Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,659,062	0	C	A	34.5%	102.6 / 14.4	55	A37S (GCG→TCG)	gadX	acid resistance transcriptional activator GadX
Reads supporting (aligned to +/- strand):  ref base C (28/8);  new base A (0/19);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 7.91e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGTTCTACGGG  >  NZ_CP009273/3658814‑3659301                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                aaaGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGc                                                                                                                                                                                                                                                 >  2:131795/1‑249 (MQ=255) aaaGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGc                                                                                                                                                                                                                                                 >  1:22676/1‑249 (MQ=255)    gATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCaaa                                                                                                                                                                                                                                              >  2:584032/1‑249 (MQ=255)      ttAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAAc                                                                                                                                                                                                                                           <  2:630119/250‑1 (MQ=255)        aaTGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAAc                                                                                                                                                                                                                                           >  2:467582/1‑248 (MQ=255)               ttttACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAGGTCa                                                                                                                                                                                                                                    <  2:133120/248‑1 (MQ=255)                 ttACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAGGTCACCg                                                                                                                                                                                                                                 <  2:19297/249‑1 (MQ=255)                     gTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAGGTCACCGCCAt                                                                                                                                                                                                                             >  1:246433/1‑249 (MQ=255)                           tttCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAGGTCACCGCCATTaaaa                                                                                                                                                                                                                        >  2:8829/1‑248 (MQ=255)                              cTGCATAGCGAATATTCTTATTCTGCGATAGTTGCGCAACTTCCTAAAAAACAGAAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAATATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTAAACACACTTATCTCCTCGATTTTGGCTTG‑CATCCGCAAAAACCAGGTCACCGCCATTAAAATAGc                                                                                                                                                                                                                    <  1:698894/249‑1 (MQ=255)                                 cATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGata                                                                                                                                                                                                                >  2:165821/1‑250 (MQ=255)                                  aTAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAg                                                                                                                                                                                                                                        >  1:134874/1‑225 (MQ=255)                                   tAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAgg                                                                                                                                                                                                                                       <  2:134874/225‑1 (MQ=255)                                         aGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCAt                                                                                                                                                                                                        >  2:589363/1‑250 (MQ=255)                                          ttttCTTATTGTGCAATTGTTGCACACCTTCCTAAAAAACAGGAATATCTCTACGACAGGAATAAACGAGAAATGAAGAATCTTTTTTATGTACATGAAGTTTTAACGCCTCCCCCTTGAGCAGCGGAAAAAATAAAGAAAGCGTGTCCCTTGACACGAATAAAAAATTTACAACCCCCTTATCACCTCAAATTTGGCTTA‑CACCCGAAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCAtt                                                                                                                                                                                                       <  2:113711/248‑1 (MQ=255)                                          gATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCAt                                                                                                                                                                                                        >  2:342372/1‑249 (MQ=255)                                               ttATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGGGATGAACGAGTAATAAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAAAAATAATGAAAGCGTGTCCCTTGACACAAATAAAAAATTTACAACACACTTATCTCCTAGATTTTGGCTTC‑CACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                                                                                                                   <  1:497255/249‑1 (MQ=255)                                                            gtggcacAACTTCCTAAAAAACAGAAATTTCTCTACTACAGTGATGAACGAGTAATGAAAAAACTTTTTTATGTGCAAGAATATTTAATCCCCCCTCCTTGAGCATGGGGAAAAATAATAAAAGCGTGCCCCTTGAAACAAAAAAAAAATTTTAAACCCACTTTTCTCCTCAATTTTGGTTTG‑CACCCGAAAAACCCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatat                                                                                                                                                                                      <  1:319983/243‑1 (MQ=255)                                                            gTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGATTAATGAAGAATTTTTTTTATGTGCATGAAGATTTAATCCCTCCTCCTTGAGCATCGGGAAAAATAATGAAAGCGTGTCCCTTGACACAAATAAAAAATTTTCAACACACTTATCTACTCGATTTTGGCTTG‑CATCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatata                                                                                                                                                                                     <  1:8829/250‑1 (MQ=255)                                                                        ccTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTg                                                                                                                                                                          >  1:244976/1‑249 (MQ=255)                                                                          agAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAAAATCTTTTTTATGTGCATAAAGATTTAATGCCTCCTCCTTGAGCATGGGGAGAAATAATAAAAGCGTCTCCTTTGACACAAATAAAAAATTTTCAACACATTTATCTCCTCAAATTTGGCTTG‑CACCCAAAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCAt                                                                                                                                                                       <  2:669479/249‑1 (MQ=255)                                                                             aaaaCAGAAATATCCACACAAAGGAGAAGAATGAGTAAAGAAGAAACTTTTTTATGTGAATGAAGATTTAACGCCCCCCCCGTGACCATCGGAAAAAAAAAATAAAGCGTGCCCGTTGACAAAAATAAAAAATTTTAAACCCATTTTCTTACTC‑AATTTGGCTTGCCCCCCAAAAAAACCGGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACg                                                                                                                                                                    <  2:541436/250‑1 (MQ=255)                                                                               aaCAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGc                                                                                                                                                                   >  2:479922/1‑249 (MQ=255)                                                                                aCAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCaa                                                                                                                                                                 >  2:497776/1‑250 (MQ=255)                                                                                     aaTATCTCTCCTCAAGTGATGAAAGAGTAATGAAGAATTTTTTTTATGGGAATGAAGATTTAATGCCTCCTCTTTGGGAATGGGGAAAAATAATGAAAGCGTCTCCCTTGACACAAAAAAAAAATTTTCAACCCATTTCTCTACTCAATTTTGGCTTA‑CACCCAAAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGa                                                                                                                                                            <  1:273269/250‑1 (MQ=255)                                                                                               ctacAGTGATGAAGGAGTAATGAAGAATTTTTTTTATGTGCATGAAAATTTAATGCCCCCTCCTTGAGCATCGGGAAAAATAAGGAAAGCGTGTCCTTTGACACAAATAAAAAATTTTCAACACCCTTATCTACTCAATTTTGGCTTG‑CATCCGCAAAAACCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCAt                                                                                                                                                  <  1:740006/250‑1 (MQ=255)                                                                                               ctacAGTGATAAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAATCCCTCCTCCTTGAGCATGGGAAAAAATAAGGAAAGCGTTTCCCTTGACACAAATAAAAATTTTTCAACACCCTTTTCTACTCAATTTTGGCTTG‑CATCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTcc                                                                                                                                                     <  1:70935/247‑1 (MQ=255)                                                                                               ctacAGTGATAAACGAGAAATAAAAAATCTTTTTTATGTGCATAAAATTTTAATGCCCCCTCCTTGGGCATCGGGAAAAATAATAAAAGCTTTTCCCTTAACACAAATAAAAAATTTTCAACACTCTTATCTCCTCGAATTTGGCTTG‑CATCCGAAAAACCCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCAt                                                                                                                                                  <  1:275677/250‑1 (MQ=255)                                                                                                       atgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGAt                                                                                                                                          >  1:412472/1‑250 (MQ=255)                                                                                                        tgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGAtt                                                                                                                                         >  1:190671/1‑250 (MQ=255)                                                                                                             gAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCa                                                                                                                                      >  2:123413/1‑248 (MQ=255)                                                                                                                     aagaaACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACt                                                                                                                             >  1:405754/1‑249 (MQ=255)                                                                                                                               tttATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAAAAATAAAAAAAGCGTGTCCCTTAACACAAATAAAAAATTTTCAACACACTTATCTACTCAATTTTGGCTTG‑CATCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACa                                                                                                                   <  1:611400/249‑1 (MQ=255)                                                                                                                               tttATGTACATGAAGATTTAATGCCTCCTCCTTGAGCATCGGAAGAAATAAGGAAAGCGTTTCCCTTAACACAAATAAAAAATTTTCACCCCACTTATCTACTCGAATTTGGCTTG‑CACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACa                                                                                                                   <  1:605422/249‑1 (MQ=255)                                                                                                                                   tgggCATGAAGATTTAATGCCCCCCCCTTGAGCATCGGAAAAAATAAAGAAAGCGGTCCCCTTGACAAAAATAAAAAATTTTCAACACACTTATCTACTCGATTTTGGCTTG‑CATCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAAc                                                                                                              <  1:190576/247‑1 (MQ=255)                                                                                                                                            aattttAATGCCCCCCCCTGGAGAATGGGAAAAAAAAAGAAAAGCGTGCCCCTTAAAAAAAATAAAAAATTTTAAACACATTTATCTACTCAAATTGGGCTTG‑CACCCAAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGTCAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTg                                                                                                     <  1:620748/247‑1 (MQ=255)                                                                                                                                             gATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGt                                                                                                    >  1:663183/1‑250 (MQ=255)                                                                                                                                             gATTAAATACCTCCTCCTTTAGCAACGGGAGAAAAAATAAAGTCGTTTCCCTTAACACAAATAAAAATTTTACAACCCATTTATCACCTCAATTTTGGCTTG‑CACCCACAAAACCCAGGTCGCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGt                                                                                                    <  1:539526/250‑1 (MQ=255)                                                                                                                                              ttttAATCCCTCCTCCTTGAGCATCGGAAAAAAAAATAAAGGCTTTCCCCTTGAAAAAAATAAAAAATTTCCAACACATTTATCTCCTCAATTTTGGCTTG‑CATCAGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGtt                                                                                                   <  1:601919/249‑1 (MQ=255)                                                                                                                                                ttAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTtta                                                                                                 >  2:686819/1‑250 (MQ=255)                                                                                                                                                   atcccccctcctTGAGCCTCGGGAAAAATAATGAACGCGTCTCCCTTGACACAAATACAAATTTTTCACCACACTTATCTACCCAATTTTGGCTTG‑CACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTttatta                                                                                              <  1:175785/244‑1 (MQ=255)                                                                                                                                                      cccccccctGGAGCATGGGGAAAAAAAATAAAAGCGTTTCCCTTGACACAAATAAAAAATTTCCAACCCATTTATCTACTCGATTTTGGCTTG‑CACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAAt                                                                                            <  1:475484/243‑1 (MQ=255)                                                                                                                                                          ctcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAg                                                                                        >  1:683271/1‑249 (MQ=255)                                                                                                                                                            cctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCAc                                                                                     >  2:608609/1‑250 (MQ=255)                                                                                                                                                            cctTGAGAATCGGAAAAAATAATGAAAGCGTGTCCCTTGACACAAATAAAAAATTTTCAACACACTTATCTCCTCGATTTTGGCTTG‑CACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCAc                                                                                     <  1:683591/250‑1 (MQ=255)                                                                                                                                                                          agaAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAg                                                                       >  1:698450/1‑250 (MQ=255)                                                                                                                                                                              ataatGAAAGCGTGTCCCTTGACACGAATACAAACTTTTCAACACACTTATCTTCTCGAATTTGGCTTG‑CATCCGCAATAACCAGGTCACCGCCATTAAAATAGCGATATTCACCTTTAACCATGGTGTGTATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTATTGTAGCACGCCGCCCTCTTAAGTCa                                                                    >  1:247023/1‑249 (MQ=255)                                                                                                                                                                               aaatGAAAGCGTGTCCCTTGACACAAATAAAAAATTTTCAACACACTGATCACCTCGAATTTGGATGC‑CATCCACAAAAACCAGGTAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCaaa                                                                  <  1:132256/249‑1 (MQ=255)                                                                                                                                                                                            tCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGataaa                                                      >  1:122078/1‑249 (MQ=255)                                                                                                                                                                                               catcacaaaaaTACAAAATTTACAACACACTTATCTACTCGAATTTGTCTCA‑CATCCAAAAAAACAAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGAtaaataaa                                                  <  2:704546/241‑1 (MQ=255)                                                                                                                                                                                                       gAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAAc                                           >  1:154007/1‑249 (MQ=255)                                                                                                                                                                                                       aaaTAAAAAATTTAAAACACACTTATCTACTCAAATTTGGCTTC‑CATCCGAAAAAACCAGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACg                                          <  1:131795/249‑1 (MQ=255)                                                                                                                                                                                                             aaaaTTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCgg                                    >  2:345952/1‑250 (MQ=255)                                                                                                                                                                                                                 ttttCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAGGTCACCGCTATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGgag                                 >  1:510709/1‑249 (MQ=255)                                                                                                                                                                                                                              tATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAAt                   >  1:605561/1‑250 (MQ=255)                                                                                                                                                                                                                                        tctttGGCTTG‑CATCCTCAAAAACCTTTTCTCCGCCATTAAATTTGCGATATTCACCATTAACCATGGTGTGATTATATTTATGTCTTGCATACGCTATTTGACAATTCCCATGTATTGTTTTCATAGTTGACTTAATATTACTTAAACATTTTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTTATTGATAAATAAATTTTAACGAAGCGGGAGAACTGTATGATAATGTGTGATGtt         >  2:273744/3‑250 (MQ=255)                                                                                                                                                                                                                                                ttG‑CATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGTTCTACggg  >  1:723526/1‑249 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                AAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTG‑CATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGTTCTACGGG  >  NZ_CP009273/3658814‑3659301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑