Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,377,979	0	G	A	41.7%	82.2 / 29.3	60	F80F (TTC→TTT)	ycjW	LacI family DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (27/8);  new base A (0/25);  total (27/33)
Fisher's exact test for biased strand distribution p-value = 4.48e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGAATAAACATGGGCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGC‑AAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCAATATCATAAA  >  NZ_CP009273/1377733‑1378205                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                    aGAATAAACATGGGCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGC‑AAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGaa                                                                                                                                                                                                                                   >  1:267417/1‑249 (MQ=255)   aaTAAACATGGGCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGC‑AAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGaaaaa                                                                                                                                                                                                                                <  1:238073/250‑1 (MQ=255)    aTAAACATGGGCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGC‑AAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑c                                                                                                                                                                                                                              <  1:398456/250‑1 (MQ=255)     tAAACATGGGCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGC‑AAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑Cg                                                                                                                                                                                                                             <  2:162775/250‑1 (MQ=255)        aCATGGGCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGC‑AAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGAt                                                                                                                                                                                                                          <  2:483377/250‑1 (MQ=255)          aTGGGCATAGTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGC‑AAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTa                                                                                                                                                                                                                        <  2:303337/250‑1 (MQ=255)           tGGGCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGC‑AAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTAc                                                                                                                                                                                                                       >  2:333333/1‑250 (MQ=255)             ggCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGC‑AAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTAccc                                                                                                                                                                                                                     <  2:322320/250‑1 (MQ=255)                                             ccaccGGAATATCATATTTATCGAGTTGGGC‑AAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCAc                                                                                                                                                                                     >  2:503953/1‑250 (MQ=255)                                                ccGGAATATCATATTTATCGAGTTGGCA‑AAAAAATGCCTCACCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTTTTTAATTTTGTTTTCGCTTTTTTGCAGATCTTCTGCCGGTTTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTGGGCGGTGATGGCAGGAAAAACTACAGAAAAAAA‑CGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCAt                                                                                                                                                                                  <  2:91061/250‑1 (MQ=255)                                                 cGGAATATCATATTTATCGAGTTGGGC‑AAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCAt                                                                                                                                                                                  >  1:429640/1‑249 (MQ=255)                                                  gaaaTATCATTTTTATCGA‑TTGGGCAAAAAAATGACTCATCCGCGGGGAAACTGAGAATAATAATCCTTTTAATCATTTTCTTCTTATTTTTGTTTCCGCTTTTTTGCAGATCTTCTGCCGGTTTGGGCGACGTTTGTAATATCCCGTCGAACCC‑TTCTTCTTCAGTTTTGGCGGTGATGGCATGCAAAACTTAAGAAAAAAA‑CGTATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATaa                                                                                                                                                                                <  2:528923/248‑1 (MQ=255)                                                             tttATCGAGTTGGGC‑AAAAAATGACTCATCCGCTGGCGATCTGAGCATAATAATGCTTTTATTCTTTTTCTGTTTATTTTTGCTTTCTCTTTTTTGCAGATCTTCTGCGGGATGGTGCGACTTTTGTAATATCCCTTCGAAACT‑TTCTTCTTCAGTTTTGGGGGTGATGGCATGCAAACCTTCAAAAAAAAA‑CGAATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCaaaa                                                                                                                                                                     <  2:78887/250‑1 (MQ=255)                                                                  cGAGTTGGGC‑AAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCg                                                                                                                                                                 >  2:25897/1‑249 (MQ=255)                                                                          cc‑AAAAAATGACTCACCCGCTGCCGAACGGAGCATAATAATCCTTTTATTCTTTTTCTGTTTATTTTTGCTTTCGCTTTTTTGCAGATCTTCTGCCGGTTTGTGCGACTTTTGAAATACCACGCCAAACCC‑TTCTTCTTCAGTTTGGGCGGTGATGGCATGAAAAACTACAAAAAAAAA‑CGAATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCa                                                                                                                                                        <  2:196162/249‑1 (MQ=255)                                                                              aaaatTGCCTCATCCGCGGGCGAACTGAGTAAAATAATGTTTTTAATCATTTTCGTTTTATTTTTTTTTTCGCTTTTTTGCAGTTTTTCTGCCGGATTGTGCGACGTTTGAAATACCCCGTAAAACCC‑TTCTTCTTCAGTTTTGGCGGTGATGGCATAAAAAACTACAGAAAAAAA‑CGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAgcg                                                                                                                                                     <  2:381064/250‑1 (MQ=255)                                                                                 aaTGACTCCTCCTCTGGCGAACTGAGCATAATAATGCTTTTAATCATTTTCTTCTTATTTTTGTTTTCGCTTTTTTGCAGATTTTCTGCCGGATTGTGCGACTTTTGTAATACCACGTCAAACCC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAC‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAgcgcgc                                                                                                                                                  <  1:368549/250‑1 (MQ=255)                                                                                         aTCCGCTGGCGAACGGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGTTTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTGGGGGGTGATGGCATGAAAAACTTCAGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGc                                                                                                                                          <  1:528687/250‑1 (MQ=255)                                                                                             gCTGGCAAACTGAGCATAATAATCCTTTTAATCATTTTCTGTTTATTTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGGGCGACGTTTGTAATATCACGTCGAACCC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAAAAAAA‑CGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGt                                                                                                                                      <  1:93427/250‑1 (MQ=255)                                                                                                 gCGAACTGAGCATAATAACGCCTTTAATCATTTTCTGCTTAATTTTGTTTTCGCATTTTTGCAGATCTTCTGCCGGATTGGGCGACGTTTGTAATATCACGTCGAACCC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTAAGAAAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGttt                                                                                                                                    <  1:528338/248‑1 (MQ=255)                                                                                                               ataatCCCTTAAATCTTTTTCTTCTTATTTTTTCTTTCTCTTTTTTGCAGATCTTCTGCCGTATTGTGCAACTTTTGAAATATCACGTCAAACCC‑TTCTTCTTCAGCTTTGGCGGTGATGTCATGAAAAACTCAAGAAAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAAtt                                                                                                                    <  2:343314/250‑1 (MQ=255)                                                                                                                        ttAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATgg                                                                                                           >  1:293538/1‑250 (MQ=255)                                                                                                                          aaTCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGcc                                                                                                         >  1:299072/1‑250 (MQ=255)                                                                                                                          aaTCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGcc                                                                                                         >  1:81496/1‑250 (MQ=255)                                                                                                                             cATTTTCTGCTTAATTTTGTTTTCGTTTTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTGGGCGGTGATGGCATGAAAAACTTCAGAAAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCg                                                                                                       <  2:504411/249‑1 (MQ=255)                                                                                                                                              tGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGGGGGCCAGCCTGCTTAGTGGCTACTGTAAATCTTCACTGGCCCGTAACACttttttcccggc                                                                                     >  2:398391/1‑243 (MQ=255)                                                                                                                                                   tCGTATTTTTGCAGATCTTCTGCGGGATTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCATCTGTGGGGGTGATGGCATGAAAACCTTAAGAAAAAAA‑CGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTcc                                                                                 <  2:148849/249‑1 (MQ=255)                                                                                                                                                    cGCTTTTTTGCAGTTCTTCGGCCGTATGGTGCGACTTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGAGGGCATAAAAACCTTAAGAAAAAAA‑CGTATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCg                                                                                <  2:383732/249‑1 (MQ=255)                                                                                                                                                        tttttGAAGTTTTTCTGCGGGTTTGTGCGAGTTTTGAAATACCAGTTGAAAACC‑TTTTTCTTAGGCTTGGGGGGGGATGGCAAGAAAAACTAGAAAAAAAAA‑CGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGga                                                                            <  2:522588/249‑1 (MQ=255)                                                                                                                                                         gtttGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGTGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATACTGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTCCTTCTTCAATGGCCCGTAACACTTTTTCGCGCGCCTCCGGGgagt                                                                          >  2:64836/2‑249 (MQ=255)                                                                                                                                                             gCATATCTTCGGCGGTTTTGGGCTACTTTTGTAATATCACGTCAAACCT‑TTTTTTTTCAGCTTGGGGGGTGATGGTAGAAAAAACTACAGAAAAAAA‑CGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGatat                                                                       <  2:121126/249‑1 (MQ=255)                                                                                                                                                              ctggttttcgGCCGGTTTGTGCGTCGTTTGTAATATCCCGTCGAACCC‑TTCTTCTTCAGCTTGGGCGGTGATGGCATGAAAAACTTAAGAAAAAAC‑CGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTa                                                                     <  2:357675/244‑1 (MQ=255)                                                                                                                                                              catttcttctGCCGGTTTGTGCGACGTTTGTAATACCACGTCGAACCC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATACAAAACTACAGAAAAAAG‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTa                                                                     <  2:15558/246‑1 (MQ=255)                                                                                                                                                                  ttttctGCGGGTTGGTGCGCCTTTTTTAATACCCCGTCAAACCC‑TTCTTCTTCAGTTTGGGGGGTGATGGCATG‑AAAACCTCAAAAAAAAACCGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGtt                                                                  <  2:94395/247‑1 (MQ=255)                                                                                                                                                                  tcttctGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGttt                                                                 >  1:264243/1‑250 (MQ=255)                                                                                                                                                                   cttctGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTg                                                                >  2:252688/1‑250 (MQ=255)                                                                                                                                                                   ctcctGCGGGTTTGTGGGCTGTTTATAATATCCCGCCGAACCC‑TTTTTTTTCAGCTTGGGGGGTGAGGGAAGAAAAAACTTAAAAAAAAAG‑CGAATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGttt                                                                 <  2:15625/246‑1 (MQ=255)                                                                                                                                                                    ttctGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGc                                                               >  1:453175/1‑250 (MQ=255)                                                                                                                                                                     tctGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCt                                                              >  1:516537/1‑250 (MQ=255)                                                                                                                                                                       tGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTa                                                            >  1:34546/1‑250 (MQ=255)                                                                                                                                                                       tGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCACGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGCGATATTAGTTTGCTTa                                                            >  2:419996/1‑250 (MQ=255)                                                                                                                                                                                gtgCGAGTTTTGAAATATCCCGACAAAACC‑TTCTTGTTCAGCTTTGGCGGGGGTGGAATGAAAAACTAAAAAAAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCAcg                                                   <  2:148195/250‑1 (MQ=255)                                                                                                                                                                                gggCGACGTTTTAAATATCACGTCGAAACC‑TTTTTCTTCACCTTTGGGGGTGAGTGCAGGCAAAACTGCAAAAAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCAc                                                    <  2:318708/247‑1 (MQ=255)                                                                                                                                                                                   cGACGTTTGTAATATCCCGTCGAACCT‑TTCTTCTTCAGCTTTGGCGGTGAGGGCAGGCAAAACTTCAGAAAAAAA‑CGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGt                                                <  1:10128/250‑1 (MQ=255)                                                                                                                                                                                      cGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGa                                              >  1:76240/1‑249 (MQ=255)                                                                                                                                                                                        tttGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATAc                                           >  2:350646/1‑250 (MQ=255)                                                                                                                                                                                             aaTACCTCGGCGAAATC‑TTCTTCTTCAGCTTGGGGGGTGATGGCATAAAAACCTTCAGAAAAAAC‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTgg                                      <  2:450669/250‑1 (MQ=255)                                                                                                                                                                                                 ccACGTCGAACCC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAAAAAAC‑CGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGAtt                                   <  1:260564/248‑1 (MQ=255)                                                                                                                                                                                                 accccttgAAACCTTTCTTCTGCACTTTGGGCGGGGGTGGCATCAAAAATTTAAGAAAAAAC‑CGGATACCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGAtt                                   <  2:248733/243‑1 (MQ=255)                                                                                                                                                                                                     gTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGa                                                                                                                              >  1:180069/1‑154 (MQ=255)                                                                                                                                                                                                        gAACCC‑TTCTTCTTCAGCTTTGGCGGTGATGGCTTGAAAACCTCCAGAAAAAAC‑CGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATAc                            <  1:503953/249‑1 (MQ=255)                                                                                                                                                                                                        gAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACg                           >  2:27770/1‑250 (MQ=255)                                                                                                                                                                                                          aaCC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGcc                         >  2:304808/1‑250 (MQ=255)                                                                                                                                                                                                            cc‑TTCTTCTGAGGTTTGGGCGGTGGTGGCAGGCAAAACTTCAGAAAAAAA‑CGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTg                       <  2:465425/250‑1 (MQ=255)                                                                                                                                                                                                               ttcttcttcAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGc                      >  2:323258/1‑249 (MQ=255)                                                                                                                                                                                                                tcttcttcAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCaa                    >  1:358179/1‑250 (MQ=255)                                                                                                                                                                                                                  ttcttcAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAAcc                  >  2:147668/1‑250 (MQ=255)                                                                                                                                                                                                                   tcttcAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAAccc                 >  2:244884/1‑250 (MQ=255)                                                                                                                                                                                                                          cTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCaa           >  1:434761/1‑249 (MQ=255)                                                                                                                                                                                                                                   tGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCAATATCATaaa  >  1:259899/1‑249 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                    AGAATAAACATGGGCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGC‑AAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACC‑TTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAA‑CGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCAATATCATAAA  >  NZ_CP009273/1377733‑1378205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑