Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,913,385	T→G	V540G (GTT→GGT)	yebT →	lipid‑binding membrane homeostasis protein YebT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,913,385	0	T	G	100.0%	199.4 / NA	50	V540G (GTT→GGT)	yebT	lipid‑binding membrane homeostasis protein YebT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (24/26);  total (24/26)

AAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTAT  >  NZ_CP009273/1913144‑1913631                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       aaGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAg                                                                                                                                                                                                                                                <  2:266568/250‑1 (MQ=255)   ggTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGt                                                                                                                                                                                                                                               >  1:449973/1‑249 (MQ=255)   ggTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGGAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGt                                                                                                                                                                                                                                               >  1:485045/1‑249 (MQ=255)       gggCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGAtt                                                                                                                                                                                                                                           <  1:126373/249‑1 (MQ=255)        ggCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTAc                                                                                                                                                                                                                                         <  2:85160/250‑1 (MQ=255)        ggCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTAc                                                                                                                                                                                                                                         <  1:294103/250‑1 (MQ=255)          cTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGGGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGGGAAGTGATTACCg                                                                                                                                                                                                                                       >  1:421621/1‑250 (MQ=255)                   gTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGGATTCTGCCGGGCGGTAAAGGGGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGAACGGTAGTGGTGGACCGTAAATTTGAAGGGGGGGGAGTGGTTACCGTGCGTCcgc                                                                                                                                                                                                                              >  2:395839/1‑250 (MQ=255)                      gAGTGTCTCAGGGCCAGCGCCTCAGAATGGACCGATGGCGGGATCCGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGGTAGCCACGGTATGCCAAGTTGGAAAAAGCGCTAGAAAACAGCAGTAGGGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAAACGCTGGAGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAg                                                                                                                                                                                                                           <  2:144222/250‑1 (MQ=255)                       aGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGGGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTTGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGGAGTGCTGTACCGGAAATTTGAAGGGGGTGAAGGGATTAACGTGCGTCCGCGAGc                                                                                                                                                                                                                          >  2:371470/1‑250 (MQ=255)                          ttCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATAAGTATTCTGGCGGGCGATAAAGGGGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGGGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGGGAGTTTGAGGGCAGAGAAGGTGCCGGATGGGCAGGCAGGAACGGGAGTGGTGTACCGGAAATTTGAAGGGGGGGAAGGGGTTACCGTGCGTGCGCGGGCTa                                                                                                                                                                                                                        >  2:360680/1‑249 (MQ=255)                            ctcGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAAcg                                                                                                                                                                                                                     >  1:288404/1‑250 (MQ=255)                               ggTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAAcgcg                                                                                                                                                                                                                   <  1:184470/249‑1 (MQ=255)                                           tCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGGGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCCGCCGGATGTGCAGGCAGGATCGGGAGTGCTGTACCGTAAATTTGAAGGGGGGGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGGTc                                                                                                                                                                                                      >  2:446052/1‑250 (MQ=255)                                             aGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATc                                                                                                                                                                                                      <  1:497209/248‑1 (MQ=255)                                                     tCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTaa                                                                                                                                                                                            <  2:360965/250‑1 (MQ=255)                                                          ggCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCgg                                                                                                                                                                                       <  2:150444/250‑1 (MQ=255)                                                            cGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAg                                                                                                                                                                                     <  1:96537/250‑1 (MQ=255)                                                               gATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTAt                                                                                                                                                                                  >  1:302343/1‑250 (MQ=255)                                                                   cGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCa                                                                                                                                                                              <  1:408590/250‑1 (MQ=255)                                                                     tATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAAc                                                                                                                                                                            <  1:342475/250‑1 (MQ=255)                                                                              gggCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGAc                                                                                                                                                                    >  2:467784/1‑249 (MQ=255)                                                                                gCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAg                                                                                                                                                                 <  1:282641/250‑1 (MQ=255)                                                                                                 aaaGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTggg                                                                                                                                                <  1:237615/250‑1 (MQ=255)                                                                                                       aGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAg                                                                                                                                          <  1:462199/250‑1 (MQ=255)                                                                                                          tATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGACACGCTGTCGGATGTGCAGGCACGATCGGTAGCGCAGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGGTACGCAACCTTCTGACCAGCAGTAGCCGGTTCTGGGCAGAAGGCg                                                                                                                                       >  2:54117/1‑250 (MQ=255)                                                                                                                       ccAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTc                                                                                                                           <  1:374295/249‑1 (MQ=255)                                                                                                                        cAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAg                                                                                                                         >  2:3942/1‑250 (MQ=255)                                                                                                                                 aaaaGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGt                                                                                                                <  1:300142/250‑1 (MQ=255)                                                                                                                                        cTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTc                                                                                                         >  2:72726/1‑250 (MQ=255)                                                                                                                                            agaACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGAc                                                                                                     <  2:29017/250‑1 (MQ=255)                                                                                                                                                         cGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATcc                                                                                        <  1:93154/250‑1 (MQ=255)                                                                                                                                                          gATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATccc                                                                                       >  2:206060/1‑250 (MQ=255)                                                                                                                                                                    ccacAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCtctc                                                                                >  1:7640/1‑247 (MQ=255)                                                                                                                                                                    ccacAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCag                                                                             >  2:132699/1‑250 (MQ=255)                                                                                                                                                                     cacaACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCaga                                                                            <  2:270634/250‑1 (MQ=255)                                                                                                                                                                        aaCCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGCTCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGGGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGGGCa                                                                         >  2:455562/1‑250 (MQ=255)                                                                                                                                                                               aGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAg                                                                   <  1:216922/249‑1 (MQ=255)                                                                                                                                                                                gTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAggg                                                                 <  2:222815/250‑1 (MQ=255)                                                                                                                                                                                 tttGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAgg                                                                  >  1:427942/1‑248 (MQ=255)                                                                                                                                                                                            agaCGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCtt                                                     >  1:303610/1‑250 (MQ=255)                                                                                                                                                                                                      ggATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTc                                           <  1:95313/250‑1 (MQ=255)                                                                                                                                                                                                           tgCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCgg                                      >  2:204612/1‑250 (MQ=255)                                                                                                                                                                                                                gcaggATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGc                                   >  2:345696/1‑248 (MQ=255)                                                                                                                                                                                                                 caggATCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAg                                >  1:82988/1‑250 (MQ=255)                                                                                                                                                                                                                      tCGGTAGTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAgcgc                             >  2:3829/1‑248 (MQ=255)                                                                                                                                                                                                                            gTGCTGTACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGc                     >  1:251146/1‑250 (MQ=255)                                                                                                                                                                                                                                  tACCGTAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAg               <  1:395839/250‑1 (MQ=255)                                                                                                                                                                                                                                       tAAATTTGAAGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGAt          <  1:335820/250‑1 (MQ=255)                                                                                                                                                                                                                                               aaGGTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTAt  <  1:467784/250‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       AAGGTGGGGCTGGATGGCGTTGAGTTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTTAGCGATTTACCCACCACAACCGTGAGTTTGAGTGCAGAGACGCTGCCGGATGTGCAGGCAGGATCGGTAGTGCTGTACCGTAAATTTGAAGTTGGTGAAGTGATTACCGTGCGTCCGCGAGCTAACGCGTTTGATATCGATCTGCATATTAAGCCGGAGTATCGCAACCTTCTGACCAGCAATAGCGTGTTCTGGGCAGAAGGCGGGGCGAAAGTTCAGCTGAATGGTAGTGGTCTGACCGTACAGGCATCCCCGCTCTCCAGAGCATTAAAGGGAGCCATTAGCTTCGACAACCTCAGCGGTGCCAGCGCCAGTCAGCGTAAAGGCGATAAGCGTAT  >  NZ_CP009273/1913144‑1913631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑