Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,977,800	0	A	C	35.2%	144.6 / 26.5	71	G384G (GGT→GGG)	yqeF	acetyl‑CoA C‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (41/5);  new base C (0/25);  total (41/30)
Fisher's exact test for biased strand distribution p-value = 1.43e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TAAAGGGATGATATGCCCATGGGCTAGGGTTTTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCC  >  NZ_CP009273/2977553‑2978042                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   taAAGGGATGATATGCCCATGGGCTAGGGTTTTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACAcc                                                                                                                                                                                                                                                  >  2:592631/1‑250 (MQ=255) taAAGGGATGATATGCCCATGGGCTAGGGTTTTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACAcc                                                                                                                                                                                                                                                  >  1:244033/1‑250 (MQ=255) taAAGGGATGATATGCCCATGGGCTAGGGTTTTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACAcc                                                                                                                                                                                                                                                  >  1:495303/1‑250 (MQ=255)    aGGGATGATATGCCCATGGGCTAGGGTTTTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTg                                                                                                                                                                                                                                               <  1:14669/250‑1 (MQ=255)     gggATGATATGCCCATGGGCTAGGGTTTTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTgg                                                                                                                                                                                                                                              <  1:360921/250‑1 (MQ=255)      ggATGATATGCCCATGGGCTAGGGTTTTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGc                                                                                                                                                                                                                                             <  2:654143/250‑1 (MQ=255)           atatGCCCATGGGCTAGGGTTTTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGccccc                                                                                                                                                                                                                                         <  1:101752/249‑1 (MQ=255)              tGCCCATGGGCTAGGGTTTTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGCCCCccgcc                                                                                                                                                                                                                                      >  2:662447/1‑249 (MQ=255)                cccATGGGCTAGGGTTTTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGAAGGAAATGCAGAGCTATTAGTCACGTTCAATGGTCAATGCCACACCCTGGCCCccgccgc                                                                                                                                                                                                                                    >  2:693218/1‑248 (MQ=255)                       tcTAGGTTTTTAAATAAAAAAAAAAACAATGTTTCATTTTTAAGTTAAGGATAAAAAAAAGTGCAGTGTCGAAAACGAATATGTCAGGAGAATGAAAAAAGGAGGGAGCCAAAAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATTTTTATCGGTAAGGAGAAAATGCAGAGCTATTCGTCACGTTCAATGGTCAACGCCCCCCCCTGGCCCCCGCCGATACAAAGc                                                                                                                                                                                                                            <  2:329664/249‑1 (MQ=255)                                ttCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGt                                                                                                                                                                                                                   >  1:302858/1‑250 (MQ=255)                                 taaaTTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGAATTAAAAAAAGTGCAGTGTCGATCACAAATATGTAATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATAAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTAAATGCCACCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGt                                                                                                                                                                                                                   <  1:414135/247‑1 (MQ=255)                                   aaTTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCt                                                                                                                                                                                                                >  2:802772/1‑250 (MQ=255)                                        aaaaTAAAAAAATGTTTAATTTTAAAGTAAAGAATAAAAAAAAGGGCAGTGTCGATCACGAATATGTCAAGAGAATGAAAAAAGGAGGGAGCCAAAAAAATGTATGTCGACTTGCCAGCAATCATTATTAAACAGATAAAGGACGGCAGATATTTAGCGGAAAGGAGAAAAGGCAGAGCTTTTCGTCAGGTTAAAGGGTAAACCCCACCCCCGGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCg                                                                                                                                                                                                           <  2:320236/250‑1 (MQ=255)                                         aaaTAAAACAATGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCgg                                                                                                                                                                                                          >  1:879510/1‑250 (MQ=255)                                                  aaTGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGt                                                                                                                                                                                                 >  2:179968/1‑250 (MQ=255)                                                       ttCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCAc                                                                                                                                                                                            >  2:697576/1‑250 (MQ=255)                                                           tttttAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCAtt                                                                                                                                                                                        >  2:683622/1‑250 (MQ=255)                                                              ttaagttaagGATTAAAAAAAGTGCAGTGTCAATCACAAATATGTCATGAAAATGAAAAAATGAGGGAGCCCAGAAAATTTATGTCGCCTTGCCAGCCATCATTATTCAACATATTAATGACGGCAGATATTTATCGGTAAGGAGAAAATGCAGAGCTTTTCGTCACGTTCAATGGTCAATCCCCCCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTc                                                                                                                                                                                      <  1:420006/249‑1 (MQ=255)                                                              taaagaaaagAATAAAAAAAGGTGAGGTGTCGAAAAGAAATAGGTCATGAAAATAAAAAAAGGAGGGACCACAAAAAGTGAATGGCGTTTTGCCACCAATCATTATCAAAAAGATAAAGGCGGGAAGATATTTAGGGGAAAGGAAAAAATGCAGAGCTATCCGTCCGTTTAAATGTTCAACCCCCCCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTc                                                                                                                                                                                      <  2:280571/242‑1 (MQ=255)                                                                aaattaagaaaaaaaaaaaGGGCAGTGTAGATCAAGAATATGTCATGAGAATAGAAAAAGGAGGGACCCCAGAAAAGGTAGTCCGACTGGCCAGCCATTATTATAAAAAAGATTAAGGACGGAAGATTTTTACGGAGAGGGAGAAAATGCGGAGCTTTGCGTCACGTTAAATGGTAATTGCCCCCCCGGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATg                                                                                                                                                                                   <  2:947172/238‑1 (MQ=255)                                                                             aaaaaaGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAAc                                                                                                                                                                       >  2:504355/1‑249 (MQ=255)                                                                             aaaaaaGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGAATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGCCATTACGTTTCCCCATTTCATGAACCAGAGAAAc                                                                                                                                                                       >  2:531465/1‑249 (MQ=255)                                                                                     gCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGCTTCg                                                                                                                                                              >  1:365518/1‑250 (MQ=255)                                                                                     gCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCg                                                                                                                                                              >  1:600586/1‑250 (MQ=255)                                                                                         tgtCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCaa                                                                                                                                                          >  1:493782/1‑250 (MQ=255)                                                                                                cACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGAATTCGGCAACCGGAAg                                                                                                                                                   >  1:70973/1‑250 (MQ=255)                                                                                                aaagAATAGGTAATGAGAATGAAAAAATGAGGGGACACAAAAAATTTATTTCGGCTGGCCACCCATCTTTATTAAAAAAATAAATGCGGGCAATTTTTTATCGGAAGGGAGAAAATGCAGAGTTTTTCGTCGCGTTCATTGGTCAAGCCCCCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAg                                                                                                                                                   <  2:727099/247‑1 (MQ=255)                                                                                                  cGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAgcg                                                                                                                                                 >  1:784060/1‑250 (MQ=255)                                                                                                                          aTGAGGGAGCCCAAAAAATGTATGTCGACTTGCCAGCCATCATTATTCAAAAGATTAATGACGGCAGATATTTATCGGAAAGGAGAAAATGCAGAGTTTTTCGTCACTTTAAATGGTAAATCCCACCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATc                                                                                                                         <  1:29783/250‑1 (MQ=255)                                                                                                                            gAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATcgc                                                                                                                       >  2:188854/1‑250 (MQ=255)                                                                                                                                       aaaatttgtggtCGACTTCCCAGCATTAATTATAAAACAGATTAAGGACGGAAATTTTTTAGGGGTAAGAAGAAAATGCAAATTTTTTCGTCCGGTTAAAGGGCCAACCCCCCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACcattga                                                                                                            <  2:274518/240‑1 (MQ=255)                                                                                                                                          aaTGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACcattgacat                                                                                                         >  2:117845/1‑250 (MQ=255)                                                                                                                                                 gtCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCattgacattgacac                                                                                                   >  2:556359/1‑247 (MQ=255)                                                                                                                                                    gACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACg                                                                                               >  1:891640/1‑250 (MQ=255)                                                                                                                                                           cagccaTCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATcc                                                                                        >  1:787783/1‑250 (MQ=255)                                                                                                                                                             gccaTCATTATACAAAAGATAAATGACGGCAGATATTTAGCGGAAAGGAGGAAAGGCAGAGCTATTCGTCACGTTAAATGGTCAACGCCCCCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCa                                                                                      <  1:527296/250‑1 (MQ=255)                                                                                                                                                                       ttaaaaagtttAATGCGGGCAATTATTATTCGGAAAGAAGAAAATGCATATCTTTTCTTAGTGTTAAATGTCAAACGCCCCCCCCCGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCAt                                                                             <  2:89575/240‑1 (MQ=255)                                                                                                                                                                         cAAAAGATTAATGACGGCAGATATTGATCGGAAAGGAGAAAAGGCAGAGTTTTTCGTCACTTAAAATGGTCAATGCCACCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCtt                                                                          <  1:309817/250‑1 (MQ=255)                                                                                                                                                                          aaCAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTg                                                                         >  2:745092/1‑250 (MQ=255)                                                                                                                                                                           aCAGATTAATGACGGCAGATATTTACCGGTAAGGAGAAAATGCAGAGTTTTTCGTCCCTTTCAATGGTCAATCCCCCCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGc                                                                        <  1:151401/250‑1 (MQ=255)                                                                                                                                                                            cAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGCTTCGGCAACCGGACGCGCCTCTCGTGCGACCGAGCGCGATCGCGCCCCCATTGACATTGACCCGCCGCTCATCCCACTCAAGCATCCTGc                                                                        >  2:4525/1‑249 (MQ=255)                                                                                                                                                                            cAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGc                                                                        >  1:556300/1‑249 (MQ=255)                                                                                                                                                                            cAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGc                                                                        >  2:398922/1‑249 (MQ=255)                                                                                                                                                                                   aTGACGGCAGATATTTTTCGGAAAGGAGGAAATGCAGAGTTATTCGCCCCTTTAAATGGTCAACCCCCCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGaa                                                                <  1:926027/250‑1 (MQ=255)                                                                                                                                                                                    tGACGACAGATATTTATGGGAAAGGAGGAAATGCAGATCTTTTCGTCAGTTTAAATGGTCAACGCCACCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGaaa                                                               <  1:913997/250‑1 (MQ=255)                                                                                                                                                                                     gACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAg                                                              >  1:393358/1‑250 (MQ=255)                                                                                                                                                                                           aGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCtg                                                        >  1:383241/1‑250 (MQ=255)                                                                                                                                                                                            aatatTTAACGGAAAGGAGAAAAGGAAGAGTTATTCGTCGCGTTAAAGGGACAAGCCCCCCCCCGGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCtgt                                                       <  1:853033/249‑1 (MQ=255)                                                                                                                                                                                                tttATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTgcag                                                   >  2:602979/1‑250 (MQ=255)                                                                                                                                                                                                ttaatgggaaaGGAGAAAAGGAAGAGTTTTTCGCCACGTTAAATGGCAAACCCCCCCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTgca                                                    <  1:566296/240‑1 (MQ=255)                                                                                                                                                                                                    gagatgaagagaaAATGCAGATTTATTCGACAATTTCAATGGACAACCCCCCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCaaa                                               <  2:949338/242‑1 (MQ=255)                                                                                                                                                                                                      ggTAAGGAGAAAATGCAGAGCTTTTCGTCACGTTCAAGGGTCAACCCCCCCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAAc                                              <  1:253862/249‑1 (MQ=255)                                                                                                                                                                                                        tAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATAAAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTAATGAACCAGAGAAACCAGGTTCCGGCAACCGGAACCGCCTATCGGGGGACCGAGTGGGATCGCGCAACCATGAACATTGACCCACGGCCCATCCCACTCACGCACCTTGCCAACCGAAAGCGCTTGTGCACCAAACGCt                                           >  2:419804/1‑250 (MQ=255)                                                                                                                                                                                                           gaagaaaagcaagagTTATTCGTCACTTTAAATGGCCAATCCCACCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCa                                        <  1:7154/239‑1 (MQ=255)                                                                                                                                                                                                            gaggaAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCAt                                       >  1:102914/1‑250 (MQ=255)                                                                                                                                                                                                                aaaGGCAGACCTTTTCGCCACGTTAAATGGTAAATCCCCCCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCAGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGc                                   <  1:343753/250‑1 (MQ=255)                                                                                                                                                                                                                 attgaagagCTATCCATCGCGTTAAAGGGTAAACCCCACCCCCCGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGcc                                  <  2:958120/245‑1 (MQ=255)                                                                                                                                                                                                                 aaTGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGcc                                  >  1:673630/1‑250 (MQ=255)                                                                                                                                                                                                                   tGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTCCATTAGCCTc                                >  2:786143/1‑250 (MQ=255)                                                                                                                                                                                                                     cAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGa                              >  1:889083/1‑250 (MQ=255)                                                                                                                                                                                                                     cAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGa                              >  1:819858/1‑250 (MQ=255)                                                                                                                                                                                                                      agagCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGa                              >  1:757084/1‑249 (MQ=255)                                                                                                                                                                                                                         gCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGAt                             >  1:757162/1‑247 (MQ=255)                                                                                                                                                                                                                           tATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGAt                             >  1:921215/1‑245 (MQ=255)                                                                                                                                                                                                                            tttCGCCCCGTTCAAGGGTCAACCCCCCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGAt                        <  1:145041/248‑1 (MQ=255)                                                                                                                                                                                                                                       tCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGcc             >  2:192975/1‑249 (MQ=255)                                                                                                                                                                                                                                         aaTGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAAc          <  1:179968/250‑1 (MQ=255)                                                                                                                                                                                                                                          gtGGTCACTGCCCCCCCCGGCCCTCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACt         <  1:351280/249‑1 (MQ=255)                                                                                                                                                                                                                                            ggTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGc       >  2:506555/1‑250 (MQ=255)                                                                                                                                                                                                                                                 agcccACCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGcc  <  1:515144/247‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   TAAAGGGATGATATGCCCATGGGCTAGGGTTTTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCC  >  NZ_CP009273/2977553‑2978042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑