Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,036,390	0	A	G	41.5%	71.5 / 15.0	54	N206D (AAT→GAT)	srkA	stress response kinase SrkA
Reads supporting (aligned to +/- strand):  ref base A (11/20);  new base G (22/0);  total (34/20)
Fisher's exact test for biased strand distribution p-value = 4.35e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATA‑TTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGC  >  NZ_CP009273/4036151‑4036631                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   ggCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATA‑TTCTCTg                                                                                                                                                                                                                                         <  1:84267/250‑1 (MQ=255)     ttATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTTCATGGGGACTGCCACGCCGGGGATA‑TTTTCTGgcgc                                                                                                                                                                                                                                     >  1:217137/1‑250 (MQ=255)       aTTTAGGGCGTATGCACCAGACGTGGCGCACACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATA‑TTCTCTGGcgcg                                                                                                                                                                                                                                    <  2:308766/249‑1 (MQ=255)             ggCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTTTGCGGCTACCAGGAGAATGCCACACCGGGGATA‑TTCTCTGGCGCGGTgggc                                                                                                                                                                                                                              >  1:279863/1‑247 (MQ=255)                gTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGAATTTACCGTTCTTCGGGTACATGGAGACTGCCCCGCCGGGAGTA‑TTTTTTGGCGCGATGGGCCCAt                                                                                                                                                                                                                          >  1:140712/1‑250 (MQ=255)                  aTGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGAATTCACCGTTCTTCGGCCACATGGGGACTGCCAAGCCGGGGATA‑TTCTCTTGCGCGATGGGCCCAttg                                                                                                                                                                                                                        >  1:275764/1‑248 (MQ=255)                                  gcAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACCCTGGCGGGCAGATTTCACCCGTCTGCGCCTACCTTGAGACTTCAACACCCGGCATATTTCTTTGG‑GCGCTGGTGCCATGTtttttgtgctcgag                                                                                                                                                                                                          >  2:727160/1‑240 (MQ=255)                                   cAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACGGCACACTGGCGGGGAGAATTTACCGTTTTGCGGCTAAAAGGAGACTGCCACGCCGGGGATA‑TTCTCTGGGGCGATGGGCCCATGGTTGTTGATCTGgttgat                                                                                                                                                                                                       >  1:153135/1‑250 (MQ=255)                                    aaaCAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTTCCACGCCGGGAATA‑TTCTCTGGCGCGATGGTCCCATGTTTTTTTGTCTGggtgatt                                                                                                                                                                                                      >  1:945446/1‑249 (MQ=255)                                    aaaCAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATA‑TTTTCTGGCGCGATGGGTCAATGTTTGTTTATCTGGgtgatg                                                                                                                                                                                                      >  1:281180/1‑250 (MQ=255)                                             tttATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTCACCGTTCTGCGGGTACATGGGGACTGCCACGCCGGGGATA‑TTTTCTTGGGCGAGGGGCCAATGTTTGTTGATCTGGGTGATGCATGTAATg                                                                                                                                                                                             >  1:843239/1‑250 (MQ=255)                                                 tCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCCCCGTTCTGCGGCGACATGGGGAATGCCACGCCGGGGAAA‑TTCTCCTGGGGGATGGTGCCATGTTTGTTGATCTGGATGTTGCACGTAATGGTc                                                                                                                                                                                          >  2:812269/1‑249 (MQ=255)                                                  ccATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATA‑TTTTCTGGCGCGATGGGCCAATGTTTGTTGTTCTGGGTGATGCACGGAATgggc                                                                                                                                                                                          >  1:469460/1‑246 (MQ=255)                                                         ccGACCTTCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATA‑TTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTc                                                                                                                                                                                 <  2:85748/250‑1 (MQ=255)                                                             ccATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTGGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATA‑TTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGa                                                                                                                                                                             <  2:389880/250‑1 (MQ=255)                                                                 cGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATA‑TTTTCTGGCGCGGTGGTCCCATGGTTGTTGTTCTGGGTGATGCACGTAATTGTCCAGCCGTTTCGGGTTtg                                                                                                                                                                         >  1:112790/1‑250 (MQ=255)                                                                   gTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATA‑TTTTCTGGCGCGGTGGTCCCATGTTTGTTGGTCTTGGTGATGCACGTAATGGTCCAGCCGTTTAGGGTTtgtg                                                                                                                                                                       >  1:545893/1‑250 (MQ=255)                                                                        aaCGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATA‑TTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGt                                                                                                                                                                  <  2:653496/250‑1 (MQ=255)                                                                             atatCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATA‑TTCTCTGGCGCGATGGGCCCATGTTTGTTGATCTGGATGATGCACGTAATGGGCCAGCCGTTCAGGATTTGTGGGTGTTGCt                                                                                                                                                              >  1:232829/1‑249 (MQ=255)                                                                                tctcATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGGGACCGCCACGCCGGGGATA‑TTTTCTTGGGCGATGGGCCAATGTTTGTTGATTTGGGTGATGCACGTAATGGTCCAGCCGTTTAGGATTTGTGGGTGTTGCTCAAt                                                                                                                                                          >  1:792615/1‑250 (MQ=255)                                                                                         gCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATA‑TTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATaaa                                                                                                                                                 <  2:928034/250‑1 (MQ=255)                                                                                           cACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATA‑TTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCCGGGTTTGTGGGTGTTGCTCAATGGCGATAAAGc                                                                                                                                               >  1:239529/1‑250 (MQ=255)                                                                                                         aaGGACGCAACACTGATCCCTGCCGGGTTAAAAGCGGCATTCCTGAAAGCGACAGATAGGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATA‑TTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATg                                                                                                                                  <  2:506028/248‑1 (MQ=255)                                                                                                             aCGCTACCCTGATCCCTTCCGGGTTGAAAGCGGCATCCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATA‑TTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACt                                                                                                                             <  2:616349/250‑1 (MQ=255)                                                                                                              cGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGGGACTGCCACGCCGGGGATA‑TTCTCTGGCGCGATGGGCCCATGTTTGTTGGTCTGGGTGATGCACGGAATGGTCCAGCCGTTCAGGATTTTTGGGTGTTGGTCAATGGCGGTAAAGCCGAGGAGCGGGTGCAACTg                                                                                                                            >  1:403942/1‑250 (MQ=255)                                                                                                                    acTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCCCCGGTCTGCGGCTACATGGGGACCGCCACGCCGGGGATA‑TTTTCTTGCGCGGGGGGTCCATGTTTTTTTGTCTTGGTGATGGACGGAATTGGCCCGCCGGTTAGGGTTTTTTGGTGTTGTTCCATGGCGGTAAAGCCGCGAAGCGGGTGCAAATGGgaaa                                                                                                                       >  2:374412/1‑248 (MQ=255)                                                                                                                     cTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATA‑TTTTCTGGCGCGATGGGCCAATGTTTGTTGATCTGGGTGATGCACGTAATGGTCCAGCCGTTCAGGGTTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACta                                                                                                                     >  1:137283/1‑250 (MQ=255)                                                                                                                       gATACCTGCCGGGTGAAAAGCGGCAGTCCTGAAAGCGACATATGAGCGGATTGCCGCCGTTGCAGCACTCTGGCGGGAAGATTTCACCTGTCTGCGGCTACATGGAGACTGCCACGTCGGGAATA‑TTCTCTGGCGCGATGGTCCAATGTGTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACtat                                                                                                                    <  2:633378/249‑1 (MQ=255)                                                                                                                        aTACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATA‑TTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACtatta                                                                                                                  <  1:824783/250‑1 (MQ=255)                                                                                                                                 gggTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATA‑TTCTCTGGCGCGATGGGCCCATGTTTTTTGTTCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGGTTTTTGGGTGTTGTTCCATGGGGATAAAACCGGGCAGCGGGTGCAAATTGAAACCATTATTTAAGCtt                                                                                                         >  2:257681/1‑250 (MQ=255)                                                                                                                                    ttGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATA‑TTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATg                                                                                                      <  2:764502/250‑1 (MQ=255)                                                                                                                                            ggCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGATACTGCCACGCCGGGAATA‑TTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAAttt                                                                                              <  1:956115/250‑1 (MQ=255)                                                                                                                                                   cTGAAAGCGACAGAGGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATA‑TTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGt                                                                                       <  2:267283/250‑1 (MQ=255)                                                                                                                                                    tGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTTACCGGTCTGCGGCCACAATGAGACTGCCCCCCCGGGGATA‑TTTTCTTGCGCGGTGGGCCAATGTTTGTTGTTCTGGATGATGCACGGAATGGTGCAGCCGCTCCGGGGTTGTGGGTTTTGTTCCATGGCGATAAAACCGGGCCGCGGGAGGAACTGGGAACAATTTTTGTAGCTTATGAAGAATTTTGagaggt                                                                                      >  2:38678/1‑248 (MQ=255)                                                                                                                                                     gAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGGGACTGCCCCGCCGGGGATA‑TTCTCTTGCGCGATGGTCCCATGTTTGTTGATCTGGGTGATGCACGTAATGGGCCAGCCGTTCAGGGGTTGTGGATGTTTGTCAATGGCGATAAAGCCGAGCAGCGGGAGCAACTGGGAACTATTATTTAAGCTTATGAAGAAATTAGGGAGGTc                                                                                     >  2:344496/1‑250 (MQ=255)                                                                                                                                                     gAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTTAACGGTTTGCGGCGACATGGAGACTGCCCCGCCGGGGATA‑TTTTCTGGGGGGGTGGGCCAATGTTTGTTGTTCTTGATGATGGACGTGATGGTCCCGCCGTTTAGGGTTTGTGGGTGTTGGTCAATGGCGATAAAGGCGAGCAGCGGGTGCAACTGGATAATATTATTTAAGCTTATGAAGAATTTAGAGAGTTc                                                                                     >  2:869067/1‑250 (MQ=255)                                                                                                                                                       aaGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATA‑TTCTCTGGCGCGATGGTCCCATGTTTGTTGATCTGGGTGATGCACGTAATGGTCCAGCCGTTCAGGGTTTTTGGGTGTTGCTCAATGGCGGTAAAGCCGAGCAGCGGGTGCAAATGGAAAATATTATTGAAGCTTATGAAGAAATTAACGAGTTTGa                                                                                   >  1:401248/1‑250 (MQ=255)                                                                                                                                                                aTGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATA‑TTCTCTGGCGCGATGGTCCCATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGGTGTTGCTCAATGGCGATAAAGCCGAGCAGCGGGTGGAACTGGAAACTATTTTTTAAGCTTATGAAGAATTTAACGAGTTCGACACCGCtt                                                                           >  1:123606/1‑248 (MQ=255)                                                                                                                                                                aTGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATA‑TTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGGTTTGTGGATGTTGCTCAATGGCGATAAAACCGAGCAGCGGGTGCAACTGGAAAATATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTg                                                                           >  1:343323/1‑249 (MQ=255)                                                                                                                                                                aTGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATA‑TTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGa                                                                          <  1:836528/250‑1 (MQ=255)                                                                                                                                                                    gCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATA‑TTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATc                                                                      <  2:605144/250‑1 (MQ=255)                                                                                                                                                                       gATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATA‑TTTTCTGGCGCGATGGGCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGGTGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGGTCGACACCGCTGAAATCGGa                                                                   >  1:337496/1‑250 (MQ=255)                                                                                                                                                                       gATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATA‑TTCTCTGGCGCGATGGGCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGGTGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGGAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGa                                                                   >  1:266465/1‑250 (MQ=255)                                                                                                                                                                         ttGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATA‑TTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACt                                                                 <  1:265975/250‑1 (MQ=255)                                                                                                                                                                               ccgTTACAGCACACTGGCGGGAAGATTTCCCCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATA‑TTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGa                                                           <  2:383134/250‑1 (MQ=255)                                                                                                                                                                               ccgTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATA‑TTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGa                                                           <  1:179967/250‑1 (MQ=255)                                                                                                                                                                                          cacTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATA‑TTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTAcgcg                                                <  2:370960/250‑1 (MQ=255)                                                                                                                                                                                                       aTTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATA‑TTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGt                                   >  2:914879/1‑250 (MQ=255)                                                                                                                                                                                                                     cGGCTACATGGAGACTGCCACGCCGGGAATA‑TTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGcc                      <  2:217137/249‑1 (MQ=255)                                                                                                                                                                                                                     cGGCTACATGGAGACTGCCACGCCGGGAATA‑TTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCt                     >  2:846214/1‑250 (MQ=255)                                                                                                                                                                                                                      ggCTACATGGAGACTGCCACGCCGGGAATA‑TTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTg                    >  2:827802/1‑250 (MQ=255)                                                                                                                                                                                                                           cATGGAGACTGCCACGCCGGGAATA‑TTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGTGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTac               >  2:870718/1‑249 (MQ=255)                                                                                                                                                                                                                                      ccACGCGGGGATTA‑TTCTCTGGCGCGATGGTTCCATGTTTGTTGATCTGGATGATGCACGTAATGGACCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGCTGCAACTGGAAACTATTATTGAAGCTTATGAAGTATATAGCGAGTTCGACAAAGATGAAATCGGACGGATGGAACCTTTAAGCGCCATGCGGTTGGATTAGTAGCTTGACTGGCTAATgaggcgcggg    >  2:377070/1‑246 (MQ=255)                                                                                                                                                                                                                                        aCGCCGGGAATA‑TTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGc  >  1:96409/1‑250 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   GGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATA‑TTCTCTGGCGCGATGGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTATTATCTTGCCTGGCTAATGCGGCGTTGGGC  >  NZ_CP009273/4036151‑4036631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑