Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,145,154	0	T	G	37.1%	81.3 / 13.0	65	intergenic (+27/+38)	yegJ/pphC	DUF2314 domain‑containing protein/protein‑serine/threonine phosphatase PphC
Reads supporting (aligned to +/- strand):  ref base T (19/20);  new base G (0/23);  total (19/46)
Fisher's exact test for biased strand distribution p-value = 2.61e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCG  >  NZ_CP009273/2144909‑2145400                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                              aCTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGc                                                                                                                                                                                                                                                            <  1:420848/249‑1 (MQ=255)     aTTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑Gtt                                                                                                                                                                                                                                                       <  1:640243/249‑1 (MQ=255)     aTTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑Gt                                                                                                                                                                                                                                                        <  1:937291/248‑1 (MQ=255)      ttCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTg                                                                                                                                                                                                                                                      <  2:7102/249‑1 (MQ=255)              gCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTTTATCATTTTTTCTTTTTTTTTTTCCTATCCCAAAAAAAAAACCGAACAGCCAAAAGCAAAAATAAATTTCTTAT‑CTACCG‑TTATATTTTTTTTTTTAAA‑TTGCG‑GGTggtgtttttt                                                                                                                                                                                                                                             >  2:93184/1‑241 (MQ=255)              gCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑Ttg‑ttgt                                                                                                                                                                                                                                           <  1:791385/250‑1 (MQ=255)                 tGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTTTATCATTTATCCTTTTTTTGTTACCTATCCCAAAAAAAAAACCGACCAGCCAAGAGCAAAAATAATTTGCTAAT‑CAACCG‑TTATATTTTTTTTTTTAAA‑TTGCG‑GGTGGA‑ttt‑ttttttt                                                                                                                                                                                                                                        >  1:339857/1‑245 (MQ=255)                   tttATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTT‑C‑a                                                                                                                                                                                                                                    <  2:625855/250‑1 (MQ=255)                          gCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGcc                                                                                                                                                                                                                             <  1:457744/249‑1 (MQ=255)                                tCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATAACATTTATCCTTTTTTTGTTACCTATTCCAAAAAAAAAAACGACGAGCTAAAAAGAAAAATAATTTGCTAAT‑AAACCGTTTTTTTTTTTTTTTTAAAT‑TGCGG‑GTGGAA‑TTT‑TTTTTTT‑T‑AA‑Cccccctaga                                                                                                                                                                                                                        >  1:234306/1‑244 (MQ=255)                                 cAGAAATGCCCCTGGTATTGTAAAAAACGTAGAATAGGGCAAGAAGTGAAAAAAAAAAAAAAAAGATTTTTCAGACTGGATGTATACGCGGGGGAATAAAATTT‑ACGGCGTTTATACCATTGATCTTTTATTGGTCCCCTATCAAAAAGAAGAAGCCGACGAGTTAAGAGAAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑GGGGG‑TTTGAA‑TTT‑TTGTCTT‑C‑AA‑CCGCCATGAGaaa                                                                                                                                                                                                                     <  2:346378/250‑1 (MQ=255)                                               ttATTGTTAAAAACGTAAAATAGGGCCAGGAGTGAAAAATCAAAAAAGAAGATATTTCAGATGGGATGTATCCGCGGGGGGATAAAATTT‑ACGGCGGTTATACCTTTAATCTTTTATTGGCCCCCTATCAAAAAGAAGAAGCCGACGAGCTAAGAGAAAAATTAGTCCGCTAAC‑CAGCCT‑TTATATTTATTTTTTAAAC‑GGGCG‑GTTGAT‑TTG‑TTTTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGccc                                                                                                                                                                                                         <  1:885877/247‑1 (MQ=255)                                                       aaaaaggaaaaaTAGGGCAAGGAGGGAAAAATAAAGAAAAAAGATATTTCAGACGGGATGTATACGCGGGGGAAAAAAATTT‑ACGGCGTTTATCCCTTTGATCTTTTATTGGTCCCCTACCAAAAAAAAGAAGCCGACGAGTAAAGAGAAAAATTAGTTCGCTAAT‑CAGCCT‑TTATATTTTTTGTTAAAAC‑GGGCG‑TTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAg                                                                                                                                                                                               <  1:274615/240‑1 (MQ=255)                                                       aCAAACGTAAAATAGGGCCAGGAGTGAAAAATCAAAAAAAAAAATATTTCAGACTGGATGTATCCGCGGGGGAAAAAAATTT‑ACGGGGTTTATCCCTTTTATCTTTTATTGGTCCCCTATCCAAAAGAAGAAGCCGACGAGCAAAGAGAAAAATTATTTCGCTAAT‑CAGCCG‑TTATTTTTATTTTTTAAAC‑TGGCG‑GTTAAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAg                                                                                                                                                                                               <  1:788060/250‑1 (MQ=255)                                                        cAAACGTAGAATGGGGCCGGGAGTGAAAAACAAAAAAAGAAAATATTTCAGACTGGATGTATCCGCGGGGGGATAAAATTT‑ACGGCGGTTATACCTTTGATCTTTTATTGGTCCCCTATCAAAAAGAAGAACCCGACGAGAAAAGAGAAAAATTGGTTCGCTAAT‑CAGCCG‑TTATATTTATTTTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTT‑A‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGt                                                                                                                                                                                              <  1:865507/250‑1 (MQ=255)                                                               aGAATAGGGCAAGGAGTGAAAAAAAAAAAAAAAAGATATTTCAGATGGGATGTATCCGGGGGGGAAAAAAATTT‑CGGGGGTTTATCCCATTTATCTTTTATTGGTCCCCTAACAAAAAGAAGAAGCCGAGGAGCAAAGAGAAAAATTAGTTCGCTAAT‑CGGCCT‑TTATTTTTATTGTTAAAAC‑GGGCG‑GTTAAT‑TTT‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACg                                                                                                                                                                                       <  2:306955/250‑1 (MQ=255)                                                                     gggCCAGGAGTGAAAAATCAAAAAAGAAGATATTTCAGACTGGATGTATCCGCGCGGGGATAAAATTT‑CCGGCGGTTATACCTTTGATCCTTTATTGGTCCCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCT‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTa                                                                                                                                                                                  <  1:305662/248‑1 (MQ=255)                                                                      ggCCGGGAGTGAAAAATAAAGAAAAAAGATATTTCAGACTGGATGTATACGCGGGGGAAAAAAATTT‑ACGGCGTTTATACCTTTGATCTTTTATTGGTCCCCTATCAAAAAAAAGAAGCCGACGAGCAAAGAGAAAAATTAGTTCGCTAAT‑CAGCCG‑TTATTTTTATTTTTAAAAC‑TGGCG‑TTTGAT‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatc                                                                                                                                                                                <  1:98006/250‑1 (MQ=255)                                                                                  aaaaaaaaaaaagaaGATATTTCAGACTGGATTTATCCGCGGGGGGAAAAAATTT‑CCGGCGTTTATACCTTTGATCTTTTATTGGTCCCCTACCAAAAAAAAGAAGCCGACGAGCAAAGAGAAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTTTTGTTTAAAC‑TGGCG‑GTTGAA‑TTT‑TTGTCTT‑A‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACg                                                                                                                                                                    <  1:172017/241‑1 (MQ=255)                                                                                      caaaaaaaaaaGTTTTTTCAGCCGGGTTGTATCCGCGGGGGAAAAAAATTG‑AGGGCGGTAACACTTTTGATCTTTTATTGTCCCCCTACCAAAAAGAAGAAGCCGACGAGAAAAGAAAAAAATTAGTCCTCAAAT‑CAGCCT‑TTATTTTTTTTTTTAAAAC‑GGGGG‑TTTGAT‑TTT‑TTTTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCg                                                                                                                                                                <  2:522690/245‑1 (MQ=255)                                                                                                     ttacAGACTGGTTGTTACCGCGGGGGAATAAAATTT‑AGGGGGTTTAACCTTTTGTTCTTTTATGGGCCACCTAACAAAAAGAAGAAGCCAACGAGAAAAAAAAAAATTTATTTCGCTAAC‑CACCCT‑TTATTTTTTTTTTTAAACC‑GGGCG‑TTTAAT‑TTT‑TTGTCTA‑C‑AC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCt                                                                                                                                                  <  2:185763/246‑1 (MQ=255)                                                                                                          gACGGGTTGTATACGCGGGGGAAAAAAATTT‑CCGGCGGTTATCCCTTTGTTCTTTTTTTGGTCCCCTACCAAAAAAAAAAAGCCGAGGAGCAAAGAAAAAAATTAGTTCGCTAAT‑CAGCCT‑TTATTTTTTTTTTTAAAAC‑TGGGG‑TTTAAA‑TTT‑TTGTCTT‑A‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACaa                                                                                                                                            <  1:401135/250‑1 (MQ=255)                                                                                                           acgggATGTATACGCGGGGGAATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCAAAAAAAAGAAGCCGACGAGCAAAGAAAAAAATTAGTTCGCTAAC‑CAGCCT‑TTATATTTTTTGTTAAAAC‑GGGGG‑GTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACaaa                                                                                                                                           <  1:666117/247‑1 (MQ=255)                                                                                                               gATGTATACGCGGGGGAAAAAAATTT‑ACGGCGTTTATCCCTTTGATCTTTTATGGGTCACCTACCAAAAAAAAGAACCCGACGAGCAAAGAAAAAAATTAGTTCGCTAAT‑CAGCCT‑TTATATTTATTGTTAAAAC‑TGGCG‑TTTGAT‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACt                                                                                                                                       <  1:552510/250‑1 (MQ=255)                                                                                                                        cggggtgaaaaaatTTT‑ACGGCTGTATTCCTATTTTTCTTTTTTTGGCCCCCTATAAAAAAGAAGAGGCCGACGAGTAAAAAGAAAATTTTGTTC‑ATAATGCGGCTT‑TTTTTTTTATTTTTTAACC‑GGGCG‑GTTTAT‑TTG‑TTATCTA‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACa                                                                                                                              <  2:889170/240‑1 (MQ=255)                                                                                                                         gcgggggAAAAAATTTGACGGTGATTATACCATTTATCTTTTATTGATCACCTACCAAAAAGAAGAAGCCGAGAAAAAAAAAAAAAAATTATTCCCCTAAC‑CAGCTT‑TTATTTTTTTTG‑TTAAAA‑GGGGG‑GTTGAA‑TTT‑TTTTTTT‑CAAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACa                                                                                                                              <  2:154656/247‑1 (MQ=255)                                                                                                                           gggggATAAAATTT‑ACGGGGGTTATCCCTTTGATCTTTTTTTGGTCCCCTATCAAAAAAAAAAAGCCGACGAGTTAAGAAAAAAATTATTTCGCTAAT‑CAGCCG‑TTATATTTATTTTTAAAAC‑GGGGG‑TTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAAtt                                                                                                                           <  1:703846/249‑1 (MQ=255)                                                                                                                                    aaTTT‑ACGGAGGTTATACATTTGATCCTTTATGGGTCACCAATCAAAAAGAAAAGGCCAAAGAGCAAAGAGAAAATTTAGTTCGATAAT‑CAGCCT‑TTATATTTATTGTTAAAAC‑GGGCG‑GTTGAA‑TGG‑TTGTCTT‑A‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGtaata                                                                                                                  <  1:798132/250‑1 (MQ=255)                                                                                                                                     tttt‑AGGGCGTTTATACCATTAATCCTTTATTGGTCACCTATCAAAAAAAAAAGCCCGACGAGTTAAGAAAAAAATTATTTCGCTAAT‑CAGCCG‑TTATATTTTTTGTTTAACC‑TGGGG‑TTTGAA‑TTG‑TTTTCTC‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGtaataa                                                                                                                 <  1:737415/249‑1 (MQ=255)                                                                                                                                          ccggcgtTTATCCTTTTGATCTTTTTTTGGTCCCCTACCAAAAAAAAGAAGCCGACGAGCAAAGAGAAAAATTATTTCGCTAAT‑CAGCCT‑TTATTTTTTTTTTTAAACC‑GGGCG‑TTTGAT‑TTT‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATc                                                                                                              <  1:607010/248‑1 (MQ=255)                                                                                                                                                  tatcCCTTTGATCTTTTATTGGTCCCCTACCAAAAAAAAGAGCCCGAGGAGAAAAGAGAAAAATTAGTCCTCTAAC‑CAGCCT‑TTATTTTTTTTTTTAAACC‑GGGCG‑TTTAAT‑TTT‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAgttgtt                                                                                                      <  1:493130/249‑1 (MQ=255)                                                                                                                                                       cATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTT‑TTTTCTT‑T‑AA‑CCCCCATGAGAAAATCACCCTGCCCCCAACGCCAGTGCCAACGTTTTTTCATCATCCGTACGCCCGCTTACCCCCCGGCTGGGCAAAAAATGCTTTAACAATTCCGGTAATAAATTGAGTTGTTTCTGCg                                                                                                >  2:453155/1‑250 (MQ=255)                                                                                                                                                          tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTTAA‑TTG‑TTGTTTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAACGCCAGTGCCAAAGTTTTATTATCATCCGTACGCTCGTTTAACGCCGGGCCGGACAAAAACTGCTTTAACAATTCAGGTAATAAAACGAGTTGTTTCTTCGttt                                                                                             >  2:937216/1‑249 (MQ=255)                                                                                                                                                          tGACCCTTTATGGGCCCCCTACCAAAAAAAAGAGCCCGACAAGCAAAAAGAAAATTTATTTCGCTAAT‑CGGCCT‑TTATATTTATTTTTTAAAC‑TGGGG‑TTTAAT‑TTG‑TTGTCTC‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGtt                                                                                              <  1:229380/249‑1 (MQ=255)                                                                                                                                                           gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGGA‑TTT‑TTGTCTT‑C‑AA‑CCGCCCAGTGAAAATTTCTCTGCCCACCGCGCCAGTGCCAAAGTTTTATTATCATCCGTACGCCCGTTTACCGCCCGGCCGGACCAAAAACGCTTTAACAATTCAGGTAATAAATCGAGTTTTTCCTGCCTt‑gc                                                                                           >  2:535991/1‑250 (MQ=255)                                                                                                                                                            aTCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTT‑TTGTCTT‑T‑TA‑CCGCCCTGAGAAAATCACTCTGCCCACAGCGCCAGTGCCCACGTTTTATCATAATCCGGACGCTCGTTTACCCCCGGGCCGGACAAAAAATGCTTTAAAAATTCAGGGAATAAATCGAGGTGTTCCTGCGGt‑gct                                                                                          >  2:212040/1‑250 (MQ=255)                                                                                                                                                               cTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTTAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCCTGAGAAAATCACTCTGCCCACAGCGCCAGGGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTTACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTgcc                                                                                       >  2:187068/1‑250 (MQ=255)                                                                                                                                                                tttATTGTTCACATACAAAAAAAAAGAGACCGAGGGGATAAGAGAAAAATTATTTCGTTACC‑CTCCTT‑TTATATTT‑TTTTTTAAAC‑GGGGGTGTTAAT‑TTG‑TTTTCTT‑C‑AC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTgcc                                                                                       <  2:862873/249‑1 (MQ=255)                                                                                                                                                                 ttATTGGCCCCCTACCAAAAAAAAGAAGCCAACGAGCTAAGAGAAAAATTATTTCGCTAAT‑CAGCCT‑TTATTTTTTTTTTTAAAAC‑GGGCG‑GTTAAA‑TTG‑TTTTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTgccg                                                                                      <  1:588244/249‑1 (MQ=255)                                                                                                                                                                      ggacacttaccaaaaaaaAGAGGCCGACGAGAAAAGAGAAAAATTGGTCCGATAAT‑CGGCGT‑TTATTTTTTTTTTTAAACC‑GGGCG‑GTTAAT‑TTT‑TTGTCTT‑A‑AC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGt                                                                                 <  1:967687/237‑1 (MQ=255)                                                                                                                                                                       tccACCTAACAAAAAAAAAAAGCCGACGACATAAGAGAAATATTAGTTCGTTAAT‑CATCTT‑TTTTTTTTATTTTTTAAGC‑GGGGG‑GTTGAA‑TT‑‑TTTTCTT‑C‑AACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTcc                                                                               <  2:788841/248‑1 (MQ=255)                                                                                                                                                                       gtccccaaccaaaaaaaaaaaGCCGAAGAGCAAAGAAAAAAATTATTCCTCAAAT‑CAGCCT‑TTATTTTTTTTTTTAAACC‑GGGCG‑TTTAAT‑TTT‑TTGTCTC‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTcc                                                                               <  1:715469/239‑1 (MQ=255)                                                                                                                                                                         cACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCCCCATGAGAAAATCACTCTTCCCCCAGCGCCAGTGCCCACGGTTTTTCCTCATCCGTACGCCCGTTTACCGCCCGGCTGGACAAAAAATGCTTTAAACATTCAGGGACTAAATACAGGTGTTTCTGCGTT‑GGTGCCCCCAGTCCAt                                                                             >  2:306988/1‑250 (MQ=255)                                                                                                                                                                                  aaaaGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGTA‑TTG‑TTGTCTT‑T‑AA‑ACGCCATGAGAAACACACTCTGCCCACAGCGCCACTGCCCACGGTTTTTTATAATCCCCACGCCCGTTTAACGCCGGGCTGGACAAAAAATGCTTTTACAATTCCGGTAATAAATCGAGGTGTTCCTGCGTT‑TCTGCCGCCCATCCATTTaaaaaag                                                                    >  2:722512/1‑248 (MQ=255)                                                                                                                                                                                       aagaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAAAAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAAAGGGGt                                                                >  1:145425/1‑249 (MQ=255)                                                                                                                                                                                             ccgcgaaGCAAAGAGAAAAATTATTCCGCAAAC‑CAGCTT‑TTAT‑TTTTTTTTTTAAAC‑CGGGG‑GTTTAATTTT‑TTTTCTC‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTaaagaaa                                                         <  1:484573/244‑1 (MQ=255)                                                                                                                                                                                             ccgccggcatAAGAAAAAAATTGGTTGGTTAAC‑CAGCCT‑TTTTTTTTATT‑TTTAAACTTGGCG‑TTTGAG‑TTG‑TTTTCTC‑C‑AC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTaaagaaa                                                         <  1:530625/241‑1 (MQ=255)                                                                                                                                                                                                acgaGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGGAAAGAAAgg                                                       >  1:91553/1‑249 (MQ=255)                                                                                                                                                                                                    gcaaaGAGAAAAATTATTTCGCTAAT‑CAGCCT‑TTATTTTTTTTTTTAAACC‑TGGCG‑TTTAAT‑TTT‑TTTTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACAt                                                  <  1:890235/247‑1 (MQ=255)                                                                                                                                                                                                        agagAAAAATTAGTTCGTTAAC‑CGGCTG‑TTATTTTTTTTGTTTAACC‑GGGGG‑GTTAAT‑TTT‑TTTTCTA‑A‑AC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgagg                                              <  1:455869/250‑1 (MQ=255)                                                                                                                                                                                                               aaTTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCCTGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCCTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTTACAATTTAGGTAATAAATCGAGTTGTTCCTGCGGT‑GCTGCCGCCAGTCCATTGAAAAACGGGGGAAAGAAAGGCACATGAGGAGAATTa                                       >  1:815528/1‑250 (MQ=255)                                                                                                                                                                                                                 ttAGCTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTGCTGGTCTC‑C‑AA‑CCCCCA‑GAGTAAATCACTCCGGCCACAACGGCAGGGCCAACGCTTTTTTAACATCCCTGTGGTCCCTTACCGCCGGGGCGGGGAAAAAAAGGTTTTACAACTTAGGGAATAAATAGTGGTGGTCCTTCGTTGGCTG‑CGCCAGGTCCTTGAAAAAAGGGGGAAATAAAGGCATATGAGGGGAAATATc                                     >  2:590504/1‑250 (MQ=255)                                                                                                                                                                                                                   attccGCTAAC‑CAGCTG‑TTATATTTATTTTTAAACC‑TGGCG‑TTAGAT‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATcc                                    <  1:675982/245‑1 (MQ=255)                                                                                                                                                                                                                          tAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCCCAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATAtt                            >  1:950077/1‑250 (MQ=255)                                                                                                                                                                                                                           aaaccacctg‑TTATTTTTTTTTTAAAAAC‑TGGGG‑GTTAAA‑TTT‑TTTTTTC‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCata                              <  1:127224/239‑1 (MQ=255)                                                                                                                                                                                                                            aaaccatcttttATATTTTTTGTTAAAAC‑GGGGG‑TTTGAT‑TTG‑TTGTCTA‑A‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATAtt                            <  1:92991/240‑1 (MQ=255)                                                                                                                                                                                                                                       tataTTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTT‑TTGTCTT‑T‑AA‑CCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTTGACAAAAAATGCTTTAACAATTTAGGTAATAAATCGAGTTGTTTCTGCGTT‑GCTTCCGCCAGTCCCTTGAAAAAAGGGGTAAAGAAAGGCACATGAGGGGAATTATCCAGCATATTTAAAGCCAggc                 >  2:611500/1‑248 (MQ=255)                                                                                                                                                                                                                                            tttttttttAAAC‑CGGGC‑GTTTAA‑TTT‑TTTTCTTAC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCt              <  1:229046/243‑1 (MQ=255)                                                                                                                                                                                                                                             ttttttttAAAA‑CGGGG‑GTTTAATTTG‑TTGTTTT‑A‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTg             <  1:850912/244‑1 (MQ=255)                                                                                                                                                                                                                                             tattTTTAAACC‑GGGGG‑GTTAAT‑TTT‑TTTTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTgg            <  1:93184/249‑1 (MQ=255)                                                                                                                                                                                                                                              attGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTTTT‑T‑AA‑CCGCCAAGAGAAAATCACTCTGCCCACAGCGCCAGGGCCAACGTTTTTTCATCATCCGTACGCTCGTTTACCCCCCGGCTGGACAAAAAATGCTTTAACAAATCAGGGAATAAAACGAGTTGTTTCTGCGTT‑GCTGCCGCCCGTCCATTGAAAAACGGGGGAAAGAAAGGCACATGAGGAGAATTATCCAGAATATTTAACGCCAGTCGCTggg           >  2:352666/1‑248 (MQ=255)                                                                                                                                                                                                                                              attGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGGAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGGAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGa           >  1:918683/1‑249 (MQ=255)                                                                                                                                                                                                                                                ttttaaaaC‑GGGGG‑TTTGAT‑TTG‑TTGTCTC‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATa         <  1:330860/244‑1 (MQ=255)                                                                                                                                                                                                                                                  ttaaaaC‑GGGCG‑TTTGAT‑TTT‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATAcc       <  1:186950/246‑1 (MQ=255)                                                                                                                                                                                                                                                      aaC‑TGGCG‑GTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCg  >  2:776696/1‑250 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                              ACTTATTCTAACGGCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCG  >  NZ_CP009273/2144909‑2145400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑