Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,912,168	G→A	M134I (ATG→ATA)	yebT →	lipid‑binding membrane homeostasis protein YebT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,912,168	0	G	A	100.0%	246.8 / NA	65	M134I (ATG→ATA)	yebT	lipid‑binding membrane homeostasis protein YebT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/26);  total (39/26)

GGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAGGGCGTTGAAGTCGGAACAGTGCAGGATATCAGCCTCAGCGACGATCTTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCATCAATCCCAACAAGCAAGGCGTGGTGATTGATGTCCTGATCGAGCGGCGTTTTACCGATCTGGTGAA  >  NZ_CP009273/1911931‑1912413                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      ggcggATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAGGGCGTTGAAGTCGGAACAGTGCAGGATATCAGCCTCAGCGACGATCTTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGggtaaa                                                                                                                                                                                                                                           <  2:237277/250‑1 (MQ=255)      aTGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAGGGCGTTGAAGTCGGAACAGTGCAGGATATCAGCCTCAGCGACGATCTTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGggtaaaggaa                                                                                                                                                                                                                                       >  1:273222/1‑247 (MQ=255)           aTTGTTCCAGGCCGTACGCCTGTTCGTTATCAGGGCGTTGAAGTCGGAACAGTGCAGGATATCAGCCTCAGCGACGATCTTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAagag                                                                                                                                                                                                                                  >  2:316541/1‑249 (MQ=255)           aTTGTTCCAGGCCGTACGCCTGTTCGTTATCAGGGCGTTGAAGTCGGAACAGTGCAGGATATCAGCCTCAGCGACGATCTTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAagag                                                                                                                                                                                                                                  >  1:519260/1‑249 (MQ=255)                    ggCCGTACGCCTGTTCGTTATCAGGGCGTTGAAGTCGGAACAGTGCAGGATATCAGCCTCAGCGACGATCTTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACt                                                                                                                                                                                                                        >  1:716569/1‑250 (MQ=255)                      ccGTACGCCTGTTCGTTATCAGGGCGTTGAAGTCGGAACAGTGCAGGATATCAGCCTCAGCGACGATCTTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCACAATGCCGGGTAAAGGTAAAGAGCAGGATCACttt                                                                                                                                                                                                                      <  2:641602/250‑1 (MQ=255)                        gTACGCCTGTTCGTTATCAGGGCGTTGAAGTCGGAACAGTGCAGGATATCAGCCTCAGCGACGATCTTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGt                                                                                                                                                                                                                    >  1:227121/1‑250 (MQ=255)                            gCCTGTTCGTTATCAGGGCGTTGAAGTCGACACAGTGCAGGATATCAGCCTCAGCGACGATCGTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCa                                                                                                                                                                                                                <  2:232582/250‑1 (MQ=255)                              cTGTTCGTTATCAGGGCGTTGAAGTCGGAACAGTGCAGGATATCAGCCTCAGCGACGATCTTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACt                                                                                                                                                                                                              >  2:592702/1‑250 (MQ=255)                                     ttATCAGGGCGTTGAAGTCGGAACAGTGCAGGATATCAGCCTCAGCGACGATCTTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATAcc                                                                                                                                                                                                       >  1:97621/1‑250 (MQ=255)                                          aGGGCGTTGAAGTCGGAACAGTGCAGGATATCAGCCTCAGCGACGATCTTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAAcc                                                                                                                                                                                                  <  1:592257/250‑1 (MQ=255)                                               gTTGAAGTCGGAACAGTGCAGGATATCAGCCTCAGCGACGATCTTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGaaa                                                                                                                                                                                              >  1:364696/1‑249 (MQ=255)                                                 tGAAGTCGGAACAGTGCAGGATATCAGCCTCAGCGACGATCTTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCGGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAatat                                                                                                                                                                                           >  2:621901/1‑250 (MQ=255)                                                    aGTCGGAACAGTGCAGGATATCAGCCTCAGCGACGATCTTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCgg                                                                                                                                                                                        >  1:26634/1‑250 (MQ=255)                                                     gTCGGAACAGTGCAGGATATCAGCCTCAGCGACGATCTTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGc                                                                                                                                                                                       >  1:609540/1‑250 (MQ=255)                                                         gAACAGTGCAGGATATCAGCCTCAGCGACGATCTTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGa                                                                                                                                                                                   <  2:138250/250‑1 (MQ=255)                                                           aCAGTGCAGGATATCAGCCTCAGCGACGATCTTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACa                                                                                                                                                                                 <  2:125998/250‑1 (MQ=255)                                                                  aGGATATCAGCCTCAGCGACGATCTTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGa                                                                                                                                                                          >  2:470508/1‑250 (MQ=255)                                                                    gATATCAGCCTCAGCGACGATCTTCGTAAGATTGAAGTCAAGGTCAGCATCAAGGCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATc                                                                                                                                                                        <  1:256543/250‑1 (MQ=255)                                                                       atCAGCCTCAGCGACGATCTTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCtga                                                                                                                                                                     >  1:262015/1‑250 (MQ=255)                                                                        tCAGCCTCAGCGACGATCTTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCtgat                                                                                                                                                                    >  2:597234/1‑250 (MQ=255)                                                                               cAGCGACGATCTTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCAc                                                                                                                                                             >  1:234989/1‑250 (MQ=255)                                                                                                    tGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGt                                                                                                                                        >  2:681918/1‑250 (MQ=255)                                                                                                     gAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGAAAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGtt                                                                                                                                       >  2:604601/1‑250 (MQ=255)                                                                                                        gTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGc                                                                                                                                    <  2:451769/250‑1 (MQ=255)                                                                                                         tCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCt                                                                                                                                   >  2:267774/1‑250 (MQ=255)                                                                                                             ggTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATc                                                                                                                                                                                                                           >  2:501653/1‑158 (MQ=255)                                                                                                              gTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCa                                                                                                                                                                                                                          <  1:501653/158‑1 (MQ=255)                                                                                                                     tCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGtt                                                                                                                        >  1:318626/1‑249 (MQ=255)                                                                                                                            cGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCCTAATGCCGGGGAAAGGCAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGGCAATGGCGAGGTGATGAACCCCCCGCACACCCCGGTTCTCCGTTCGCCGAACAGCGGTACATTgggc                                                                                                                >  2:714546/1‑248 (MQ=255)                                                                                                                            cGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTc                                                                                                                >  2:201618/1‑250 (MQ=255)                                                                                                                             gATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTCTATCGGCATAATGCCGGGTAAAGGAAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGAAAATGGCGATCTGATGATCCACCTGCAAGCCACCGATCTCGGATCGCTGAACAGCGGTTCATTGGTc                                                                                                                >  1:461972/1‑249 (MQ=255)                                                                                                                              atatGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGCTCTGGGGTCGCTGCACAGCGGTTCCTTGGTCTa                                                                                                              >  2:269979/1‑250 (MQ=255)                                                                                                                                   aaaGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTcc                                                                                                         <  2:670466/250‑1 (MQ=255)                                                                                                                                    aaGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCg                                                                                                        <  1:247276/250‑1 (MQ=255)                                                                                                                                    aaGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCg                                                                                                        >  1:649631/1‑250 (MQ=255)                                                                                                                                          cgctgcgcGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGc                                                                                                       >  1:375817/1‑245 (MQ=255)                                                                                                                                          cgctgcgcGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGAt                                                                                                  <  1:320101/250‑1 (MQ=255)                                                                                                                                                gcgAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGAAAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGt                                                                                            >  2:82832/1‑250 (MQ=255)                                                                                                                                                     gagaCTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGaa                                                                                       <  2:522615/250‑1 (MQ=255)                                                                                                                                                     gagaCTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGa                                                                                        >  2:240503/1‑249 (MQ=255)                                                                                                                                                        aCTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAg                                                                                    >  1:82280/1‑250 (MQ=255)                                                                                                                                                            aGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTc                                                                                  <  2:609540/248‑1 (MQ=255)                                                                                                                                                                 tggctggTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGGGGGAAAAGTCTACGAc                                                                            >  2:421947/1‑249 (MQ=255)                                                                                                                                                                   gctggTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTa                                                                          <  1:351914/249‑1 (MQ=255)                                                                                                                                                                          aCGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCATCa                                                                  <  2:33482/250‑1 (MQ=255)                                                                                                                                                                            gCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCATCAAt                                                                >  2:280220/1‑250 (MQ=255)                                                                                                                                                                            gCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCATCAAt                                                                >  2:51076/1‑250 (MQ=255)                                                                                                                                                                             ccAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCATCAATc                                                               >  2:735414/1‑250 (MQ=255)                                                                                                                                                                              cAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCTGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCGCCGACTATGCCCTCCATcc                                                              >  2:451152/1‑250 (MQ=255)                                                                                                                                                                                 aaGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCATCAATCCcaa                                                           <  2:310746/250‑1 (MQ=255)                                                                                                                                                                                   gCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCATCAATCCcaaca                                                         <  1:267774/250‑1 (MQ=255)                                                                                                                                                                                    cATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCATCAATCCcaacaa                                                        <  2:624546/250‑1 (MQ=255)                                                                                                                                                                                                    ctcCGGGCTGGACGCCCTCGTCTGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCATCAATCCCAACAAGCAAGGCGTGGtgatt                                        <  2:630944/250‑1 (MQ=255)                                                                                                                                                                                                       cGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCATCAATCCCAACAAGCAAGGCGTGGtgattga                                      <  1:316541/249‑1 (MQ=255)                                                                                                                                                                                                              gACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCATCAATCCCAACAAGCAAGGCGTGGTGATTGATGTCCTg                               <  1:512547/249‑1 (MQ=255)                                                                                                                                                                                                              gACGCCCTCGTCGGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCATCAATCCCAACAAGCAAGGCGTGGTGATTGATGTCCTGa                              <  2:262015/250‑1 (MQ=255)                                                                                                                                                                                                                       gtcgGTGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCATCAATCCCAACAAGCAAGGCGTGGTGATTGATGTCCTGATCGAgcg                       <  2:481123/248‑1 (MQ=255)                                                                                                                                                                                                                            tGGGAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCATCAATCCCAACAAGCAAGGCGTGGTGATTGATGTCCTGATCGAGCGGCGttt                 >  2:452123/1‑249 (MQ=255)                                                                                                                                                                                                                              ggAACTATATCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCATCAATCCCAACAAGCAAGGCGTGGTGATTGATGTCCTGATCGAGCGGCGTTTTAc              >  2:507995/1‑250 (MQ=255)                                                                                                                                                                                                                                   tataTCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCATCAATCCCAACAAGTAAGGCGTGGTGATTGATGTCCTGATCGAGCGGCGTTTTACCGATc         >  2:202742/1‑250 (MQ=255)                                                                                                                                                                                                                                     tatCGGCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCATCAATCCCAACAAGCAAGGCGTGGTGATTGATGTCCTGATCGAGCGGCGTTTTACCGATCTg       <  2:67918/250‑1 (MQ=255)                                                                                                                                                                                                                                         ggCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCATCAATCCCAACAAGCAAGGCGTGGTGATTGATGTCCTGATCGAGCGGCGTTTTACCGATCTGGTGa   <  2:227121/250‑1 (MQ=255)                                                                                                                                                                                                                                          gCATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCATCAATCCCAACAAGCAAGGCGTGGTGATTGATGTCCTGATCGAGCGGCGTTTTACCGATCTGGTGa   >  2:16036/1‑249 (MQ=255)                                                                                                                                                                                                                                           cATAATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCATCAATCCCAACAAGCAAGGCGTGGTGATTGATGTCCTGATCGAGCGGCGTTTTACCGATCTGGTGaa  >  2:242542/1‑249 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      GGCGGATGGTATTGTTCCAGGCCGTACGCCTGTTCGTTATCAGGGCGTTGAAGTCGGAACAGTGCAGGATATCAGCCTCAGCGACGATCTTCGTAAGATTGAAGTCAAGGTCAGCATCAAGTCCGATATGAAAGATGCGCTGCGCGAAGAGACTCAGTTCTGGCTGGTGACGCCAAAAGCATCGTTGGCAGGTGTCTCCGGGCTGGACGCCCTCGTCGGTGGGAACTATATCGGCATGATGCCGGGTAAAGGTAAAGAGCAGGATCACTTTGTCGCACTCGATACCCAACCGAAATATCGGCTGGACAATGGCGATCTGATGATCCACCTGCAAGCCCCCGATCTCGGTTCGCTGAACAGCGGTTCATTGGTCTATTTCCGCAAGATCCCGGTGGGAAAAGTCTACGACTATGCCATCAATCCCAACAAGCAAGGCGTGGTGATTGATGTCCTGATCGAGCGGCGTTTTACCGATCTGGTGAA  >  NZ_CP009273/1911931‑1912413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑