| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,428,361 | 0 | T | G | 36.9% | 109.0 / 17.9 | 65 | Y252S (TAC→TCC) | truA | tRNA pseudouridine(38‑40) synthase TruA |
| Reads supporting (aligned to +/- strand): ref base T (9/32); new base G (24/0); total (33/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.24e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAATTAAGAACAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGTTCGGGA > NZ_CP009273/2428112‑2428605 | aaaTTAAGAACAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTACCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGt < 2:141347/250‑1 (MQ=255) aCAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGTAGATCATAACGGCGCGGGGAATAC‑Accc > 2:239568/1‑249 (MQ=255) tgaaTAAACCCAGTCGCCGAACTCCGCGACCAGATCTGCCAGATGCACGTCAATGGGCAGGATAAAAGCGATGAAGAAATAAATCAGGTCCGAATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTTCGTTGGCTACAGCCTGAAATGTGCCGAGCATTATTCTAGCTAGAACACCAAAGATAGCGGGCCGAGTGGCGG‑TGTTGGAAGGTCATACCGGTCAGGGTAATCC‑GCCGCGGCCAg < 2:473084/247‑1 (MQ=255) aTAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑CTTTGGGAGATCATACCCGTCAGGGGAATCCAACcgcgccagca > 2:136586/1‑243 (MQ=255) tAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑GTTTTGGAGATCATACCGGTTACGGTAAACc‑ccccccgccaccta > 2:380002/1‑238 (MQ=255) aaaCCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGTGAGATAATAACCGTCAGGGGGACTC‑ACCGCGACCacaata > 1:555294/1‑244 (MQ=255) cAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGATCATACCGGCCAGGGTAAACC‑CACGCGACCAGATACAggcac > 2:933149/1‑246 (MQ=255) cGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCATACCCGTCCGGGGAATCC‑ACCCCGACCAGATACAGCCCCTccc > 1:355687/1‑249 (MQ=255) cGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCtt < 2:799991/250‑1 (MQ=255) cgcgACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGcc < 1:977205/248‑1 (MQ=255) gcgACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑tt < 2:138732/250‑1 (MQ=255) ctGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGTAGGATTAACCCGGCATGGGTAACCC‑CCCCCG‑CCACGGACAAGCCTTCCCCCTTTTCCG‑TTGCTgcctccc > 2:397288/1‑249 (MQ=255) tGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGATTTTGCCG‑TTGCTgccgcc < 1:590289/248‑1 (MQ=255) cAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGATGATCCTACCGGGCCGGGTAATCC‑ACCCCGACCCGATACAGCCCCTTCGCCTTTTCCg‑ctgcttgcccccccgt > 2:369135/1‑239 (MQ=255) gTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGGTCATACCCGGCAGGGGAATCC‑ACCCCGCCCCGATACAACCCCTCCCCCTTTTCCC‑TTGCGTCCCCCCCCGCTCTGTCCtt > 2:714516/1‑250 (MQ=255) aTGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTgcc < 1:918273/250‑1 (MQ=255) gCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGATCCAACCGGTCAGGGGGATCC‑ACCGCGGCCAGATACAGCCCTTCCGCCTTTTTCG‑GTGCTGCCGCCAACGTTCTGTCCCTTgccgc > 1:814246/1‑248 (MQ=255) aGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGc < 2:928509/250‑1 (MQ=255) aGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGc < 2:658566/250‑1 (MQ=255) ggATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGATCATAACCGGCAGGGGAATCC‑ACCCCGACCAGATAAAACCCCTCCCCTTTTGCCG‑GTGCTGCCGCCCGCGCTCTGTCCTTTGCCCCCacc > 1:572665/1‑247 (MQ=255) gATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCaa < 2:284216/250‑1 (MQ=255) cGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATTATACCGGGCAGGGGAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTTCCG‑TTGCTGCCGCCCGCGACCTGTCCTTTGCCCCCCACCACTCTGCtt > 2:751491/1‑249 (MQ=255) ataaatCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATACCGGTCAGGGGAATCC‑ACCGCGACCAGAAACAACCCTTCCGCTTTTGCCG‑TTGCTGCCCCCAGCGGTCTGTCCCTTTCCGCCAGCAACTCTGCTATCCAGCtctc > 1:707737/1‑250 (MQ=255) aatCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCgg < 2:163739/250‑1 (MQ=255) aatCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGGAATCC‑ACCGCGACCAGATACAGCCCTTCCCCTTTTTCCG‑TTGCTGCCGCCCGCGGTCTTTCCTTTTCCCCCCGCAACTCTGCTATCCAGCTCTCCgg > 1:668641/1‑250 (MQ=255) tCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGGCAGGGGAATCC‑ACCGCGACCAGATACAACCCCTCCGCTTTTTCCG‑TTGCTGCCGCCCGCGGTTTGTCCCTTTCCGCCCGCAACTCTTCTATCCCGCCCTCCGGCTGGGtg > 1:444901/1‑250 (MQ=255) cATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTtgt < 2:572410/249‑1 (MQ=255) tatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTTGAGATCATACCGGGCAGGGGAATCC‑ACCGCCACCCGATACAGACCCTCCGCTTTTGCCG‑TTGCTTCCCCCCGCGGTCTGTCCTTTTCCGCCCCCAACTCTGCTTTTCAGCTCTCCGGCTGGTTGTggg > 2:859707/1‑250 (MQ=255) tatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATAACGGTCCGGGGAATCC‑ACCCCGACCAGATACAACCCTTCCCCCTTTTCCG‑TTTCTGCCCCCACCGGTCTGTCCCTTGCCCCCCGCCACTCTGCTTTCCACCTCTTCCGCCGGTTGTggg > 2:845436/1‑250 (MQ=255) tttGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGATCATAACGGGCCGGGGAATCC‑ACCGCGACCAGATAAAGCCCTTCCCCTTTTTCCG‑TTTCTGCCGCCCGCGGTCTGTTCTTTTCCGCCCGCAACTCTGCTATCCCGCTCTCCGGCTGGTTGTtggga > 1:81025/1‑248 (MQ=255) aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑GTTTTGGAGATCAAACCGGTCAGGGGAATAC‑AACCCGGCCAGGTACAACCCCTCCCCTTTTTCCGTTTGTTCCCCCCACCGTCTTTTCCTTTCCCCCCGGCCATCTTCCTGTCC‑GCTCTCCCGCCTGTTGTGGGGAcccccct > 2:971823/1‑245 (MQ=255) ttGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCATAACGGTCAGGGGAATCC‑ACCCCGACCAGATACAGCCCCTCCCCTTTTTCCG‑TTGCTGCCCCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCCACTTccct > 1:403370/1‑247 (MQ=255) tGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCATACCGGTCAGGGGAATCC‑ACCCCGACCCGAGACAACCCCTCCCCTTTTTCCG‑GTTCTGCCGCCCGCGGTTCGTGCCTTTTCGCCCCCCACCCTTCTCTCCACCTCTCCCGCGTGTTTTGGGCTCCGACCTCCATcc > 2:389978/1‑249 (MQ=255) gTACTCGTCATCTTTCAGGCTGTAACTACGGTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATc < 1:659881/248‑1 (MQ=255) aCTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATACCCGGCAGGGGAATCC‑CCCCCGACCCGATACAGCCCTTCCCCCTTTTCCC‑TTGCTTCCGCCCCCGGTCTGTCCTTTTTCGCCCCCAACTCTCCTATCCACCCCCCCCGCTGGTTGTGGGGACCCCCCTCCACCAGGc > 2:720124/1‑250 (MQ=255) cTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATACCGGGCAGGGGAATCC‑ACCCCGACCAGATAAAACCCTTCCCCCTTTGTCG‑TTGCTGCCGCCCGCCGTCTTTCCTTTTTCGCCCGCCAATCTGCTCTCCCGCTCCCCGGCTGGTTGTGGGCGCCGCCTTCCATCCGGACGCCGAcca > 2:104638/1‑248 (MQ=255) ggCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTcc < 1:101626/250‑1 (MQ=255) tGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGAACCTACCCGTCAGGGGAATCC‑ACCCCCACCCAAAAAAACCCTTCCGCTTTTTCCG‑TTGTTGCCGCCCGCGGTCTGTTCTTTTCCCCCCGCCACTCTTCTATCCAGCTCTCCCGCCGGTTGGTGGGACCCACCTCCCTCCTGCTGCCGGCCAAATTCCTGa > 2:508064/1‑250 (MQ=255) tGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGAACATACCCGTCAGGGGAATCC‑ACCCCGGCCCGATACAGCCCCTCCGCTTTTTCCC‑TTTCTTCCCCCCCCGTTCTGTCCTTTTTCCCCCCCACCCCTGCTATCCCGCTCTCCCGCTGGGTGTGGGGACCGCCTTCCCTCCGGCTGCCGCCCAAATTTCTgt > 2:504670/1‑249 (MQ=255) aCTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCa < 1:47342/249‑1 (MQ=255) ggCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGt < 1:296162/249‑1 (MQ=255) tGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCATACCGGGCAGGGTGATTC‑CCCGCGACCCGATACAAGCCTTTCGCTTTTTTCG‑TTGCTGCCCCCCGCGGTCTGTCCTTTTCCGCCCGCAACTCTGCTCTTCCGCACTCCCGCTGGTTGTGGGCACCGACTTCCCTCAGGCTGGCGAGCATATTACTGTCAATCTGCTGTATACAAGCaca > 2:257902/1‑248 (MQ=255) aaaTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCg < 2:690686/250‑1 (MQ=255) aaaTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATGGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCg < 2:533708/250‑1 (MQ=255) aTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCt < 1:991726/250‑1 (MQ=255) tGTTCCGAGCAGTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCtt < 2:579228/250‑1 (MQ=255) tCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCATAACCGTCAGGGGAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTTCTGCCGCCAGCGGTCTGTCCTTTTCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCCTCAGGCTGCCGACAATATTTCTGACCATAAGATTTACCAAAGCATTCGCtttta > 1:735552/1‑248 (MQ=255) ccGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTaa < 2:198936/249‑1 (MQ=255) gTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACcaca < 1:718501/248‑1 (MQ=255) ttAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATaa < 1:709933/250‑1 (MQ=255) ttAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATaa < 1:866987/250‑1 (MQ=255) ccGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGAcc < 2:286976/250‑1 (MQ=255) cGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGAcc < 1:942613/249‑1 (MQ=255) cGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGGAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTTCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCCGCAACTCTGCTATCCAGCTCTCCGGCTGGGTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTAACACCACATAAAGACCg > 1:473022/1‑250 (MQ=255) aGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCa < 2:960541/172‑1 (MQ=255) gAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCATACCCGTCAGGGGAATAC‑ACCCCGACCAGATACAGCCCCTCCCCTTTTGCCG‑TTGCTGCCCCCCGCGGTCTGTCCTTTTCCGCCCGCCACTCTCCTATCCCCCTCTCCGGCTGGGTGTGGGGGCCGACTTCCCTCAAGCTGCCGACCATATTCCTGACCATATAATGTACAAAAGCATTCGCTTTAATATCTACCCCCACATAAGAACCGTGAcc > 2:524927/1‑249 (MQ=255) aTTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCTACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAatat < 2:99411/250‑1 (MQ=255) aTTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAatat < 1:793315/250‑1 (MQ=255) ttGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATg > 1:868251/1‑250 (MQ=255) tGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGtt < 2:815873/249‑1 (MQ=255) gtgactCATACCGGTCAGGGTAAGCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTgcg < 2:785555/245‑1 (MQ=255) gAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTgcg > 2:12493/1‑250 (MQ=255) tCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACg < 1:817455/250‑1 (MQ=255) ccGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGt < 1:760910/249‑1 (MQ=255) tCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGTTCgg > 2:562488/1‑249 (MQ=255) cAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACTGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGTTCGGGa > 1:982426/1‑250 (MQ=255) | AAATTAAGAACAAAATGGCATAAACCCAGACGCCGTACTCCGCGACCAGCTCTGCCAGATGCACGTCAATGTGCAGGATAAAATCGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCC‑ACCGCGACCAGATACAGCCCTTCCGCTTTTGCCG‑TTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATATGATGTACAAAAGCATTCGCTTTAATATCTACCACCACATAAGGACCGTGACGCGTGACATTAATATGCATAACGTTGCGCCACGGGGTTCGGGA > NZ_CP009273/2428112‑2428605 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |