Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,428,3610TG44.7% 65.7 / 17.0 47Y252S (TAC→TCC) truAtRNA pseudouridine(38‑40) synthase TruA
Reads supporting (aligned to +/- strand):  ref base T (5/21);  new base G (21/0);  total (26/21)
Fisher's exact test for biased strand distribution p-value = 5.28e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATTAAGAACAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATT‑CGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGTTCGGGACTGG  >  NZ_CP009273/2428114‑2428609
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                         
aTTAAGAACAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGTAAGATCATACCGGTCAGGGGaa                                                                                                                                                                                                                                                         >  2:440162/1‑250 (MQ=255)
    aGAACAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCa                                                                                                                                                                                                                                                     <  2:111687/250‑1 (MQ=255)
       aCAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATAATAACGGGCAGGGGAATccccag                                                                                                                                                                                                                                                  >  1:22262/1‑246 (MQ=255)
            aTGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGAcc                                                                                                                                                                                                                                             <  2:251006/250‑1 (MQ=255)
                  tAAACCCAGACCCCGTAACTCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAAGCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAc                                                                                                                                                                                                                                       <  2:400894/250‑1 (MQ=255)
                      cccAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATAACGGGCAGGGGAATCCACCCCGACCAGATACAGcc                                                                                                                                                                                                                                   >  1:45772/1‑250 (MQ=255)
                        cAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGATCATACCGGGCAGGGGAATCCACCCCGACCAGATAAAAcccc                                                                                                                                                                                                                                 >  1:136562/1‑249 (MQ=255)
                                gTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGTAGA‑CATATACGTCAGGGGGGTTCACCCCGACCAGATACAGCCCTTcccccct                                                                                                                                                                                                                         >  2:96197/1‑245 (MQ=255)
                                        cgACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTtg                                                                                                                                                                                                                 <  2:41983/250‑1 (MQ=255)
                                        cgACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTtg                                                                                                                                                                                                                 <  2:244569/250‑1 (MQ=255)
                                            cAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGAACATACCGGCCAGGGGAATCCACCCCGGCCAGATACACCCCTTCCCCTTTTTCCGGtgctgc                                                                                                                                                                                                             >  1:170039/1‑250 (MQ=255)
                                            cAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGTAGATCATTCCCGTCAGGGGAATCCACCCCCGCCCGATAAAACCCTTCCCCctttttccctgctgc                                                                                                                                                                                                             >  2:217214/1‑239 (MQ=255)
                                             aGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑CTTTGGGAGATCATACCGGGGCGGGGAAACCACCCCTCCCAGCAACAACCCCCCCGCGTTCTCCGTTTCTccc                                                                                                                                                                                                            >  2:77978/1‑250 (MQ=255)
                                                     cAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGt                                                                                                                                                                                                    <  2:265918/250‑1 (MQ=255)
                                                         tGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGGAAACCACCCCGACCAGATACAGCCCTTCCGCTTTTTCCGTTGCTGCCCCCCCCGTTTTg                                                                                                                                                                                                >  1:516955/1‑250 (MQ=255)
                                                                     tgCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATAACCGGCCGGGGAATCCACCCCGACCCGATAAAACCCCTCCGCTTTTTCCCTTTCTTCCCCCCGCGCTCTTTTCTTTtccccc                                                                                                                                                                                    >  2:50939/1‑250 (MQ=255)
                                                                               aaTCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATAACGGTCCGGGGAATCCACCCCGACCCGATACAGCCCCTCCGCTTTTTCCCTTTCTGCCGCCCGCGTTCTTTCCTTTTCCGCCCGCCACTCTg                                                                                                                                                                          >  2:513005/1‑250 (MQ=255)
                                                                                                        cATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTtgt                                                                                                                                                  <  2:235566/249‑1 (MQ=255)
                                                                                                               gCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATACCCGGCAGGGGAATCCACCGCCACCAGATAAAGCCCTTCCGCTTTTTTCCGTGCTCTCGCCCCCCTTCTTTCCTTTTCCCCCCCCAACTCTGCCATTCCGCTCTCCGGCTGGTTGTggggtccg                                                                                                                                          >  2:488272/1‑245 (MQ=255)
                                                                                                                       cTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATACCCGGCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTTCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCCGCCACTCTGCTATCCAGCCCTCCCGCTGGTTGTGGGGACCGACTTCCAt                                                                                                                                  >  1:61646/1‑250 (MQ=255)
                                                                                                                             cTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCt                                                                                                                            <  2:299761/250‑1 (MQ=255)
                                                                                                                                        tCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAata                                                                                                                  <  1:188951/249‑1 (MQ=255)
                                                                                                                                                tAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGa                                                                                                           <  1:455936/248‑1 (MQ=255)
                                                                                                                                                  aCTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCat                                                                                                       <  2:130619/250‑1 (MQ=255)
                                                                                                                                                           gCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATACCGGTCAGGGGAATCCACCGCGGCCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCCGCGTTCTGTCCTTTGCCGCCAGCCACTCTGCTATCCAGCTCTCCGGCTGGGTGTGGGCACCGGCTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTa                                                                                               >  1:439910/1‑249 (MQ=255)
                                                                                                                                                               cAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACaaaa                                                                                          <  1:418223/250‑1 (MQ=255)
                                                                                                                                                                aGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTTGAGATTATAACGGGCAGGGGGAACCCCCCCCACCCCATAAACACCCTCCCCTTTTGCCGCTTGTTCCGCCCGCGCCTTTTCCCTTTCCGCCCGCACCACCCCTATCCCACCCCCCCCGTGGGTGTGGGGACCCCACGCCATCCAGCAGCCGCCCAAATTCCTGACCCCTAGATGTACaaaa                                                                                          >  2:213551/1‑249 (MQ=255)
                                                                                                                                                                    tGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATTATACCCGTCAGGGGAAACCACCGCGACCAGATACAGCCCCTCCGCTTTTTCCGTTGCTGCCGCCCGCGGTCTGTCCCTTGCCGCCAGCAACCCTTCTATCCAGCTCTCCGGCTGGTTGTGGGCGCCGCCTTCCATCAGGCTGCCGCCAAAATTCCTTACCCTATGATGTAAAAAAACAtt                                                                                     >  2:135328/1‑250 (MQ=255)
                                                                                                                                                                       aaTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCATACCGGTCAGGGGAATCCCCCGCGACCCGATACAGCCCTTCCCCTTTTTCCGTTTCTGCCGCCAGCGTTCTGTCCTTTTCCCCCAGCAACTCTTCTATCCCACTCTCCCGCTGGGTGTGGGCACCCACTTCCCTCAGGCTGCCGCCAATATTTCTGACCATATGATGTACAAAAACATT‑CGc                                                                                 >  1:462380/1‑250 (MQ=255)
                                                                                                                                                                               gAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATT‑CGCTTTAatat                                                                         <  1:28964/250‑1 (MQ=255)
                                                                                                                                                                                  cATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATT‑CGCTTTAATATCTa                                                                      <  2:22378/250‑1 (MQ=255)
                                                                                                                                                                                     tattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAGCCCCTCCCCTTTTGCCGTTGCTGCCCCCCGCGTTCTGTCCTTTTCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATT‑CGCTTTAATATCTacca                                                                   >  1:191653/1‑250 (MQ=255)
                                                                                                                                                                                      attCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCATACCCGGCAGGGGAAACCACCCCGACCAGAAACAGCCCCTCCGCCTTTTCCGTTGCTGCCCCCCGCGGTCTGTCCTTTTCCGCCCGCAACTCTGCTATCCCGCTCTCCGGCTGGGTGTGGGGACCGACTTTCATCAAGGTGCCGACAAAATTTCTGACCATAATATGGACAAAAGAAAT‑TGCTTTTAAATCTaccac                                                                  >  2:148162/1‑250 (MQ=255)
                                                                                                                                                                                        tctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑GTTTTGGAGATCATACCCGGCAGGGGAATACACCCCGACCCGATACAGCCCCTTCCCCTTTGCCGTTGCTGCCGCCCCCGTTCTTTGCTTTGCCGCCAGCCAACCTGCCATCCACCCCTCCGGCTGGGTGTGGGGACCCACTTCCCTCAGGCTGCCGCCCAAATTTCTGACCATATGGTTTACAAAAGCCTTCCGCTTTATTAACTcccacc                                                                 >  2:279379/1‑250 (MQ=255)
                                                                                                                                                                                         ctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATACCGGGCCGGGGAATCCACCCCGACCAGATACAGCCCCTCCCCCTTTTCCGTTTCTGCCCCCCGCGGTCTGTCCTTTGCCCCCCGCAACCCTGCTATCCCGCCCTCCCGCTGGTTGGGGGGACCCGCCTCCCTCAGGGCGCCCGCAATATTCCTTGCCATATGGAGTACAAAAACATT‑TCCCTTTATATCTaacacccc                                                               >  2:484043/1‑248 (MQ=255)
                                                                                                                                                                                             ttAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATT‑CGCTTTAATATCTACCACCACATaa                                                           <  2:1020/250‑1 (MQ=255)
                                                                                                                                                                                                gTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGT‑GGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATT‑CGCTTTAATATCTACCACCACATAAGGAc                                                       <  2:285063/250‑1 (MQ=255)
                                                                                                                                                                                                        gAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATT‑CGCTTTAATATCTACCACCACATAAGGACCGTGAcg                                                <  1:437994/250‑1 (MQ=255)
                                                                                                                                                                                                                 ggCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATT‑CGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACAtt                                       <  2:226865/250‑1 (MQ=255)
                                                                                                                                                                                                                   cccATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATT‑CGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTaa                                     <  2:342971/250‑1 (MQ=255)
                                                                                                                                                                                                                       ttGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATT‑CGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATg                                 <  2:92825/250‑1 (MQ=255)
                                                                                                                                                                                                                       ttGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGGTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATT‑CGCTTTAATATCTACCACCACATAAGGACCGTGAAGCGGTAAATTAATATg                                 >  2:227861/1‑250 (MQ=255)
                                                                                                                                                                                                                                        aTCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATT‑CGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCAc                 >  2:167790/1‑250 (MQ=255)
                                                                                                                                                                                                                                              ccGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATT‑CGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGtt           >  2:29870/1‑250 (MQ=255)
                                                                                                                                                                                                                                                ggTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATT‑CGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGTTCg         >  2:111612/1‑250 (MQ=255)
                                                                                                                                                                                                                                                   cAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATT‑CGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGTTCGGGa      >  1:397225/1‑250 (MQ=255)
                                                                                                                                                                                                                                                       gTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATT‑CGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGTTCGGGACTgg  <  1:287127/250‑1 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                         
ATTAAGAACAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATT‑CGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGTTCGGGACTGG  >  NZ_CP009273/2428114‑2428609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑