Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,819,3130GA41.5% 68.1 / 29.7 53intergenic (‑119/+84)ves/spyenvironmental stress‑induced protein Ves/ATP‑independent periplasmic protein‑refolding chaperone Spy
Reads supporting (aligned to +/- strand):  ref base G (29/2);  new base A (0/22);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 5.97e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCA‑CCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCA  >  NZ_CP009273/1819064‑1819560
                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       
gcgATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCA‑CCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTgagaagag                                                                                                                                                                                                                                                         <  1:600245/250‑1 (MQ=255)
            aTGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCA‑CCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAAccg                                                                                                                                                                                                                                             >  1:550766/1‑250 (MQ=255)
             tGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCA‑CCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAAccgc                                                                                                                                                                                                                                            >  1:622056/1‑250 (MQ=255)
                               aaTCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCA‑CCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCAt                                                                                                                                                                                                                          >  2:433399/1‑250 (MQ=255)
                                    ctgttgcgcGGGGGGGACCTGCCAATTTCCCCCGTTTCACCCGGCAGTTTCCCCCCACAGATCCACCGAATTTTTACCAATAAAAAAT‑TTTCCCATTCC‑CGCCCCCGGCCCTTTTTTTTTT‑TTTTTAT‑GTTTCAGG‑AACCGGGGG‑TGGTTTTTCGGAACCGGGGTTGATTT‑ACGTTGGTGGTAATCAGGTTTTTTTTC‑AA‑CC‑TGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACt                                                                                                                                                                                                                      <  2:352882/242‑1 (MQ=255)
                                     gTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCA‑CCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                                                                                                                                                     >  2:33632/1‑249 (MQ=255)
                                             ggTGGGAACGTGCAAATTTCGCGCGTTTCA‑CCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGt                                                                                                                                                                                                            >  2:624403/1‑250 (MQ=255)
                                                 ggAACGTGAAAATTCCCCGCGTTCCC‑CCGGCACCATTTCACCAAAATTTCCCCTATA‑TTTACATAATTTAAAGTTCTTCCATCCT‑CCCCCCCGGCCTTTTTTTTTCA‑TTGTTTG‑GGTTCAGGT‑ACCGGGGG‑TTG‑TTTACGGGCACCGGGGTTATTTAAGGTTGGTGGT‑AACCGGGTTTTTTTA‑CC‑ACGTTTTGGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGAc                                                                                                                                                                                                        <  2:454018/250‑1 (MQ=255)
                                                       tGCAAATTTCGCGCGTTTCA‑CCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGAcc                                                                                                                                                                                                  >  1:311670/1‑250 (MQ=255)
                                                        gCAAATTTCGCGCGTTTCA‑CCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcg                                                                                                                                                                                                 >  1:610948/1‑250 (MQ=255)
                                                                cgcgctTTTCA‑CCGGAACCTTTCCCCCAAATTTCCACCGACATTTTACGCATAACAAAT‑TATCCCATCCT‑CCCTCCCGGCCGTTTCTTTTCT‑TTTTTAT‑CGTTAAGGTACCGGTGGT‑TGT‑TTATCGGCAACTGGGGTAATTT‑ACGTTAGTGGTGATCAGTTTTTTTTTA‑CA‑AC‑TTTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCt                                                                                                                                                                                          <  1:649677/249‑1 (MQ=255)
                                                                            acGGCAGCATTCCGCCCAAGATCCACCGACATTTTCCGCTTATCAAAT‑TTTTCCATACT‑CGCTCCCGGCTCTTTTTTTTCA‑TTTTTAT‑CTTTCAGGTAACCGGGGA‑TGG‑TTATCGACAACGCGGGTTATTA‑AGTTTAGTGGGGATCGGGTTTTTTTAA‑CA‑AC‑TTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTgg                                                                                                                                                                              <  2:299399/249‑1 (MQ=255)
                                                                                  gcatcgcGCCACACATTCACCTACTTTTTTCTGATATCAAAG‑TTTTCTCTACG‑C‑CGCCCGGGCTGTTTTTTTGATTTGTGTT‑CTTGCAGGTAGCCGGGTG‑GGG‑TTTCCGGCAACTCGGTTTATTT‑ACGTAAGTGGTGATCGGTTTTTTTTTA‑AA‑AC‑TTTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGaaa                                                                                                                                                                        <  2:103207/244‑1 (MQ=255)
                                                                                    aTTTCGCCACAGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGACTTTAGTTTTTTGGCTGAAAg                                                                                                                                                                       >  2:429710/1‑249 (MQ=255)
                                                                                     ttcccccACAGATCCCCCGACATTTTACG‑ATAATAAAA‑TTTTCCCTTCTCCGCCCCCGGCCC‑TTTTTTTTT‑TTGTTTTGGGTTAAGGTACCGGGGGA‑GGT‑TTATCGGAACCGGGGGTGATTG‑AGGTTGGTGGTGTTCGGGTTTTTTTAA‑CA‑CT‑TTTTGGAAAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGa                                                                                                                                                                      <  2:681025/244‑1 (MQ=255)
                                                                                       tccccACAGATTCCCCACAATTTTCCGAAAATCAAAG‑TATTCCATCCT‑CGCTCCCGCCCCGTTCTTTTCA‑TTTTTAT‑CGTTCAGTTAACGGGGGA‑TGT‑TTACCGGCACCTGGGGTGATTT‑ACGTTAGTGTTAATCA‑GCTTTTTTTACCA‑AC‑TTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGatt                                                                                                                                                                    <  1:154966/246‑1 (MQ=255)
                                                                                              aGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcag                                                                                                                                                              >  2:141729/1‑248 (MQ=255)
                                                                                                   ccccgcctttttACGCATATAAAGG‑TTTCCCATCCT‑CGCCCCCGCCCCTTTTTTTTCA‑TTTTTTT‑GTTCCAGGAACCGGGGGA‑TGT‑TTACCGGAAACTGGGTTTTTTT‑ACGTTGGTGGTGTTCAGGTTTTTTTTA‑CA‑AT‑TGTTTGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTg                                                                                                                                                        <  1:443242/241‑1 (MQ=255)
                                                                                                   cACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTATTAAGTTTTTTGGCTGAAAGATTATTCCGCAGTTGc                                                                                                                                                       >  2:54907/1‑250 (MQ=255)
                                                                                                           ttttACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTTCAGGCAttt                                                                                                                                               >  1:6745/1‑250 (MQ=255)
                                                                                                            tttACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTTAGAAGTTGCAGGCAtttt                                                                                                                                              >  1:378412/1‑250 (MQ=255)
                                                                                                                                tCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGCTTAAGTTTTTTGGGTGAAAGATTATTTAACAGTTTGAGGGATTTTTACTTTTTCCGCTGGGCgg                                                                                                                           >  2:373226/1‑249 (MQ=255)
                                                                                                                                         ctcTCCCGGCCTGTTCTTTTCA‑TTGTTAT‑CGTTGAGGTAACCGTGGA‑TGT‑TTATCGGCACCTGGGGTGATTT‑ACGTTAGTGGTGATCAGTTTTTTTTTA‑CA‑TT‑TGTTGGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAg                                                                                                                   <  2:550766/248‑1 (MQ=255)
                                                                                                                                           cccccGGCCCTTTTTTTTAT‑TTTTTTT‑CGTTCAGTAACCCGGGGA‑TGT‑TATTCGGCAACTGGGGTTATTT‑ACTTAGGTGGTGATCAGGTTTTTTTTA‑CA‑CT‑TGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGAc                                                                                                                 <  1:525682/248‑1 (MQ=255)
                                                                                                                                           cTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGGATTTTACCTTTTGCCGGTGGACGTTCTGTCAg                                                                                                                   >  2:165204/1‑248 (MQ=255)
                                                                                                                                            ccccGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGG‑TGG‑TTATCGGCAACTGGGGTGATTT‑ACGTTAGTGGTGATCAGTTTTTTTTTA‑CA‑CC‑TGTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACg                                                                                                                <  1:433399/249‑1 (MQ=255)
                                                                                                                                              ccGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTTAGCAGTTGCAGGCATTTTTACTTTTTCCCCCGGCCGGTTTGTCAGTCGCt                                                                                                              >  2:454125/1‑250 (MQ=255)
                                                                                                                                                ggccttttCTTTTTA‑TTGTTTT‑CTTTCAGGTACCGGGGGT‑TGG‑TTATCGGCAACTGGGGTGATTT‑ACGTGAGGGGTGATCAGTTTTTTTTTA‑CA‑AC‑TGTTTGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACCGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTtc                                                                                                            <  2:168863/244‑1 (MQ=255)
                                                                                                                                                    cGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTATGCTTTTTGGCTGAAAGAGTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTTGTCGTTCTGCTAGACGCTTCTCa                                                                                                         >  2:387784/1‑249 (MQ=255)
                                                                                                                                                           ttCA‑TTTTTAT‑CGTTCAGGTACCCGTGGT‑TGG‑TTATCGGCACCTGGGGTGTTTT‑ACGTTGGTGGTGATCAGGTTTTTTTTA‑CA‑AC‑TGTTTAAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAg                                                                                                  <  1:140278/249‑1 (MQ=255)
                                                                                                                                                            ttatttGTTTT‑GGTTCAGATCACTGTGGA‑GGT‑TTTTCGGAACCTGGGGTATTTT‑ACGTTAGTGGTAATCAGGTTTTTTTTA‑CA‑AC‑TGTTTGAGAAAAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAAtt                                                                                                    <  1:25735/243‑1 (MQ=255)
                                                                                                                                                                    tAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTTAAAATTAGCATTAAAtt                                                                                         >  1:216946/1‑250 (MQ=255)
                                                                                                                                                                     taccgtttgggTAACCGGTGA‑TGT‑TTTTCGGAAACTCGTTTGTTTA‑ACGTGAGTGGTTATCAGTTTTTTTTAA‑CA‑TC‑TTTTAGAAAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAAt                                                                                          <  1:73669/240‑1 (MQ=255)
                                                                                                                                                                     gttggTTCAGTTAACGGTGGA‑TGT‑TTATCGGCAACTGGGTTGATTT‑ACGTTAGTGTTAACAAGGTTTTTTTTA‑CA‑AT‑TGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAAt                                                                                          <  1:559495/245‑1 (MQ=255)
                                                                                                                                                                       atcgttatgataCCGTGGGTTGG‑TTATCGGCAGTTCGGTTGATTT‑TTGTTGGGGTTGATCAGG‑TTTTTTTA‑CACAT‑TGTTAGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAAtt                                                                                         <  2:610948/238‑1 (MQ=255)
                                                                                                                                                                        cGTTCAGGTACCGGTGGA‑TGT‑TTACCGGAACCTGGGGTGTTTT‑AGGTTGGTGGGAATGAGTTTTTTTTTA‑CA‑AT‑TTTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGc                                                                                       <  1:19510/249‑1 (MQ=255)
                                                                                                                                                                          ttCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGACGGCATTTTACCTTTTGCCGCTGGACGTTTTTTCAGGCGCTTCTCAAAATTAACCTTaaaattgttt                                                                                    >  2:438277/1‑243 (MQ=255)
                                                                                                                                                                                   ccGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTcc                                                                           >  1:140417/1‑250 (MQ=255)
                                                                                                                                                                                               ttATCGGAAACTGGGGTGATTT‑GCGTTAGTGGTGATCAGGTTTTTTTTA‑CA‑AC‑TGTTTAAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGa                                                                 <  1:9296/250‑1 (MQ=255)
                                                                                                                                                                                                      cAACGCGGTTGATTT‑AGTTTAGTGGTGAGCCTGTTTTTTTTA‑CA‑CC‑TGTAGGAAAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTa                                                          <  2:528262/250‑1 (MQ=255)
                                                                                                                                                                                                       aaCTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTTAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGGCGTTCTGTCAGACGCTTCTCAAAATTAGCATTTAATTGCTTTTTCTTTTTCCGCGTCAGGGtggtttg                                                          >  2:594656/1‑244 (MQ=255)
                                                                                                                                                                                                          gggggTGATTT‑ACTTTAGTGGTGATCAGTTTTTTTTTA‑CA‑AC‑TGTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAAtt                                                      <  1:33632/248‑1 (MQ=255)
                                                                                                                                                                                                            gggTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTTTGTTCCGGCGTCAGGATGTTGTAAAtttt                                                    >  1:351776/1‑250 (MQ=255)
                                                                                                                                                                                                                         ttAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTc                                        >  1:520494/1‑250 (MQ=255)
                                                                                                                                                                                                                               gtgATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTTCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAATGTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGTCAGAAGCTTATCTAAATTTGCACTAAATTTCTTTTTTTGTTCCGGCGTCCGGGTGTTGTAAATTTTTTTCTTGGTTTTCAtgtg                                  >  2:354621/1‑250 (MQ=255)
                                                                                                                                                                                                                                        ttttttttA‑AA‑TC‑TGTTTGAAAAGAAAAAATAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCAt                         <  2:612852/249‑1 (MQ=255)
                                                                                                                                                                                                                                        cTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGGTTCCATGTGCGCCAGCAt                         >  1:679331/1‑250 (MQ=255)
                                                                                                                                                                                                                                           tttttA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGGTGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCCTGtt                      >  1:5228/1‑250 (MQ=255)
                                                                                                                                                                                                                                             tttA‑CA‑CC‑TTTTAGAAAAAAAAAAAAAACACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTa                     <  2:75580/249‑1 (MQ=255)
                                                                                                                                                                                                                                                a‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCtt                 >  1:638617/1‑250 (MQ=255)
                                                                                                                                                                                                                                                     aC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTgc              >  2:9118/1‑250 (MQ=255)
                                                                                                                                                                                                                                                          tttGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTTCATGTGCGCCAGCATGTTAGCTTTGCGCTg          >  1:341142/1‑250 (MQ=255)
                                                                                                                                                                                                                                                          tttGAGAAGAAAAAAGAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTg          <  1:13451/250‑1 (MQ=255)
                                                                                                                                                                                                                                                            tGAGAAGAGAAAAGCAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGATTTTTGGCTGGAAGATTATTCCGCACTTTTAGGCATTTTTCCTTTTGTCCCTGGGCGTTCTGTTAGACGCTTCTCCAAATTAGCATTCAATTGCTTTTTTTGTTCCGGGGGCCGGATGTTGTTAATTTTGTTTTGGGGTTTCATGTGTGCCCGCATGTTAGCTTTGCGGTGtt        >  2:56613/1‑250 (MQ=255)
                                                                                                                                                                                                                                                                  gagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGAAGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTTGGTTTCCATGTGTGCCAGCATGGTAGCTTTTCGCTGttttttcc  >  2:604925/1‑247 (MQ=255)
                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       
GCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCA‑CCGGCAGCATTTCGCCACAGATTCACCGACATTTTACGCATATCAAAG‑TATTCCATACT‑CGCTCCCGGCCCGTTCTTTTCA‑TTGTTAT‑CGTTCAGGTAACCGTGGA‑TGG‑TTATCGGCAACTCGGGTGATTT‑ACGTTAGTGGTGATCAGGCTTTTTTTA‑CA‑AC‑TGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGTCAGGATGTTGTAAATTTTGTTCTGGGTTTCCATGTGCGCCAGCATGTTAGCTTTGCGCTGTTCTTCCA  >  NZ_CP009273/1819064‑1819560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑