Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,845,013	0	T	G	45.6%	73.5 / 24.6	57	G218G (GGT→GGG)	hypB	hydrogenase nickel incorporation protein HypB
Reads supporting (aligned to +/- strand):  ref base T (6/25);  new base G (26/0);  total (32/25)
Fisher's exact test for biased strand distribution p-value = 7.52e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GAAGGCGACCAGCAAACCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCG  >  NZ_CP009273/2844771‑2845251                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               gAAGGCGACCAGCAAACCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTTCGTTACCGGAGGGGGAGAc                                                                                                                                                                                                                                          >  2:264841/1‑249 (MQ=255)   aGGCGACCAGCAAACCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTTCTGTTCCGACGGGGAAGACaaa                                                                                                                                                                                                                                       >  2:583603/1‑250 (MQ=255)                    tGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TGTTCTCTACCGACGAGGACAACAAACCACTGGAATATCCGc                                                                                                                                                                                                                       >  2:299197/1‑249 (MQ=255)                      aaCGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCat                                                                                                                                                                                                                     <  1:1598/249‑1 (MQ=255)                          aTGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGAC‑AACCACTGAAATATCCGCATATGttt                                                                                                                                                                                                               <  1:110148/250‑1 (MQ=255)                           tGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTTCCTTTCCGAAAGGGGAGACAAAACACTGAAAAATCCCCATATGttt                                                                                                                                                                                                               >  1:207890/1‑250 (MQ=255)                                   gcaTTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTTCGTTACCGAAGGGGAAGACAAACCACTGAAATATCCGCATATGTTTgccgccgc                                                                                                                                                                                                       >  1:447955/1‑250 (MQ=255)                                        cgcgCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCg                                                                                                                                                                                                   <  2:255960/249‑1 (MQ=255)                                         gcgcTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTTCGTTTCCGAAGGGGAAGACAAACCACTGAAATATCCGCATATGGTTTCCGCCGCCTCGCt                                                                                                                                                                                                 >  1:320509/1‑250 (MQ=255)                                           gcTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑CTTCCCGTACCGGAGGTGGAGACAAACCACTGAAATATCCGCATAAGGTTGTCGCCCCCTCGCTgt                                                                                                                                                                                               >  2:199100/1‑249 (MQ=255)                                               ccGGCCCACCAGCGATTCAGGTGAACCCCGGTAAAGGCTGCCATCTTGACGCACAGATGATGGCCGACGCAGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGAtg                                                                                                                                                                                             <  2:28826/248‑1 (MQ=255)                                                   cacaCCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTc                                                                                                                                                                                       <  1:92738/250‑1 (MQ=255)                                                         aGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACaaa                                                                                                                                                                                 <  1:275518/250‑1 (MQ=255)                                                         aGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCCGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGAAAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTcaacaa                                                                                                                                                                                  >  1:497904/1‑249 (MQ=255)                                                            gATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCAGGGG‑TTTCCGTTTCCCAAGGGGAAGACAAACACCATAAAAATCCGCATATGTTTGCCCCCGCCTCCCCGGTGCTGGTCCACAAAGtt                                                                                                                                                                              >  2:462331/1‑250 (MQ=255)                                                                 aGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGAcc                                                                                                                                                                          <  1:594217/249‑1 (MQ=255)                                                                  ggTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTTCCTTTTCGAAGGGTAAAACAAAACAACGTAATATACCCCTTTGTTTTCCGCCGCCTCCCTGCTGCCGCTCAACAAAGTTGatccg                                                                                                                                                                        >  1:36314/1‑246 (MQ=255)                                                                      aaCACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTg                                                                                                                                                                     <  1:201208/249‑1 (MQ=255)                                                                          ccGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTa                                                                                                                                                                <  1:487731/250‑1 (MQ=255)                                                                             gTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTAtct                                                                                                                                                             <  2:28733/250‑1 (MQ=255)                                                                                    cTGCCAGCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGTAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGTGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACttt                                                                                                                                                      <  2:465511/250‑1 (MQ=255)                                                                                       ccATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTg                                                                                                                                                     <  1:532847/248‑1 (MQ=255)                                                                                        cATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACg                                                                                                                                                  <  1:100998/250‑1 (MQ=255)                                                                                         aTCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTTCCTTTACC‑AAGGGGAAGAAAAAACACTGAAAAATCCCCCTATGTTTTCCCCCCGCCCGCTTGAGCTCGTCCACAAAATTTACCCGTTGCCGTATCTCAACtttttcct                                                                                                                                                 >  1:356365/1‑245 (MQ=255)                                                                                                          atTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGG‑TTTCCGTTTACGGAGGGGGAGGAAAACCACTGAAATAAACGCCTATGTTTGCCGCCGCCCCGCTGGTGCTTCTCCACCAAGATGAACTGTTGCCGGATCTCACCTTTTACCTTTAAAAggtgatcccct                                                                                                                                >  2:427549/1‑240 (MQ=255)                                                                                                           ttGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGGGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCCCGCTGATGCTGCTCAACAAAAGTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTg                                                                                                                               >  1:191827/1‑250 (MQ=255)                                                                                                                gACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGGGAAGACAAACCAATGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAATTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGGGCATCGCCTGCGccc                                                                                                                          >  1:192085/1‑250 (MQ=255)                                                                                                                       cACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGt                                                                                                                   <  1:279899/250‑1 (MQ=255)                                                                                                                                  ccACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGGGAAGACAAACCACTGAAATATCCGCATATGTTTTGCGCCGCCCCGCCGATGCCGCCCCACAAAGTTGACCTTTTGCCGTATTTCAACTTTTTCGTTTAAAAGTGCCTCGCCTGCCCCCCCCAAGTCAAACCCGaa                                                                                                         >  1:410039/1‑249 (MQ=255)                                                                                                                                      tGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTTCGTTTCCGAAGGTGAAGACAAAACACTGAAATATCCGCATATGTTTTCAGCCGCCTCGCTGATGCTGCTCAACAAAATTTACCTGTTTCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTg                                                                                                     >  1:405852/1‑249 (MQ=255)                                                                                                                                        gACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGa                                                                                                    <  1:383627/248‑1 (MQ=255)                                                                                                                                         aCGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAat                                                                                                 <  1:551031/250‑1 (MQ=255)                                                                                                                                           gATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTTCGTTACCGAAGGGGAAGACAAACCACTGAAATATCCGCCTATGTTTTCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGCATCTCCACTTTTACGTTGAAAAGTGCATCGCCCGCGCCCGCGAAGTCAATCCAGAAATTGAAatca                                                                                               >  1:73113/1‑250 (MQ=255)                                                                                                                                              aaCGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTTCGTTACCGAAGGGGAAGACAAAACACTGAAAAATTCGCATATTTTTGCCGCCGCCCCGCTGGTGCTGCTCCACAAAAGTTACCTGTTGCCGTATCTCCACCTTTACGTTGAAAAGAGCATCGGCTGCGCCCCCCGAGGCCAACCAAAAATTGAAAACATcc                                                                                            >  2:189590/1‑250 (MQ=255)                                                                                                                                               aCGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATcc                                                                                            <  2:215556/249‑1 (MQ=255)                                                                                                                                               aCGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCt                                                                                           <  1:309214/250‑1 (MQ=255)                                                                                                                                                cagttttCTGTTTCTCGACAATGTTGGCACCCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCtt                                                                                          <  2:266138/245‑1 (MQ=255)                                                                                                                                                cGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGGAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAATTGACCTGTTGCCGTATCTCAACTTTTACGTTTAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTTAAATCATCCtt                                                                                          >  1:533049/1‑250 (MQ=255)                                                                                                                                                cGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAACGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCtt                                                                                          <  2:355780/250‑1 (MQ=255)                                                                                                                                                     ttCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTTCCTTTCCGAAGGGGGAGAAAAAACAATGAAAAATCCGCCTATGTTTTCCGCCGCCCCGCCGGTGCTGCTCAACAAAAATGACCTGTTGCCGTATCTCAACTTTTACGTTGAAAAGTGCATCGCCTCCGCCCCCGCAGGCAATCCCGAAATTGAAAATATCCttttttt                                                                                     >  2:316199/1‑249 (MQ=255)                                                                                                                                                        tGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGGGAAGACAAAACACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTTACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGc                                                                                  >  1:389789/1‑250 (MQ=255)                                                                                                                                                              tCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAg                                                                            <  1:344956/250‑1 (MQ=255)                                                                                                                                                                     cGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGGGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAATTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAgcggcg                                                                       >  1:440761/1‑248 (MQ=255)                                                                                                                                                                      gTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAg                                                                    <  2:351850/250‑1 (MQ=255)                                                                                                                                                                             aCCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGa                                                             <  1:462331/250‑1 (MQ=255)                                                                                                                                                                               cTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTTCCGAAGGGGAAGACAAAACACAGAAATATCCGCCCATGTTTTTCGCCGCCCCGCCCGTGCTGCTCCAAAAAATTGTCCTGTTGCCGTATCTCCACATTGCCGGTGAAAAATGCATCGCCCGCGCCCGCGAAGACAAACCCGAAATTTAAATAATCCTTTTTTCCTCCCCCCCCCGCGGAGGGCGGGAcc                                                           >  2:417939/1‑250 (MQ=255)                                                                                                                                                                                tCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTTCGTTTCCGAAGGGGAAGAAAAACCACTGAAATATCCGCCTATGTTTTCCGCCGCCTCCCTGATTCTGCCCAACAAAAGTGGCCTGTTGCCGTATCTCCACTTTGACGTTGAAAAGTGGATAGCCTGCGCCCGCGAAGTCAATCCAGAAATTTAAATCATTCTTTTTTTCCCCACCAGCGGCGGAGGGGTGGaa                                                            >  2:388880/1‑247 (MQ=255)                                                                                                                                                                                  gTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTTCCGCAGGGGAAGACAAACCACCTAAAAATCCCCATATTTTTTCCCCCCCCTCGCTGATGGGTCCCAACAAAGGTTACCCTTTTCCGTGTCTCAAATTTTGCGTTGGAAAGTGCCTCGCCCGCGCCCCCGAAGTCAATCCCGAAATTGAAATCATCCTTTTTTTCGCCAACACCGGCGAAaggggtgacca                                                          >  2:45588/1‑240 (MQ=255)                                                                                                                                                                                    aTGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTTCGTTTCCGAAGGGGGAGACAAACCACTGAAAAATCCGCAAATGTTTGCCGCCCCCCCCCTGGTGCTGCTCAACAAAATTGACCTGTTGGCGTATCTCAACTTTTACGTTGAAAAATGCATCGCCTGCGCCCGCGACGTCAATCCCGAAAATGAAATCATCCTTATTTCCCCCACCCGCGGCGAAGGGAGGGGccccagg                                                      >  2:416641/1‑245 (MQ=255)                                                                                                                                                                                             cAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGGGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCCCGCTGATGCTGCTCAACAAAATTGACCTGTTGCCGTATCTCAACTTTTACGTTGGAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGGTGGACCAGTGGCTGAActgg                                             >  1:27821/1‑250 (MQ=255)                                                                                                                                                                                                  tCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTAACGCAGGGGGAGAAAAAACAATGAAAAATACGCATATGTTTGTCGCCCCCCCGCTGATGCTGCTCAACAAAATTTACCTGTTGCCGTATCTCAAACTTTACGTTGAAAAAAGCATCTCCTCCGCCCCCGAAACCAATCCACAAATTGAAATCATCCTTATTTCCGGCCCCAACGGGGGAGGGAGGGAACAGTGGTTGGAcctgctg                                          >  2:505859/1‑248 (MQ=255)                                                                                                                                                                                                     aTCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTTCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGgaga                                      >  1:77285/1‑249 (MQ=255)                                                                                                                                                                                                      tctcGGTGAAAAACACAAAGTGGCGGTGC‑TTTTCGTTACCGAAGGGGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCAACGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGGGaca                                    >  1:24844/1‑250 (MQ=255)                                                                                                                                                                                                        tcGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGGGAAGACAAACCACTGAAATATCCGCCTATGTTTTCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTTACGTTGAAAAGTGCATCGCCTGCGCCCGCGCAGTCAATCCCGAAATTGAAATCATCCTTATTTTCGCCACCAGCGGCGAAGGGGTGGACCAGTGGCTGAACTGGCTGGAGaca                                    >  1:380014/1‑248 (MQ=255)                                                                                                                                                                                                                        aGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGTCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCg                  <  1:382769/250‑1 (MQ=255)                                                                                                                                                                                                                             cGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTccc             <  1:376719/250‑1 (MQ=255)                                                                                                                                                                                                                                          ttACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCg  >  1:163555/1‑249 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               GAAGGCGACCAGCAAACCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTGAACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGC‑TTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCG  >  NZ_CP009273/2844771‑2845251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑