Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,221,7540TC37.0% 72.0 / 14.0 55pseudogene (484/1436 nt)mdtQmultidrug resistance outer membrane protein MdtQ
Reads supporting (aligned to +/- strand):  ref base T (20/14);  new base C (6/14);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 5.21e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCG‑CCGCCCGTGC‑TTTA‑ACCGTA‑CCCAGGC‑GGGCAGT‑AACCTCCGCCCGATTCTTT‑‑CCCCAGATA‑TC‑GAGATGCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCG  >  NZ_CP009273/2221507‑2222003
                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                  
ccGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCG‑CCGCCCGTGC‑TTTA‑ACCGTA‑CCCAGGC‑GGGCAGT‑AACCTc                                                                                                                                                                                                                                                                   <  1:167255/249‑1 (MQ=255)
   ggTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCG‑CCGCCCGTGC‑TTTA‑ACCGTA‑CCCAGGC‑GGGCAGT‑AACCTCCGcc                                                                                                                                                                                                                                                               <  1:667043/250‑1 (MQ=255)
        tGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCG‑CCGCCCGTGC‑TTTA‑ACCGTA‑CCCAGGC‑GGGCAGT‑AACCTCCGCCCGAt                                                                                                                                                                                                                                                           <  1:375350/249‑1 (MQ=255)
         gCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTTCTCGCGTTCCG‑CCGCCCCGGC‑TTTT‑ACCGTA‑CCCAGGG‑GGGGAGG‑AAACTCCCCCCgcgtc                                                                                                                                                                                                                                                         >  1:976576/1‑246 (MQ=255)
          cTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCG‑CCGCCCGTGC‑TTTA‑ACCGTA‑CCCAGGC‑GGGCAGT‑AACCTCCGCCCGATTCt                                                                                                                                                                                                                                                        <  1:926954/250‑1 (MQ=255)
             gCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCG‑CCGCCCGTGC‑TTTA‑ACCGTA‑CCCAGGC‑GGGCAGT‑AACCTCCGCCCGATTCtt                                                                                                                                                                                                                                                       <  1:703989/248‑1 (MQ=255)
             gCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCG‑CCGCCCGTGC‑TTTA‑ACCGTA‑CCCAGGC‑GGGCAGT‑AACCTCCGCCCGATTCTTT‑‑c                                                                                                                                                                                                                                                   <  2:174028/250‑1 (MQ=255)
                ttAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTGCTCGCGTTCCG‑CCGCCCGTGC‑TTTT‑ACCGCA‑ACCAGGC‑GGGCAGT‑AACCTCCGCCCGATTTTTT‑‑tccc                                                                                                                                                                                                                                                >  1:928860/1‑250 (MQ=255)
                  aaTATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCG‑CCGCCCGTGC‑TTTA‑ACCGTA‑CCCAGGC‑GGGCAGT‑AACCTCCGCCCGATTCTTT‑‑CCCCa                                                                                                                                                                                                                                               <  2:533047/249‑1 (MQ=255)
                      tCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTTCTCGCCTTCCGCCCCCCCGGGC‑TTTA‑ACCGAA‑CCCAGCC‑GGGCGGA‑ACCCCCCCCCCGATTCTTT‑‑cccccatat                                                                                                                                                                                                                                           >  1:89324/1‑245 (MQ=255)
                                cgccTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCG‑CCGCCCGTGC‑TTTA‑ACCGTA‑CCCAGGC‑GGGCAGT‑AACCTCCGCCCGATTCTTT‑‑CCCCAGATA‑TC‑GAGATGCCa                                                                                                                                                                                                                              <  1:23090/248‑1 (MQ=255)
                                cacCTTCAACTGAAGAAGTGATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCG‑CCGCCCGTGC‑TTTA‑ACCGTA‑CCCAGGC‑GGGCAGT‑AACCTCCGCCCGATTCTTT‑‑CCCCAGATA‑TC‑GAGATGCCa                                                                                                                                                                                                                              <  1:366601/250‑1 (MQ=255)
                                cacCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTAAGCTGCCAGCCAGCAATTGGCGGGGTTGCTCGCGGTCTG‑GCGCCCGGGC‑TCTA‑AAAGGA‑ACCCGGC‑GGGCGGG‑AACCACCCGCCCACTCTTT‑‑TCCCAACTA‑AC‑GCGATgg                                                                                                                                                                                                                                >  2:764830/1‑247 (MQ=255)
                                   cTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCCGCCAGCAATTGGCTGGGTTGGTCGGGTTCCG‑CCCCCCGCGG‑TTTA‑AACGTA‑CCCCGGCGGGGCGGA‑AACCCCCGCCCAATT‑TTT‑‑CCCCCGATA‑Tcacggatgcagac                                                                                                                                                                                                                            >  2:867538/1‑238 (MQ=255)
                                   cTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCG‑CCGCCCGTGC‑TTTA‑ACCGTA‑CCCAGGC‑GGGTAGT‑AACCTCCGCCCGATTCTTT‑‑CCCCAGATA‑TC‑GAGATGCCA‑Gcc                                                                                                                                                                                                                          <  1:137295/250‑1 (MQ=255)
                                                             tGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTTGTCGCGGTTCG‑CCCCCCGCGC‑TTTT‑ACCCTA‑CCCAGGC‑GGGCGGT‑AACCTCCGCCCCCGTCTTT‑‑TCCCCGATA‑TC‑GCGATGCCA‑GCCCC‑CCCCTTAGCCCAAAAGACCG‑CTgg                                                                                                                                                                                              >  2:195712/1‑250 (MQ=255)
                                                             tGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACACTGCCAGCCAGCAATTGGTGGGGTTGCTCGCGTTCCG‑CCGCCCGTGC‑TTTA‑ACCGTA‑CCCCGGC‑GGGCAGT‑AACCTCCCCCCGATTCTTT‑‑TCCCAGATA‑TC‑GAGAGGCCA‑GCCCG‑CCGTGTAACCAAAAGAACCG‑TTgg                                                                                                                                                                                              >  2:569680/1‑250 (MQ=255)
                                                                tataGTGGGCGGTCGGTCGCGATAAGGGTGTTCTGCTCTTTTTCTATTGGCTGCAAGACCGTGTTTACCGCCGCCTGGGTTTGCCCCCCCCAGTACGGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCG‑CCGCCCGTGC‑TTTA‑ACCGTA‑CCCAGGC‑GGGCAGT‑AACCTCCGCCCGATTCTTT‑‑CCCCAGATA‑TC‑GAGATGCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGgtgt                                                                                                                                                                                           <  1:148901/250‑1 (MQ=255)
                                                                    gCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTGCTCGCGGTCCG‑CCCCCCGGGC‑CTTA‑ACCGTA‑CCCCGGC‑GGGCAGG‑AAACTCCGCCCGGTTCTTT‑‑CCCCCGATA‑TC‑GAGAAGGCA‑GGCCC‑CCGGTAAGCCAAAAATAACG‑TTGGTGTAACa                                                                                                                                                                                       >  1:195518/1‑250 (MQ=255)
                                                                    gCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGCTTTCTCGCGGTCCC‑CCCCCCCGGC‑CTTT‑ACCCGA‑CCCCCGC‑GGGCGGG‑AAACTCCCCCCCGCTCTTT‑‑TCCCCGCTA‑TC‑GAGATGCCC‑GCCCGCCCGTTAACCCCAAAGACCCG‑TTGgtttccc                                                                                                                                                                                        >  2:471400/1‑245 (MQ=255)
                                                                        gCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGAAAGACCGTTTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATGGGCGGGTTTGCTCGCGTTCCG‑CCGCCCGTGC‑TTTA‑ACCGTC‑CCCAGGC‑GGGCAGT‑AACCTCCGCCCGATTCTTT‑‑CCCCAGATA‑TC‑GAGATGCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACggg                                                                                                                                                                                   <  1:874602/250‑1 (MQ=255)
                                                                         cGATCGGTCGCGATAAGGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTACCGCCGCCTGGTTTTGCCACCCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCG‑CCGCCCGTGC‑TTTA‑ACCGTA‑CCCAGGC‑GGGCAGT‑AACCCCCGCCCGATTCTTT‑‑CCCCAGATA‑TC‑GAGATGCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGc                                                                                                                                                                                  <  1:225649/250‑1 (MQ=255)
                                                                                           gtgtTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTTCTCGCGTTTCG‑CCCCCCCGGCTTTTA‑ACCGAA‑CCC‑GGC‑GGGGAGT‑AAACCCCCCCCCGTTCTTTTCCCCCAATTA‑TG‑GA‑TGGCCG‑CCCCC‑CCCTTAACCAAAAAGACCCG‑TTGGGGT‑CCCCGGGCCGGGCGGACCCG‑CGGccc                                                                                                                                                               >  2:563760/1‑249 (MQ=255)
                                                                                                cTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCCCCCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTGGCTCGCGTTCCG‑CCCCCCGTGC‑TTTA‑CCCGTC‑CCCAGGC‑GGGCAGT‑ACCCCCCGCCCGATTCTTT‑‑CCCCAGATA‑TC‑GAGATGCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATc                                                                                                                                                           <  1:27974/249‑1 (MQ=255)
                                                                                                   cttttttttTATTCGCTCCAAGCCCGTGTTAACCCCCGCCGGGTTTTGCCCCTCCCAGTACAGGCGGGCTCCGCTCCCAGCCAGAAATTGGGGGGTTTGCTCGCGTTCCG‑CCGCCCGTGC‑TTTA‑CCCGTA‑CCCGGGG‑GGGCAGT‑ACCCCCCGCCCGATTCTTT‑‑CCCCAGATA‑TC‑GAGATGCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATCGtt                                                                                                                                                        <  2:247129/246‑1 (MQ=255)
                                                                                                    tctTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTGCTCGCGTTTCG‑CCGCCCGTGC‑TTTT‑AACGTA‑CCCAGGC‑GGGCAGT‑AACCTCCGCCCGATTCTTT‑‑CCCCCGATA‑TC‑GAGATGCCA‑GCCCG‑CCGGTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTAACTG‑CGGCCGGATCgct                                                                                                                                                        >  1:504493/1‑246 (MQ=255)
                                                                                                       ttttCTATTTGCTGCAAGCCCGTGTTAAACCCCGCCTGGGTTTGCCCCTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTCCCG‑CCGCCCGTGC‑TTTA‑ACCGTC‑CCCAGGC‑GGGCAGT‑ACCCCCCGCCCGATTCTTT‑‑CCCCAGATA‑TC‑GAGATGCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATCGTTCaga                                                                                                                                                    <  1:195712/249‑1 (MQ=255)
                                                                                                            tATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTTCCCGCGTTCCC‑CCCCCCGCGC‑CTTA‑ACCGCA‑CCCAGGC‑GGGGACG‑AACCCCCCCCCCCATCTTT‑‑TCCCCGCTA‑AC‑GCGATACCACGCCCC‑CCGTCATCCCAAAA‑CACCC‑TCGTGGTTCCACGGGCCGGGCGGTCCTG‑CGGCCCGGGCGTTcgagcgaga                                                                                                                                               >  2:610188/1‑241 (MQ=255)
                                                                                                             aTTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTTCTCGCCGTCCC‑CCCCCCCCGC‑TTTT‑ACCCCA‑CCCCCGG‑GGGGAGTGAACCCCCCCCCGTTTCTTT‑‑CCCC‑CATATTC‑GGGAGACCA‑CCCCC‑CCGTGAAGCCAAAAG‑ACCGCTGGGTGTACCACGGGCCGGT‑GTCCCCGCCGGCCGGATCCttacgggacacc                                                                                                                                              >  2:24251/1‑240 (MQ=255)
                                                                                                               ttGCTCCAACCCCGTTTTTACCGCCCCCTGGTTTTGCCCCCCCCAGGACAGGCGGCCTACGCTCCCAGCCAGCAATGGGCGGTTTTGCTCCCGTCCCC‑CCGCCCGTTC‑TTTA‑ACCGTC‑CCCGGGC‑GGGCAGT‑ACCCCCCGCCCTATTCTTC‑‑CCCCAGATA‑TC‑GAGATCCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATCGTTCAGAGCAAACgg                                                                                                                                            <  2:195518/249‑1 (MQ=255)
                                                                                                                   tgcAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTAAGCTGCCAGCCAGCAATTGGCGGGTTTGTTCGCGGTCCC‑CCGCCCCCGC‑TTTT‑ACCCTA‑CCCCGGC‑GGGGAGG‑AAACCCCGCCCGCGTCTTT‑‑TCCCCGATA‑TC‑GAGAAGCCC‑GCCCC‑CCCGCTAGCCACAAGTACCC‑GTGGTGGTCCCCCAGCCCGCTGCACCTT‑CGGCCGGGGCGTGCTGAGGACACGGccccc                                                                                                                                       >  2:589030/1‑249 (MQ=255)
                                                                                                                                                            gTACAGTCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCG‑CCGCCCGTGC‑TTTA‑ACCGTA‑CCCAGGC‑GGGCAGG‑AAACTCCGCCCGATTCTTT‑‑CCCCCGATA‑TC‑GAGATGCCA‑GCCCC‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGGCGTACCTG‑CCGGCGGATCGTTCAAGGCAAACGGCCCCCTTTAGCCTTCTGCCGACATTTTTTTGCCCCTCCactccgcc                                                                                              >  2:58457/1‑243 (MQ=255)
                                                                                                                                                               cgggCGGGCTCCGCTGCCAGCCAGCATTGGGCGGGTTTGCCCGCTTCCCG‑CCCCCCGTGC‑TTTA‑CCCGTC‑CCCGGGC‑GGGCAGT‑ACCCCCCCCCCGATTCTTC‑‑CCCCAGATA‑TC‑GAGATGCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACA‑TTTTTTGCCGCTCCATATCCGCCGaa                                                                                          <  1:563760/248‑1 (MQ=255)
                                                                                                                                                               cAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCG‑CCGCCCGTGC‑TTTA‑ACCGTA‑CCCAGGC‑GGGCAGT‑AACCTCCGCCCGATTCTTT‑‑CCCCCGATA‑TC‑GAGATGCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCCCGGGCCGGTCGTACCTG‑CGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACC‑TTTTTTGCCGCTCCCTATCCGCCGa                                                                                           >  1:668549/1‑249 (MQ=255)
                                                                                                                                                                   cGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCG‑CCGCCCGTGC‑TTTA‑ACCGTA‑CCCAGGC‑GGGCAGT‑AACCTCCGCCCGATTCTTT‑‑CCCCAGATA‑TC‑GAGATGCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGt                                                                                      >  1:541869/1‑250 (MQ=255)
                                                                                                                                                                       cTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCG‑CCGCCCGTGC‑TTTA‑ACCGTA‑CCCAGGC‑GGGCAGT‑AACCTCCGCCCGATTCTTT‑‑CCCCAGATA‑TC‑GAGATGCCC‑GCCCC‑CCCTTAAGCCAAAAATACCG‑GTGGTGTACCACGGGCCGGTCGTCCCCT‑CGGCCCGGTCGTTCAGAGCAAACGGCCCCACTAAGCCTTCTGCCCACC‑TTTTTTGCCGCTCCATATCCGCCGCACAGTCGaa                                                                                  >  2:550835/1‑249 (MQ=255)
                                                                                                                                                                       cTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCG‑CCGCCCGTGC‑TTTA‑ACCGTA‑CCCAGGC‑GGGCAGT‑AACCTCCGCCCGATTCTTT‑‑CCCCAGATA‑TC‑GAGATGCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGAt                                                                                  >  1:236156/1‑250 (MQ=255)
                                                                                                                                                                          cGCTCCCAGCCAGCATTTGGGGGGTTTCCTCGCGTTCCG‑CCGCCCGTGC‑TTTA‑CCCGTC‑CCCAGGC‑GGGCAGT‑ACCCCCCGCCCGATTTTTT‑‑CCCCAGATA‑CC‑GAGATGCCG‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATc                                                                                 <  1:632748/248‑1 (MQ=255)
                                                                                                                                                                               ccagccagCAATTGGCGGGTTTGCTCGCGTTCCG‑CCGCCCGTGC‑TTTA‑ACCGTA‑CCCAGGC‑GGGCAGT‑AACCTCCGCCCGATTCTTT‑‑CCCCAGATA‑TC‑GAGATGCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGAc                                                                          >  1:815811/1‑250 (MQ=255)
                                                                                                                                                                                           tGGCGGTTTTGCTCGCTTCCCC‑CCGCCCTTCT‑TTTA‑CCCGTC‑CCCAGGC‑GGGCAGT‑ACCCCCCCCCCGATTCTTT‑‑CCCCAGATA‑TC‑GAGATCCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTg                                                               <  1:159452/249‑1 (MQ=255)
                                                                                                                                                                                              ggggTTTGCGCGCTTCCCC‑CCCCCGGTGC‑TTTA‑CCCGCA‑CCCGGGC‑GGGCAGA‑ACCCCCCCCCCGATT‑TTT‑‑CCCCCAAAA‑TCGGAGATGCCC‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCaa                                                           <  2:133394/249‑1 (MQ=255)
                                                                                                                                                                                               gggTTTGCTCGCGTTCCG‑CCGCCCGTGC‑TTTA‑ACCGTA‑CCCAGGC‑GGGCAGT‑AACCTCCGCCCGATTCTTT‑‑CCCCAGATA‑TC‑GAGATGCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAAc                                                          >  1:856115/1‑250 (MQ=255)
                                                                                                                                                                                                          ccctccccccccccGTCT‑TTTA‑CCCGCC‑CCCGGGC‑GGGCAGT‑ACCCCCCCCCCGTTTCTTC‑‑CCCCATATA‑TC‑GAGTTCCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCg                                                  <  1:286922/237‑1 (MQ=255)
                                                                                                                                                                                                              ccg‑ccgCCCGT‑C‑TTTAGAGCGTC‑CCCGGGG‑GGGCAAC‑AACCTCCGCCCGATTGTTA‑‑CCCCAGATA‑TG‑GAGATGCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGc                                           <  1:131148/250‑1 (MQ=255)
                                                                                                                                                                                                                  ccgccCTTGT‑TTTA‑CCCGTC‑CCCAGGC‑GGCAGGT‑ACCCCCCCCCCGATTCTTC‑‑CCCAAGATA‑TG‑GGGTTCCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTc                                        <  1:497694/250‑1 (MQ=255)
                                                                                                                                                                                                                    gccCGTGC‑TTTA‑ACCGTA‑CCCAGGC‑GGGCAGT‑AACCTCCGCCCGATTCTTT‑‑CCCCAGATA‑TC‑GAGATGCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCag                                      >  2:811006/1‑250 (MQ=255)
                                                                                                                                                                                                                               tA‑ACCGTA‑CCCAGGC‑GGGCAGT‑AACCTCCGCCCGATTCTTT‑‑CCCCAGATA‑TC‑GAGATGCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAAt                            >  1:35668/1‑250 (MQ=255)
                                                                                                                                                                                                                                ttaCCGTACCCCGGGC‑GGGAAAT‑ACCCCCCGCCCGTTTCTTC‑‑CCCCAGAAA‑TG‑GGGATGCAA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGa                              <  1:885920/247‑1 (MQ=255)
                                                                                                                                                                                                                                a‑aCCGTA‑CCCAGGC‑GGGCAGT‑AACCTCCGCCCGATTCTTT‑‑CCCCAGATA‑TC‑GAGATGCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATg                           >  1:563461/1‑250 (MQ=255)
                                                                                                                                                                                                                                  cccGCA‑CCCGGGC‑GGGCATT‑ACCCCCCGCCCGTTTCTTC‑‑CCCCAGATA‑CC‑GAGATGCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATgc                          <  1:951171/249‑1 (MQ=255)
                                                                                                                                                                                                                                      tA‑CCCAGGC‑GGGCAGT‑AACCTCCGCCCGATTCTTT‑‑CCCCAGATA‑TC‑GAGATGCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTg                      >  1:975570/1‑250 (MQ=255)
                                                                                                                                                                                                                                          ccAGGC‑GGGCAGT‑AACCTCCGCCCGATTCTTT‑‑CCCCAGATA‑TC‑GAGATGCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGtt                    >  2:409728/1‑249 (MQ=255)
                                                                                                                                                                                                                                            aGGC‑GGGCATT‑ACCCTCCGCCCGATTCTTC‑‑CCCCAGATA‑CC‑GAGATGCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAg                 <  1:769255/250‑1 (MQ=255)
                                                                                                                                                                                                                                               c‑GGGCAGT‑AACCTCCGCCCGATTCTTT‑‑CCCCAGATA‑TC‑GAGATGCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCa              >  2:2432/1‑250 (MQ=255)
                                                                                                                                                                                                                                                   gCAGT‑ACCCCCCGCCGGATTCTTC‑‑CCCCAGATA‑TC‑GAGATGCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCt           <  1:440119/250‑1 (MQ=255)
                                                                                                                                                                                                                                                             tCCGCCCGATTCTTT‑‑CCCCAGATA‑TC‑GAGATGCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCg  >  2:383693/1‑250 (MQ=255)
                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                  
CCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCG‑CCGCCCGTGC‑TTTA‑ACCGTA‑CCCAGGC‑GGGCAGT‑AACCTCCGCCCGATTCTTT‑‑CCCCAGATA‑TC‑GAGATGCCA‑GCCCG‑CCGTTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTCGTACCTG‑CGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACA‑TTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCCG  >  NZ_CP009273/2221507‑2222003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑