Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	117,994	0	A	T	40.0%	81.2 / 24.8	55	L15L (CTT→CTA)	aroP	aromatic amino acid transporter AroP
Reads supporting (aligned to +/- strand):  ref base A (8/25);  new base T (22/0);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 4.79e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCAGCGAATGCACCCGCTTTATGCATGGTTGAAGATGAGTTGCTTAAAAAGAAACCG  >  NZ_CP009273/117753‑118242                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                          cagAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTTATGCagcg                                                                                                                                                                                                                                                   >  2:362822/1‑250 (MQ=255)        gAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAgc                                                                                                                                                                                                                                            >  2:444639/1‑250 (MQ=255)        gAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGGTTTTTAGGCcgccccttcgc                                                                                                                                                                                                                                            >  2:484758/1‑242 (MQ=255)          aaCCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAgctg                                                                                                                                                                                                                                          <  1:302429/250‑1 (MQ=255)                        ccccAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTTTATGCGCGCTTTTGCTGCTCCCCggtccggt                                                                                                                                                                                                                            >  2:517513/1‑243 (MQ=255)                              tATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTTAGGCCCCGCTTTTGCTGCCTGCCGTGCTGGTGagcct                                                                                                                                                                                                                      >  1:53922/1‑246 (MQ=255)                              tATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCtt                                                                                                                                                                                                                      <  1:177834/250‑1 (MQ=255)                               aTTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTc                                                                                                                                                                                                                     >  1:326435/1‑250 (MQ=255)                                         aaaGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTTAGGCCGCGCTTTTGCTGCTCGCCGTGCTGTTGGCCTTCCCTCA‑TGaa                                                                                                                                                                                                           >  1:361088/1‑249 (MQ=255)                                         aaaGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTTAGGCCCCGCCTTTGCTGCTCGCCCCGCCGTTTGCCCTCCCTCC‑TGaaa                                                                                                                                                                                                          >  2:268644/1‑250 (MQ=255)                                                 tAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTTAGGCCGCGCTTTAGCTGCTCGCCGCGCTGTTGTCCTTCCATCA‑TGAAAACTCGTGc                                                                                                                                                                                                  >  1:34618/1‑250 (MQ=255)                                                   aaGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAAGCCGCGCCTTTGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCgg                                                                                                                                                                                                >  1:421048/1‑250 (MQ=255)                                                    aGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAGCCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCggt                                                                                                                                                                                               <  1:332934/250‑1 (MQ=255)                                                        gCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTTATGCCCCGCTTTAGCTGCTCGCCCTGCTGTTTACCTTCCCTCA‑TGAAAACTCGTGCggtgggt                                                                                                                                                                                           >  1:90480/1‑248 (MQ=255)                                                         ccTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGGTGgttg                                                                                                                                                                                          <  2:215334/250‑1 (MQ=255)                                                             cGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGGTGGTTGtttt                                                                                                                                                                                      <  1:505394/250‑1 (MQ=255)                                                                       ttcGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTTAAGCCGCGCCTTTGCTGCTCGCCGTGCTGTTGTCCCTCCACCT‑TGAAACCTTCTGCGGGGGGTGttttttttgtttac                                                                                                                                                                            >  2:98953/1‑243 (MQ=255)                                                                         cGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTAc                                                                                                                                                                            <  2:10048/248‑1 (MQ=255)                                                                          gACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCa                                                                                                                                                                         <  1:58210/250‑1 (MQ=255)                                                                             caccaTTTCACCCAGCCGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAAGCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTGCGATCA‑TGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTg                                                                                                                                                                      <  2:114636/250‑1 (MQ=255)                                                                                       cccAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCCTCA‑TGAAACCTCGTGCGGGGGTTGTTTTTTTGATCTACGCAGTGATGCgtgtg                                                                                                                                                             >  1:100208/1‑249 (MQ=255)                                                                                         cAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATGCGTGTGTaa                                                                                                                                                          <  2:367628/250‑1 (MQ=255)                                                                                                      aTCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTcc                                                                                                                                              <  1:476239/249‑1 (MQ=255)                                                                                                       tCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCGGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGt                                                                                                                                            <  2:42213/250‑1 (MQ=255)                                                                                                              ggCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTtgttgt                                                                                                                                     <  1:245113/250‑1 (MQ=255)                                                                                                               gCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTTAGGCCGCGCCTTTGCTGCCCGCCGGGCTGTTGGGCTTCCCTCA‑TGTAACCTCGTGCGGTGGGTGTTTTTTTTTTTTTTGCCGTGATTGGTGTGTAAGTTTGTAATTTtgtttgttttt                                                                                                                                    >  2:488423/1‑247 (MQ=255)                                                                                                                cGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTTTGGGCGCGCTTTAGCTGCTCGCCCTGCGGTTGAGCCTCCATCA‑TGAAACCCCCTGCGGGGGGTGTTTTTTTTTTTTTCACCGTGGTGCGGGGGTCAGTTTGCCACTCCTTTTGTTTTAt                                                                                                                                   >  2:318895/1‑250 (MQ=255)                                                                                                                       aCCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTTAGGCCGCGCTTTAGCTGCTCGCCGGGCTGTTGACCTTCCCTCA‑TGAAACCTCGTGCGGTGGGTGTTTTTTTGTTCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTatttatttt                                                                                                                            >  1:186592/1‑249 (MQ=255)                                                                                                                             aaTGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGTTGGTTGTTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACa                                                                                                                      <  2:57700/250‑1 (MQ=255)                                                                                                                              aTGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACAt                                                                                                                     <  1:171555/250‑1 (MQ=255)                                                                                                                                   gTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTTATGCCGCCCCTTTGCTGCTCGCCGGGCTGTTGTCCTTCCCTCA‑TTAAACCTCCTGCGGGGGGTGTTTTTTTTTTTTTCTCCGTGGTGCGTGTGGTAGTTTTGAATTTCTTTTTTTTTTTTTATTTTTTTTCTTCa                                                                                                                   >  2:4882/1‑247 (MQ=255)                                                                                                                                    tAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGCGGGTTTTTAAGG‑CGCGCCTTAACTGGCCGCCCGGCTGTTTGGCTTTCCTCATTGAAACC‑CGTGCGGGGGGGGTTTTTTTTTTTTTTGCAGGGCTGCGGGGGTGAGGTTGGGATTTCGTTTGTTGTATTAATttttttttatacaaa                                                                                                                >  2:277702/1‑240 (MQ=255)                                                                                                                                    tAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTTAAGGCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCCTCA‑TGAAAACTCGTGCGGGGGTTGGTTTTTTGTTCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAaag                                                                                                                >  1:146993/1‑249 (MQ=255)                                                                                                                                       cccAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGt                                                                                                            <  1:56203/250‑1 (MQ=255)                                                                                                                                         cAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTTAGGCCGCGCCTTTGCTGCTCGCCGGGCTGTTGTCCTTCCATCA‑TGAAACCTCGTGCGGTGGTTGGTTTTTTTGTTTACGCAGTGGTGGGTGTGTAGGTTTTCAATTTCGTTTGTTGTATTTATTTTTTTTCTTCAAAGAAGttt                                                                                                          >  2:437116/1‑250 (MQ=255)                                                                                                                                         cAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGttt                                                                                                          <  2:326435/250‑1 (MQ=255)                                                                                                                                             aTAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAAt                                                                                                      <  1:68744/250‑1 (MQ=255)                                                                                                                                                aTCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTTAAGCCGCGCTTTTGCTGCTCGCCGTGCTGTTTACCTTCCATCA‑TGAAAACTCGTGCGGGGGGTGTTTTTTTTATCTACGCCGGGATGCGGGTGTAAGTTTTCAATTCCGTTTGTTTTATTAATTTGTTTTCATCAAAGAAGTTTTAATTGt                                                                                                   >  1:360587/1‑250 (MQ=255)                                                                                                                                                     tGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGGTTTTTAAGCCGCGCCTTTGCTGGCCGCCGGGCTGGTGACCTTCCCTTC‑TGAAACCTCGTGGGGGGGGTGGTTTTTTTTTTTTCGCCGGGGTGGGTGTGGAAGGTTGGAATTGCGTTTGTTTTTTTTATTTTTTTTTATCAAAGAAGTTTGAATTGtttct                                                                                               >  2:139838/1‑246 (MQ=255)                                                                                                                                                       gCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACaa                                                                                              <  1:22403/248‑1 (MQ=255)                                                                                                                                                        cccTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGGTGGGTGTTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACaaaa                                                                                            >  1:148102/1‑249 (MQ=255)                                                                                                                                                          cTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGGTTTTTAAGCCGCGCTTTTGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGGGGGGGGTGGTTTTTTTATTTACGCAGGGATGCGTGTGGAAGATTGCAATTCCGTTTTTTTTTTTAATTTGTTTTCATCAAAGAAGTTTTAATTGTTTCaaaaaa                                                                                          >  2:12467/1‑248 (MQ=255)                                                                                                                                                             cGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTTAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGGGGGGTGTTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACtt                                                                                      >  1:316791/1‑250 (MQ=255)                                                                                                                                                              ggACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTTAGGCCGCGCTTTTGCTGCTCGCCGTGCTGTTTGCCTTCCCTCA‑TGAAACCTCGCGCGGGGGGTGTTTTTTTTTTCTTCGCAGTGATGCGCGTGTAAGTTTGCAATTCCGTTTGTTTTTTTAATTTTTTTACATCCAAAAAGTTTTAATTTTTATAAAAAAACTTc                                                                                     >  2:18695/1‑250 (MQ=255)                                                                                                                                                              ggACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTc                                                                                     <  1:444639/250‑1 (MQ=255)                                                                                                                                                                    tATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAg                                                                               <  1:322608/250‑1 (MQ=255)                                                                                                                                                                                gcTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGaataat                                                                   <  1:518258/250‑1 (MQ=255)                                                                                                                                                                                    cccAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTTAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGGGGGTTGTTTTTTTTATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTTCGTTTTTTGTATTAATTTGTTTTCATCAAAGAAGTTTTAATTGTTTCAAAAAGACTTCCGTCAGATCAAGAATAATGGTa                                                               >  1:100066/1‑250 (MQ=255)                                                                                                                                                                                                 cGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTTAAGCCGCGCTTTTGCTGCCCGCCCTGCTGTTGACCTTCCATCA‑TGAAACCCCGTGGGGGGGGTGTTTTTTTTTTCTTCGCAGGGATGCGTGGGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTTTTTTCATCAAAGAAGGTTGAATTTTTACAAAAAAACTTTCGGCAGATCAAAAAAAATGGTATGCGGCAGCGa                                                    >  2:149178/1‑248 (MQ=255)                                                                                                                                                                                                                   ccAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCAGCGAATGCACCCGCTTTATGCATg                                <  1:337577/250‑1 (MQ=255)                                                                                                                                                                                                                    cAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCAGCGAATGCACCCGCTTTATGCATgg                               <  2:381008/250‑1 (MQ=255)                                                                                                                                                                                                                         cgATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCAGCGAATGCACCCGCTTTATGCATGGTTGaa                          <  2:188677/250‑1 (MQ=255)                                                                                                                                                                                                                                            ggTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCAGCGAATGCACCCGCTTTATGCATGGTTGAAGATGAGTTGCTTAAAAAg        <  1:4882/250‑1 (MQ=255)                                                                                                                                                                                                                                               ttttAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAACAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCAGCGAATGCACCCGCTTTATGCATGGTTGAAGATGAGTTGCTTAAAAAGaaa     >  2:419883/1‑250 (MQ=255)                                                                                                                                                                                                                                                  tAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCAGCGAATGCACCCGCTTTATGCATGGTTGAAGATGAGTTGCTTAAAAAGAAACCg  >  1:257743/1‑250 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                          CAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAATATGGC‑GGTTTTTAAGGCCGCGCTTTAGCTGCTCGCCGTGCTGTTGACCTTCCATCA‑TGAAACCTCGTGCGGTGGTTGTTTTTTTGATCTACGCAGTGATGCGTGTGTAAGTTTGCAATTCCGTTTGTTGTATTAATTTGTTTACATCAAAGAAGTTTGAATTGTTACAAAAAGACTTCCGTCAGATCAAGAATAATGGTATGCGGCAGCGAATGCACCCGCTTTATGCATGGTTGAAGATGAGTTGCTTAAAAAGAAACCG  >  NZ_CP009273/117753‑118242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑