Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,725,0840GA41.5% 84.7 / 14.9 53L198L (TTG→TTA) xylFD‑xylose ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (27/4);  new base A (0/22);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 3.23e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACGATGCGGATATCGATTTTTATATTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGC  >  NZ_CP009273/3724840‑3725323
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               
aCGATGCGGATATCGATTTTTATATTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAAt                                                                                                                                                                                                                                            >  2:210566/1‑250 (MQ=255)
                      tatTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGc                                                                                                                                                                                                                       <  1:210848/249‑1 (MQ=255)
                        tttctttcGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGACAACGCATTGAAATTTATGGAAAACGCGCTAACCGCCaa                                                                                                                                                                                                                    >  2:297049/1‑250 (MQ=255)
                           ctttcGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCaataa                                                                                                                                                                                                                 >  2:381485/1‑250 (MQ=255)
                             ttcGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCaataat                                                                                                                                                                                                                >  2:323432/1‑249 (MQ=255)
                                   aaCGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACaaaa                                                                                                                                                                                                         >  1:369891/1‑250 (MQ=255)
                                        aaaaGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGAt                                                                                                                                                                                                    >  2:83347/1‑250 (MQ=255)
                                        aaaaGTCGGTGAACTGCAGACAAAAGCCCTGGTCAATATTGTTCCGCAAGGTAATTCCTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGAAAAATAAAAGTGTTAAACCCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAAGGGGTTGATGGCTGGTTACCGAAAAACGCATTAAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGAt                                                                                                                                                                                                    <  1:98597/250‑1 (MQ=255)
                                                        cAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCa                                                                                                                                                                                     >  1:321347/1‑249 (MQ=255)
                                                         aGGAAAAAGCCTTGTTCGATTTTGTCCCGCAAGGTATTTGTTGCCTGATGGGCGCCTCCCCGGTAAAAAACACCACCAAGCTGTCCCGCGCCGGAAAAATAAAAGTGTTAAACCCTTACTTTGATTCCGAAAAAATAAAAGTCTTTGGTGACCAATGGGTTGAGGGCTGTTTACCGAAAAACAAATTGAAATTTATGGAAACCGCGAAAGCCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCaaa                                                                                                                                                                                   <  2:141604/250‑1 (MQ=255)
                                                                    cTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGAAAAATGAAAGTTTTAAACCCTTACGTTGATTCCGAAAAAATTAAAGTCGTTGGTGACCAATGGTTTGATGGCTGGTTACCGGAAAACGCATTAAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCg                                                                                                                                                                        <  1:6677/250‑1 (MQ=255)
                                                                                   ccGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTCCCGCGCCGGACAAATAAAAGTGTTAAAACCTTACGTTGATTCCGAAAAAATTAAAGTCGTTGGTGACCAAGGGGTTGATGGCTGGTTACCGAAAAACGCATTAAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTc                                                                                                                                                         <  1:236318/250‑1 (MQ=255)
                                                                                          gTAATTCCTTCCGGGTGGGCGGCTCGCCGGAAAAAAACAACGCCAAGCTTTTCCGCGCCGGAAAAATGAAAGTGTAAAACCCTGACGTTGATTCCGAAAAAATAAAAGTCGTGGGTGACAAATGGTTTGATGGCTGGTTACCGAAAAACGCATTAAAAATTATGAAAACCGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCAtt                                                                                                                                                  <  2:524507/250‑1 (MQ=255)
                                                                                           tAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCAtt                                                                                                                                                  >  1:170317/1‑249 (MQ=255)
                                                                                             aTTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAg                                                                                                                                               >  2:327585/1‑250 (MQ=255)
                                                                                             aTTACTTCCTGATGGGCGGCCCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGAAAAAATAAAAGTTTTAAAACCTTACGTTGATTCCGAAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTCCCGGGAAACGCATTAAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTaa                                                                                                                                                <  1:248377/249‑1 (MQ=255)
                                                                                                           ggcggcTCGCCGTTAGAAAACAACGCCAAGCTGTTCCGCGCCGGACAAATAAAAGTGTTAAAACCTTACTTTGATTCGGAAAAAATAAAAGTCGTTGGTGACAAAGGGGTTGATGGCTGGTTACCGAAAAACGCATAGAAAATTAGGAAAACCGCGCAAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTAt                                                                                                                                 <  2:36253/250‑1 (MQ=255)
                                                                                                                    ccGGTAGATAACACCGCCAAGCTGTCCCGCGCCGAAAAAATGAAAGTGTTAAAACCTTACGTTGATTCCGAAAAAATAAAAGTCGTGGGTGACCAAGGGGTTGAGGGCTGGTTACCGAAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAg                                                                                                                        <  1:120058/250‑1 (MQ=255)
                                                                                                                      ggTAAATAAAAACGCCAAGCTGTCCCGCGCCGAAAAAAGAAAAGTGTTAAAACCTTACGTTAATTCCGAAAAAATAAAAGTCGTTGGTGACAAAGGGGTGGATGGCTGGTTACCGAAAAACGCATTAAAAATTAGGAAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTa                                                                                                                      <  2:197285/250‑1 (MQ=255)
                                                                                                                       gTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAg                                                                                                                     >  1:366171/1‑250 (MQ=255)
                                                                                                                       gTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAg                                                                                                                     >  1:523266/1‑250 (MQ=255)
                                                                                                                         aGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGc                                                                                                                    >  2:203439/1‑249 (MQ=255)
                                                                                                                          gATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAACCCTTACTTTGATTCCGAAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGAAAAACGCATTAAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCaa                                                                                                                  <  1:95724/250‑1 (MQ=255)
                                                                                                                          gATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGAAAAACGCATTAAAAATTATGGAAACCGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCaa                                                                                                                  <  1:83251/250‑1 (MQ=255)
                                                                                                                                     aaaGGTGTCCCGCGCAGGAAAAAGGAAAGTGTAAAAACCTTACGTTTATTCCGAAAAAATTAAAGTCGTGGGTGCCAAAGGGGGTGAGGGCTGTTTACCGAAAAACGAATAAAAAATTAGGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAg                                                                                                       <  2:266735/249‑1 (MQ=255)
                                                                                                                                              ccGCGCCGAAAAAATGAAAGTGTAAAAACCTTACGTTTATTCCGAAAAAATAAAAGTCGTTGGTGACCAAGGGTTTGATGGCTGTTTACCGAAAAACGCATTAAAAATTAGGAAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGAt                                                                                              <  1:223583/250‑1 (MQ=255)
                                                                                                                                                    cGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCg                                                                                          >  1:358419/1‑248 (MQ=255)
                                                                                                                                                       aaaaaTGAAAGTGTTAAACCCTTACGTTGATTCCGAAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTAAAAATTATGAAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGt                                                                                     <  1:5899/248‑1 (MQ=255)
                                                                                                                                                         aaaTGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTAt                                                                                   >  1:58511/1‑250 (MQ=255)
                                                                                                                                                                     tAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATtgc                                                                       >  1:193403/1‑250 (MQ=255)
                                                                                                                                                                               cGTTGATTCCGAAAAAATTAAAGTCGTTGGTGCCCAATGGGTTGATGGCTGGTTACCGAAAACCGCATTAAAAATTAGGGAAACCGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACg                                                             <  1:391785/250‑1 (MQ=255)
                                                                                                                                                                                gTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACg                                                             >  1:12167/1‑249 (MQ=255)
                                                                                                                                                                                  tGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCa                                                           >  1:8537/1‑249 (MQ=255)
                                                                                                                                                                                   gatgcggaaaaaaTAAAAGTCGTTGGTAACAAATGGGTGGATGGTGGTTTACAGAAAAACGAATGAAAAATTAGGGAAACCGCGAAAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCaa                                                          <  2:303006/241‑1 (MQ=255)
                                                                                                                                                                                    aTTCCGGAAAAATTAAAGTCGTTGGTGACAAATGGGTTGATGGCTGGTTACCGAAAAACGCATTAAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCTTTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAAc                                                        <  1:51108/250‑1 (MQ=255)
                                                                                                                                                                                      tCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTa                                                      >  1:102983/1‑250 (MQ=255)
                                                                                                                                                                                       ccGAAAAAATTAAAGTCTTTGGTAACAAATGGGTTGATGGCTGGTTACCGAAAAACGCATTAAAAATTATGAAAACCGCCCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTa                                                      <  2:523266/249‑1 (MQ=255)
                                                                                                                                                                                                ttAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGAAAAACGCATTAAAAATTATGAAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGta                                            <  1:344424/250‑1 (MQ=255)
                                                                                                                                                                                                   aaGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGtata                                          >  1:268306/1‑249 (MQ=255)
                                                                                                                                                                                                    aGTCGTGGGTGACAAAGGGGTTGATGGCGGTTTACAGAAAAACCCATTAAAAATTATGAAAACCGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATaaa                                        <  2:500861/250‑1 (MQ=255)
                                                                                                                                                                                                     gTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAAc                                       >  1:204413/1‑250 (MQ=255)
                                                                                                                                                                                                       cGTTGGTGACCAATGGGTGGATGGCTGTTCACAAAAAAACAAATAAAAAATTAGAGAAACCGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCt                                     <  2:526004/250‑1 (MQ=255)
                                                                                                                                                                                                        gtgggTGACCAAGGGTTTGAGGGCTGTTTACCGAAAAACGCATTAAAAATTATGAAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCt                                     <  1:423089/246‑1 (MQ=255)
                                                                                                                                                                                                             tGACAAATGGGTGGAGGGCTGGTTCCCAGAAAACGAATTAAAAATTAGGAAAACCGCGCAAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACg                               <  2:350235/250‑1 (MQ=255)
                                                                                                                                                                                                              gACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACgt                              >  1:311909/1‑250 (MQ=255)
                                                                                                                                                                                                                    tGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTgg                        >  1:337352/1‑250 (MQ=255)
                                                                                                                                                                                                                     gggTTGATGGCTGGTTCCCGAAAAACGCATTAAAAATTATGAAAACCGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGc                       <  1:299983/250‑1 (MQ=255)
                                                                                                                                                                                                                         tGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCaaa                    >  1:261130/1‑249 (MQ=255)
                                                                                                                                                                                                                          gATGGCTGGTTACCGGAAAACGCATTAAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAAt                   <  1:114142/249‑1 (MQ=255)
                                                                                                                                                                                                                             ggCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTg               >  1:129823/1‑250 (MQ=255)
                                                                                                                                                                                                                                  gTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCg            >  2:33593/1‑248 (MQ=255)
                                                                                                                                                                                                                                    tACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAg         >  2:171438/1‑249 (MQ=255)
                                                                                                                                                                                                                                          aaaaCGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGc  >  2:514375/1‑250 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               
ACGATGCGGATATCGATTTTTATATTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGC  >  NZ_CP009273/3724840‑3725323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑