Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,671,3980TG35.2% 136.2 / 21.3 71N108T (AAC→ACC) yphEsugar ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (7/39);  new base G (24/1);  total (31/40)
Fisher's exact test for biased strand distribution p-value = 1.06e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGATCACCAGTTCAACTTCCGCACTCGCAAGCGAACTGGTAGGTTCATCAAGAATGACCACGCGCGGCTCGCCCTTCATCACCCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGA‑ATGAGAGTCGATTTGCCCGCG‑CCGTTTTTACCTAACAGCGCACGAACTTCGCCTTTATTGAGCGTGAAGTTAACGTTAT  >  NZ_CP009273/2671153‑2671641
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     
tGATCACCAGTTCAACTTCCGCACTCGCAAGCGAACTGGTAGGTTCATCAAGAATGACCACGCGCGGCTCG‑CCTTCATCACCCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCCGACCCCGGCAGGGGGGTTcc                                                                                                                                                                                                                                                  >  2:265140/1‑250 (MQ=255)
     cccAGTTCAACTTCCGCACTCGCAAGCGAACTGGTAGGTTCATCAAGAATGACCACGCGCGGCTCGCCCTTCATCACCCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCTGGGCCACTGACCGAGGCAGAGGTTTTCCGCCa                                                                                                                                                                                                                                              <  2:916655/249‑1 (MQ=255)
      ccAGTTCAACTTCCGCACTCGCAAGCGAACTGGTAGGTTCATCAAGAATGACCACGCGCGGCTCGCCCTTCATCACCCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCAc                                                                                                                                                                                                                                             <  2:406439/250‑1 (MQ=255)
       cAGTTCAACTTCCGCACTCGCAAGCGAACTGGTAGGTTCATCAAGAATGACCACGCGCGGCTCGCCCTTCATCACCCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCACCCACCGAGGGAGGAGGTTTCCGCCAcc                                                                                                                                                                                                                                            >  2:349654/1‑249 (MQ=255)
         gTTCAACTTCCGCACTCGCAAGCGAACTGGTAGGTTCATCAAGAATGACCACGCGCGGCTCGCCCTTCATCACCCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGt                                                                                                                                                                                                                                          <  1:476403/250‑1 (MQ=255)
            cAACTTCCGCACTCGCAAGCGAACTGGTAGGTTCATCAAGAATGACCACGCGCGGCTCGCCCTTCATCACCCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCa                                                                                                                                                                                                                                        <  1:182301/249‑1 (MQ=255)
             aaCTTCCGCACTCGCAAGCGAACTGGTAGGTTCATCAAGAATGACCACGCGCGGCTCGCCCTTCATCACCCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGTGGCCCTCACCCAGGGCGAGGTGTTCCGCCCCCGTCCGc                                                                                                                                                                                                                                      >  2:935710/1‑250 (MQ=255)
             aaCTTCCGCACTCGCAAGCGAACTGGTAGGTTCATCAAGAATGACCACGCGCGGCTCGCCCTTCATCACCCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGc                                                                                                                                                                                                                                      <  1:873897/250‑1 (MQ=255)
                  ccGCACTCGCAAGCGAACTGGTAGGTTCATCAAGAATGACCACGCGCGGCTCGCCCTTCATCACCCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCtt                                                                                                                                                                                                                                  <  1:695216/249‑1 (MQ=255)
                  ccGCACTCGCAAGCGAACTGGTAGGTTCATCAAGAATGACCACGCGCGGCTCGCCCTTCATCACCCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACAGCCCTAGGGAGAGGGTTTCCGCCCCCGTCCGCCCTTc                                                                                                                                                                                                                                 >  1:703590/1‑250 (MQ=255)
                    gCACTCGCAAGCGAACTGGTAGGTTCATCAAGAATGACCACGCGCGGCTCGCCCTTCATCACCCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCTGACCGGGGCAGGGGGTTTCCGCCACTGTCAGCCCCTccc                                                                                                                                                                                                                               >  2:397171/1‑249 (MQ=255)
                          gCAAGCGAACTGGTAGGTTCATCAAGAATGACCACGCGCGGCTCGCCCTTCATCACCCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGa                                                                                                                                                                                                                          <  1:716864/249‑1 (MQ=255)
                              gCGAACTGGTAGGTTCATCAAGAATGACCACGCGCGGCTCGCCCTTCATCACCCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCCAGGCAGAGGGTTTTCGCCACCGTCAGCCCCTCCCCCacacctta                                                                                                                                                                                                                     >  1:121701/1‑246 (MQ=255)
                                 aaCTGGTAGGTTCATCAAGAATGACCACGCGCGGCTCGCCCTTCATCACCCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACCGCGGCAGAGGGTTTTTGCCCCCCTCGACCCTTccacccaccctaactc                                                                                                                                                                                                                  >  2:713091/1‑239 (MQ=255)
                                   cTGGTAGGTTCATCAAGAATGACCACGCGCGGCTCGCCCTTCATCCCCCGCGCAATTTCCCCCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCt                                                                                                                                                                                                                <  2:361065/250‑1 (MQ=255)
                                    tGGTAGGTTCATCAAGAATGACCACGCGCGGCTCGCCCTTCATCACCCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACCCAGGCCGAGGGTTTTCGCCCCTGTCAGCCCTTCCACCCGACTTAACTCCt                                                                                                                                                                                                                >  1:559837/1‑249 (MQ=255)
                                    gggTAGGTGCAGCAAGAATGACCCCGCGCGGCTCGCCCTTCATCCCCCGCGCAATTCCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGGTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGGTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTg                                                                                                                                                                                                               <  2:252064/249‑1 (MQ=255)
                                                    aaTGACCACGCGCGGCTCGCCCTTCATCACCCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGGCCGCGGCAGAGGGTTTCCGCCCCCGTCAGCCCCTCCACCCGACTTTACTCCTTATAAACCCCGCCCAcc                                                                                                                                                                                               >  1:13103/1‑250 (MQ=255)
                                                     aTGACCACGCGCGGCTCGCCCTTCATCACCCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCTACCGCGGCGGAGGGTGTCCGCCACAGTCAGCCCCCCCCCCCCACTCACCTCCTGTTAAACCccccgccacc                                                                                                                                                                                              >  2:831724/1‑246 (MQ=255)
                                                     aTGACCACGCGCGGCTCGCCCTTCATCACCCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGGCCGAGGCAGAGGGTTTTCGCCCCTGTCAGCCCTTCCACCAGACTTAAATCCTGATAAACCCCGCGCAccc                                                                                                                                                                                              >  1:421693/1‑250 (MQ=255)
                                                         ccACGCGCGGCTCGCCCTTCATCACCCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAg                                                                                                                                                                                          <  1:771359/250‑1 (MQ=255)
                                                            cgcgcgGCTCGCCCTTCATCACCCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTc                                                                                                                                                                                       <  1:720497/250‑1 (MQ=255)
                                                             gcgcgGCTCGCCCTTCATCACCCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTCCCCGGGGCACAGGTTTTTCGGCACTGTCAGCCCTTCCACCAGACTTAAATCCTGATAAACCGCGCGCACCCCCCGTTcc                                                                                                                                                                                      >  2:536630/1‑249 (MQ=255)
                                                                 ggCTCGCCCTTCATCACCCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCTGACCGAGGCCGAGGGTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCCGATAAACCCCGCGCACCCCCCGCTCAGCgg                                                                                                                                                                                  >  1:885348/1‑250 (MQ=255)
                                                                 ggCTCGCCCTTCATCACCCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACCCGGGCGGGGGGTTTCCCCCCCTCCCGGCCCCTCCCCCCCACTTAACTCCTGGTAAAACACCGCCCCCCCCACTTCACCgg                                                                                                                                                                                  >  2:555412/1‑250 (MQ=255)
                                                                    tCGCCCTTCATCACCCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCCCGGCAGCGGGTTTCCGCCCCCGTCCGCCCCTCCCCCAGACCTCAATCCTGATAAAAACCGCGCTCCCCCCGTTCATCGGCaa                                                                                                                                                                               >  1:722441/1‑249 (MQ=255)
                                                                    tCGCCCTTCATCACCCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACCGAGGCAGGGGGTTTCCGCCACCGTCAGCCCCTTCACCAGACTTTACCCCTGATAAAACGCCCGCACCCCCCGTTCAGCCGCAc                                                                                                                                                                               >  1:193549/1‑250 (MQ=255)
                                                                               caCCCGCGCAATCTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTTCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGGTAAACCGCGCGCACCCCCACTTCCGCGGCCCCGCGAGTCCGc                                                                                                                                                                    >  1:398919/1‑250 (MQ=255)
                                                                                                    ctgctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGAAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGt                                                                                                                                               <  1:448314/250‑1 (MQ=255)
                                                                                                          tggcgcTGGGCTGAGCGTTGAAACAATTTGTTCAGGCCTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCTTTTGCAGGTAGTCAATCATGCCGTGGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGt                                                                                                                                          <  2:632741/247‑1 (MQ=255)
                                                                                                           tGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGGCCGAGGGAGAGGGTTTTCCCCCCCGTCCGACCTTCCCCCCCCCCTAACTCCTGGTTAAACCCGCGCCCCCCCCGTTCAGCGGCCGCGCCCGGCCGCGCAGCTTCCTCCCCCTCCCATCGCGtcc                                                                                                                                        >  2:105762/1‑249 (MQ=255)
                                                                                                             cgcCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACCCCGGCAGGGGTTTTTCTCCCCCTTCCGCCCTTCCCCCAGCCCTAACTCCTGCTAAACCCCGCGCCCCCCCCCTTCCTCGGCCCCGCGGGCCACGGCTGCCTCGTCCCCTTCCCGTCGCCtctcc                                                                                                                                      >  2:911019/1‑249 (MQ=255)
                                                                                                              gcCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGCCCGAGGCAGAGGGGTTTCCCCCCTGTTCGGCCTTCCACCAGACTCTACTCCTGATAAAACCCGCGCCCCCCCCGACCCCCGGCCCGGCGAGGCAGCGCAAACTCGGCACCCtcccgtcccggccccc                                                                                                                                     >  2:827803/1‑244 (MQ=255)
                                                                                                                  ggCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAAt                                                                                                                                 <  1:470561/250‑1 (MQ=255)
                                                                                                                  ggCTTAGCGTGAAAACAAGTTGTGCAGGACAAACTCCAACGCCCAGCGCCTGTAACAAACGTGGGGCCTCTGGCGCCATTTGAAGGTAATCAACCATGCCGTGGCGGCGGGGCCACGGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAAt                                                                                                                                 <  2:747815/250‑1 (MQ=255)
                                                                                                                       aGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGCGGCAGAGGGTTTTCGCCCCCGTCAGCCCCTCCACCAGACTTAACTCCTGATAAAACCCCCCCCCCCCCCCTTCACCGGCACGGCGAGGCAGCGGTACCTCCTCACCCTCCCCTCGCCGCTCGcccaacccca                                                                                                                            >  2:252981/1‑242 (MQ=255)
                                                                                                                       aGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCa                                                                                                                              <  1:626823/248‑1 (MQ=255)
                                                                                                                                       gttCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACCCAGGCAGAGGGTTTTCGCCCCCGTCAGCCCCTCCCCCCCACTTAACTCCTGCTAAAACGCGCCCACCCCCCACTCCGCGGCCCGGCGAGGCAGCGGAGCCTCGTCACCCTCCACCCCCGGCTCGCCCACCCCGCAATAACCGCCATcccc                                                                                                            >  2:965043/1‑248 (MQ=255)
                                                                                                                                       gttCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCg                                                                                                             <  1:474846/249‑1 (MQ=255)
                                                                                                                                        ttCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCggg                                                                                                           <  1:530612/250‑1 (MQ=255)
                                                                                                                                                     tCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCgg                                                                                              <  1:154852/250‑1 (MQ=255)
                                                                                                                                                     tCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCgg                                                                                              <  1:902790/250‑1 (MQ=255)
                                                                                                                                                       aaCGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGt                                                                                             <  1:589949/249‑1 (MQ=255)
                                                                                                                                                       aaCGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTa                                                                                            <  1:935710/250‑1 (MQ=255)
                                                                                                                                                             cAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCACCCACCCAGGCCGAGGGTTTTTGCCCCTTTCAGCCCCTCCCCCCAACATTACTCCTGATAAACCGCCCGCACCCCCCCTTCAGCGGCCCCGCGAGGCAACGGAGCTTCGTCCCCCTCCCATCGCGGCTTCCCCACCCAGAAACTCCCCCAATCCGGCCGTTCCCCGCCgggcagcctt                                                                                      >  2:796351/1‑242 (MQ=255)
                                                                                                                                                                          cAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGA‑ATGAGAGTCg                                                                        <  1:766171/250‑1 (MQ=255)
                                                                                                                                                                           aaCGTTGGGCATCCTGCGCCGTTGTCAGGTAATCAATCATGCCGGTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGA‑ATGAGAGTCGa                                                                       <  1:168295/250‑1 (MQ=255)
                                                                                                                                                                           aaCGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGTGCCACTCACCCGGGCAGAGGGTTTTCGCCACTGTCGGTCCTTCCACCCGTCCTAACTACCGCTAAAACCCGCGCACCCCCCCGTTATCGGCACCTCGCGGCCATGGAGATTCTTCACCTTCCAGTCGCCGCTCCGCAATCCCGATATTACCCCACTCCCGGGGGTCGCCGCCCGTGAGGACTCGA‑ATGAGAGGCGa                                                                       >  2:397406/1‑250 (MQ=255)
                                                                                                                                                                             cGTGGGGCACCCTGCGCCATTTGCAGGTAATCAATCACGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGA‑ATGAGAGTCGAtt                                                                     <  2:289975/250‑1 (MQ=255)
                                                                                                                                                                                    cgtCCTGCGCCATTTGCAGTTAAGCAAGCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGA‑ATGAGAGTCGATTTGCCcgc                                                              <  2:193549/248‑1 (MQ=255)
                                                                                                                                                                                       ccTGCGCCATTTGCAGGTAACCAATCAGGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGA‑ATGAGAGTCGATTTGCCCGCG‑cc                                                          <  2:227701/250‑1 (MQ=255)
                                                                                                                                                                                         tGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGCCCGAGGAAGAGGGGTTTCGCCACCGTTAGCCCCTCCCCCCCACTTAACTCCCGCTAAAACGCGCCCACCCCCCCCTCAGCGGCCCCGCGAGGCAGCGCAGCCTCCTCCCCCTCCCGTCGCGTCCCGCCCATCCCCATATCACCCCTCTCCCGGCGGTCCCTGCCCGTCAGCCTTTCA‑CTGAGAGCCGATTTTCCCGcc‑cccc                                                        >  2:626368/1‑248 (MQ=255)
                                                                                                                                                                                            cgcATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGA‑ATGAGAGTCGATTTGCCCGCG‑CCGtt                                                       <  1:7234/246‑1 (MQ=255)
                                                                                                                                                                                             ccATTGGCAGTAAATCAATCATGCCGTTGCGGCGGGCCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGA‑ATGAGAGTCGATTTGCCCGCG‑CCGttttt                                                    <  2:402414/250‑1 (MQ=255)
                                                                                                                                                                                               aTTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGCGGGAGAGGGTTTTCGCCACTGTCAGCCCCTCCACCAGACTTAACTCCTGGTAAACCGCGCCCACCCCCCCCTCCGCGGCACGGCGAGGCAGCGGAGCTTCCTCACCCTCCACTCGCGTCCCCCCCATCCACATATCACCGCTCTCCCGGCGTGCGCTGCCGGGCAGCCTTCGA‑CTGAGGGGAGATTTGCCCGCGCCCGTTTTTa                                                   >  2:90080/1‑250 (MQ=255)
                                                                                                                                                                                                tttGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACCGCGGGGGAGGGTTTTCGCCCCCGTCAGCCCCTCCACCCGACTTAAATCCTTATAAAACCCGCGCACCCCCCCCTCAGCGGCGCGGCGAGGCAGCGTAGGCTCGTCACCCTCCCGTCGCGGCCCGCCCAACCCCGTATAACCCCCATCCCGGGGTGCGCCGGCGGGAAGGACTCGACATG‑GAGTGGATTTTTCCGCG‑cccggtttttc                                                 >  2:191058/1‑241 (MQ=255)
                                                                                                                                                                                                   gCAGGTATGCATTCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGA‑ATGAGAGTCGATTTGCCCGCG‑CCGTTTTTACCTaa                                              <  2:230340/250‑1 (MQ=255)
                                                                                                                                                                                                    cAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGAACGCGGGAGAGGGTTTTCGCCCCTGTCCGCCCCTCCCCCCGACTTAACTCCTGATAAAACGCGCGCCCCCCCACTTCCGCGGCACCGCGGGGCAGCGTAGCTTCGTCCCCCTCCACTCGCGGCCCGCCCAACCCGATATCACCGCTATCCCGGCGGTCGCTGCCGGGTAGCATTCGT‑ATGAGGGGCGGTTTTCCCGCC‑CCGGTTTTTCCtta                                              >  2:915790/1‑247 (MQ=255)
                                                                                                                                                                                                       ttAATCAATCAGGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGA‑ATGAGAGTCGATTTGCCCGCG‑CCGTTTTTACCTAACAgc                                          <  1:542470/249‑1 (MQ=255)
                                                                                                                                                                                                       gaaatcaagcATGCCGTTGCGGCGGGGCCACTGACCGGGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGA‑ATGAGAGTCGATTTGCCCGCG‑CCGTTTTTACCTAACAgc                                          <  2:634424/248‑1 (MQ=255)
                                                                                                                                                                                                          atcaatcaTGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGA‑ATGAGAGTCGATTTGCCCGCG‑CCGTTTTTACCTAACAGCGCa                                       <  1:61314/250‑1 (MQ=255)
                                                                                                                                                                                                                  tGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGA‑ATGAGAGTCGATTTGCCCGCG‑CCGTTTTTACCTAACAGCGCACGAACTTc                               <  1:34828/250‑1 (MQ=255)
                                                                                                                                                                                                                          cggcggGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCGTTCGA‑ATGAGAGTCGATTTGCCCGCG‑CCGTTTTTACCTAACAGCGCACGAACTTCGCCTTTAt                       >  1:61031/1‑250 (MQ=255)
                                                                                                                                                                                                                           ggcggGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGA‑ATGAGAGTCGATTTGCCCGCG‑CCGTTTTTACCTAACAGCGCACGAACTTCGCCTTTAtt                      <  1:831917/250‑1 (MQ=255)
                                                                                                                                                                                                                             ggggcCCCCTGCCCGAGGCAGGTGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGA‑ATGAGAGTCGATTTGCCCGCG‑CCGTTTTTACCTAACAGCGCACGAACTTCGCCTTTATTGa                    <  2:21835/249‑1 (MQ=255)
                                                                                                                                                                                                                              ggggCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGA‑ATGAGAGTCGATTTGCCCGCG‑CCGTTTTTACCTAACAGCGCACGAACTTCGCCTTTATTGAg                   <  1:661798/250‑1 (MQ=255)
                                                                                                                                                                                                                                    aCTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGA‑ATGAGAGTCGATTTGCCCGCG‑CCGTTTTTACCTAACAGCGCACGAACTTCGCCTTTATTGAGCGTGaa             <  1:228153/250‑1 (MQ=255)
                                                                                                                                                                                                                                        aCCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGA‑ATGAGAGTCGATTTGCCCGCG‑CCGTTTTTACCTAACAGCGCACGAACTTCGCCTTTATTGAGCGTGAAGt           <  1:265140/248‑1 (MQ=255)
                                                                                                                                                                                                                                         ccGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGA‑ATGAGAGTCGATTTGCCCGCG‑CCGTTTTTACCTAACAGCGCACGAACTTCGCCTTTATTGAGCGTGAAGTTaa        >  1:462856/1‑250 (MQ=255)
                                                                                                                                                                                                                                           gAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGA‑ATGAGAGTCGATTTGCCCGCG‑CCGTTTTTACCTAACAGCGCACGAACTTCGCCTTTATTGAGCGTGAAGTTAACg      >  1:514087/1‑250 (MQ=255)
                                                                                                                                                                                                                                               cAGGGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGAGAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGA‑ATGAGAGTCGATTTGCCCGCG‑CCGTTTTTACCTAACAGCGCACGAACTTCGCCTTTATTGAGCGTGAAGTTAACGTTAt  <  2:670921/250‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     
TGATCACCAGTTCAACTTCCGCACTCGCAAGCGAACTGGTAGGTTCATCAAGAATGACCACGCGCGGCTCGCCCTTCATCACCCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCGGTAAGCATTCGA‑ATGAGAGTCGATTTGCCCGCG‑CCGTTTTTACCTAACAGCGCACGAACTTCGCCTTTATTGAGCGTGAAGTTAACGTTAT  >  NZ_CP009273/2671153‑2671641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑