| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 339,155 | 0 | A | G | 32.8% | 110.1 / 21.5 | 65 | G187G (GGA→GGG) | yahK | NADPH‑dependent aldehyde reductase YahK |
| Reads supporting (aligned to +/- strand): ref base A (5/38); new base G (20/1); total (26/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.07e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AACTACTGTGATCACATGACCGGCACCTATAACTCGCCGACGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCG > NZ_CP009273/338907‑339401 | aaCTACTGTGATCACATGACCGGCACCTATAACTCGCCGACGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGAc < 1:645453/250‑1 (MQ=255) tactGTGATCACATGACCGGCACCTATAACTCGCCGACGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCaaa > 1:66004/1‑248 (MQ=255) ctGTGATCACATGACCGGCACCTATAACTCGCCGACGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACatat < 1:359796/249‑1 (MQ=255) ctGTGATCACATGACCGGCACCTATAACTCGCCGACGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATg < 1:677125/250‑1 (MQ=255) ctGTGATCACATGACCGGCACCTATAACACGCCGACGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATg < 2:388904/250‑1 (MQ=255) cacaTGACCGGCACCTATAACTCGCCGACGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGCAAAAAAGTTGGCGGGGTCGGCATCGGCGGTGTGGGCGCTATggggggt > 2:520782/1‑246 (MQ=255) tGACCGGCACCTATAACTCGCCGACGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCt < 2:386664/250‑1 (MQ=255) tGACCGGCACCTATAACTCGCCGACGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCGTGGGCATATGGGGGTTAAGCt > 1:647788/1‑250 (MQ=255) ggCACCTATAACTCGCCGACGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGc‑cc < 1:504706/250‑1 (MQ=255) taACTCGCCGACGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGa < 1:513700/248‑1 (MQ=255) gCCGACGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGca < 1:175318/250‑1 (MQ=255) gCCGACGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGc < 1:430884/249‑1 (MQ=255) gcGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACAtg < 2:88290/249‑1 (MQ=255) aCGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACAtg < 1:100299/250‑1 (MQ=255) cGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGTTATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGGCGGCATCGGCGGTCCCGGGCCTATTGGGAGTAAGCTGGC‑CCCCGCGCGGGGGGGACAtgt > 2:159824/1‑250 (MQ=255) ccGGACGAACCGGGCCATACTCTGGGCGGCTACTCACACCAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATgtgg < 1:408923/250‑1 (MQ=255) cGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCGGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCCTGTGGGGGTTAAGGTTGG‑CCCCGCGCTGGGGGGGCATGTGGTGGcg > 2:175032/1‑249 (MQ=255) cGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCa < 2:150091/250‑1 (MQ=255) gAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCATATGGGGGTTAAGCTGGC‑CCACGCGATGGGGGGACATGtggtggggt > 1:254514/1‑247 (MQ=255) gAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGGCCGGGGGGAATGGGGGTTAAGGTGGG‑CCACCCGCTGGGGGGGCATGggggggggt > 2:669040/1‑247 (MQ=255) ccGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTa < 2:180174/250‑1 (MQ=255) cGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGGCTGGGGCATATGGGGGTTAAGCTGGG‑CCACGGGGGGGGGGGACATGGGGGGGGA‑ttttc > 1:41992/1‑248 (MQ=255) cATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACtt < 1:12388/250‑1 (MQ=255) gggCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGGCTGGGGCATATGGGGATTTAGCTGGG‑CCACGCGGTGGGGGGACATGGGGGGGGA‑TTTTACACTTCTggggg > 1:211312/1‑248 (MQ=255) gcggcTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGGCTGGGGCAGATGGGGGGTAAGCTGGG‑CCGCCCGATGGGGGCGCCCGTGGTGGGA‑TTTTCCACTTCTTAGGGaa > 2:520524/1‑249 (MQ=255) ggcTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTTTGGGGCACATGGGGATTAAGCCGGC‑CCACGCGATGGGGGCACACGTGGGGGCA‑TTTACCACTTCTGAGGCaaa > 1:46224/1‑248 (MQ=255) cTACGCACAACGGATCGTCGTTCATGATCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACTTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGTCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCTCGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAc‑gc < 1:740954/250‑1 (MQ=255) cacaACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGGCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGGCCGTGGGATTTGGGGGGTTAGCTGGCGCCAC‑CGCAGGGGGGACAATAGGTGGGATTTTCCCACTCTTGA‑GCAACAC‑GCGAAgt > 2:714647/1‑249 (MQ=255) aacaGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGc < 1:277524/250‑1 (MQ=255) cgtTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGGCTGGGGAATTTGGGGGTTAAGGTTGC‑CCCCGCGGTGGGGGGCCAAGTGGGGGGC‑TTTTTCACTTCTGTGGCAAAAA‑GCGGAAGGGCAAAAGacccg > 2:669365/1‑248 (MQ=255) gtTCATGGGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTgg < 2:509350/250‑1 (MQ=255) ttCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGGCTGGGGCATATGGGGGTTAAGCTGGG‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTTCCCCTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTggg > 1:273900/1‑250 (MQ=255) gAGCGATATGTTCTGCGTATTCGTCACCCGGAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGGGCGGAGATCCCCGCGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TGTACCACTGCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTGGGGGcc < 2:39059/249‑1 (MQ=255) cGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCATATGGGGGGTTAGATGGG‑CCCCCCGGGGGGGGGGCATGTGGGGGGA‑GTTTCCACTTCTGTGGGAAAACAGCACAGGGGAAAAAGCCCTggggccgggg > 2:657799/1‑244 (MQ=255) gATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTGGGGGCCGATGa < 1:300195/250‑1 (MQ=255) gATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTGGGGGCCGATGa < 2:551387/250‑1 (MQ=255) tCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAAt < 2:707411/250‑1 (MQ=255) ctcccGCAGGAGCAGCTGGCGGCGGTGGCTCCGTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTGGGGGCTGATGAAGTTGTTAACTCACGCAATg < 2:368024/248‑1 (MQ=255) ccGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCg < 1:188652/250‑1 (MQ=255) agcagcTGGCGGCGGTGGCTCCTTTGTTGGGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGAt < 2:480384/250‑1 (MQ=255) gcTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATggcg < 2:740256/250‑1 (MQ=255) cTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCATATGGGGGTTAAGCTGGC‑CCACGCGGTGGGGGCACATGTGGGGGGA‑TTTACCACTTCTGAGGCAAAAC‑GCGGAGCGGCAAAAGCCCCGGGGGGCGATGAAGGTGTTTACTCACGCAAAGCCGATGAGAGggcgg > 1:269723/1‑250 (MQ=255) tGGCTACTTTGTTGTGTGCAGGGATCACAACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGaa < 2:144391/250‑1 (MQ=255) cTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGtt < 1:450765/249‑1 (MQ=255) cTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGtt < 1:501453/249‑1 (MQ=255) ttgttgTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCATATGGGGGTTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGGTGTTAACTCACGCCATGCCGATGAGATGGCGGCTCATCTGAAGAGgttt > 1:286816/1‑248 (MQ=255) tgttgtGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTc < 1:676144/248‑1 (MQ=255) gtgtgCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCCTATGGGGGTTTAACTGGG‑CCACGCGCTGGGGGCGCATGTGGGGGCG‑TTTTCCACATTTGTGGCAAAAC‑GCGAAGGGGCAAAAACCCCCGGGGCCGAGGAAGTTGTTTAAACACGCGATGCCGATTGGAGGGGGGCGCACCTGAAGAGGTTTGAtttt > 2:701603/1‑249 (MQ=255) gaGGGAGCACCGCGTATTCGCGGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCATATGGGGATTAAGCTGGC‑CGACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTTAGGCAAAAC‑GCGAAGCTGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAAAGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCAtttt < 2:172869/249‑1 (MQ=255) aTCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCATATGGGGGTTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGGTTTCCTTTTTAATa > 1:653785/1‑250 (MQ=255) gTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCATTTTGAATACAGTAGCt < 1:670544/250‑1 (MQ=255) ttCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTgc < 2:384054/249‑1 (MQ=255) cgccgcTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGGTGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAACCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGGTTCGATTTCATTTTGAATACAGTAGCTGCGcc > 1:721787/1‑250 (MQ=255) tACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCATATGGGGGGTAAGCCGGG‑CCCCGCGGTGGGGGGACATGTGGGGGCA‑TTTTCCACCTTTGAGGGAAAAC‑GCGAAGCGGCAAAAACCCCGGGGGCCGATGAAGTTGTTAACTCCCGCAAAGCCGATGTGAGGGGGGGTCATCTGAAGAGTTTTGCTTTCATTTTTAAAACAGGAGATGCGCCACAtca > 2:507441/1‑248 (MQ=255) cGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATaa < 2:452080/248‑1 (MQ=255) gTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGGTTAAGCTGGC‑CCACGCGATGGGGGCCCATGTGGTGGCA‑TTTTCCACTTCTTGGGCCAAAC‑CCGAAGCGGCAAAAGCCCTGGGGGCCGGTGGAGTTTTTAAATCACGCCATGCCGCTGAGATGGGGGCTCCTCTGAAGAGATTCGATTTTCTTTTGAATACAGTAACTGCGGCACCTAAtct > 2:251367/1‑250 (MQ=255) gCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGAt < 1:516979/250‑1 (MQ=255) cAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGAtt < 2:346512/250‑1 (MQ=255) aaaGTGGGCGTGGTCGGCATCGGCGGTCTGGGGCATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGGACCTGTGGTGGCA‑TTTTCCACCTCTGAGGCCAAAC‑GCGGAGCGGCGAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGGTGAGGTGGCGGGTCATCTGAAGAGTTTCGATTTCATTTTTAATACAGTAGCTGCGCCACATAAACTCGCCGATTTTACCACCCTTCTGaaac > 2:363644/1‑248 (MQ=255) tCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGCTGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGGTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCATTTTTAATACAATAGCTGCGCCACATAATCTCGACGATTTTTCCACCTTGCTGAAGCGTGGTGGCACCag > 2:507660/1‑249 (MQ=255) ggcggTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCtg < 1:227878/249‑1 (MQ=255) cggTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCtggtt > 2:209407/1‑250 (MQ=255) tGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGAGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTCACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGCATTTACCAACTTGCTGAACCGAGAGTGCACCATGACGCTGGTTGGTgc > 2:714418/1‑250 (MQ=255) ggACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTgcg < 2:156487/249‑1 (MQ=255) ggACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTgc < 2:704149/248‑1 (MQ=255) | AACTACTGTGATCACATGACCGGCACCTATAACTCGCCGACGCCGGACGAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGC‑CCACGCGATGGGGGCACATGTGGTGGCA‑TTTACCACTTCTGAGGCAAAAC‑GCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTCATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGTGATGGCACCATGACGCTGGTTGGTGCG > NZ_CP009273/338907‑339401 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |