Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273748,5810TA43.1% 69.1 / 23.6 51intergenic (‑330/+60)ybgD/gltAfimbrial protein/citrate synthase
Reads supporting (aligned to +/- strand):  ref base T (26/3);  new base A (0/22);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.47e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AAATATTGTTAGTTTTATTAAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACC  >  NZ_CP009273/748338‑748826
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     
aaaTATTGTTAGTTTTATTAAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAAt                                                                                                                                                                                                                                                 >  2:268778/1‑250 (MQ=255)
aaaTATTGTTAGTTTTATTAAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAAt                                                                                                                                                                                                                                                 >  1:30426/1‑250 (MQ=255)
               tattaAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATg                                                                                                                                                                                                                                   >  1:377721/1‑249 (MQ=255)
                        cAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGt                                                                                                                                                                                                                         >  1:39436/1‑250 (MQ=255)
                        aaaaaCTAAATTTTTGGTATCATAATTTTTTTGTATGATAAATAAAATATAGGGGGGTATAGATAGACGTTTTTTTAATGGGGTTAAAAATGCGACTACCATGAATTTTTTAATTAAAAGTTTGGGGTTGCTAATATTTTGAGTTTTTTTTTTTTTTTAAAATTTTTATATAGTTTTTTTAATGTTTTTTTTTTTTAAATTTTTTTGTGTTTAGGAATAAAAAAATCACCCCGCCATATGAACGGCGGGt                                                                                                                                                                                                                         <  2:285249/249‑1 (MQ=255)
                         aaaaCTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGt                                                                                                                                                                                                                         >  1:88637/1‑249 (MQ=255)
                          aaaCTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACACTTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGTTTa                                                                                                                                                                                                                       >  2:361459/1‑250 (MQ=255)
                             cTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTaaaa                                                                                                                                                                                                                    >  1:315525/1‑250 (MQ=255)
                                 attattGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATAtt                                                                                                                                                                                                                >  2:200498/1‑250 (MQ=255)
                                            aattaaTTTTTTTTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCTTTTTCATAGGTTTAAAAATGCGACTACCATGAAGTTTTTAATTAAAAGTATTGGGTTGCTGATATTTTGGGCTTTTTTTTTTTTTTTAAATATCTATATAGGTCTGTTAAGGGTTTTTATTTTTACATTTTTTTGTTTTTAGAAATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAg                                                                                                                                                                                                      <  2:286310/245‑1 (MQ=255)
                                              tGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCaa                                                                                                                                                                                                   >  1:374297/1‑250 (MQ=255)
                                              tGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTAAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTAAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCaa                                                                                                                                                                                                   <  1:200498/250‑1 (MQ=255)
                                                attttGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACTTCATTTTCATAGGGTTATAAATGCGACTACCATGAATTTTTAAATTAAAAGTATTGGGTTGCTGATAATTTGAGTTGTTCTATTTTTTTAAAATATCTATATAGGTCTGTTAAGGAATTTTATTTTTACAATTTTTTGTGTTTAGGAATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAAt                                                                                                                                                                                                  <  1:102093/247‑1 (MQ=255)
                                                    tgttgtATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAAc                                                                                                                                                                                              >  1:456433/1‑249 (MQ=255)
                                                       tgtATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTTAAATATTTACAACTTAGCAATCAACCAt                                                                                                                                                                                           >  2:220322/1‑249 (MQ=255)
                                                         tATGATAAAAAAAATATAGGGGGGTATAGATAGACGTATTTTTCATGGGTTTATAAAGGCAACTACCATGAAGTTTTTAATTGAAAGTTTTGGTTTCCTAATATTTTGAGCTGTTTTTTTTTTTTTAAATATTTATATGGGTCTTTTAATGGTTTTTATTTTTACAATTTTTTGTGTTTAGGAATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTa                                                                                                                                                                                         <  1:209028/249‑1 (MQ=255)
                                                         ataagaaaaaaaaaTA‑AGGGGGGTAAAAAAAGACGTATTTTTCATAGGTTTAAAAATGCGACTACAATGAATTTTTTAATTAAAAGTATGGGGTTGTTGATATTTTGATTTGTTTTTTTTTTTTAAAATATTTATATAGGTCTGTTAATGATTTTTATTTTTAAAATTTTTTTTTTTTAGGAATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTaa                                                                                                                                                                                        <  2:198087/239‑1 (MQ=255)
                                                           tgataaAAAAAATATGGGGGGGTATAGATAGACTTATTTTTCATGGGGTTATAAATGCGACTACAATGAATTTTTTAATTAAAAGTATTGGTTTGCTAATATTTTGAGTTTTTTTTTTTTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTTTTTTTACAATTTTTTGTTTTTAGACATAAAAAAACCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAAc                                                                                                                                                                                       <  2:495254/249‑1 (MQ=255)
                                                                  tAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTATAAATTAGCAATCAACCATTAAAGCTTGata                                                                                                                                                                               >  2:464883/1‑250 (MQ=255)
                                                                   aaaaTATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATTTTTTCCAATTTTCAATCAACCCATAAAGCTTGgtat                                                                                                                                                                              >  2:362424/1‑250 (MQ=255)
                                                                       tataGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTAAAAGTATTGGGTTGCTGATAATTGGAGCTTTTCTATTTTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCt                                                                                                                                                                          <  1:387992/250‑1 (MQ=255)
                                                                          ggggggtTTTAGATGGACTTATTTTTAATAGGTTTATAAATGCGACTCCCATGAATTTTTTAATTAAAATTTTGGGTTTTTTAATATTTTGAGTTGTTTTTTTTTTTTAAAATATTTATATAGTTTTTTAAATGGTTTTTATTTTTAAATTTTTTTGTTTTAAGAAATAAAAAAAACAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCtttt                                                                                                                                                                       <  2:337468/249‑1 (MQ=255)
                                                                                 tataGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTcg                                                                                                                                                                >  1:185750/1‑250 (MQ=255)
                                                                                   tagatagaCGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTATAACTTAGCAAACAACCATTAACGCTTGATATCGCTTTTAAAGTcgcg                                                                                                                                                              >  2:178585/1‑250 (MQ=255)
                                                                                          aCGTCATTTTCATAGGTTTAAAAAGGCGACTACAATGAAGTTTTTAATTGAAAGTATGGGGTTTCTGATAATTTGAGCTTTTTTATTCTTTTTAAATATCTATATAGGTTTGTTAAGGGATTTTATTTTAACAATTTTTTGTGTTTAGGAATATAAAAAGAAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCa                                                                                                                                                       <  1:240120/250‑1 (MQ=255)
                                                                                                        gggTTATAAATGCGACTACCATAAATTTTTTAATTGAAAGTTTTGGTTTGTTTTTATTTTGAGTTTTTTTTTTTTTTTTAAATATATATATAGGTTTGTTAATGGTTTTTTTTTTAAAATTTTTTTGTTTTTAGGAATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACa                                                                                                                                          <  2:88948/249‑1 (MQ=255)
                                                                                                             ataAATGCTACTACCATGAAGTTTTTAATTGAAATTTTTGGGTTGCTAATAATTTGAGCTGTTCTATTCTTTTAAAATATCTATATAGGTCTTTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGAATAAAAAAATAAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGa                                                                                                                                     <  1:186136/249‑1 (MQ=255)
                                                                                                               aaaTGCGATTACCATGATTTTTTTAATTAAAAGTTTTGGGTTGCTAAAAATTTGAGCTTTTTTTTTTTTTTTAAATATTTATAGAGGTTTGTTAATGTTTTTTTTTTTTACAATTTTTTTTTTTAAGGAAAAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGAcgc                                                                                                                                  <  2:484726/250‑1 (MQ=255)
                                                                                                                  tGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGGTTTTCATATCCTGTATACAGCTGACGCGGa                                                                                                                               >  1:75484/1‑250 (MQ=255)
                                                                                                                     gACTACAATGAATTTTTTAATTGAAAGTATTGGGTTGCTGATATTTTGAGCTGTTTTTTTTTTTTTAAATATCTATATAGGTCTGTAAATGGATTTTATTTTTACATTTTTTTGTTTTTAGGCAAAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACgg                                                                                                                            <  1:13962/250‑1 (MQ=255)
                                                                                                                          ccATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAAt                                                                                                                       >  1:226141/1‑250 (MQ=255)
                                                                                                                                atttttaAATTGAAAGTATTGGTTTGTTGATAATTTGAGCTTTTCTATTTTTTTTAAATATCTATATAGGTTTTTTAATGGATTTTATTTTTACATTTTTTTGTTTTAAGGCATAAAAAAACCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAAATTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCAt                                                                                                                 <  1:95231/248‑1 (MQ=255)
                                                                                                                                        aTTAAAAGTTTTGTTTTGCTAATATTTTGTTTTTTTTTTTTTTTTTTAAATATCTATAGAGGTCTTTTAATGTTTTTTTTTTTTACATTTTTTTTTGTTTAGGCATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCa                                                                                                          <  2:21925/249‑1 (MQ=255)
                                                                                                                                            aaaGTATTGGTTTGCTAATATTTTGAGCTTTTTTATTTTTTTTAAATATCTATATAGGTCTGTTAATGTATTTTATTTTTAAAATTTTTTGTTTTTAGGAAAAAAAAAACCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACtgt                                                                                                      <  2:176549/249‑1 (MQ=255)
                                                                                                                                              aGTTTTGGGTTGCTGATAATTTGAGCTGTTTTATTTTTTTTAAATATTTATATAGGTCTGTTAATGGATTTTATTTTTACATTTTTTTGTTTTTAGGAATAAAAAAACCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCa                                                                                                   <  1:68096/250‑1 (MQ=255)
                                                                                                                                                         gCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAg                                                                                        >  2:521618/1‑250 (MQ=255)
                                                                                                                                                              tAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGc                                                                                   >  1:502414/1‑250 (MQ=255)
                                                                                                                                                                 tttGATTTGTTTTATTCTTTTTAAATATTTATATAGTTTTGTTAATGGTTTTTATTTTAACAATTTTTTGTGTTTAGGCATAAAAAAACCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGAt                                                                                <  1:89585/250‑1 (MQ=255)
                                                                                                                                                                              aTTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATACTGTATACAGCTGACTCGGACGGGCAATCCTCATACCGTCACTGTGCATTTCCCTCCAGTGGGCGATCCAGCCAACGGTa                                                                   >  1:152216/1‑250 (MQ=255)
                                                                                                                                                                                tCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACg                                                                 >  1:347638/1‑250 (MQ=255)
                                                                                                                                                                                       aaaTATATATATATTTTTTTTAATTGATTTTTTTTTTACATTTTTTTGTGTTTAGAAATAAAAAAAACACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCAt                                                           <  2:88637/249‑1 (MQ=255)
                                                                                                                                                                                         atatCTATATAGGCCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTTAAATATTTTAAACTTAGCAATCAACCATTAAAGCTTGATATCGCTTTTAAAGTAGCGGTTTTCATATCCTGTATACAGCTGACGCGGAAGGGCAAACTTCATAACGTCAATGTGCATTTCGCTCCAGTGGGGGGTCCAGCCAACGGTAAGATCCATTGc                                                        >  2:236241/1‑250 (MQ=255)
                                                                                                                                                                                          tttttatataGTTTTTTTAATGGATTTTTTTTTTACATTTTTTTGTGTTAAGGCAAAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCg                                                       <  2:61026/246‑1 (MQ=255)
                                                                                                                                                                                           atCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCCGCCAACGGTACGTGCCATTGCg                                                       >  1:307686/1‑249 (MQ=255)
                                                                                                                                                                                                  taGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAAATTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCCGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGAc                                               >  2:185161/1‑250 (MQ=255)
                                                                                                                                                                                                   aGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTTCAACTTAACAATCAACCATTAACGCCTGGTATCGCTTTTAAAGTCGCGTTTTTCATATCCTTTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTTGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACg                                              >  2:249986/1‑250 (MQ=255)
                                                                                                                                                                                                                        tttaACAATTTTTTTTTTTAAGGCTTATAAAAACAACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAAt                          <  1:394473/249‑1 (MQ=255)
                                                                                                                                                                                                                          ttACAATTTTTTGTGTTTAGGAAAATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATAc                        <  1:58919/249‑1 (MQ=255)
                                                                                                                                                                                                                            aCATTTTTTTGTGTTTAGGAATAAAAAAACCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATAccc                      <  1:43548/249‑1 (MQ=255)
                                                                                                                                                                                                                                  ttttGTGTTTAGAAAAAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCt                <  2:72892/249‑1 (MQ=255)
                                                                                                                                                                                                                                         tttAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGgat        >  2:253159/1‑250 (MQ=255)
                                                                                                                                                                                                                                          taagcaaaataAAATACAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGgat        <  2:483983/241‑1 (MQ=255)
                                                                                                                                                                                                                                                atatAAAAATCAACCCGCCATATGAACGGCGGGTTAAAAAATTTTCAAATTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAATCGCGTTTTTTATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTTCATTTTGCTCCAGTGGGCGAGCCAGCCAACGGTACGTGCCCTTGCGAAAATAACGGGGAACAAGGAAGACGGAATACCCCTCCGTTTCAGGATGATAcc  >  2:140572/1‑249 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     
AAATATTGTTAGTTTTATTAAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACC  >  NZ_CP009273/748338‑748826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑