Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,419,8010TG36.7% 118.2 / 11.6 60L41V (TTG→GTG) cycAD‑serine/D‑alanine/glycine transporter
Reads supporting (aligned to +/- strand):  ref base T (4/34);  new base G (22/0);  total (26/34)
Fisher's exact test for biased strand distribution p-value = 1.06e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AATCTTAGTATTCATCCCGCGTATTGTTACCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGG  >  NZ_CP009273/4419554‑4420050
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                         
aaTCTTAGTATTCATCCCGCGTATTGTTACCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACGGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgtt                                                                                                                                                                                                                                                          <  1:656761/249‑1 (MQ=255)
      aGTTTTTATCCCGCGTATTGTTACCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGCCTGACTAAAGGCCGTAGAGCCTGAACACCACAGACAGGTACAGGAAGAAAAAAACATGGTAGAGCAGGTAAAAGTCGTTGCCGATGAGCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCACACAAACCGTCATATTCAGCTTAGTGCCATTGGCGGTGCCATTGGTACGGGgttgtt                                                                                                                                                                                                                                                       <  2:518672/246‑1 (MQ=255)
      aGTATTCATCCCGCGTATTGTTACCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTAt                                                                                                                                                                                                                                                    <  2:456726/249‑1 (MQ=255)
                       ttGTTACCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGGGCCCTTTGTCCGGGGGTGTTTTTGGGGTCTGGCAAAAAGAt                                                                                                                                                                                                                                  >  1:393387/1‑250 (MQ=255)
                            aCCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAGAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTTGGACGGGGGTGTTTTTGGGGGCTGGGAAAACAATTAGcc                                                                                                                                                                                                                             >  1:353837/1‑250 (MQ=255)
                                 atatCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCACAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGcc                                                                                                                                                                                                                        <  1:639586/250‑1 (MQ=255)
                                      gATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGGACGGGGGTGGTTATGGGGGTTGGGAAAACGATTAGCCTTGCCggggc                                                                                                                                                                                                                   >  1:6377/1‑248 (MQ=255)
                                      gATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGGTTTTTGGGTCTGGCAAAAAGATTAGTCTTTCCggggc                                                                                                                                                                                                                   >  1:615121/1‑248 (MQ=255)
                                       aTGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGCCAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCg                                                                                                                                                                                                                  <  2:552895/250‑1 (MQ=255)
                                            tCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGGACGGGGGTGTTTTTGGGGTCTGGCAAAACGATTAGCCTTGCAGGGGCGTCggt                                                                                                                                                                                                             >  1:650816/1‑248 (MQ=255)
                                              ccGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAATACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCa                                                                                                                                                                                                           <  1:160337/250‑1 (MQ=255)
                                               cGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACCGGGGTGTTTATGGGGTCTGGCAAAACGATTAGGCTTGCCGGGCCGTCGATCAt                                                                                                                                                                                                          >  1:610986/1‑250 (MQ=255)
                                                         tcgtcgTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGGCATTGGGACGGGGGTGTTTTTTGGGTGTTGCAAAAAGAATAGCCCTTCCGGGGCGGCGGTCCttttttttttt                                                                                                                                                                                                >  2:646525/1‑243 (MQ=255)
                                                               tATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATc                                                                                                                                                                                          <  1:610695/250‑1 (MQ=255)
                                                                     aGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGt                                                                                                                                                                                    <  2:427829/250‑1 (MQ=255)
                                                                       actgactAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGttt                                                                                                                                                                                  <  1:188482/250‑1 (MQ=255)
                                                                               aaGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGctct                                                                                                                                                                          <  1:589494/250‑1 (MQ=255)
                                                                                 ggCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGGCATTTGGACGGGGGTGTTTTTGGGGGCTGGCAAAACGATTAGCCTTGCCGGGCCGTGGGTCATTTTTGTTTTTTTTTTCATTGgttttttgtttttt                                                                                                                                                                        >  2:655119/1‑250 (MQ=255)
                                                                                 ggCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGGACGGGGGTGTTTATGGGGGCTGGCAAAACGATTAGCCTTTCCGGGCCGGCGATCATTTTCGTTTTTATTATCATTGGTTTTTTGCTCttt                                                                                                                                                                        >  1:390324/1‑250 (MQ=255)
                                                                                  gCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCtttt                                                                                                                                                                       <  2:118676/250‑1 (MQ=255)
                                                                                   ccGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCttttt                                                                                                                                                                      <  1:107073/250‑1 (MQ=255)
                                                                                   ccGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGGACGGGGGTGTTTATGGGGTCTGGCCAAACGATTAGCCTTGCCCGGCCGGCGATCATTTTCGTTTTTATGATTATTGGGTTTATGCTCttttt                                                                                                                                                                      >  1:224822/1‑250 (MQ=255)
                                                                                    cGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGCGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTc                                                                                                                                                                     <  1:162768/250‑1 (MQ=255)
                                                                                      tAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTAAGGGGGTGGTTATGGGGGCTGTCAAAACGATTAGCCTTGCCGGGCCGTCGGTCATTTTTGTTTTTATGTTCATTGGTTTTTTTCTCTTTTTCGt                                                                                                                                                                   >  1:559518/1‑250 (MQ=255)
                                                                                            cTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGTACGGGGGTGTTTTTGGGGTCTGGCAAAACGATTAGCCTTTGCGGGGCGGCGATCATTTTCTTTTATATGATCTCTGGTTTTTTGTTCTTTTTTGTGATTCg                                                                                                                                                             >  2:363332/1‑250 (MQ=255)
                                                                                               aacaacACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCgg                                                                                                                                                            <  1:588959/248‑1 (MQ=255)
                                                                                               aacaacACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGCTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGc                                                                                                                                                          <  1:303709/250‑1 (MQ=255)
                                                                                                     acagacagGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGGACGGGGGTGTTTATGGGGTCTGGCAAAAAGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTTTGCTCTTTTTTTTGATGCGGGGGATggg                                                                                                                                                    >  1:162581/1‑250 (MQ=255)
                                                                                                      aagacagGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATgggg                                                                                                                                                   <  2:94014/249‑1 (MQ=255)
                                                                                                                    aagaaAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTc                                                                                                                                     <  2:621667/250‑1 (MQ=255)
                                                                                                                         aaaaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGGTAGGGGGTGTTTTTGGGGTGTGGAAAAACGATTAGCCTTTCCGGGGCGTGTGTATTTTTTGTTTATATGATCATTGGTTTTTTGTGCTTTTTTGTGTTGCGGGCAATGGGGGGATTGCTTCTTTTTAATc                                                                                                                                >  2:48390/1‑250 (MQ=255)
                                                                                                                           aaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTg                                                                                                                              <  1:131234/250‑1 (MQ=255)
                                                                                                                             aCATGTTAGAGCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGa                                                                                                                            <  2:289111/250‑1 (MQ=255)
                                                                                                                               aTGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAAt                                                                                                                          <  1:531201/250‑1 (MQ=255)
                                                                                                                                  gTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACa                                                                                                                       <  1:246672/250‑1 (MQ=255)
                                                                                                                                     gATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAAt                                                                                                                    <  1:85101/250‑1 (MQ=255)
                                                                                                                                           gTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTTGGACGGGGGTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATTATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGGATTGCTGCTTTTGAATCTGGAATACAAATCttttt                                                                                                              >  1:328911/1‑249 (MQ=255)
                                                                                                                                              aaaGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTg                                                                                                           <  2:20342/250‑1 (MQ=255)
                                                                                                                                               aaGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTTGTGa                                                                                                          >  1:260901/1‑250 (MQ=255)
                                                                                                                                                  tCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACtt                                                                                                       <  2:460819/250‑1 (MQ=255)
                                                                                                                                                     ttGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGTTCGGGGGTGTTTTTTGGGGCTGGCAAAAAGATTAGCCTTGCCGGGGCGTCGGTCATTTTTGTTTTTATTATCATTGGTTTTATGCTCTTTTTTGTTATGCGGGGAATGGGGGAATTGTTGTTTTCTGATCTGGAATACAAAACTTTTTTTGGCTTCGc                                                                                                    >  2:307485/1‑250 (MQ=255)
                                                                                                                                                                 aGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTAc                                                                                         <  2:116969/249‑1 (MQ=255)
                                                                                                                                                                 aGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGCGCCCTTTGTACGGGGGTGTTTTTGGGGGCTGGCCAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTTTGCTCTTTTTTGTGATGCGGGCAATGGGGGGATTGCTGCCTTCGCATCTGGTATACCAATCTTTTAGTGACTTCTGTTCCGATTTACt                                                                                        >  2:16700/1‑250 (MQ=255)
                                                                                                                                                                                aGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTTTGGGGTCTGGCAAAACGATTAGCCTTGCCGGGGCGTCGCTCATTTTTGTTTATATGATCATTGGTTTTTTGCTCTTTTTTGTGATGCGGGCAATGGGGGGATTGCTGCTTTTGAATCTGGGATACAAATCTTTTAGTGACTTCGCTTTCGATTTACTCGGGGCGTGGGGAgg                                                                         >  1:648002/1‑250 (MQ=255)
                                                                                                                                                                                  tCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGGGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGat                                                                       <  2:300246/250‑1 (MQ=255)
                                                                                                                                                                                    gCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCTAAAAGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGGTTTTTGCTCTTTTTTGTGATGCGGGCAATGGGGGGATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGGGGGCAGGatat                                                                     >  1:647314/1‑250 (MQ=255)
                                                                                                                                                                                         ggCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCAc                                                                <  2:126543/250‑1 (MQ=255)
                                                                                                                                                                                          gcgcAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCAcc                                                               <  1:60719/250‑1 (MQ=255)
                                                                                                                                                                                            gcAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCgg                                                             <  2:433960/250‑1 (MQ=255)
                                                                                                                                                                                                 ctcACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGTACGGGGTTTTTTATGGGGTTTGGCAAAACGGTTAGCCTTGCCGGGCCGTCGGTCCTTTTTGTTTTTATGATCATTGGGTTTTTGTTCTTTTTCGTGATTTGGGGCATGGGGGGATTGCTGCTTTCGGATTTGGGATAAAAATATTTTTTTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCTGGATGTTTTACCGGccg                                                          >  2:440294/1‑246 (MQ=255)
                                                                                                                                                                                                   cacaAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGTACGGGGGTGTTTATGGGGTCTGGCCAAAAGCTTAAGCCTGCCCGGCCGTCGGTCATTTTCGTTTTTATGATTCTTGGGTTTTTTTTCTTTTTTTGGATTTGGGCAATGGGGGGATTTCTGGTTTCTAATTTGGGATTTAAATATTTTTGTGTCTTTGCTTCCGCTTTACTCGGGCCGGGGGCAGGTGGTTTCACCGGCGGGGCt                                                      >  2:399114/1‑250 (MQ=255)
                                                                                                                                                                                                     caAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTa                                                    <  1:48390/250‑1 (MQ=255)
                                                                                                                                                                                                         ccGACATATTCAGCTTATTGCCATTGGCGGTGCCATTTGTACGGGGGTGTTTTTTGGGTGTTGCCAAACGATTATCCCTTCCGGGGCGGCGGTTATTTTTGTTTTTTTGATCATTGGTTTTTTGCTCTTTTTCGTGATTCGGGCAATGGGGGGATTTCTGCTTTCTGATCTGGAATACAAAACTTTTTGTGACTTCGCTTCCGGTTTACTCGGGGCGTGGGCAGGATATTTTACCGGCTGGGCTTTCTgg                                                >  2:256813/1‑250 (MQ=255)
                                                                                                                                                                                                          cGACATATTCAGCTTATTGCCATTGGCGGTGCCATTTGTACGGGGGTGTTTATTGGGTCTTGCAAAAAGATTAGCCTTTCCGGGCCGTCGATCCTTTTCTTTTTTATGATTATTGGGTTTTTGCTCTTTTTTGTGATGCGGGCCATGGGGGGATTGCTGCTTTTGGATCTGGGATACAAATTTTTTATGTAATTCGCTTCCGATTTAATCGGGCCGTGGGCGGGATATTTCACCGGCTGGGATTATTggg                                               >  2:498765/1‑249 (MQ=255)
                                                                                                                                                                                                          cGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGGCTTACTGGt                                               >  1:414580/1‑250 (MQ=255)
                                                                                                                                                                                                                 ttCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTctgct                                         <  1:363332/249‑1 (MQ=255)
                                                                                                                                                                                                                            gCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAAcc                              <  1:633995/249‑1 (MQ=255)
                                                                                                                                                                                                                             ccATTGGCGGTGCCATTGGTACGGGGTTTTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCgg                            <  2:393387/250‑1 (MQ=255)
                                                                                                                                                                                                                                               ggtcGGGGTTTGTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGtggtgg           <  2:258541/246‑1 (MQ=255)
                                                                                                                                                                                                                                                 aCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGAGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGGCGTGGTGGCGa        >  2:147292/1‑250 (MQ=255)
                                                                                                                                                                                                                                                  cGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGAt       <  1:211656/250‑1 (MQ=255)
                                                                                                                                                                                                                                                   ggggTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATc      <  1:313760/250‑1 (MQ=255)
                                                                                                                                                                                                                                                       ttgttTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACgg  <  2:646370/250‑1 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                         
AATCTTAGTATTCATCCCGCGTATTGTTACCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGG  >  NZ_CP009273/4419554‑4420050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑