Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,408,0180CT32.7% 108.0 / 17.9 55G56E (GGA→GAA) ralRtype I toxin‑antitoxin system endodeoxyribonuclease toxin RalR
Reads supporting (aligned to +/- strand):  ref base C (5/32);  new base T (18/0);  total (23/32)
Fisher's exact test for biased strand distribution p-value = 2.34e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAA‑TATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACAT‑GGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTA  >  NZ_CP009273/1407770‑1408263
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       
ataatCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTc                                                                                                                                                                                                                                                         <  2:449100/249‑1 (MQ=255)
 taatCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCa                                                                                                                                                                                                                                                       <  2:506473/250‑1 (MQ=255)
     cAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATTTATTAACAC‑CCCCCTTTTTTTTTTTAGTgg                                                                                                                                                                                                                                                   >  2:305364/1‑250 (MQ=255)
            gAAGTTTTAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAAATTTTTTTATTAAAAA‑CACCCTTATTATTTTCCTTGGttttttt                                                                                                                                                                                                                                            >  1:220283/1‑245 (MQ=255)
             aaatgttAATTTGAATACAATGCAATTCTCGAGTACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGttt                                                                                                                                                                                                                                            <  2:443901/244‑1 (MQ=255)
                          aaTACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCg                                                                                                                                                                                                                               <  2:541322/249‑1 (MQ=255)
                              cAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGc                                                                                                                                                                                                                          <  2:402031/250‑1 (MQ=255)
                                aTGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTTATTTTTTTACTTAACACACTCC‑TTCTTTTTTTCAGTGGTTCGTTTACTTCATCtttttcgtgttt                                                                                                                                                                                                                        >  1:228835/1‑241 (MQ=255)
                                aTGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTTCATTAACAA‑CTCCCTTTTTATTTTCTCTGGTTTGTTTTTTTCATCTTTTCTGTGctt                                                                                                                                                                                                                        >  1:119882/1‑250 (MQ=255)
                                 tGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTTTAATAACAA‑CCCACTTATTTTTTTCAGAGGTTTGTTTTTTCCATCTTTTGTGTGcttt                                                                                                                                                                                                                       >  1:104825/1‑249 (MQ=255)
                                   cAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTTCATGAAAAC‑CTCCCTTTTTTTTTTCATTTGGTTGTTTATTTCCTCTTTTTGGTGcttctt                                                                                                                                                                                                                     >  1:626953/1‑250 (MQ=255)
                                           gAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAAATTTTTAAATTAAAAC‑CCCCCTTTTTTTTTTCCATGGGTTGTTTTTTTCATTTTTTTGGTGGttttttttttctt                                                                                                                                                                                                             >  1:128808/1‑245 (MQ=255)
                                                  gAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATAAAAGAAAAC‑CACCCCTATTATTTTCACTGGGTTGTTTATTTCATTTTTTTAGTGGTTCtttttttctttccccc                                                                                                                                                                                                       >  1:383746/1‑238 (MQ=255)
                                                   aaGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTccacca                                                                                                                                                                                                       <  2:379115/248‑1 (MQ=255)
                                                   aaGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCAt                                                                                                                                                                                                      <  2:698729/249‑1 (MQ=255)
                                                   aaGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCAt                                                                                                                                                                                                      <  1:656553/249‑1 (MQ=255)
                                                       ttCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTATAATAACAC‑CACCACTTTTATTTTCTTTCGTTTGTTTATTTTCTCTTTCTTGTGTCTCTTTTTCTCTTTTTCCATaaact                                                                                                                                                                                                 >  1:182342/1‑247 (MQ=255)
                                                        tctcGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCg                                                                                                                                                                                                 <  1:123781/249‑1 (MQ=255)
                                                         ctcGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCgg                                                                                                                                                                                                <  1:707342/249‑1 (MQ=255)
                                                            gCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTAAATGAACAC‑CCCCCTTTTTTTTTTTCATGGTTCGTTTTTTTCTTTTTTTTTGTGCTTTTTTTTTATTTTCACCCTAAACCGtttg                                                                                                                                                                                            >  1:656361/1‑248 (MQ=255)
                                                            gCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGtt                                                                                                                                                                                              <  2:449018/248‑1 (MQ=255)
                                                             cAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCgg                                                                                                                                                                                           <  1:449171/250‑1 (MQ=255)
                                                              aaTTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTTATTTTTTATATTAACAC‑CCCCCCTTTTTTTTTTATTGGGTTGTTTTTTTTATTTTTTTTGTGCTTTTTTTTTTCTTTTACCATAACCAGTTTggg                                                                                                                                                                                          >  2:250400/1‑250 (MQ=255)
                                                              aaTTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCggg                                                                                                                                                                                          <  1:20920/250‑1 (MQ=255)
                                                                                  aCTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACtt                                                                                                                                                                       <  1:364778/249‑1 (MQ=255)
                                                                                       cTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTc                                                                                                                                                                  <  1:437792/249‑1 (MQ=255)
                                                                                       cTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTc                                                                                                                                                                  <  1:289141/249‑1 (MQ=255)
                                                                                           tctctGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTTCATTAACAC‑CTCCCTTTTTATTTTCTGTTGTTTTTTTTTTTCATCTTTTTTGTGCTTTTTTTTTTTTTCTTCCATTAACCGTTCTGGGTTTTCATTCTTTTCACTTTTCTCGGtga                                                                                                                                                             >  2:251761/1‑248 (MQ=255)
                                                                                                 gAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAa‑tat                                                                                                                                                         <  2:22456/247‑1 (MQ=255)
                                                                                                     cGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAA‑TATCCTGaaa                                                                                                                                                  <  1:64489/250‑1 (MQ=255)
                                                                                                          gCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTTCATGAACAC‑CCCCATTATTTTTTTCAGTGGTTTGGTTTTTTCCTTTTTTGTGTGCTTTTTTTTTTCTTCCCCCCTAAACGGCTTCGGATTTGGATCCGTTATAATTTTCTCGGTCA‑TATTCTGAAATGGCt                                                                                                                                             >  1:314024/1‑250 (MQ=255)
                                                                                                                 ttttACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTACATTAAACC‑CCCCCCTTTTTTTTTCTGTGGTTCGTTTTTTTTTTTTTTCTTGTGCTTCTTTTTTTCTTCCTCCATAACCCGTTTCGGGTTCTCCTCCCTTTCACCTCCCTCGGTAATTATCCT‑CAATGGCttttcccc                                                                                                                                      >  2:632026/1‑245 (MQ=255)
                                                                                                                                  ttttCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAA‑TATCCTGAAATGGCTTTCACCGTTACTGATGGACAAcc                                                                                                                      <  2:490974/249‑1 (MQ=255)
                                                                                                                                      cGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGCTCGGGATTCGCATCCGTTACACTTCGCTCGGTAA‑TATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACaa                                                                                                                  <  1:695060/249‑1 (MQ=255)
                                                                                                                                       gCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAA‑TATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACaaaa                                                                                                                <  1:278029/250‑1 (MQ=255)
                                                                                                                                            cTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTTAATTAAAAA‑CTCCATTATTATTTTCCGTGGTTTGTTTTTTTCATCTTTTGAGGGGTTTTTTTTTACTTCCACCATAACCCGTTCGGGGTTTACATCCGTTACAATTCGCTCGGTAA‑TATACTGAAAAGGCGTTCACCGCTACTGATGGGCCACCACAAAAAGGa                                                                                                            >  1:541352/1‑249 (MQ=255)
                                                                                                                                                tttCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTTAATTAACAC‑CCCCCTTATTTTTTTCAGTGGGTTGTTTTTTTCATCTTTTTAGTGCTTCTTTTTTTCTTTCACCCTAACCGGCTCGGGGTTTGCATCCGTTACACTTCTCTCGGGAA‑TATTCTTAAATGGCTTTTCCCCTTTCTGTTGGGAAAACACaaaaaagaaaagg                                                                                                       >  1:172961/1‑241 (MQ=255)
                                                                                                                                                  tCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAA‑TATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACAT‑GGt                                                                                                     <  1:609235/249‑1 (MQ=255)
                                                                                                                                                             gATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTTTAAGAACAA‑CTCCCCTATTTTTTTTAGTGGTTTGGTTTTTTCTTTTTTTGAGTGCTTCTTTTTTTTTTTTCCCATAACCGGTTCTGGGTTTTCATCCCTTTCACTTCTCTCCGTAA‑TATACTGATATGGGTTTTTCCGTTTCTTATTGGCAACCCCAAAAAAGACATGGGTTTAACtttttt                                                                                          >  2:303057/1‑247 (MQ=255)
                                                                                                                                                             gATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAA‑TATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACAT‑GGTTTAACATTGTc                                                                                          <  2:15003/249‑1 (MQ=255)
                                                                                                                                                                     atatCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAA‑TATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACAT‑GGTTTAACATTGTCATATCTCAt                                                                                 <  1:305364/250‑1 (MQ=255)
                                                                                                                                                                             tCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTATATTAAAAC‑CCCCCTTTTTTTTTTCTCTGGGTTGTTTTTTTTTTCTTTTTTGTGGTTTTTTTTTTTTTTCCCCCTAAACCGTTTGGGGTTTGTCTCCGTTTCCCTTCTTCCGGTCA‑TATTCTTAAATGGCTTTTACCGTTTATTCTTGACAACCACAAAAAAGACAT‑GGGTTTAAATTTTTATATTTTCTTAtttttt                                                                         >  2:570489/1‑249 (MQ=255)
                                                                                                                                                                                      tgggAGTGTCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAA‑TATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACAT‑GGTTTAACATTGTCATATCTCATAATTTTTCTCATaaaaa                                                                <  2:382694/248‑1 (MQ=255)
                                                                                                                                                                                        ggAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAA‑TATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACAT‑GGTTTAACATTGTCATATCTCATAATTTTTCTCATaaaaaa                                                               <  2:128808/249‑1 (MQ=255)
                                                                                                                                                                                            tGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAA‑TATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACAT‑GGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATAttt                                                          <  1:30938/250‑1 (MQ=255)
                                                                                                                                                                                            tGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAA‑TATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACAT‑GGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATAttt                                                          <  2:140265/250‑1 (MQ=255)
                                                                                                                                                                                                      cttTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAA‑TATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACAT‑GGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCg                                                <  1:177326/250‑1 (MQ=255)
                                                                                                                                                                                                                          ttttATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAA‑TATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACAT‑GGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCt                            <  2:626953/250‑1 (MQ=255)
                                                                                                                                                                                                                                 cATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAA‑TATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACAT‑GGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAattat                     <  1:610084/250‑1 (MQ=255)
                                                                                                                                                                                                                                   tGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAA‑TATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACAT‑GGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTc                   <  1:189278/250‑1 (MQ=255)
                                                                                                                                                                                                                                             ccATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAA‑TATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACAT‑GGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAAt           >  1:311525/1‑249 (MQ=255)
                                                                                                                                                                                                                                                 tattattTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAA‑TATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACAT‑GGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATc       >  2:201443/1‑249 (MQ=255)
                                                                                                                                                                                                                                                  attattTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAA‑TATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACAT‑GGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGa     >  2:619944/1‑250 (MQ=255)
                                                                                                                                                                                                                                                   ttattTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAA‑TATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACAT‑GGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGAt    <  2:293844/250‑1 (MQ=255)
                                                                                                                                                                                                                                                     attTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAA‑TATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACAT‑GGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTa  >  1:399725/1‑250 (MQ=255)
                                                                                                                                                                                                                                                      tttCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAA‑TATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACAT‑GGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTa  >  1:519235/1‑249 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       
ATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACAC‑CTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAA‑TATCCTGAAATGGCTTTCACCGTTACTGATGGACAACCACAAAATGGACAT‑GGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTA  >  NZ_CP009273/1407770‑1408263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑