Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,819,288	0	C	T	37.5%	65.9 / 31.2	50	intergenic (‑94/+109)	ves/spy	environmental stress‑induced protein Ves/ATP‑independent periplasmic protein‑refolding chaperone Spy
Reads supporting (aligned to +/- strand):  ref base C (28/2);  new base T (0/18);  total (28/22)
Fisher's exact test for biased strand distribution p-value = 2.60e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCA‑CCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCAT  >  NZ_CP009273/1819041‑1819537                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                             aCAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCA‑CCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGTTTT‑ACGTTAGTGGTGATCAGGCtt                                                                                                                                                                                                                                                            >  2:491765/1‑250 (MQ=255)    aaGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCA‑CCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGTTCAGGCttttt                                                                                                                                                                                                                                                         >  1:612852/1‑250 (MQ=255)          aCTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCA‑CCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑Ca‑a                                                                                                                                                                                                                                                 >  2:140278/1‑250 (MQ=255)                  ttCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCA‑CCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCACCTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGttt                                                                                                                                                                                                                                          >  2:602317/1‑248 (MQ=255)                    cgccgcGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCA‑CCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTgag                                                                                                                                                                                                                                       >  1:168863/1‑249 (MQ=255)                        gcgATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCA‑CCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTgagaagag                                                                                                                                                                                                                                  <  1:600245/250‑1 (MQ=255)                                    aTGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCA‑CCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAAccg                                                                                                                                                                                                                      >  1:550766/1‑250 (MQ=255)                                     tGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCA‑CCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAAccgc                                                                                                                                                                                                                     >  1:622056/1‑250 (MQ=255)                                                       aaTCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCA‑CCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCAt                                                                                                                                                                                                   >  2:433399/1‑250 (MQ=255)                                                            ctgttgcgcGGGGGGGACCTGCCAATTTCCCCCGTTTCACCCGGCAGTTTCCCCCCACAGATCCACCGAATTTTTACCAATAAAAAAT‑TTTCCCATTCC‑CGCCCCCGGCCCTTTTTTTTTT‑TTTTTAT‑GTTTCAGG‑AACCGGGGG‑TGGTTTTTCGGAACCGGGGTTGATTT‑ACGTTGGTGGTAATCAGGTTTTTTTTC‑AA‑CC‑TGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACt                                                                                                                                                                                               <  2:352882/242‑1 (MQ=255)                                                             gTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCA‑CCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                                                                                                                              >  2:33632/1‑249 (MQ=255)                                                                     ggTGGGAACGTGCAAATTTCGCGCGTTTCA‑CCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGt                                                                                                                                                                                     >  2:624403/1‑250 (MQ=255)                                                                         ggAACGTGAAAATTCCCCGCGTTCCC‑CCGGCACCATTTCACCAAAATTTCCCCTATA‑TTTACATAATTTAAAGTTCTTCCATCCT‑CCCCCCCGGCCTTTTTTTTTCA‑TTGTTTG‑GGTTCAGGT‑ACCGGGGG‑TTG‑TTTACGGGCACCGGGGTTATTTAAGGTTGGTGGT‑AACCGGGTTTTTTTA‑CC‑ACGTTTTGGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGAc                                                                                                                                                                                 <  2:454018/250‑1 (MQ=255)                                                                               tGCAAATTTCGCGCGTTTCA‑CCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGAcc                                                                                                                                                                           >  1:311670/1‑250 (MQ=255)                                                                                gCAAATTTCGCGCGTTTCA‑CCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcg                                                                                                                                                                          >  1:610948/1‑250 (MQ=255)                                                                                        cgcgctTTTCA‑CCGGAACCTTTCCCCCAAATTTCCACCGACATTTTACGCATAACAAAT‑TATCCCATCCT‑CCCTCCCGGCCGTTTCTTTTCT‑TTTTTAT‑CGTTAAGGTACCGGTGGT‑TGT‑TTATCGGCAACTGGGGTAATTT‑ACGTTAGTGGTGATCAGTTTTTTTTTA‑CA‑AC‑TTTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCt                                                                                                                                                                   <  1:649677/249‑1 (MQ=255)                                                                                                    acGGCAGCATTCCGCCCAAGATCCACCGACATTTTCCGCTTATCAAAT‑TTTTCCATACT‑CGCTCCCGGCTCTTTTTTTTCA‑TTTTTAT‑CTTTCAGGTAACCGGGGA‑TGG‑TTATCGACAACGCGGGTTATTA‑AGTTTAGTGGGGATCGGGTTTTTTTAA‑CA‑AC‑TTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTgg                                                                                                                                                       <  2:299399/249‑1 (MQ=255)                                                                                                          gcatcgcGCCACACATTCACCTACTTTTTTCTGATATCAAAG‑TTTTCTCTACG‑C‑CGCCCGGGCTGTTTTTTTGATTTGTGTT‑CTTGCAGGTAGCCGGGTG‑GGG‑TTTCCGGCAACTCGGTTTATTT‑ACGTAAGTGGTGATCGGTTTTTTTTTA‑AA‑AC‑TTTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGaaa                                                                                                                                                 <  2:103207/244‑1 (MQ=255)                                                                                                            aTTTCGCCACAGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGACTTTAGTTTTTTGGCTGAAAg                                                                                                                                                >  2:429710/1‑249 (MQ=255)                                                                                                             ttcccccACAGATCCCCCGACATTTTACG‑ATAATAAAA‑TTTTCCCTTCTCCGCCCCCGGCCC‑TTTTTTTTT‑TTGTTTTGGGTTAAGGTACCGGGGGA‑GGT‑TTATCGGAACCGGGGGTGATTG‑AGGTTGGTGGTGTTCGGGTTTTTTTAA‑CA‑CT‑TTTTGGAAAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGa                                                                                                                                               <  2:681025/244‑1 (MQ=255)                                                                                                               tccccACAGATTCCCCACAATTTTCCGAAAATCAAAG‑TATTCCATCCT‑CGCTCCCGCCCCGTTCTTTTCA‑TTTTTAT‑CGTTCAGTTAACGGGGGA‑TGT‑TTACCGGCACCTGGGGTGATTT‑ACGTTAGTGTTAATCA‑GCTTTTTTTACCA‑AC‑TTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGatt                                                                                                                                             <  1:154966/246‑1 (MQ=255)                                                                                                                      aGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcag                                                                                                                                       >  2:141729/1‑248 (MQ=255)                                                                                                                           ccccgcctttttACGCATATAAAGG‑TTTCCCATCCT‑CGCCCCCGCCCCTTTTTTTTCA‑TTTTTTT‑GTTCCAGGAACCGGGGGA‑TGT‑TTACCGGAAACTGGGTTTTTTT‑ACGTTGGTGGTGTTCAGGTTTTTTTTA‑CA‑AT‑TGTTTGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTg                                                                                                                                 <  1:443242/241‑1 (MQ=255)                                                                                                                           cACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTATTAAGTTTTTTGGCTGAAAGATTATTCCGCAGTTGc                                                                                                                                >  2:54907/1‑250 (MQ=255)                                                                                                                                   ttttACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTTCAGGCAttt                                                                                                                        >  1:6745/1‑250 (MQ=255)                                                                                                                                    tttACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTTAGAAGTTGCAGGCAtttt                                                                                                                       >  1:378412/1‑250 (MQ=255)                                                                                                                                                        tCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGCTTAAGTTTTTTGGGTGAAAGATTATTTAACAGTTTGAGGGATTTTTACTTTTTCCGCTGGGCgg                                                                                                    >  2:373226/1‑249 (MQ=255)                                                                                                                                                                 ctcTCCCGGCCTGTTCTTTTCA‑TTGTTAT‑CGTTGAGGTAACCGTGGA‑TGT‑TTATCGGCACCTGGGGTGATTT‑ACGTTAGTGGTGATCAGTTTTTTTTTA‑CA‑TT‑TGTTGGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAg                                                                                            <  2:550766/248‑1 (MQ=255)                                                                                                                                                                   cccccGGCCCTTTTTTTTAT‑TTTTTTT‑CGTTCAGTAACCCGGGGA‑TGT‑TATTCGGCAACTGGGGTTATTT‑ACTTAGGTGGTGATCAGGTTTTTTTTA‑CA‑CT‑TGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGAc                                                                                          <  1:525682/248‑1 (MQ=255)                                                                                                                                                                   cTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGGATTTTACCTTTTGCCGGTGGACGTTCTGTCAg                                                                                            >  2:165204/1‑248 (MQ=255)                                                                                                                                                                    ccccGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGG‑TGG‑TTATCGGCAACTGGGGTGATTT‑ACGTTAGTGGTGATCAGTTTTTTTTTA‑CA‑CC‑TGTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACg                                                                                         <  1:433399/249‑1 (MQ=255)                                                                                                                                                                      ccGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTTAGCAGTTGCAGGCATTTTTACTTTTTCCCCCGGCCGGTTTGTCAGTCGCt                                                                                       >  2:454125/1‑250 (MQ=255)                                                                                                                                                                        ggccttttCTTTTTA‑TTGTTTT‑CTTTCAGGTACCGGGGGT‑TGG‑TTATCGGCAACTGGGGTGATTT‑ACGTGAGGGGTGATCAGTTTTTTTTTA‑CA‑AC‑TGTTTGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACCGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTtc                                                                                     <  2:168863/244‑1 (MQ=255)                                                                                                                                                                            cGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTATGCTTTTTGGCTGAAAGAGTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTTGTCGTTCTGCTAGACGCTTCTCa                                                                                  >  2:387784/1‑249 (MQ=255)                                                                                                                                                                                   ttCA‑TTTTTAT‑CGTTCAGGTACCCGTGGT‑TGG‑TTATCGGCACCTGGGGTGTTTT‑ACGTTGGTGGTGATCAGGTTTTTTTTA‑CA‑AC‑TGTTTAAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAg                                                                           <  1:140278/249‑1 (MQ=255)                                                                                                                                                                                    ttatttGTTTT‑GGTTCAGATCACTGTGGA‑GGT‑TTTTCGGAACCTGGGGTATTTT‑ACGTTAGTGGTAATCAGGTTTTTTTTA‑CA‑AC‑TGTTTGAGAAAAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAAtt                                                                             <  1:25735/243‑1 (MQ=255)                                                                                                                                                                                            tAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTTAAAATTAGCATTAAAtt                                                                  >  1:216946/1‑250 (MQ=255)                                                                                                                                                                                             taccgtttgggTAACCGGTGA‑TGT‑TTTTCGGAAACTCGTTTGTTTA‑ACGTGAGTGGTTATCAGTTTTTTTTAA‑CA‑TC‑TTTTAGAAAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAAt                                                                   <  1:73669/240‑1 (MQ=255)                                                                                                                                                                                             gttggTTCAGTTAACGGTGGA‑TGT‑TTATCGGCAACTGGGTTGATTT‑ACGTTAGTGTTAACAAGGTTTTTTTTA‑CA‑AT‑TGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAAt                                                                   <  1:559495/245‑1 (MQ=255)                                                                                                                                                                                               atcgttatgataCCGTGGGTTGG‑TTATCGGCAGTTCGGTTGATTT‑TTGTTGGGGTTGATCAGG‑TTTTTTTA‑CACAT‑TGTTAGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAAtt                                                                  <  2:610948/238‑1 (MQ=255)                                                                                                                                                                                                cGTTCAGGTACCGGTGGA‑TGT‑TTACCGGAACCTGGGGTGTTTT‑AGGTTGGTGGGAATGAGTTTTTTTTTA‑CA‑AT‑TTTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGc                                                                <  1:19510/249‑1 (MQ=255)                                                                                                                                                                                                  ttCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGACGGCATTTTACCTTTTGCCGCTGGACGTTTTTTCAGGCGCTTCTCAAAATTAACCTTaaaattgttt                                                             >  2:438277/1‑243 (MQ=255)                                                                                                                                                                                                           ccGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTcc                                                    >  1:140417/1‑250 (MQ=255)                                                                                                                                                                                                                       ttATCGGAAACTGGGGTGATTT‑GCGTTAGTGGTGATCAGGTTTTTTTTA‑CA‑AC‑TGTTTAAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGa                                          <  1:9296/250‑1 (MQ=255)                                                                                                                                                                                                                              cAACGCGGTTGATTT‑AGTTTAGTGGTGAGCCTGTTTTTTTTA‑CA‑CC‑TGTAGGAAAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTa                                   <  2:528262/250‑1 (MQ=255)                                                                                                                                                                                                                               aaCTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTTAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGGCGTTCTGTCAGACGCTTCTCAAAATTAGCATTTAATTGCTTTTTCTTTTTCCGCGTCAGGGtggtttg                                   >  2:594656/1‑244 (MQ=255)                                                                                                                                                                                                                                  gggggTGATTT‑ACTTTAGTGGTGATCAGTTTTTTTTTA‑CA‑AC‑TGTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAAtt                               <  1:33632/248‑1 (MQ=255)                                                                                                                                                                                                                                    gggTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTTTGTTCCGGCGTCAGGATGTTGTAAAtttt                             >  1:351776/1‑250 (MQ=255)                                                                                                                                                                                                                                                 ttAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTc                 >  1:520494/1‑250 (MQ=255)                                                                                                                                                                                                                                                       gtgATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTTCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAATGTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGTCAGAAGCTTATCTAAATTTGCACTAAATTTCTTTTTTTGTTCCGGCGTCCGGGTGTTGTAAATTTTTTTCTTGGTTTTCAtgtg           >  2:354621/1‑250 (MQ=255)                                                                                                                                                                                                                                                                cTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGGTTCCATGTGCGCCAGCAt  >  1:679331/1‑250 (MQ=255)                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                             ACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCA‑CCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCAT  >  NZ_CP009273/1819041‑1819537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑