| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,036,390 | 0 | A | G | 44.4% | 91.0 / 21.5 | 72 | N206D (AAT→GAT) | srkA | stress response kinase SrkA |
| Reads supporting (aligned to +/- strand): ref base A (19/21); new base G (32/0); total (51/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.82e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCC > NZ_CP009273/4036142‑4036638 | aggCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATCCCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGaa < 2:692770/248‑1 (MQ=255) ggTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTTACCGTTCTG‑CGGCTACATTGAGACTGCCCCCCCCGGGATAtt > 1:190391/1‑250 (MQ=255) ggCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAG‑ATTTTCCCGGTCTG‑CGGCCACATGGAGACTGGCAAGTCGGGGAAATtatcct > 1:1006831/1‑248 (MQ=255) gCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTgg < 2:498080/250‑1 (MQ=255) cGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTTACCGTTCTG‑CGGCTACATGGAGACTGCCCCGCCCGGGATATTTTCTggg > 1:273681/1‑249 (MQ=255) ttAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTTACCGTTCTG‑CGGCGACATGGGGGCTGCCACCCCGGGGAAATTTTCTGGCGGGAT‑g > 1:865224/1‑250 (MQ=255) gTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAG‑ATTTTACCCTTCTG‑CGGCTACATGGAGGCTGCCACGCCGGGGATATTCTCTGGCGCGAT‑GGTCCCAt > 1:182141/1‑250 (MQ=255) tGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAG‑ATTTTACCGTTCTG‑CGGCTACATGGGGACTGCCACGCCGGGGATATTTTCTGGCGCGGT‑GGTCCCAtg‑tt > 1:908114/1‑250 (MQ=255) cACCAGACGGGGCGAAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATTTCTCATTGGGCCACGCAAGCTTTTTGAGGACGGTACACGGATCCCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAAtg‑tttg < 2:570853/250‑1 (MQ=255) aGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAG‑ATTTTACCGGTCTT‑CGGCTACCTTGAGACTGCCCCGCCGGGGAAATTCTCTGGCGCGGT‑GGTCCAATG‑TTTTTTGa > 1:830454/1‑250 (MQ=255) gACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGGATATTCTCTTGCGCGAT‑GGTCCAATG‑TTTGTTGAt > 1:538744/1‑250 (MQ=255) aCGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGGATATTATCTGGCGCTGT‑GGTGCACgg‑gttgtttgtt > 1:781161/1‑246 (MQ=255) gcgcAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAG‑ATTTTCACGGTCTGCCGCCTCCACGG‑GACTGGCCCGCCCGGGATAATTTCTTGGGCGGTGGGTCCATTG‑‑TTGTTTGTCT‑Ggat > 1:471873/1‑250 (MQ=255) cAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑AGTTCAACGGTCTG‑CCGCTACATTGAGACTGCCACGCCGGGGAAATTTTCCTGCGCGGA‑GGGCCAAAGTTTTGTTG‑TCT‑Gggtgat > 1:866837/1‑250 (MQ=255) cAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAG‑ATTTTACCGTTCTG‑CGGCTACATGGGGAACGCCCCGCCCGGAATATTTTCTGGCGCGCT‑GTTCTCCTA‑TTTTTTTTTCT‑TGGTGAT‑GCAc > 2:978643/1‑250 (MQ=255) aGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAA‑ATTTTTCCCGTCTG‑CGGGCACATGGAGAACGCCCCGCCGGGGATATTTTTCGGGGCGGT‑GGGCCACTA‑TTTGTTGATCT‑GTGTGAT‑Gcgcg > 2:705881/1‑250 (MQ=255) gCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGt < 1:153100/250‑1 (MQ=255) cTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTCACCGCTCTG‑CGGCTACATGGAGACTGCCACCCCGGGGATATTTTCTTGCGCGAA‑GGTCCCATG‑TTTTTTGATCT‑TGgtgat‑ggccgg > 1:167712/1‑244 (MQ=255) ttATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTTACCGTTCTG‑CGGCTACATGGGGAATGCCACGCCGGGGATATTTTCTGGGGCGGT‑GGGCCCATG‑TTTGTTTATCT‑GGGTGAT‑GCCCggtattg > 1:607323/1‑244 (MQ=255) tATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGGATATTTTCTTGCGCGGT‑GGTCCCATG‑TTTGTTTTTCT‑GGATGAT‑GCACGTAAT‑ggg > 1:64157/1‑249 (MQ=255) cATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTCACCGGTCTG‑CGGCCACATGGAGGATGCCACGCCGGGGATATTCTCTTGGGCGGT‑GGTCCAAAG‑TTTGTTGATCTGGGTTGAG‑GCACGAAAG‑GGTcag > 2:305961/1‑248 (MQ=255) aTCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTTACCGTTCTG‑CGGCTACATGGAGACTGCCCCGCCGGGGATATTCTCTGGCGCGAG‑GGTCCCATG‑TTTGTTGATCT‑TGGTGAT‑GCCCGTAAT‑GGTCCAGc > 1:767718/1‑250 (MQ=255) aTCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTTACCGTTCTG‑CGGCGACATGGGGACTGGCCCGCCGGGGATATTCTCTGGCGCGAT‑GGTGCCATG‑TTTGTTGGTCT‑GGATGAT‑GCACGTAAT‑GGTCCAGc > 2:778489/1‑250 (MQ=255) aTCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAG‑AATTTACCGTTCTG‑CGGCTACATGGGGACTGCCACGCCGGGGATATTTTCTGGCGCGAG‑GGTCCAATG‑TTTGTTGATCT‑GGGTGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGGtt > 2:19094/1‑250 (MQ=255) gAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTTACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGGATATTTTCTGGGGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGGTGAT‑GTAAGTAAT‑GGTCCAGCCGTTCAGGGTTTTTGGGTg > 1:563464/1‑250 (MQ=255) tcATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑GTTTTACCCGTCTT‑CGGCTACATGGAGAATGCCCCGCCCGGGATATTCTCTTGCGCGGT‑TGTTCCATT‑TTTTTTTGTCT‑TGATGAT‑GCACGGAAT‑GGTTCCGCCGTTCTGGTTTTGTGGGTGTTGT‑TCAATgg > 2:844060/1‑250 (MQ=255) tcATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑AATTCACCGGTCTG‑CGGCCACATGGAGACAGCCACGCCCGGAATTTTCTTTGGCGCGGT‑GGGTCCATG‑TTTTTTGGTCT‑TGATGAT‑GCACGTTAT‑GGGCCCGCCGTTCAGGATTTGGTGATGTTGG‑TTAAttg > 2:145568/1‑248 (MQ=255) gAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGAATTTCA‑CGTTTTG‑CGGCGACAAGGGGGAAGCCCCCGCGGGGATTTTTTCTGGCGCGCG‑GGGCCACTT‑TTTGGTGATCT‑TGATGGTGGCAC‑TTAT‑GTGTCAGCCCTTTTAGATTTGTGGGTTTTGCTTCAATGGGGa > 2:998713/1‑249 (MQ=255) gAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAG‑AATTCACCGTTCTG‑CGGCTACATGGAGGCTGCCACGCCGGGGGAATTCTTTGGCGGGGT‑GGGTCAATG‑TTTGTTGTTCT‑GGGTGGA‑GGCCGTAATGGGTCCGCCCGTT‑AGGATTTGTGGGTGGTTG‑TCAATGGGGAt > 2:803364/1‑249 (MQ=255) gAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTCACCGGTCTG‑CGGGTACATGGAGACTGCCACGCCGGGGATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTTATCT‑GGGTGAT‑GCACGTAAT‑GGGCCAGCCGTTCAGGGTTTGTGGATGTTGC‑TCAATgggggg > 1:1001680/1‑245 (MQ=255) ccACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTTACCGTTCTG‑CGGCCACATGGAGACTGCCACGCCGGGGATATTTTCTTGCGCGAT‑GGGCCAATG‑TTTGTTGATCT‑GGATGAT‑GCAAGTAAT‑GGTCCAGCCGTTCAGGGTTTGTGGGTGTTGC‑TCAATGGCGATAAAg > 1:10570/1‑250 (MQ=255) tGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAG‑GTTTTCCCGTTCTG‑CTGCTACCTTGAGACTGCCACGCCGGGGGTCTTTTCTTGTGCGGT‑GGTTCCATG‑TTTTTTTTTCT‑GGATTAT‑GGTAGTAAT‑TGTCCAGCCGTTCAGGATTTGTGGGTGTTGG‑TCTAGTGCGGTAAAACCGGACAGCTGATg > 2:839780/1‑250 (MQ=255) ggACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTTACCGGTCTG‑CGGCTACATGGAGACTGCCACGCCGGGGATATTTTCTTGCGCGAT‑GGGCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTGAT‑GGTCCAGCCGGTTGGGGGTTGTGGGTGTTGC‑TCAATGGCGAGAAAACCGCGCAGCgggggga > 2:451036/1‑244 (MQ=255) cacTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGAGGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAAc < 2:903716/250‑1 (MQ=255) cTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAG‑ATTTTACCGTTCTG‑CGGCTCCATGGGGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCCATG‑TTTTTTGGTCT‑GGGTGAT‑GCACGTAAT‑GGTCCAGCAGTTCCGGATTTGTGGATGTTGC‑TCAATGGCGATAAGGCCGAGCCGAGGAGGGAACTGGAGACta > 2:395651/1‑250 (MQ=255) cGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTTACCGTTCTT‑CGGCTACATGGAGACTGCCACGCCGGGAATATTTTCTGGCGCGGT‑GGTCCAATG‑TTTTTTGATCT‑TGGTGAT‑GCACGGAAT‑GGTCCAGCCGTTCAGGGTTTGTGGGTGTTGC‑TCAATGGCGGTAAAGCCGAGCAGCGGGTGCAACTTGGAAATATTATTTAAGCt > 1:841207/1‑250 (MQ=255) ggTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGGATATTTTCTGGCGCGAT‑GGGCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGGCCAGCCGTTCAGGATTTGTGGGTGTTGC‑TCAATGGCGATAAAACCGAGCAGCGGATGCAACTGGAAACTATTATTTAAGGttt > 1:688002/1‑249 (MQ=255) aGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATgaagaa < 2:352973/250‑1 (MQ=255) ccTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTCACCGGTCTG‑CGGCTACATGGAGACTTCCACGCCGGGGATATTTTCTGGCGCGGT‑GGTCCCATG‑TTTTTTGGTCT‑GGATGAT‑GCAAGGAAT‑TGTCCAGCCGTTCAGGGTTTGTGGGTGTTGG‑TCAATGGGGGATAAGCCGAGCCGCGGGGTGAAATGGAAACTATTATTTAAGGTTATGAAGAATTTAGCGAg > 2:753101/1‑250 (MQ=255) ccTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑AATTTACCGCTTTG‑CGGCTACAAGGGGACTGCCACGCCGGGGAAATTCTCTGGCGCGGT‑GGGCCCATG‑TTTTTTGGTCT‑GGGGGAG‑GCACGGAAT‑GGGCCCGCCGTGTAGGGTTTTTGGGTGGTGT‑TCAATGGGGGAAAAACCGAGGAGCGGGTGCGAATGGGAACATTTTTTGGAGCTTTTTAAGAATTTAACggg > 2:17156/1‑248 (MQ=255) gtGAAAGCGACAGATGAGCTACTTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGt < 2:730927/249‑1 (MQ=255) ggAAAGCGACAGAGGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGtt < 2:166104/249‑1 (MQ=255) aaGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTTCACGTTTTG‑CGGCCACATGGGGACTGCCCCGCCGGGAATATTTTCTGGCGCGAT‑GGGCCAATG‑TTTGTTGATCT‑GGGGGAT‑GCACGGAAT‑GGTCCAGCCCTTCAGGGTTTGTGGGTGTTGT‑TCAATGGCGATAAAACCGCGGAGAGGATGCAACCGGGAAATATTTTTGGAGATTATGAAGAAATTAGCGAGTTCgt > 2:755207/1‑249 (MQ=255) gATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTg < 1:784084/250‑1 (MQ=255) gtGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGa < 2:665436/249‑1 (MQ=255) aTGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTTACCGTTTTG‑CGGCTTCATGGAGGCTGCCCCGCCGGGGATATTTTCTGTCGCGGT‑GGTCCCATG‑TTTGTTTTTCT‑GGGTGAT‑GGACGGAAT‑GGTCCCGCCGGTCTGGGTTTGTGGGTGTTGC‑TCAATGGCGATAAAGACCAGCAGCGGGTGCAACTGGGCACTATTATTTAAGCTTATGAGAAAATTAGCGAGTTTGACACCGCTg > 2:414926/1‑249 (MQ=255) aTGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GTACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGa < 2:509948/250‑1 (MQ=255) cTGATGGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCg < 2:709976/250‑1 (MQ=255) tGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACt < 2:356114/249‑1 (MQ=255) ccgTTACAGCACACTGGCGGGAAG‑ATTTTACCGCTCTT‑CGGCTACATGGAGACTGCCACGCCGGGGATATTTTCTTGCGCGGT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑TGTCCCGCCGTTCAGGGTTTTTGGGTGTTTC‑TCCATGGCGATAAAACCGAGCAGCGGGTGCACCTGGGAACTATTATTTAAGCTTATGAAGAATTTATCGAGGTTGACACCCCTGAAAACGGACTGGttt > 2:614182/1‑248 (MQ=255) ccgTTACAGCACACTGGCGGGAAG‑ATTTCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGa < 1:917581/250‑1 (MQ=255) cgTTACAGCACACTGGCGGGAAG‑ATTTCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGaa < 1:458431/250‑1 (MQ=255) cgTTACAGCACACTGGCGGGAAG‑ATTTCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGaa < 1:277891/250‑1 (MQ=255) aCAGCACACTGGCGGGAAG‑ATTTCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCtt > 1:352258/1‑250 (MQ=255) acTGGCGGGAAG‑ATTTGACCGGTCTG‑CGGTTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACgcgc < 2:309537/250‑1 (MQ=255) cGGGAAG‑ATTTCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGc > 1:499775/1‑250 (MQ=255) gAAG‑ATTTCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGaa > 2:669350/1‑216 (MQ=255) gAAG‑ATTTCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGtt > 1:163328/1‑250 (MQ=255) aaG‑ATTTCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGaaa < 1:669350/216‑1 (MQ=255) tttCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGtt > 2:840008/1‑250 (MQ=255) gTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGTATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATc > 2:43642/1‑250 (MQ=255) ttCTG‑CGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCt > 1:658700/1‑250 (MQ=255) tCTG‑CGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTCTTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATAGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCtt > 2:912325/1‑250 (MQ=255) cGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTg < 2:830454/247‑1 (MQ=255) cATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTaa > 1:140729/1‑250 (MQ=255) aTGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAAt > 2:515732/1‑250 (MQ=255) aTGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAAt > 1:966142/1‑250 (MQ=255) agaCTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATgcgg < 1:131517/250‑1 (MQ=255) aCTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATgcggcg > 1:673544/1‑250 (MQ=255) gCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTg < 1:630970/250‑1 (MQ=255) cGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCt > 1:671352/1‑250 (MQ=255) ccGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGa > 2:645738/1‑250 (MQ=255) gAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATccc < 2:230081/250‑1 (MQ=255) | AAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAG‑ATTTCACCGTTCTG‑CGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATG‑TTTGTTGATCT‑GGATGAT‑GCACGTAAT‑GGTCCAGCCGTTCAGGATTTGTGGATGTTGC‑TCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGCTGATCCC > NZ_CP009273/4036142‑4036638 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |