Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,221,7540TC50.0% 31.4 / 15.3 42pseudogene (484/1436 nt)mdtQmultidrug resistance outer membrane protein MdtQ
Reads supporting (aligned to +/- strand):  ref base T (13/8);  new base C (6/15);  total (19/23)
Fisher's exact test for biased strand distribution p-value = 6.16e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAA‑GACCGTGTTTAA‑CGCCGCCTGGGTTTGCCA‑CTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTT‑GCTCGCGTTCCGCCGCCCGT‑GCTTTAACCGTACCCAGG‑CGGGCA‑GTAACCTCCGCCCGATTCTTTCCCCAGATATCGAG‑ATGCCAGCCCGCCG‑TTAAGCCA‑AAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCC  >  NZ_CP009273/2221515‑2222002
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                           
tGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAA‑GACCGTGTTTAA‑CGCCGCCTGGGTTTGCCA‑CTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTT‑GCTCGCGTTCCGCCGCCCGT‑GCTTTAACCGTACCCAGG‑CGGGCA‑GTAACCTCCGCCCGAt                                                                                                                                                                                                                                                    <  1:625011/249‑1 (MQ=255)
              tCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAA‑GACCGTGTTTAA‑CGCCGCCTGGGTTTGCCA‑CTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTT‑GCTCGCGTTCCGCCGCCCGT‑GCTTTAACCGTACCCAGG‑CGGGCA‑GTAACCTCCGCCCGATTCTTTCCCCAGatat                                                                                                                                                                                                                                     <  1:649913/250‑1 (MQ=255)
                 gTTTCCACGCCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAA‑GACCGTGTTTAA‑CGCCGCCTGGGTTTGCCA‑CTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTT‑GCTCGCGTTCCGCCGCCCGT‑GCTTTAACCGTACCCAGG‑CGGGCA‑GTAACCTCCGCCCGATTCTTTCCCCAGATATCga                                                                                                                                                                                                                                  <  1:704603/250‑1 (MQ=255)
                     ccACACCTTCAACGGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGTTCACGGTAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAA‑GACCGTGTTTAC‑CGCCGCCTGGGTTTGCCA‑CTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTT‑GCTCGCGTGCCGCCGCCCGT‑GCTTTAACCGTACCCAGG‑CGGGCA‑GTAACCTCCGCCCGATTCTTTCCCCAGATATCGAG‑At                                                                                                                                                                                                                              <  2:540503/249‑1 (MQ=255)
                      cacaCCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAA‑GACCGTGTTTAA‑CGCCGCCTGGGTTTGCCA‑CTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTT‑GCTCGCGGTCCGCCGCCCGT‑GCTTTTACCGTACCCAGG‑CGGGCC‑GTAACCTCCGCCCGATTCTTTTCCCCGATATCggg‑aggt                                                                                                                                                                                                                            >  1:613779/1‑247 (MQ=255)
                        cacCTTCAACTGAAGAAGTAATCCCGTTCTGATATGGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAA‑GACCGTGTTTAA‑CGCCGCCTGGGTTTGCCA‑CTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTT‑GCTCGCGTTCCGCCGCCCGT‑GCTTTAACCGTACCCAGG‑CGGGCA‑GTAACCTCCGCCCGATTCTTTCCCCAGATATCGAG‑ATGCCa                                                                                                                                                                                                                          <  2:586548/250‑1 (MQ=255)
                         acCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAA‑GACCGTGTTTAA‑CGCCGCCTGGGTTTGCCA‑CTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTT‑GCTCGCGTTCCGCCGCCCGT‑GCTTTAACCGTACCCAGG‑CGGGCA‑GTAACCTCCGCCCGATTCTTTCCCCAGATATCGAG‑ATGCCAg                                                                                                                                                                                                                         <  2:385652/250‑1 (MQ=255)
                              cAACTGAAGAAGTAATCCCGTTCTGATATAGGTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAA‑GACCGTGTTTAA‑CGCCGCCTGGGTTTGCCA‑CTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTT‑GCTCGCGTTCCGCCGCCCGT‑GCTTTAACCGTACCCAGG‑CGGGCA‑GTAACCTCCGCCCGATTCTTTCCCCAGATATCGAG‑ATGCCAGccc                                                                                                                                                                                                                      <  2:734311/248‑1 (MQ=255)
                                      gaagTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAA‑GACCGTGTTTAA‑CGCCGCCTGGGTTTGCCA‑CTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTT‑GCTCCCGGTCCGCCGCCCCT‑GCTTTTACCGTACCCCCG‑CGGGGC‑GGAACCTCCCCCCCCCTTTTTCCCCCGATAACGAG‑ATGCCCGCCCCCCG‑CTTAGc                                                                                                                                                                                                           >  2:464861/1‑250 (MQ=255)
                                        agTAACCCCGTTCTGATATAGCTGGCGATCGGTCGCTATAAGGGTTTCTTGCTTTTTTTCTATTTGCTGCAA‑GCCCGTGTTTAC‑CGCCGCCTGGGTTTCCCA‑CCCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTT‑GCTCGCGTCCCGCCGCCCGG‑TCTTTACCCGTACCCGGG‑CGGGCA‑GTACCCCCCCCCCGATTCTTTCCCAAGATATCGAG‑ATGCCAGCCCGCCG‑TTAAGCCa                                                                                                                                                                                                         <  2:34341/250‑1 (MQ=255)
                                           aaTCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAA‑GACCGTGTTTAA‑CGCCGCCTGGGTTTGCCA‑CTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTT‑TCTCGCGTTCCCCCCCCCCC‑GCTTTTACCCTACCCAGG‑CGGGCG‑GTAACCCCCCCCCCATTCTTTTCCCCGACATACAG‑ATGGCACGCCCCCG‑CTACGCCc‑aa                                                                                                                                                                                                      >  2:365896/1‑249 (MQ=255)
                                           aaTCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAA‑GACCGTGTTTAA‑CGCCGCCTGGGTTTGCCA‑CTCCCAGTACA‑GGCGGGCTACGCCGCCAGCCCGCAATTGGCGGGGTT‑TCTTGGGTTCCGCCGCCCCG‑GCCTTTACCGTAACCACG‑CGGGGG‑GTAAACCCCCCCCCCTTCTTTCCCCCGACATCTAG‑ATGCGAGcccccccgtttgcccaa                                                                                                                                                                                                        >  2:298568/1‑238 (MQ=255)
                                            aTCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAA‑GACCGTGTTTAA‑CGCCGCCTGGGTTTGCCA‑CTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTT‑GGTCGCGTTTCGCCGCCCGT‑GCGTTAAACGTACCCAGG‑CGGGGA‑GGAAACTCCGCCCCATTCTTTTCCCCGATATAGAG‑ATGCCAGCCCGCCC‑GTAAGCCc‑aaa                                                                                                                                                                                                     >  1:598147/1‑249 (MQ=255)
                                                    cTGATATCGCTGGCGCGGTGTCGCGATAATGGGTTTCTGCTCTTTTTCTTTTTGCTGCAA‑GACTGTTTTTAC‑CGCCCCCTGGTTTTGCCA‑CCCCCAGTACA‑GGCGGGCTCCGCTGCCAGCCAGCATTTGGCGGGTTT‑GCTCGCGTCCCCCCCCCCGT‑GCTTTAACCGTCCCCAGG‑CGGGCA‑GTACCCCCCGCCCGATTCTTTCCCCATATATCGAG‑ATGCCAGCCCGCCG‑TTAAGCCA‑AAAGTACCGtt                                                                                                                                                                                             <  2:705326/249‑1 (MQ=255)
                                                      gATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAA‑GACCGTGTTTAA‑CGCCGCCTGGGTTTGCCA‑CTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTT‑TCTCGCGTTCCGCCGCCCCT‑GCTTTTAACCTACCCAGG‑CGGGCA‑GGAACCCCCGCCCGATTCTTTCCCCAGATATCGCG‑ATGCCAGCCCGCCG‑CTAAGCCA‑AAAGAACCCtttgg                                                                                                                                                                                          >  1:202227/1‑247 (MQ=255)
                                                                  attcggccggtttAAGGGTTTTCGTCTCTTTTTCAATTTGCGGCAG‑GCCCGTTTTTAC‑CCCCGCCTGGG‑TTGCCACCCCCCAGCACA‑GGCGGGTTCCGTTGCCACCCGCCATTTGGCGTGTTT‑CCCCGCGTTCCCCCGCCCGT‑TCTTTACCCGTCCCCGGG‑GGGGAA‑CTACCCCCCCCCCGATTCTTTCCCCAGATGTCGAG‑ATGCCAGCCCGCCG‑TTAAGCCA‑AAAGTACCGTTGGTGTACCACGGGc                                                                                                                                                                               <  2:273305/237‑1 (MQ=255)
                                                                                   gttttCTCCTCTTTTTCT‑TTTGCCGGAACGACCGTGTTGAG‑CCCCCCCGGGTTTTGCCC‑CCCCAAGTACA‑GGGGGCCTACGCTGCCAGCCAGAAATGGGCGGGTTT‑GCTCGCTTCCCCCCCCCCGT‑GTTTTACCCGTACCCAGG‑CGGGAA‑GTACCCCCCCCCCGATTCTTCCCCCAGATACCGAG‑ATGCCGGCCCGCCG‑TTAAGCCA‑AAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCg                                                                                                                                                             <  2:282709/247‑1 (MQ=255)
                                                                                                        ggctgcAA‑GCCCGTGTTTAC‑CGCCGCCTGGGTTTGCCC‑CCCCCAGTACA‑GGCGGGCTCCGCTGCCAGCCAGCAATTGGCGGGTTT‑GCTCGCGTTCCGCCGCCCGT‑GCTTTACCCGTCCCCGGG‑CGGGCA‑GTACCCCCCGCCCGATTCTTCCCCCAGATATCGAG‑ATGCCAGCCCGCCG‑TTAAGCCA‑AAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGcc                                                                                                                                                              <  1:32390/227‑1 (MQ=255)
                                                                                                              caggccggtgtTTAC‑CGCCGCCTGGTTTTCCCC‑CTCCCAG‑ACAGGGCGGGTTCCGCGCCCAGCCACCAATTGGCGGTTTT‑GCCCGCGTCCCCCCGCCGGT‑TCTTTAACCGCCCCCGGG‑GGGGCA‑GCACCCCCCGCCCGATTCTTCCCCCAGATATCGAG‑ATGCCGGCCCGCCG‑TTAAGCCA‑AAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCAtt                                                                                                                                   <  2:22952/243‑1 (MQ=255)
                                                                                                                aagccctgtTTAAGCGCCCCCTGGTTTCGCCC‑CCCCGAGTACG‑GGCGGGCTCCGCTCCCAGCCAGCATTGGGCGGGTTT‑GCTCGCGTCCCGCCCCCCGT‑GTTTTACCCGTCCCCGGG‑GGGGAG‑GTACCCCCCCCCCGTTTCTTCCCCCATATATCGAG‑ATGCCAGCCCGCCG‑TTAAGCCA‑AAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTa                                                                                                                                  <  2:633724/244‑1 (MQ=255)
                                                                                                                       gtTTAA‑CGCCGCCTGGGTTTGCCA‑CTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTT‑TCTCCCGGTCCGCCGCCCCG‑GCTTTTAACGTACCCCGG‑CGGGCG‑GTAACCTCCCCCCCGTTTTTTCCCCCGATATCGAGAATGCAACCCCCCCC‑TT‑AGCCACAAAGAACCTTTGG‑GTACCCCCGGCCCGTCCTACCCCCGGCCGGCTCGTTCCTGGCAAACGCCCCCCCTAAGCCctct                                                                                                                          >  2:365610/1‑250 (MQ=255)
                                                                                                                        tttAA‑CGCCGCCTGGGTTTGCCA‑CTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTT‑GCTCGCGTTCCGCCGCCCGT‑GCTTTAACCGTACCCAGG‑CGGGCA‑GTAAACTCCGCCCGATTCTTTTCCCAGATATCGAG‑ATGCCAGCCCGCCG‑TTAAGCCA‑AAAGTACCGTTGGGGTACCACCGGGCGGGCGGACCTGCGGCCGGGTCGTTCAGAGCAAACGGCCCCATTAAGCCCTCTg                                                                                                                         >  1:385379/1‑250 (MQ=255)
                                                                                                                        tttAA‑CGCCGCCTGGGTTTGCCA‑CTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTT‑GCCCGCGTTCCCCCCCCCCTGGCTTTAACC‑TACCCACG‑CGGGGAGGAAACCCCCCCCCGTTTCTTTCCCCGGTATTGGGG‑AGCCCGGCCCCCCG‑TTAGCCCA‑AAAAAACCGTTGGGGTACAACGGGCCGGCGGCACCCCCGCCCGGTTGGTTCAGGGCAAAGGCCCCCCCTAAGcctcct                                                                                                                          >  2:321139/1‑250 (MQ=255)
                                                                                                                        tttAA‑CGCCGCCGGGGTTTGCCC‑CCCCCAGTACG‑GGCGGGCTACGCTGCCAGCCAGAAATTGGCGGGTTT‑GCTCGCGTTCCGCCGCCCGT‑GCTTTACCCGTACCCAGG‑CGGGTA‑GTACCCCCCGCCCGATTCTTTCCCCAGATATCGAG‑ATGCCAGCCCGCCG‑TTAAGCCA‑AAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCt                                                                                                                          <  1:77084/249‑1 (MQ=255)
                                                                                                                                              cA‑CTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGCTT‑TTTCGCGGTCCGCCGCCCGT‑GCTTTTAACGCACCCCGG‑CGGGGA‑GTAACCCCCCCCCCCTTCTTTTCCCCGATATTGCG‑GAGCCCACCCGCCGCTGAACCAA‑AAAAAACCCTTGG‑GGACCACCGGGCGGGCGGAACCGCCGCCGGGGAGGTCCGAGGAAACAGCCCCCCTAAACCCTCTTCCGGCCCTTTTTTCCGccccc                                                                                                    >  2:474248/1‑249 (MQ=255)
                                                                                                                                                         tccgggCGGGCTCCGCGGCCACCCAGCAATTGGCGGGTTG‑CCCCGCGTCCCCCCCCCCGT‑GCTTAACCCGTCCCCAGG‑CGGGCA‑GTACCCCCCGCCCGTTTCTTCCCCCAGATATCGAG‑ATGCCAGCCCGCCG‑TTAAGCCA‑AAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATccgccg                                                                                           <  1:249803/246‑1 (MQ=255)
                                                                                                                                                               cGGGCTACGCTGCCAGCCAGCAATGGGCGGTTTT‑GCTCGCTTCCCGCCGCCCGT‑GCTTTAACCGTCCCCAGG‑GGGGAA‑GTACCCCCCGCCCGATTCTTTCCCCAGATATCGAG‑ATGCCAGCCCGCCG‑TTAAGCCA‑AAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGt                                                                                     <  1:474729/250‑1 (MQ=255)
                                                                                                                                                                gggCTACGCTGCCAGCCAGCAATTGGCGGGGTT‑GCTCGCGTTCCGCCGCCCGT‑GCTTTAACCGTACCCAGG‑CGGGCA‑GGAACCTCCGCCCGGTTCTTTCCCCCGATATCGAG‑ATGCCAGCCCGCCG‑TTTAGCCA‑AAAGTACCGGTTGTGTACCACGGGGCGGGCGTACCTGCGGCCCGGTCCGTCAGAGCAAACGGCCCCCTTAAGCCTTCCTCCGGCCATTTTTGGCGCTCCACAACCGACGGAAAggc                                                                                    >  2:552939/1‑248 (MQ=255)
                                                                                                                                                                                caggAATTGGGGGTTTG‑GCTCGCGTGCCGCCGCCCGT‑GCTTTACCCGCACCCAGG‑CGGGCA‑GAACCCCCCGCCGAATTTTTCCCCCAAATATGGAG‑ATGCCAGCCCGCCG‑TTAAGCCA‑AAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATcc                                                                    <  1:209230/250‑1 (MQ=255)
                                                                                                                                                                                        ggCGGGCTT‑GCTCGCGCTCCGCCGCCCGT‑GCTTTAACCGTACCCAGG‑CGGGCT‑GTAACCCCCGCCCCATTCTTTTCCCAGATTTCGAG‑ATGCCAACCCGCCG‑TTAAGCCA‑AAAACACCTTTTGCGTACCCCGGGCCGGTCGGACCTGCGGCCCGCACGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGGCAATTTTTGCCGCTCCATATCCGCCGAAAAATCGATCCCCGGAGCAACCAGAGTGGc                                                            >  2:493166/1‑250 (MQ=255)
                                                                                                                                                                                         gggggTTG‑GCGCGCGTCCCGCCGCCGGA‑CCTGTAACCG‑GCCCAGGCCGGGCA‑GACCCCCCCCCCCGCTTCTTCCCCCATATATGGAG‑ATGCTGTCCCGCCG‑TTAAGCCA‑AAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCa                                                           <  2:318227/248‑1 (MQ=255)
                                                                                                                                                                                         gCGGGTTT‑GCTCGCGTTCCGCCGCCCGT‑GCTTTAACCGTACCCAGG‑CGGGCA‑GTAACCTCCGCCCGATTCTTTCCCCAGATATCGAG‑ATGCCAGCCCGCCG‑TTAAGCCA‑AAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGc                                                            >  1:416350/1‑249 (MQ=255)
                                                                                                                                                                                          gtggTTTGGCTCGCTTTCCCCCCCCCGT‑GCTTAAACGGTCCCCGGG‑CGGGCA‑TTACCCCCCGCCCGATTCTTCCCCCAGTTAGCGAG‑ATGCCGGCCCGCCG‑TTAAGCCA‑AAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCa                                                           <  2:131468/248‑1 (MQ=255)
                                                                                                                                                                                           gggTTT‑GCTCGCTTTCCCCCCCCGGT‑GCTTTAACCGTCCCCGGG‑CGGGCA‑TTACCCCCCGCCCGATTTTTCCCCCAGATATCGAG‑ATGCCAGCCCGCCG‑TTAAGCCA‑AAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAAc                                                         <  1:336602/250‑1 (MQ=255)
                                                                                                                                                                                             gTTT‑GCTCGCGTTCCGCCGCCCGT‑GCTTTAACCGTACCCAGG‑CGGGCA‑GTAACCTCCGCCCGATTCTTTCCCCAGATATCGAG‑ATGCCAGCCCGCCG‑TTAAGCCA‑AAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTg                                                       >  1:414171/1‑250 (MQ=255)
                                                                                                                                                                                                   cTCGCGTTCCCCCCCCCGT‑TTTTTAACCGTCCCCAGG‑CGGGCT‑TTAACCCCCCCCCCATTCTTCCCCCAGATTGCGAG‑AGCCCAGCCCGCCG‑TTAAGCCA‑AAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTc                                                  <  1:290767/250‑1 (MQ=255)
                                                                                                                                                                                                               cgccCGT‑GCTTTAACCGTACCCAGG‑CGGGCA‑GTAACCTCCGCCCGATTCTTTCCCCAGATATCGAG‑ATGCCAGCCCGCCG‑TTAAGCCA‑AAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCtt                                        >  2:693306/1‑248 (MQ=255)
                                                                                                                                                                                                                gccCGT‑GCTTTAACCGTACCCAGG‑CGGGCA‑GTAACCTCCGCCCGATTCTTTCCCCAGATATCGAG‑ATGCCAGCCCGCCG‑TTCAGCCA‑AAAGTACCGGTGGTGTACCACGGGCCAGTCGTACCTGCGGCCGGAACGTTCCGAGCAAAAGGCCCCCATAAGCATGCTGCCGAAATTTTTTGCCGATCCATATCCGCCGAAAAGTCGTTCTCAGGACCGTCCTGAGTGGCAGCTGCCTTCGCCTTGGCTTccg                                     >  2:715891/1‑248 (MQ=255)
                                                                                                                                                                                                                   cGT‑GCTTTAACCGTACCCAGG‑CGGGCA‑GTAACCTCCGCCCGATTCTTTCCCCAGATATCGAG‑ATGCCAGCCCGCCG‑TTAAGCCA‑AAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCagc                                    >  1:160410/1‑248 (MQ=255)
                                                                                                                                                                                                                                       gg‑CGGGAA‑GAACCCTCCGCCAAATTCTTCCCCCAGATAGGGAG‑AGGCCAGCCCGCCG‑TTAAGCCA‑AAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGc               <  1:151751/250‑1 (MQ=255)
                                                                                                                                                                                                                                                     ccTCCGCCCGATTCTTTCCCCAGATATCGAG‑ATGCCAGCCCGCCG‑TTAAGCCA‑AAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATc   >  1:47924/1‑250 (MQ=255)
                                                                                                                                                                                                                                                      cTCCGCCCGATTCTTTCCCCAGATATCGAG‑ATGCCAGCCCGCCG‑TTAAGCCA‑AAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATcc  >  2:229747/1‑250 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                           
TGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAA‑GACCGTGTTTAA‑CGCCGCCTGGGTTTGCCA‑CTCCCAGTACA‑GGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTT‑GCTCGCGTTCCGCCGCCCGT‑GCTTTAACCGTACCCAGG‑CGGGCA‑GTAACCTCCGCCCGATTCTTTCCCCAGATATCGAG‑ATGCCAGCCCGCCG‑TTAAGCCA‑AAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATCC  >  NZ_CP009273/2221515‑2222002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑