| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,372,447 | 0 | C | T | 33.3% | 63.8 / 16.4 | 55 | E98K (GAA→AAA) | menD | 2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylic‑acid synthase |
| Reads supporting (aligned to +/- strand): ref base C (9/27); new base T (17/1); total (27/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.05e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAT‑CA‑G‑TTTTTC‑TCCGGTTAACCCGGCTTCAA‑TCAGTGCCGGATAGAG‑ATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTC > NZ_CP009273/2372208‑2372693 | ttCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTTAGGG‑GAT‑TA‑G‑GTTTTCTTCCGGtcac > 2:29122/1‑247 (MQ=255) tCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAT‑CA‑G‑TTTTTC‑TCCGGTTAAc < 1:139938/249‑1 (MQ=255) tCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGGTACGAG‑GAT‑AC‑G‑ATTTTT‑TCCgggctacc > 1:329345/1‑244 (MQ=255) ccATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGGCAATTAGCTCCGGCGGGCGATCGGC‑GGGGAGAAG‑AAA‑CA‑G‑GTTTTT‑TTCGGGTGACCCgg > 2:110096/1‑250 (MQ=255) cATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGGTTAGAG‑AAA‑CA‑G‑TTTTTT‑TCCGGTTGACCCGGc > 2:368585/1‑250 (MQ=255) aGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAT‑CA‑G‑TTTTTC‑TCCGGTTAACCCGGCTTCaa < 2:2636/250‑1 (MQ=255) aaaCGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTTAGAG‑GAT‑CA‑G‑GTTTTT‑TCCGGGTAACCCGGCTTCCA‑TCacgggcggg > 2:623273/1‑242 (MQ=255) aaaCGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGGTTAGAG‑GATAAA‑G‑TTTTTT‑CCCGGTTAACCC‑GCTTCCA‑TCACTGCCggg > 1:775148/1‑249 (MQ=255) cAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGGTAAGAG‑AAA‑CAGG‑TTTTTT‑TCCGCGAAACCC‑GCCTCAA‑TCAGTGCCgggatgggat > 2:182521/1‑242 (MQ=255) gTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAT‑CA‑G‑TTTTTC‑TCCGGTTAACCCGGCTTCAA‑TCAGTGCCGGATAGAG‑Attt < 2:264391/250‑1 (MQ=255) gATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAT‑CC‑G‑GTTTTC‑TCCGGGTAACCCGGCCTCAA‑AAAAGGCCGGGTAGAG‑ATTTGcc > 1:985636/1‑250 (MQ=255) gATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAT‑CA‑G‑TTTTTC‑TCCGGTTAACCCGGCTTCAA‑TCAGTGCCGGATAGAG‑ATTTGc < 1:68957/249‑1 (MQ=255) atatGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGGTTAGGG‑GAT‑AA‑G‑GTTTTT‑TCCGGGTTACCCGGCTTCAA‑TCCGTACCGGATAGAG‑ATTTGTCa > 2:133268/1‑250 (MQ=255) aCTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTTAGAG‑AAT‑CA‑G‑TTTTTT‑TTCCGTTTACCCGGCTTCCA‑TCCGAGCCGGGTAGAG‑ATTTTccccccccc > 1:768622/1‑249 (MQ=255) cccccGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAT‑CA‑G‑TTTTTT‑TCCGGTTTACCCCGCTTCAA‑TCCGTGCCCGGTAGAG‑ATTTGTCCCCCCCGTGcc > 1:648626/1‑250 (MQ=255) cccccGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAT‑CA‑G‑TTTTTC‑TCCGGTTAACCCGGCTTCAA‑TCAGTGCCGGATAGAG‑ATTTGCCACCGCCGTGc < 1:538598/249‑1 (MQ=255) ccGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGGTAAGAG‑AAA‑CAGG‑TTTTTT‑TCCGGTAAACCGGCCTCCAC‑ACAGGGCCGGGAAAAG‑ATTTGcacccccccgccc > 2:857089/1‑236 (MQ=255) ctcaggAATCGGACCGAGAGCGTGGTCGATGGTGGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCGGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAT‑CA‑G‑TTTTTC‑TCCGGTTAACCCGGCTTCAA‑TCAGTGCCGGATAGAG‑ATTTGCCACCGCCGTGCCGGAg < 2:514155/245‑1 (MQ=255) cATGAAGCGTACCGAGAGCGTGGCCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAT‑CA‑G‑GTTTTT‑TTCGGGTAACCCCGCTTTAACTCAGGCCCGGATAG‑G‑ATTTTCCACCGCCGCGCCGGAGGt > 1:415885/1‑250 (MQ=255) aaGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAT‑CA‑G‑TTTTTC‑TTCGGGTTAACCGGCCTCAA‑TCAGTGCCGGGTAGAG‑ATTTTCCACCCCCCCGCCGGGGGGcac > 1:55456/1‑249 (MQ=255) gCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGCTTAGAC‑GAC‑CA‑G‑GTTTTT‑TGCGGGTCAATCGGCCTCAC‑CCCGTGCTGGCGAAAGAATTAGCGACCCCCGCGCCGAGGGTCACaa > 2:927007/1‑250 (MQ=255) tACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAT‑CA‑G‑TTTTTT‑TTCGGTTAAACCCGGTTCAA‑TCAGTGGCGGGTAGAG‑ATTTGCCACCGCCGGGCCGGAGGTCACAATCAc > 1:579970/1‑250 (MQ=255) aCCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAT‑CA‑G‑TTTTTC‑TCCGGGTAATCCCGCGTCAA‑TCAGTGCCGGATAGAG‑AATTGCCACCGCCGTGGCGGAGGGCTCAATCAcc > 1:613224/1‑250 (MQ=255) cGATGGTAGAAACCAGCCAACGTGCGGGGATATCCGGGGCCGGGCGCGGCAATGAAATACTGTGGGGGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCCCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAT‑CA‑G‑TTTTTC‑TCCGGTTAACCCGGCTTCAA‑TCAGTGCCGGATAGAG‑ATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCtgctt < 2:922734/250‑1 (MQ=255) cGATGGTAGAAACCAGCCAACGTGCGGGGATACCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGGGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAT‑CA‑G‑TTTTTC‑TCCGGTTAACCCGGCTTCAA‑TCAGTGCCGGATAGAG‑ATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCtgctt < 2:423970/250‑1 (MQ=255) gAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAT‑CA‑G‑TTTTTC‑TCCGGTTAACCCGGCTTCAA‑TCAGTGCCGGATAGAG‑AATTTCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCCGCTGCTTGCTGACtt > 1:353010/1‑250 (MQ=255) aaCCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGGTTAGAGAAAA‑CA‑G‑TTTTT‑‑TCCGGGTAAACCGGCTTCCA‑TCAGTGCCGGGTAGAG‑AATTGTCACCCCCGCGCCGGAGGGCACAAACACCCCCACCcgccgcgttcgtgctttt > 2:128454/1‑238 (MQ=255) cAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGGTTAAGA‑‑AAA‑CA‑GTTTTTTT‑TCGGGTTAACCCGCCTCCAT‑TACGGGCGGGTAAAAG‑ATTTTCC‑CCCCCCCGCCCGGGGGCCCAAACCCCCCCCCCCGCTGCTTTCTGACTTTCGcc > 2:679084/1‑250 (MQ=255) cAGCAAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGGGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAT‑CA‑G‑TTTTTC‑TCCGGTTAACCCGGCTTCAA‑TCAGTGCCGGATAGAG‑ATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGcc < 1:585213/250‑1 (MQ=255) gCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAT‑CA‑G‑TTTTTC‑TCCGGTTAACCCGGCTTCAA‑TCAGTGCCGGATAGAG‑ATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAg < 1:150983/250‑1 (MQ=255) tgcggtagactgcTGGGTCGGCCGCGCCATTGAATCACTGTGCGTGGTGTGGACGGCCACCATCCCCGCCTGTGTAAGCCCCTTTTCCCCCCCGCACTGATTAAGCCCGGCCGCGCGCTCGGC‑GGTTAAGAG‑AAC‑CA‑G‑TGTCCC‑CCCGCTTACCCCGCCTTAAT‑TCAGTGCCGTATAGAC‑TTTTCCCACCGCCGTGCCGGAGGTCACAATCACCTCCACCTGCTGCTTGCTGACTTTCGCCAGCCCCAgc < 2:531401/238‑1 (MQ=255) atatCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAT‑CA‑G‑TTTTTC‑TCCGGTTAACCCGGCTTCAA‑TCAGTGCCGGATAGAG‑ATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGAt < 1:533009/250‑1 (MQ=255) cccTGGGTCGGGCGCGGCAATGAAATCCTGGGCGTGGGGTGGGAGGCGAACATCCCCGGCTGGGGAATTGCCTGATTCGCGCCGCAGTCAATTAGCCCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAT‑CA‑G‑TTTTTC‑CCCGGTTACCCCGGCTTCAA‑TCAGTGCCGGATAGAG‑ATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGcc < 2:55456/249‑1 (MQ=255) gcgGAAATGAAATACGGTGCGGGGGGTGAGAGGCGAACTTCCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCCCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAT‑CA‑G‑TTTTTC‑TCCGGTTAACCCGGCTTCAA‑TCAGTGCCGGATAGAG‑TTTTCCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTc < 2:209507/250‑1 (MQ=255) aaTACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTAAAAG‑AAT‑CA‑G‑GTTTTT‑TCCGGGTTAACCGGCTTCAA‑ACAGTGCCGGGAAGAG‑ATTTTCCACCCCCCTGCCGGGGGGCACAATCACCGCCCCCCGCCGCTTTCTGACTTTTTCCCGCCCCAGCGCCCGATGCCCCCACCCACGCCCCTCGGAATgg > 1:95392/1‑250 (MQ=255) ggggggggggTGGGGGGCCAAATTCCCCGGCTGCGGATTTGCATGATTCGCGCCGCAGTCAATTACCCCCGGCGGGCGATCGGC‑GTTTAAGAG‑AAT‑CA‑T‑TTTTTC‑TCCGGTGACCCCGGCTTCAA‑CCAGCGCCGGATAGAG‑TTTGGCCCCCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGgtgt < 2:430050/242‑1 (MQ=255) ggTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAA‑CA‑G‑TTTTTT‑TCCCGGTTACCCGGCCTCAA‑TCAAGGCCGGATAAAG‑ATTTGCCACCGCCGGGCCGGGGGTCACAAACACCGCCACCCGCACGCTGCCGTCCTTCGCCCGCCCCCACACCCGACAGCCCCACCCCCGCCCATCACATTGTGGGTGGtgaatgt > 2:692053/1‑248 (MQ=255) agagGGGAACTTCCCCGGCGGGCGAATGCCCTGATACCCGCCGCAGCCAATTAGCTCCGGCGGGCGATCGGC‑GGTAAAGAG‑AAT‑CA‑T‑TTTTTC‑CCCGGTTAACCCGGCTTAAA‑CCAGCGCCGGATAGAG‑ATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCg < 2:46030/250‑1 (MQ=255) agagGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAT‑CA‑G‑TTTTTC‑TCCGGTTAACCCGGCTTCAA‑TCAGTGCCGGATAGAG‑ATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCg < 1:49550/250‑1 (MQ=255) gCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAT‑CA‑G‑TTTTTC‑TCCGGTTAACCCGGCTTCAA‑TCAGTGCCGGATAGAG‑ATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAAt < 1:951377/250‑1 (MQ=255) gCGAACATTCCCGGCGGGCGAATTGCCTGATTCGCGCCGCAGTAATTTAGCCCCGGCGGGGGATCGGC‑GGTTAAGAG‑AAT‑CA‑T‑TTTTTC‑CCCGGTTAACCCGGCTTCAA‑TCAGTGCCGGATAGAG‑ATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAAt < 2:628415/250‑1 (MQ=255) gAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAT‑CA‑G‑TTTTTC‑TCCGGTTAACCCGGCTTCAA‑TCAGTGCCGGATAGAG‑ATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATtc < 1:818857/250‑1 (MQ=255) ccGGCGGGCAAATGGCCGGATCGGCCCGGAAGTCAATTAGTTCGGGCGGGCGATCGGG‑GGTTAAAGA‑AAT‑CA‑G‑TTTTTT‑CCCGGTTACCCCGGCTTCAA‑TCAGGGCCGGATAGAG‑ATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTccgccgc < 2:833144/250‑1 (MQ=255) cGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAT‑CA‑G‑TTTTTC‑TCCGGTTAACCCGGCTTCAA‑TCAGTGCCGGATAGAG‑ATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTccgccgcc < 1:873607/250‑1 (MQ=255) gCGAATTGCCTGTTTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATGGGC‑GGTTAAGAG‑AAT‑CA‑G‑TTTTTC‑TCCGGTTAACCCGGCTTCAA‑TCAGTGCCGGATAGAG‑ATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTa < 1:441330/248‑1 (MQ=255) aTTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAT‑CA‑G‑TTTTTT‑TCCGGTTAACCCGGCTTCAA‑TCAGTGCCGGATAGAG‑ATTTGCCACCCCCGTGCCGGAGGTCACAATCACCCCCACCGGCTGCTTGCTGACTTTCTCCAGCCCCCGCGCCCGATGCCCCCACCCACGCTCATCCAAATGGGGGTGGGGGATGAATGCGGAATTCTCCGCCGCCGCTCACGtt > 1:359845/1‑249 (MQ=255) gcccgtcggaaaTTCGCTCCGGGGGGCGATGGGG‑GTTAAAGAA‑AAT‑CA‑G‑TTTTCC‑CCCGGTAACCCCGGCTTCAA‑CCAGGCCCGGATAGAG‑ATTTGCCCCCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAAcg < 2:709506/240‑1 (MQ=255) ggTTAAGAG‑AAT‑CA‑G‑TTTTTC‑TCCGGTTAACCCGGCTTCAA‑TCAGTGCCGGATAGAG‑ATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCg > 2:559891/1‑250 (MQ=255) ggTAAAGAG‑AAT‑CA‑G‑TTTTTC‑CCGGGTTACCCGGGCTCCAA‑TCAGGGCCGGAAAGAG‑ATTGGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCg < 1:522985/250‑1 (MQ=255) agag‑AAT‑CA‑G‑TTTTTC‑CCCGGTTACCCCGGCTTCAA‑TCAGTGCCGGATAGAG‑ATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGAc < 1:198021/249‑1 (MQ=255) g‑aAT‑CA‑G‑TTTTTC‑TCCGGTTAACCCGGCTTCAA‑TCAGTGCCGGATAGAG‑ATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGt > 2:520392/1‑250 (MQ=255) aaT‑CA‑G‑TTTTTC‑CCCGTTTAACCCGGCTGCAA‑TCAGTGCCGGATAGAG‑ATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGtt < 1:166870/250‑1 (MQ=255) tttttC‑TCCGGTTAACCCGGCTTCAA‑TCAGTGCCGGATAGAG‑ATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCt > 1:392932/1‑250 (MQ=255) tttttC‑CCCGTTAAACCCGGCTTCAA‑TCAGTGCCGGAGAGAG‑TTTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCt < 1:663993/250‑1 (MQ=255) ttttC‑TCCGGTTAACCCGGCTTCAA‑TCAGTGCCGGATAGAG‑ATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCtt > 2:945387/1‑250 (MQ=255) ttcc‑tcCGGTTAACCGGGCTTCAA‑TCAGTGCGGGATAGAG‑ATTTCCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCtt < 2:950127/246‑1 (MQ=255) c‑tcCGGTTAACCCGGCTTCAA‑TCAGTGCCGGATAGAG‑ATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTc > 1:310282/1‑247 (MQ=255) | TTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGC‑GGTTAAGAG‑AAT‑CA‑G‑TTTTTC‑TCCGGTTAACCCGGCTTCAA‑TCAGTGCCGGATAGAG‑ATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTAATGCTTC > NZ_CP009273/2372208‑2372693 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |