 (MQ=255)
                                                                                                                                                              cAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATA‑TTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCT‑AACCCGCCGCCGCGAAAt                                                                                  <  1:75377/250‑1 (MQ=255)
                                                                                                                                                                    ccTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACTCCCCTAAACGCTAACTTAAGGGTTGTGGTGTTATGCCTGATATGATTTTACGTGCCGATGAATTACTCTCACGATAAAAGGTCAGCAAATCTGGCCCACA‑TTGGTAAACCCGCAGAAAAGGATAATTCGGCACGTAAAGCCGGGGCGCTCAACCCCCCCCGCCGAAATACggg                                                                             >  2:127451/1‑248 (MQ=255)
                                                                                                                                                                    ccTACCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGCTGAAGATATGCGAGCCCCTGTAAAGTGGCGGGGATCACTCCTATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCCCGATAACTGGTCAGCAATTCTGGCCCATA‑TTGGTAAGCCCGAAGAACTGGATACTACGGCACGTAATGCCGGGGCTCT‑AACCCGCCGCCGCGAAATTCGTGa                                                                            <  2:142602/250‑1 (MQ=255)
                                                                                                                                                                        cccGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACCTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATA‑TTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCT‑AACCCGCCGCCGCGAAATTCGTGAtgct                                                                        <  1:55305/250‑1 (MQ=255)
                                                                                                                                                                         ccGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGAGCACTCCCACAAACGCCAACCTTAGGGGTGGGGGGTTAAGCCTGGTAAGATTTAACGTTCCGCTGAAATACTCTCCCGATAAATGGTCAGCAATTCTTGCCCCTATTTGGAAAGCCC‑CAGAAACAGATAATTCCGCACGTAATGCCGGGGCGCT‑AACCCCCCCCCCCCGAAATCGTGAtgctg                                                                       >  2:74222/1‑250 (MQ=255)
                                                                                                                                                                                     cTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATA‑TTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCT‑AACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTAc                                                           <  1:553381/250‑1 (MQ=255)
                                                                                                                                                                                                       cGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATA‑TTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCT‑AACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTAc                                          <  1:111732/249‑1 (MQ=255)
                                                                                                                                                                                                              gATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATA‑TTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCT‑AACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCgg                                  <  1:538104/250‑1 (MQ=255)
                                                                                                                                                                                                                     gAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATA‑TTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCT‑AACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATg                           <  2:351178/250‑1 (MQ=40)
                                                                                                                                                                                                                                   tGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGGTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTATGGCCCATA‑TTGGTAAGCCCGAAGAACTGGATACTTCGGCAAGTAATGCCGGGGCTCT‑AACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTGAAGt             >  2:513909/1‑250 (MQ=18)
                                                                                                                                                                                                                                     gCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATA‑TTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCT‑AACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTGAAGTCa           <  1:335157/250‑1 (MQ=32)
                                                                                                                                                                                                                                              aCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATA‑TTGGTAAGCCCGAAGAACTGAATACTTCGGCACGTAATGCCGGGGCTCT‑AACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTGAAGTCACTGCATggg  >  2:129545/1‑250 (MQ=11)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 
TCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATA‑TTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCT‑AACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTGAAGTCACTGCATGGG  >  NZ_CP009273/15147‑15634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑