Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,372,4470CT32.7% 71.1 / 19.8 56E98K (GAA→AAA) menD2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylic‑acid synthase
Reads supporting (aligned to +/- strand):  ref base C (6/31);  new base T (18/0);  total (24/32)
Fisher's exact test for biased strand distribution p-value = 9.34e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTCT‑CC‑GGTTAACCC‑GGCTTCAATCA‑GTGCCGGATAGAG‑ATTTG‑CCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCG‑AAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAA‑CGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTA  >  NZ_CP009273/2372204‑2372686
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       
ccATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTCT‑CC‑g                                                                                                                                                                                                                                                   <  2:731072/248‑1 (MQ=255)
  aTTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTCT‑CC‑GGTTa                                                                                                                                                                                                                                               <  1:471169/250‑1 (MQ=255)
          aTACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGGTTAG‑AG‑AGACAGGTTTTTT‑CC‑CGTTAACAC‑gggc                                                                                                                                                                                                                                      >  2:20265/1‑248 (MQ=255)
               gCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTACGCGG‑AATAGGTTTTTTT‑CC‑GGTTTCCCC‑gcgctcac                                                                                                                                                                                                                                  >  2:691478/1‑243 (MQ=255)
                cGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGGTAAG‑GG‑AATTCGGTTTTTT‑CC‑GGGTTACCC‑GGCTTCAAt                                                                                                                                                                                                                                 >  1:225276/1‑249 (MQ=255)
                 ggTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGCAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGGGGAG‑GG‑GATAAGGTTTTTT‑CCGGGTTAAcccagtgccaa                                                                                                                                                                                                                                   >  2:287776/1‑240 (MQ=255)
                  gTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTTAG‑AG‑AATCAGGTTTTCT‑CC‑GGTTTAACC‑GGCTTCAATCaa                                                                                                                                                                                                                              >  1:148508/1‑249 (MQ=255)
                          aaCGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTCT‑CC‑GGTTAACCC‑GGCTTCAATCA‑GTGCCGGAt                                                                                                                                                                                                                     <  1:202478/250‑1 (MQ=255)
                          aaCGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTCT‑CC‑GGTTAACCC‑GGCTTCAATCA‑GTGCCGGAt                                                                                                                                                                                                                     <  2:713274/250‑1 (MQ=255)
                              ggCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTCT‑CC‑GGTTAACCC‑GGCTTCAATCA‑GTGCCGGATagag                                                                                                                                                                                                                 <  2:741700/250‑1 (MQ=255)
                                     gATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTTAG‑AG‑AATCAGTTTTTTT‑TC‑GGGTAACCC‑GGCTTCAATCA‑GTGCCGGGTAGAG‑ATTTG‑cc                                                                                                                                                                                                        >  1:175101/1‑250 (MQ=255)
                                         tGGACTCCCGCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTCT‑CC‑GGTTAACCC‑GGCTTCAATCA‑GTGCCGGATAGAG‑ATTTG‑CCAccg                                                                                                                                                                                                    <  2:559002/250‑1 (MQ=255)
                                         tGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTCT‑CC‑GGTTAACCC‑GGCTTCAATCA‑GTGCCGGATAGAG‑ATTTG‑CCAcc                                                                                                                                                                                                     <  1:405895/249‑1 (MQ=255)
                                           gACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTCT‑CC‑GGTTAACCC‑GGCTTCAATCA‑GTGCCGGATAGAG‑ATTTG‑CCAccgcc                                                                                                                                                                                                  <  1:660810/250‑1 (MQ=255)
                                             cTCCCCCGGCATGAGGCGTACGGAGAGCGTGGTCGAGGGAAGAAACCAGCCACCGGGCGGGGATATCCGGGGTCGGGCGCGGCAATGAAATACTGTGCGGGGGGGGAGGGGCGAACATCCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGTTTAAG‑AG‑AATCAGTTTTTCT‑CC‑GGTTACCCC‑GGCTTCAATCA‑GTGCCGGATAGAG‑ATTTG‑CCACCGCCGt                                                                                                                                                                                                <  2:205619/250‑1 (MQ=255)
                                             cTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAGCCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATCCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTCT‑CC‑GGTTAACCC‑GGCTTCAATCA‑GTGCCGGATAGAG‑ATTTG‑CCACCGCCGt                                                                                                                                                                                                <  1:679730/250‑1 (MQ=255)
                                                 ccccGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTTAG‑AG‑AATCAGTTTTTCT‑TC‑GGGTTACCC‑GGCTTCAATTA‑GTGCCGGACAGAG‑AATTT‑CCACCCCCGTGCCg                                                                                                                                                                                            >  1:24166/1‑250 (MQ=255)
                                                  cccGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AAACAGTTTTTTT‑CC‑GGGTTAACC‑GGCTTCAATCA‑GTGCCGGGTAGAG‑ATTTT‑CCACCCCCGCGCCgg                                                                                                                                                                                           >  1:281961/1‑250 (MQ=255)
                                                         gAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGGTAAG‑AG‑GATTAGTTTTTTT‑CC‑CGTTAAACC‑GGCCTCAAACA‑GTGGCGGCGAGAG‑ATATT‑TCAACCCCGCGCCGgggggacc                                                                                                                                                                                    >  2:607290/1‑246 (MQ=255)
                                                           aGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCCGTTTTTTT‑TC‑GGTTTAACC‑GGCTTTCATTA‑AGGCCGGGTAGAGAATTTG‑CCACCCCCGGCCCGGGGGtacc                                                                                                                                                                                    >  1:704615/1‑246 (MQ=255)
                                                              gTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTTAG‑AG‑AATCCGGTTTTTT‑CC‑GGGTAAACC‑GGCTTCAATCA‑GTGCCGGGTAGAG‑AATTG‑TCACCCCCGTGCCGGGGGGCACAATCa                                                                                                                                                                               >  1:301107/1‑250 (MQ=255)
                                                                        cGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGGTAAG‑AG‑AATCAGGTTTTTT‑CC‑GGGTAACCC‑GGCTTCAATCA‑GTGCCGGATAGAG‑ATTTG‑CCACCGCCGGGCCGGGGGGCACAATCACCGCCAcccg                                                                                                                                                                     >  1:415917/1‑248 (MQ=255)
                                                                         gTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTCT‑CC‑GGTTAACCC‑GGCTTCAATCA‑GCGCCGGATAGAG‑ATTTG‑CCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGgc                                                                                                                                                                    <  1:640785/250‑1 (MQ=255)
                                                                          tgggcggggggaGAAACCAGCGAACGTGCGGGGATATCCGGGGGGGGGCGGGGCAATGAAAGACTGTGCGGGGGGTGAGAGAGAAATATTCCCGGCGGGCGAATTGCCTGATTCGCGCGGCGGTCAATTAGCGCCGGCGGGCGATGGGCGGTTAGG‑AG‑AATCAGTTTTTCT‑CC‑GGTTAACCC‑GGCTTCAATCA‑GTGCCGAATAGAG‑ATTTG‑CCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGgct                                                                                                                                                                   <  2:251297/239‑1 (MQ=255)
                                                                           ggTCGATGGTAGAAACCAGCCACCGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTCT‑CC‑GGTTAACCC‑GGCTTCAATCA‑GTGCCGGATAGAG‑ATTTG‑CCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGgctg                                                                                                                                                                  <  1:213171/250‑1 (MQ=255)
                                                                            gTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTCT‑CC‑GGTTAACCC‑GGCTTCAATCA‑GTGCCGGATAGAG‑ATTTG‑CCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGgctg                                                                                                                                                                  <  1:168630/249‑1 (MQ=255)
                                                                                    tAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTTAG‑AG‑AATTAGTTTTTTT‑TC‑GGGTTACCC‑GGCCTCAATCA‑GTGCCGGATAGAG‑AATTT‑CCACCGCCCTGCCGGAGGTCACAATCACCGCCACCCGCTGCTTTCTGAc                                                                                                                                                         >  1:413378/1‑250 (MQ=255)
                                                                                          ccAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTCT‑CC‑GGTTAACCC‑GGCTTCAATCA‑GTGCCGGATAGAG‑ATTTG‑CCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGc                                                                                                                                                   <  1:625917/250‑1 (MQ=255)
                                                                                                  cGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTTT‑CC‑GGTTAACCC‑GGCTTCAATCA‑GTGCCGGATAGAG‑ATTTG‑CCTCCGCCGTGCCGGGGGGCCCAAACACCGCCACCCGCCGCTTGCTGGCTTTTCCCAAccccc                                                                                                                                           >  1:120987/1‑249 (MQ=255)
                                                                                                          gATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGGTTAG‑AGAAACCGGTTTTTTC‑CC‑GGTTAACCC‑‑GCCTCCATCC‑ATGCCGGGTAGGG‑ATTTT‑CCCCCCCCCTGCCCGGGGGCACCATCACCCCCCCCCGCCGCCTGCTGACTTTCGCCCGcccccccccccgg                                                                                                                                   >  2:322710/1‑241 (MQ=255)
                                                                                                                           gcgGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATGGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTCT‑CC‑GGTTAACCC‑GGCTTCAATCA‑GTGCCGGATAGAG‑ATTTG‑CCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTc                                                                                                                  <  1:545702/250‑1 (MQ=255)
                                                                                                                            cgGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTCT‑CC‑GGTTAACCC‑GGCTTCAATCA‑GTGCCGGATAGAG‑ATTTG‑CCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCa                                                                                                                 <  1:105660/250‑1 (MQ=255)
                                                                                                                            cgGCAATGAAAGGCGGTGCGGGGGGTGAGGGGCGAACATTCCCGGCTGGCAAATTGCCTGATCCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTCT‑CC‑GGTTAACCC‑GGCTTCAATCA‑GTGCCGGATAGAG‑ATTTG‑CCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCa                                                                                                                 <  2:697459/250‑1 (MQ=255)
                                                                                                                                    aaaTACTGTGCGTGGGGTGAGAGGCGACCATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTCT‑CC‑GGTTAACCC‑GGCTTCAATCA‑GTGCCGGATAGAG‑ATTTG‑CCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCG‑AAAt                                                                                                         <  1:458377/249‑1 (MQ=255)
                                                                                                                                    aaaTACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTAAATTAGCTCCGGCGGGCGATCGGCGGTAAAG‑AG‑AATCAGTTTTTCT‑CC‑GGTTAACCC‑GGCTTCAATCA‑GTGCCGGATAGAG‑ATTTG‑CCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCG‑AAATg                                                                                                        <  2:529147/250‑1 (MQ=255)
                                                                                                                                         cTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTCT‑CC‑GGTTAACCC‑GGCTTCAATCA‑GTGCCGGATAGAG‑ATTTG‑CCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCG‑AAATGGgtg                                                                                                    <  1:520763/249‑1 (MQ=255)
                                                                                                                                                    gggagaGGCGAACATTCCCGTCTGGCAATTTGCCTGATTCGCGCCGCAGGAAATTACCCCCGGCGGGCGATCGGCGGTTAAG‑AA‑AATCAGTTTTTCT‑CC‑GGTTACCCC‑GGCTTCAATCA‑GTGCCGGATAGAG‑ATTTG‑CCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCG‑AAATGGGTGTGGTgaatgaat                                                                                        <  2:237983/248‑1 (MQ=255)
                                                                                                                                                               aCATCCCCGGCGGGCGAATTGCCTAATTCGCGCCGCAGTAAATTAGCGCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTCT‑CC‑GGTTACCCC‑GGCTTCAATCA‑GTGCCGGATAGAG‑ATTTG‑CCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCG‑AAATGGGTGTGGTGAATGAATGCGGAATtctc                                                                             <  2:239567/250‑1 (MQ=255)
                                                                                                                                                                      cGGCTGGCGAATTCCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTCT‑CC‑GTTTAACCC‑GGCTTCAACCA‑GTGCCGAATAGAG‑ATTTG‑CCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCG‑AAATGGGTGTGGTGAATGAATGCGGAATTCTccgccgcc                                                                      <  2:320856/250‑1 (MQ=255)
                                                                                                                                                                                 ttGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTCT‑CC‑GGTTAACCC‑GGCTTCAATCA‑GTGCCGGATAGAG‑ATTTG‑CCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTTGCCAGCCCCAGCGCCAGATGCCCCCACCCCCGCTCATCG‑AAAAGGGGGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAA‑CGTTaa                                                          >  1:233363/1‑250 (MQ=255)
                                                                                                                                                                                  tgccgtaTTCCCCCCGCGGTCATTTAGCCCCGGCGGCCGACCGGCGTTTAAG‑AG‑AATCATTTTTTCT‑CC‑GGTTACCCC‑GGCTTCAATCA‑GTGCCGAATAGAG‑TTTCC‑CCCCCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCG‑AAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAA‑CGTTaa                                                          <  2:44621/243‑1 (MQ=255)
                                                                                                                                                                                    ccTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTTT‑CC‑GGTTAACCC‑GGCTTCAATCA‑ATGCCGGATAGAG‑ATTTG‑CCACCGCCCTGCCCGAGGTCACAAACACCGCCCCCGGCTGCTTGCTGGCTTTCGCCCGCCCCCCCCCCCGGAGGCCCCACCCCCCCCCCTCC‑GAATGGGTGTGGTGGATTAATGCGGAATTCCCCCCCGCCCCCAA‑CCTTTAccg                                                       >  2:637203/1‑248 (MQ=255)
                                                                                                                                                                                      tGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTTT‑CC‑GGTTAAAAC‑GGCCTCAAACA‑GTGCCGGGGAAAG‑AATTG‑TCACCCCCGGGGCGGGGGGCACAATAACCGCCACCCGCTGCCTGCTGACTTTCGCCAGCCCCCGCGCCCGAAGCCCCAACCCCCCCGCACCG‑CAATGGGGGGGGGGAATGCAGTCGGGATTCTCCCCCTCCGCTAA‑CGtgtaccgtg                                                     >  2:718010/1‑242 (MQ=255)
                                                                                                                                                                                              gccgcAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTTAG‑AG‑AAACAGTTTTTTTCCC‑GGTTAA‑CC‑GGCCTCCATCA‑GTGCCGGGTAGAG‑ATTTT‑CCCCCCCCGCGCCGGGGGGCACAAACACCGCCCCCCGGCGCTTGCTGACCTTCGCCAGCCCCCACGCCCGATGCCCCAACCCCCCCCCATCG‑AAATGGGGGTGGGGGAAGAAAGCGGGGATCTCCGCCCCCCCTAA‑AGTTTACGgggtgggaaac                                             >  2:698590/1‑243 (MQ=255)
                                                                                                                                                                                                cgcAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTCT‑CC‑GGTTAACCC‑GGCTTCAATCA‑GTGCCGGATAGAG‑ATTTG‑CCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCG‑AAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAA‑CGTTAACGGTGTAGAACGCGa                                           <  1:20265/250‑1 (MQ=255)
                                                                                                                                                                                                     tCAATTAGCTCCGGCGGGCGATCGGCGGTTTAG‑AG‑GATCCGTTTTTTT‑CC‑CCTTAACCC‑GGCTTCAATCA‑GTGTCGGGAAGAG‑ATTTT‑TCCCCCCCCTGTCGGGGGGCACCATTACCCCCACCCGGCCCCTGCCGGCTTTCCCCCGCCCCCACGCCCCATGGCCCCACCCCCCCCCCTCGCAACTGGGGGGGGGGAATAAATCCGGAATT‑TCCGCCCCCCCCAACCCTTAACGGTGAGGAAcgcggcgtg                                       >  2:108119/1‑245 (MQ=255)
                                                                                                                                                                                                      cAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTCT‑CC‑GGGTAAACC‑CGCTTCAATCA‑GTGCCGGATAGAG‑AATTG‑CCACCCCCGTGCCGGCGGGCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCCGCGCCAGATGCCCCAACCCACGCTCATCG‑AAATGGGGGTGGTGAATGAAAGCGGAAATCTCCGCCGCCGCTAA‑CGTTAACGGTGTAGAACGCGAGCCTgg                                     >  1:696743/1‑250 (MQ=255)
                                                                                                                                                                                                       aaTTTGCCCCGGGGGGCCGTCGGGGGGTAAG‑AA‑AAACAGTTTTTCC‑CC‑GGTTAACCC‑GGCTTCAAACC‑GGGCCGGAAAAAG‑AGTTGCCCCCCGCCGTGCCGGAGGTCACAACCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCG‑AAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCACTAA‑CGTTAACGGTGTAGAACGCGAGCCTgg                                     <  2:175101/250‑1 (MQ=255)
                                                                                                                                                                                                           cgccccGGGGGGCGATCGGCGGTTAAG‑AG‑AATCATTTTTTCT‑CC‑GTTTACCCC‑GCCTTCAATCA‑GTCCCGGATAGAG‑ATTTC‑CCCCCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCG‑AAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAA‑CGTTAACGGTGTAGAACGCGAGCCTGGGGCg                                 <  2:415917/245‑1 (MQ=255)
                                                                                                                                                                                                           aGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTTT‑CC‑CGTTAACCC‑CGCCTCAATCA‑GTGGCGGGTAGAG‑ATTTT‑CCACCGCCCTGCCGGGGGGCACAATCACCCCCACCCGCCGCTTTCTGACCTTTGCCCGCCCCCGCGCCAGATGGCCCCACCCCCGCTCCTCG‑CAAAGGGGGTGGGGGATGAATGCGGGAATCTCCCCCCCCCCCAA‑CGGTTACGGGGTAGAACGCGAGGCTGGGGCgg                                >  2:80814/1‑249 (MQ=255)
                                                                                                                                                                                                                   cgggcgccgggcgtTAAAG‑AG‑AATGAGTTTTTGC‑CC‑GTGTACCCC‑GGC‑TCAATTAGGTGCCGAATAGAG‑TTTGC‑CCCCCGCCGTGCCGGAGGTCACAATCCCCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCG‑AAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAA‑CGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGAtg                        <  2:586569/241‑1 (MQ=255)
                                                                                                                                                                                                                                gTTAAG‑AG‑AATCAGTTTTTCT‑CC‑GGTTACCCC‑GGCTTCAATCA‑GTGCCGGATAGAG‑ATTTC‑CCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCG‑AAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAA‑CGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGt           <  1:737650/250‑1 (MQ=255)
                                                                                                                                                                                                                                    ag‑ag‑AATCGTTTTTTCC‑CC‑GGTTAACCC‑GGCTTAAATCA‑GTCCCGGATAGAG‑ATTGG‑CCCCCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCG‑AAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAA‑CGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGAcg       <  2:185003/250‑1 (MQ=255)
                                                                                                                                                                                                                                     g‑ag‑aATCAGTTTTTCT‑CC‑GGTTAACCC‑GGCTTCAATCA‑GTGCCGGATAGAG‑ATTTG‑CCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCG‑AAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAA‑CGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGAcgc      >  2:348376/1‑250 (MQ=255)
                                                                                                                                                                                                                                          aaTCAGTTTTTCT‑CC‑GGTTAACCC‑GGCTTCAATCA‑GTGCCGGATAGAG‑ATTTG‑CCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCG‑AAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAA‑CGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGGGTCTGACGCCGTGACGCGt    >  2:708643/1‑249 (MQ=255)
                                                                                                                                                                                                                                            accatttttCC‑CC‑GGGTCACCCGGGCTCAAACCG‑GCGCCGAATGGAG‑TTTTG‑CCACCGCCGTGCCGGAGGTCCCAATCCCCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCG‑AAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAA‑CGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTa  <  2:18156/246‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       
CCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAG‑AG‑AATCAGTTTTTCT‑CC‑GGTTAACCC‑GGCTTCAATCA‑GTGCCGGATAGAG‑ATTTG‑CCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCG‑AAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAA‑CGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGACGCGTTA  >  NZ_CP009273/2372204‑2372686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑