Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 4,323,527 G→T R81S (CGC→AGC)  basR ← two‑component system response regulator BasR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,323,5270GT98.0% 199.5 / ‑5.9 51R81S (CGC→AGC) basRtwo‑component system response regulator BasR
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (25/25);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

ATTAACCGTGACAGCAGAGCATATTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCGAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTAATGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATAACAGCGTATCGTCTTCAACAATCAGAATTTTCATTCACT  >  NZ_CP009273/4323283‑4323772
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     
aTTAACCGTGACAGCAGAGCATATTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCgg                                                                                                                                                                                                                                                  >  1:468139/1‑250 (MQ=255)
   aaCCGTGACAGCAGAGCATATTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGt                                                                                                                                                                                                                                                 >  1:258510/1‑248 (MQ=255)
   aaCCGTGACAGCAGAGCATATTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATAGCTAGCGGTgc                                                                                                                                                                                                                                               >  2:338599/1‑249 (MQ=255)
     ccGTGACAGCAGAGCATATTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAgg                                                                                                                                                                                                                                             >  2:66972/1‑250 (MQ=255)
        tGACAGCAGAGCATATTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATc                                                                                                                                                                                                                                          <  1:511042/250‑1 (MQ=255)
         gACAGCAGAGCATATTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCa                                                                                                                                                                                                                                         <  2:64767/250‑1 (MQ=255)
               agagCATATTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCACGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGt                                                                                                                                                                                                                                       >  1:422026/1‑246 (MQ=255)
                gagCATATTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCACGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTa                                                                                                                                                                                                                                      <  2:422026/246‑1 (MQ=255)
                 agCATATTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCgg                                                                                                                                                                                                                                  >  1:206919/1‑249 (MQ=255)
                      atTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGTCGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGCCGCGGAAACGGGCATGTAACTTTTCCAGCGCAAAACGCTTCAACAGATAGTCCTCGGCACCGACATCCAGCGCGGCGATTTTGTCGGTTAGCGTATTGCGACCGGTCAGGACCAGTACCGACAggg                                                                                                                                                                                                                             >  2:379692/1‑249 (MQ=255)
                      atTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACAGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAggg                                                                                                                                                                                                                             >  1:55946/1‑249 (MQ=255)
                       ttCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGta                                                                                                                                                                                                                           <  2:169077/250‑1 (MQ=255)
                                        tCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGa                                                                                                                                                                                                           >  2:270919/1‑249 (MQ=255)
                                                   ccGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCgaga                                                                                                                                                                                                >  1:357040/1‑249 (MQ=255)
                                                     gCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGaaaa                                                                                                                                                                                             >  2:125753/1‑250 (MQ=255)
                                                           ccatACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGt                                                                                                                                                                                       >  1:72515/1‑250 (MQ=255)
                                                                 cTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCt                                                                                                                                                                                 <  2:109395/250‑1 (MQ=255)
                                                                    aCGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTtc                                                                                                                                                                               <  1:476157/249‑1 (MQ=255)
                                                                     cGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTtcgt                                                                                                                                                                             <  1:374358/250‑1 (MQ=255)
                                                                        cGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCgg                                                                                                                                                                          >  1:68966/1‑250 (MQ=255)
                                                                                   cAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTaa                                                                                                                                                                <  1:328633/249‑1 (MQ=255)
                                                                                    aGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAAt                                                                                                                                                              <  2:403770/250‑1 (MQ=255)
                                                                                          aGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTa                                                                                                                                                        <  2:501691/250‑1 (MQ=255)
                                                                                             ttGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTacca                                                                                                                                                     >  2:69594/1‑250 (MQ=255)
                                                                                                      aTCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGt                                                                                                                                            <  1:358216/250‑1 (MQ=255)
                                                                                                        cAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTaa                                                                                                                                          >  2:43851/1‑250 (MQ=255)
                                                                                                               cTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTGCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTAATGACCTg                                                                                                                                   <  2:92649/250‑1 (MQ=255)
                                                                                                                 ttCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTAATGACCTGcc                                                                                                                                 >  1:440066/1‑250 (MQ=255)
                                                                                                                  tCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTAATGACCTGc                                                                                                                                  <  1:463532/248‑1 (MQ=255)
                                                                                                                     cccTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTAATGACCTGCCTCaa                                                                                                                             <  2:226165/250‑1 (MQ=255)
                                                                                                                          attattATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTAATGACCTGCCTCAAGGCtt                                                                                                                        <  1:95789/250‑1 (MQ=255)
                                                                                                                              ttatGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTAATGACCTGCCTCAAGGCTTTGtt                                                                                                                    <  1:197362/250‑1 (MQ=255)
                                                                                                                                   gCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTAATGACCTGCCTCAAGGCTTTGTTCCGc                                                                                                                >  1:158893/1‑249 (MQ=255)
                                                                                                                                    cgtcgtAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGGACCGGCAGGGTATATTTTTTCTGCCGGATACGGGGGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCCAAATCCCAGACCAACAGGCTGTAATTACCTGCCTCAAGGCTTTGTTCcccc                                                                                                               >  2:396060/1‑249 (MQ=255)
                                                                                                                                     gtcgtAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTAATGACCTGCCTCAAGGCTTTGTTCCGCCAt                                                                                                             <  1:284808/250‑1 (MQ=255)
                                                                                                                                      tcgtAGCAGGGCGCGGATACGGGCATGTAACTCTGCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTAATGACCTGCCTCAAGGCTTTGTTCCGCCATc                                                                                                            <  2:57445/250‑1 (MQ=255)
                                                                                                                                            cAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTAATGACCTGCCTCAAGGCTTTGTTCCGCCATCcgcgcg                                                                                                      <  2:396222/250‑1 (MQ=255)
                                                                                                                                                                gTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTAATGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCAcgc                                                                                  >  1:26979/1‑250 (MQ=255)
                                                                                                                                                                  aaCTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTAATGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCAcgcg                                                                                 >  1:530800/1‑249 (MQ=255)
                                                                                                                                                                            gcgcAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTAATGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGt                                                                      <  2:154045/250‑1 (MQ=255)
                                                                                                                                                                                aaaaGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTAATGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTgc                                                                  >  1:78997/1‑250 (MQ=255)
                                                                                                                                                                                aaaaGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTAATGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTgc                                                                  <  2:206919/250‑1 (MQ=255)
                                                                                                                                                                                aaaaGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTAATGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTgc                                                                  <  1:108993/250‑1 (MQ=255)
                                                                                                                                                                                            cAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTAATGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAAt                                                       >  1:216217/1‑249 (MQ=255)
                                                                                                                                                                                                      tcgGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTAATGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGc                                             >  1:99479/1‑249 (MQ=255)
                                                                                                                                                                                                       cgGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCCTCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTAATTACCTGCCTCAAGGCTTTGTTCCGCCCTCCCCGCGGTCGTCACCCCATCGCCCGCGTAGCCTTCGGTTTGCGCCCCCAGAATCCGTCCCTGCaa                                           >  2:332914/1‑250 (MQ=255)
                                                                                                                                                                                                           aCCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTAATGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATAAc                                       <  2:468139/250‑1 (MQ=255)
                                                                                                                                                                                                                               ttttGTCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTAATGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATAACAGCGTATCGTCTTCAACAAt                   <  1:82866/250‑1 (MQ=255)
                                                                                                                                                                                                                                    tCGGTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTAATGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATAACAGCGTATCGTCTTCAACAATCAGa               <  1:175361/249‑1 (MQ=255)
                                                                                                                                                                                                                                      ggTCAGCGTATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTAATGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATAACAGCGTATCGTCTTCAACAATCAGAAtt            >  1:26854/1‑250 (MQ=255)
                                                                                                                                                                                                                                              tATCGCTAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTAATGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGGTTGCGCCGCCAGAATCAGTCCCTGCAATAACAGCGTATCGTCTTCAACAATCAGAATTttcattca    >  2:95965/1‑250 (MQ=255)
                                                                                                                                                                                                                                                 cgctAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTAATGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATAACAGCGTATCGTCTTCAACAATCAGAATTTTCATtcact  >  2:318722/1‑249 (MQ=255)
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ATTAACCGTGACAGCAGAGCATATTCTTTGGGCGTCAGAATCAACTCTTCACCGCCCATCCATACCTGACGGCGACCCATGTTCAGCGTCAGATTGCCAACAATCAGCTCACTTTCGCCCTGATTATTATGGCGTCGTAGCAGGGCGCGGATACGGGCATGTAACTCTTCCAGCGCAAAAGGCTTCACCAGATAGTCGTCGGCACCGACATCCAGCCCGGCGATTTTGTCGGTCAGCGTATCGCGAGCGGTGAGGATCAGTACCGGCAGGGTATATTTTTTCTGCCGGATACGGGCGAGAAAATGCAGTCCATCTTCGTCGGGTAACCCTAAATCCAGTACCACCAGGCTGTAATGACCTGCCTCAAGGCTTTGTTCCGCCATCCGCGCGGTTGTCACGCTATCGCACGCGTAGCCTTCGGTTTGCGCCGCCAGAATCAGTCCCTGCAATAACAGCGTATCGTCTTCAACAATCAGAATTTTCATTCACT  >  NZ_CP009273/4323283‑4323772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑