Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,277,647	0	T	A	41.9%	49.8 / 26.0	43	intergenic (‑348/‑45)	acs/nrfA	acetate‑‑CoA ligase/ammonia‑forming nitrite reductase cytochrome c552 subunit
Reads supporting (aligned to +/- strand):  ref base T (21/4);  new base A (0/18);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.42e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTT‑ACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGCCCCGCAGCATCCCGATCAATATCTCTCCTGGAAAGCCACCTCGGAACAGTCAGAGCGTGTTGACGCCC  >  NZ_CP009273/4277419‑4277896                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                          aaCGGAGGGGTAATTTTTGAAGGTAAGCAAAAAAAGTTGATTAATTCTTTGAGGAACTTTCATTTATGCATGCTTTTAAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTTTTTGTT‑ACACATAGGGGCGAGAAATGTCATGACAGTGTAGGTGCGTTTACTGTCGTAAAAAACAAAAAAAACCGCCATTGCAACAAt                                                                                                                                                                                                                                      <  2:527624/250‑1 (MQ=255)      cAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTT‑ACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGgcg                                                                                                                                                                                                                                  >  1:186396/1‑249 (MQ=255)               ttttGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTT‑ACACATA‑GGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGGGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGAt                                                                                                                                                                                                                        >  1:129015/1‑249 (MQ=255)                    aaGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTT‑ACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGc                                                                                                                                                                                                                   >  2:73037/1‑250 (MQ=255)                      ggTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTT‑ACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGccc                                                                                                                                                                                                                 >  1:425280/1‑250 (MQ=255)                                        aTTAATTTTTGGAGGAACATGCATTTATGCATGCTGTTAAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTT‑ACACATAGGGGCGAGCAATGTAATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATaaaa                                                                                                                                                                                                <  1:305847/249‑1 (MQ=255)                                         ttAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTT‑ACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATaaaaa                                                                                                                                                                                               >  1:201766/1‑249 (MQ=255)                                            ttttttggagaAACATGCTTTTAGGCATTTTTTAAAAAAAGAGAAAGATACTAACAACCTAAAAAGTGTTTTTTTACATCCATTAAAAATTAAAAAAAAACAACATCCACGGTGTGTTTTTTTTTA‑ACA‑AAAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTAAAAAACAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAac                                                                                                                                                                                          <  2:129015/243‑1 (MQ=255)                                            aTTCTTTGAGGAACATGCAGTTATGTATGCTGTTGAAAAAGAGGAAGATACTGACTAACTATAAAGTGGTTTTTTACATGCACTGACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTT‑ACACATGGGGGCGAGAAATGTCATGACAGTTTGGGTGGGGTTACTGTCGTGAAAAAAAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATaaa                                                                                                                                                                                           <  2:426896/250‑1 (MQ=255)                                              tCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTT‑ACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAacg                                                                                                                                                                                         >  1:90115/1‑250 (MQ=255)                                               ttttGAGAAACATGCATTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTAAAATTGATAAAAGACAACATTCACAGTGTGGTTATTTGTT‑ACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAacg                                                                                                                                                                                         <  2:64630/248‑1 (MQ=255)                                                         atggaggtaaggcatgtTGTTAAAAAAGAGAAAGATACTGATAAACTAAAAAGGGTTTTTTTAAATCCATTAAAATTTAATAAAAAAAAACATCAAAGGTGTGTTTATTTTTA‑AAAAAGGGGGGCAACAATTGTAATGAAAGTGTAGGGGCGTTTACTGTCGAAAAAAAAAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATc                                                                                                                                                                              <  2:264917/239‑1 (MQ=255)                                                          aTGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTT‑ACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCt                                                                                                                                                                             >  2:193481/1‑250 (MQ=255)                                                               tttATGCATCCTGTTAAAAAAGAGGAAGATACTGACTAACTCAAAAGTGGTATTTTACATGCATTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTTTT‑ACACATGGGGGCGAGCAATGTCATGACAGTGTAGGTGCGTTTACTGTCGTAAAAAACAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGc                                                                                                                                                                        <  1:97376/249‑1 (MQ=255)                                                               gTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTT‑ACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGc                                                                                                                                                                        >  2:24483/1‑250 (MQ=255)                                                                   tgcatgcTTTTAAAAAAGAGGAAGATACGGACTAACTAAAAAGTGGTTTTTTACATGCATTTAAAATTGATAAAAAAAAACTTTAATGGTGTGGTTTTTTTTT‑CCAAAGGGGGGCGAAAAATTCAATGACGGTGTGGGTGCGTTTACGGTGGAAAAAAAAAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCATATCTTCAGCTTAt                                                                                                                                                                    <  2:127154/250‑1 (MQ=255)                                                                     catgcTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTT‑ACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTAtt                                                                                                                                                                   >  1:403492/1‑249 (MQ=255)                                                                          tGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTT‑ACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTcc                                                                                                                                                             >  2:7171/1‑250 (MQ=255)                                                                            ttaaaaaaGAGAAAAATCCTGACTAACTCAAAAGTGGTATTTTACATGCATTTAAAATTGATAAAAGAAAACATTCACAGTGTGGTTTTTTGTT‑ACAAATAGGGGCGAGAAATGTCATGACAGTGTAGGTGCGTTTACTGTCGTAAAAAAAAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCtt                                                                                                                                                           <  1:41618/247‑1 (MQ=255)                                                                                 aaaGAGAAAGATACTGATAAACTAAAAAGTGGTATTTAACATGCATTTAAAATGAATAAAAGAAAACTTTCACAGGGTGTTTATTTTTT‑ACAAAAAGGGGCGAGAAATGTCAGGACAGTGTGGGTGGGGTTAGTGTGGGAAAAAAAAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTtttttc                                                                                                                                                      <  2:13426/250‑1 (MQ=255)                                                                                          gATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTT‑ACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACt                                                                                                                                             >  2:252702/1‑250 (MQ=255)                                                                                           aaactaaaaaaCTCAAAGGTGTTTTTTTACATGCATTAAAATTTAATAAAAAAAAACTTCCACTGTGTGTTTTTTTTTA‑ACAAAGGGGGGCAAGAAATGTAAGGACAGTGTGGGTGGGTTTACTGTCGAAAAAAAAAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACtt                                                                                                                                            <  2:425280/241‑1 (MQ=255)                                                                                           aTACTGACTAACTCAAAAGTGGTATTTTACTTGCCCTTAAAATTAATAAAAGAAAACATCCACGGTTTGTTTTTTTGTA‑ACACATGGGGGCGAGCAATGTAATGAAGGTGTAGGTGCGTTTACTGTCGTAAAAAAAAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTCCACtt                                                                                                                                            <  1:73037/250‑1 (MQ=255)                                                                                                 acaaacttaaaaGTGGTTTTTTAAATGCATTTAAAATTAATAAAAGAAAACATTCAAGGTGTGGTTTTTTGTT‑ACAAAAGGGGGGGAGAAATGTCAGGAAAGTGTGGGGGGGGTTACTGTGGTAAAAAAAAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGt                                                                                                                                        <  1:203960/239‑1 (MQ=255)                                                                                                 aCTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTT‑ACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGtt                                                                                                                                       >  2:458538/1‑249 (MQ=255)                                                                                                      ctctAAAGTGTTTTTTTACATCCACTTACAATTGATAAAAGACAACATTCACAGTGTGTTTATTTTTT‑ACACATGGGGGAGAGAATTGTCATGACAGTGTGGGTGCGTTTACTGTCGTAAAAAACAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCt                                                                                                                                 <  1:145582/250‑1 (MQ=255)                                                                                                              tGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTT‑ACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAAc                                                                                                                         >  1:63720/1‑250 (MQ=255)                                                                                                                     ttACATGCATTTAAAATTGATTAAAGACAACATTCACAGTGTGTTTATTTGTT‑ACAAATGGGGGCGAGAAATGTCATGACAGTGTAGGTGGGGTTACTGTCGGAAAAAAAAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGgctgc                                                                                                                   <  1:472706/249‑1 (MQ=255)                                                                                                                                 aCAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTT‑ACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCt                                                                                                      >  2:32384/1‑250 (MQ=255)                                                                                                                                            aaGACAACATTCAGAGTGT‑GTTTTTTGTTCACACAGGGGGGCGAAAAATGTCATGACGGTGTAGGGGCGTTTACTGTCGAAAAAAAAAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGa                                                                                           <  2:121046/250‑1 (MQ=255)                                                                                                                                                   catccaggttgtgTTTTTTTGTT‑ACAAATGGGGGCGAGAATTGTCATGACAGTGTAGGTGGGTTTCTTGTCGAAAAAAACAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCgaag                                                                                    <  1:305478/241‑1 (MQ=255)                                                                                                                                                    aTTCACAGTGTGGTTATTTGTT‑ACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCgaag                                                                                    >  2:60389/1‑249 (MQ=255)                                                                                                                                                              tgGTTTTTTGTT‑ACACATAGGGGCGAGAAATGTAATGACAGTGTGGGTGCGGTTACTGTCATGAAAAACAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCt                                                                          <  2:370588/249‑1 (MQ=255)                                                                                                                                                                gTTATTTGTT‑ACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTg                                                                       >  2:500715/1‑250 (MQ=255)                                                                                                                                                                    tttGTT‑ACACATAGGGGCGAGCAATGTCCTGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGcccc                                                                   >  2:260046/1‑250 (MQ=255)                                                                                                                                                                                   gggCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGCCCCGCAGCATCCCGAt                                                      >  2:486362/1‑249 (MQ=255)                                                                                                                                                                                           aatgcaaagACAGTGTGGGGGCGGTTACTGTGGAAAAAAAAAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGCCCCGCAGCATCCCGATCAATAtctc                                             <  2:87423/242‑1 (MQ=255)                                                                                                                                                                                                cATGACATTGTAGGGGGGGTTACTGTCGTAAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAAGTTAATAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGCCCCGCAGCATCCCGATCAATATCTCTCCt                                         <  1:353520/249‑1 (MQ=255)                                                                                                                                                                                                 aTGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGCCCCGCAGCATCCCGATCAATATCTCTCCTg                                        >  2:6644/1‑249 (MQ=255)                                                                                                                                                                                                                cGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGCCCCGCAGCATCCCGATCAATATCTCTCCTGGAAAGCCACCTCGGaa                        >  2:93084/1‑250 (MQ=255)                                                                                                                                                                                                                    tACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGCCCCGCAGCATCCCGATCAATATCTCTCCTGGAAAGCCACCTCGGAACAGt                    >  2:466858/1‑250 (MQ=255)                                                                                                                                                                                                                      ggttcggaaaaacCAAAAAAAACCGCCATTGCAACAAGGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGTCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGCCCCGCAGCATCCCGATCAATATCTCTCCTGGAAAGCCACCTCGGAACAGTCa                  <  2:405340/242‑1 (MQ=255)                                                                                                                                                                                                                                aaaCAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGCCCCGCAGCATCCCGATCAATATCTCTCCTGGAAAGCCACCTCGGAACAGTCAGAGCGTGTTg        <  1:7171/250‑1 (MQ=255)                                                                                                                                                                                                                                      tAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTCCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGCCCCGCAGCATCCCGATCAATATCTCTCCTGGAAAGCCACCTCGGAACAGTCAGAGCGTGTTGACGccc  >  1:320637/1‑250 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                          AACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTT‑ACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGCCCCGCAGCATCCCGATCAATATCTCTCCTGGAAAGCCACCTCGGAACAGTCAGAGCGTGTTGACGCCC  >  NZ_CP009273/4277419‑4277896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑