| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,995,392 | 0 | G | A | 33.3% | 87.1 / 18.1 | 51 | intergenic (‑122/+199) | fliA/fliC | RNA polymerase sigma factor FliA/H48 family flagellin FliC |
| Reads supporting (aligned to +/- strand): ref base G (31/3); new base A (0/17); total (31/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.72e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTC‑ACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTAGCT > NZ_CP009273/1995143‑1995636 | ttgTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTC‑ACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAg < 1:402165/248‑1 (MQ=255) aaGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTC‑ACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGaaaa < 1:403688/249‑1 (MQ=255) aGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGGACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTC‑ACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACCGGTAAATTCCAGGCAGaaaaa > 2:243735/1‑249 (MQ=255) gCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTC‑ACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CC‑ccgcc > 2:290004/1‑250 (MQ=255) cTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTC‑ACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CC‑ccgccg > 2:440089/1‑250 (MQ=255) gcgGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTC‑ACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CC‑CCGCCGGTg > 1:345740/1‑250 (MQ=255) cgGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTC‑ACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CC‑CCGCAGGTgg > 2:306540/1‑250 (MQ=255) aCCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTC‑ACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCAc > 1:44229/1‑250 (MQ=255) cAGGCGAAAGCCTGCGTGACGCACCAGCGGAACATAACGCTGCCACAGCGATTTTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTC‑ACGATAAACAGCCCTGCGTTATATGAGTTAGCGGCATGATTATCCTTTTCTGCAGGTTTTTA‑AATCGGACGATTAGTGGGTAAAATGAGGGTTTATTTGGGGG‑TTACAGGTAAATCCCAGGAAAAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTg < 1:4104/249‑1 (MQ=255) gACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTC‑ACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTa > 2:70890/1‑250 (MQ=255) gACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTC‑ACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTa > 2:599208/1‑250 (MQ=255) cAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTA‑ACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGATTTT‑AATCGGACGATTAGTGGGTGAAATGTGGGTTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAACA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGATGcc > 2:595063/1‑250 (MQ=255) aaCGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTC‑ACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGCTgcggcg > 2:587830/1‑249 (MQ=255) cTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTC‑ACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAAc > 1:117606/1‑250 (MQ=255) agagcggttttttATCCATTACACCTTCAGGGGTATAGAGTGATTTC‑CAGATAAACACCCTGGCGTTATATGAGTTATCGGAAGGTTTATCCGTTTCGGCGGGGTTTTA‑AAGCGGACGATGAGGGGGAAAAAGGGGGGGTTTTTTGGGGG‑TTAAAGGAAAATTCCAGGAAAAAAAAAC‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCtt < 2:200087/240‑1 (MQ=255) acaGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTC‑ACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGAGTTTTT‑AATCGGACGATTAGGGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CC‑ACGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCtt < 2:568368/250‑1 (MQ=255) aGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTC‑ACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTAt > 1:332369/1‑250 (MQ=255) gAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTC‑ACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCg > 2:173242/1‑250 (MQ=255) tACACCTTCAGCGGTATAGAGTGAATTC‑ACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgt > 1:395734/1‑250 (MQ=255) cacCTTCAGCGGTATAGAGTGAATTC‑ACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgtg > 1:739044/1‑249 (MQ=255) cggCGGTTAAGAGTAAATGC‑AAGAAAAACAGCCTTGCTTTATAGGAGTGATCGGCATGATTATCGGTTCTGGCAGGTTTTTT‑AACCGGACGATTGGGGGGTAAAAGGGGGGTTTTTTTGGGGG‑TAACAGGAAAATCCCAGGAAAAAAAAAC‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAAt < 2:61939/246‑1 (MQ=255) cGGTATAGAGTGAATTC‑ACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGGCGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTcca > 2:240310/1‑247 (MQ=255) ggtaaTTCAACGATAAACACCCGTGTGTTATATGAGTAACGGGTTTAATTAT‑CGTTTTTGGGGGGTTTTTAAAGCGGAATATGGGGGGGAGAAAGAAGGGTTTTTTTGGGGT‑TTACAGGTAAATTCAAGGCAAAAAAAAC‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCa < 2:633025/247‑1 (MQ=19) gTGATTTA‑ACTAAAAACAGCCCTGTTTTATATGGTTTATCGGCATGTTTATCGGTTGCGGTGGGTTTTTT‑AAGGGGAGGATTAGGGGGTAAAAGGGGGGTTTTTTTGGGGG‑TTAC‑GGAAAAATCCAAGAAAAAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCaa < 2:503667/250‑1 (MQ=19) taaaTTC‑ACGATAAACAGCCCGGCTTTATATGAGTTAGCGGCATGATTATCCGTTTCTGCAGGTTTTTA‑AAGGGGACTATTAGGGGGTAAAAGGAGGGTTTTTTGGGGGG‑TTAAAGGAAAATTCCAGGCAAAAAAAAC‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAt < 1:17318/248‑1 (MQ=37) cc‑ACGATAAACGGCCCTGCGTTATAGGATTTTTCGGCATGATTTCCCGTTTCTGCGGGTTTTTT‑AATGGGAGTATTGGGGGGTGAAATG‑GGGGTTTTTTGGGGGTTTACAGGAAAATCCCAGGAAAAAAAAAC‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCaa < 1:411274/248‑1 (MQ=37) ggCCCTGCGTTATAGGATTTATCGGATTTATTATCTTTTTCTGCGGGTTTTTT‑AACCGGAGGTTTAGGGGGTAAAATGGGGGTTTTTTTGGGGG‑TTACAGGTAAATCCCAGGCAAAAAAAAC‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTg < 2:65343/249‑1 (MQ=37) tatGATTTATCGGCATGTTTCTCCGTTTCGGCGGGGTTTTT‑AATCGGACGATTAGGGGGTAAAATGAGGGTTTATTTGGGGT‑TTACAGGAAAATCCCAGGAAAAAAAAAC‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgcagc < 1:570518/249‑1 (MQ=37) tGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAAATTGTAGGCCTGATAAgcgcagcgca > 1:357124/1‑250 (MQ=255) ttATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTa > 1:441888/1‑149 (MQ=255) ttgtggCATTATTTTCAGTTTGTGCGGGGTTTTA‑AAGGGGACGATGAGGGGGTAAAATGAGGGTTTTTTTGGGGG‑TTACAGAAAAATTCCAGACAAAAAAAAC‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAg < 2:480934/245‑1 (MQ=25) tctgtaTGAGTTTCCGTTTGTGCAGGTGTTTG‑AATGGGTCTATTGGGGGGTAAAAGGGGTGTTTTTTTGGGGG‑TTACAGTTAAATCCCGGGAAAAAAAAAC‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGc < 2:561495/244‑1 (MQ=21) ggCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAAt > 1:225288/1‑250 (MQ=255) aGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAgccc > 2:258806/1‑245 (MQ=255) cGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGt > 2:186011/1‑249 (MQ=255) gtagtggtgagagAAAGGGGGGGTAATTTGGGGG‑TAGCA‑GTACATGGCACGGAAAAAAAAA‑CCGCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAGTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATGTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACaa < 2:207301/238‑1 (MQ=25) gtaTTAGGGGTGGAAAGGGGGGGTTATTGGGGGT‑TTACAGGTAAATTCCAGGCAAAAAAAAC‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACaa < 1:626914/248‑1 (MQ=38) tGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTaa > 2:218275/1‑250 (MQ=255) aaaGGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAAAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTgc < 1:63506/250‑1 (MQ=255) aaaGGAGGGGTTATTGGGGGG‑TTACAGGAAAATCCCAGGCAGAAAAAAC‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTg < 1:440089/249‑1 (MQ=255) aTGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTgcag > 1:131768/1‑250 (MQ=255) gggtTTTTTGGGGGG‑TAACAGGTAAATTCCAGGCAAAAAAACC‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCaga < 2:353202/250‑1 (MQ=255) gggTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTgcagca > 1:522862/1‑247 (MQ=255) ggTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAgaga > 2:729920/1‑250 (MQ=255) tgggggg‑TTA‑AGGGAAAATGCAGGGAAAAAAAAACCC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGaa < 2:619011/247‑1 (MQ=38) tGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAAcc > 1:713273/1‑250 (MQ=255) ggTAAATTCCAGGCAGAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTAc > 2:150221/1‑250 (MQ=255) tAAATTCCAGGCAGAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCt > 2:500078/1‑250 (MQ=255) aaTTCCAGGCAGAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTgg > 2:179825/1‑250 (MQ=255) tCCAGGCAGAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGt > 1:310040/1‑248 (MQ=255) cagagCAAAAAGACA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTa < 2:569600/245‑1 (MQ=255) aGGCAGAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTAGCt > 1:601953/1‑250 (MQ=255) | TCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTC‑ACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAA‑CC‑CCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACCCTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTAGCT > NZ_CP009273/1995143‑1995636 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |