BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,145,167	0	G	T	38.7%	53.4 / 16.9	63	intergenic (+40/+25)	yegJ/pphC	DUF2314 domain‑containing protein/protein‑serine/threonine phosphatase PphC
Reads supporting (aligned to +/- strand): ref base G (16/22); new base T (5/19); total (21/42)
Fisher's exact test for biased strand distribution p-value = 1.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTG  >  NZ_CP009273/2144922‑2145409

gCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑Ttg‑ttgt                                                                                                                                                                                                                                                    <  1:791385/250‑1 (MQ=255)

   tGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTTTATCATTTATCCTTTTTTTGTTACCTATCCCAAAAAAAAAACCGACCAGCCAAGAGCAAAAATAATTTGCTAAT‑CAACCG‑TTATATTTTTTTTTTTAAA‑TTGCG‑GGTGGA‑ttt‑ttttttt                                                                                                                                                                                                                                                 >  1:339857/1‑245 (MQ=255)

     tttATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTT‑C‑a                                                                                                                                                                                                                                             <  2:625855/250‑1 (MQ=255)

            gCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGcc                                                                                                                                                                                                                                      <  1:457744/249‑1 (MQ=255)

                  tCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATAACATTTATCCTTTTTTTGTTACCTATTCCAAAAAAAAAAACGACGAGCTAAAAAGAAAAATAATTTGCTAAT‑AAACCGTTTTTTTTTTTTTTTTAAAT‑TGCGG‑GTGGAA‑TTT‑TTTTTTT‑T‑AA‑Cccccctaga                                                                                                                                                                                                                                 >  1:234306/1‑244 (MQ=255)

                   cAGAAATGCCCCTGGTATTGTAAAAAACGTAGAATAGGGCAAGAAGTGAAAAAAAAAAAAAAAAGATTTTTCAGACTGGATGTATACGCGGGGGAATAAAATTT‑ACGGCGTTTATACCATTGATCTTTTATTGGTCCCCTATCAAAAAGAAGAAGCCGACGAGTTAAGAGAAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑GGGGG‑TTTGAA‑TTT‑TTGTCTT‑C‑AA‑CCGCCATGAGaaa                                                                                                                                                                                                                              <  2:346378/250‑1 (MQ=255)

                                 ttATTGTTAAAAACGTAAAATAGGGCCAGGAGTGAAAAATCAAAAAAGAAGATATTTCAGATGGGATGTATCCGCGGGGGGATAAAATTT‑ACGGCGGTTATACCTTTAATCTTTTATTGGCCCCCTATCAAAAAGAAGAAGCCGACGAGCTAAGAGAAAAATTAGTCCGCTAAC‑CAGCCT‑TTATATTTATTTTTTAAAC‑GGGCG‑GTTGAT‑TTG‑TTTTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGccc                                                                                                                                                                                                                  <  1:885877/247‑1 (MQ=255)

                                         aaaaaggaaaaaTAGGGCAAGGAGGGAAAAATAAAGAAAAAAGATATTTCAGACGGGATGTATACGCGGGGGAAAAAAATTT‑ACGGCGTTTATCCCTTTGATCTTTTATTGGTCCCCTACCAAAAAAAAGAAGCCGACGAGTAAAGAGAAAAATTAGTTCGCTAAT‑CAGCCT‑TTATATTTTTTGTTAAAAC‑GGGCG‑TTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAg                                                                                                                                                                                                        <  1:274615/240‑1 (MQ=255)

                                         aCAAACGTAAAATAGGGCCAGGAGTGAAAAATCAAAAAAAAAAATATTTCAGACTGGATGTATCCGCGGGGGAAAAAAATTT‑ACGGGGTTTATCCCTTTTATCTTTTATTGGTCCCCTATCCAAAAGAAGAAGCCGACGAGCAAAGAGAAAAATTATTTCGCTAAT‑CAGCCG‑TTATTTTTATTTTTTAAAC‑TGGCG‑GTTAAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAg                                                                                                                                                                                                        <  1:788060/250‑1 (MQ=255)

                                          cAAACGTAGAATGGGGCCGGGAGTGAAAAACAAAAAAAGAAAATATTTCAGACTGGATGTATCCGCGGGGGGATAAAATTT‑ACGGCGGTTATACCTTTGATCTTTTATTGGTCCCCTATCAAAAAGAAGAACCCGACGAGAAAAGAGAAAAATTGGTTCGCTAAT‑CAGCCG‑TTATATTTATTTTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTT‑A‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGt                                                                                                                                                                                                       <  1:865507/250‑1 (MQ=255)

                                                 aGAATAGGGCAAGGAGTGAAAAAAAAAAAAAAAAGATATTTCAGATGGGATGTATCCGGGGGGGAAAAAAATTT‑CGGGGGTTTATCCCATTTATCTTTTATTGGTCCCCTAACAAAAAGAAGAAGCCGAGGAGCAAAGAGAAAAATTAGTTCGCTAAT‑CGGCCT‑TTATTTTTATTGTTAAAAC‑GGGCG‑GTTAAT‑TTT‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACg                                                                                                                                                                                                <  2:306955/250‑1 (MQ=255)

                                                       gggCCAGGAGTGAAAAATCAAAAAAGAAGATATTTCAGACTGGATGTATCCGCGCGGGGATAAAATTT‑CCGGCGGTTATACCTTTGATCCTTTATTGGTCCCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCT‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTa                                                                                                                                                                                           <  1:305662/248‑1 (MQ=255)

                                                        ggCCGGGAGTGAAAAATAAAGAAAAAAGATATTTCAGACTGGATGTATACGCGGGGGAAAAAAATTT‑ACGGCGTTTATACCTTTGATCTTTTATTGGTCCCCTATCAAAAAAAAGAAGCCGACGAGCAAAGAGAAAAATTAGTTCGCTAAT‑CAGCCG‑TTATTTTTATTTTTAAAAC‑TGGCG‑TTTGAT‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatc                                                                                                                                                                                         <  1:98006/250‑1 (MQ=255)

                                                                    aaaaaaaaaaaagaaGATATTTCAGACTGGATTTATCCGCGGGGGGAAAAAATTT‑CCGGCGTTTATACCTTTGATCTTTTATTGGTCCCCTACCAAAAAAAAGAAGCCGACGAGCAAAGAGAAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTTTTGTTTAAAC‑TGGCG‑GTTGAA‑TTT‑TTGTCTT‑A‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACg                                                                                                                                                                             <  1:172017/241‑1 (MQ=255)

                                                                        caaaaaaaaaaGTTTTTTCAGCCGGGTTGTATCCGCGGGGGAAAAAAATTG‑AGGGCGGTAACACTTTTGATCTTTTATTGTCCCCCTACCAAAAAGAAGAAGCCGACGAGAAAAGAAAAAAATTAGTCCTCAAAT‑CAGCCT‑TTATTTTTTTTTTTAAAAC‑GGGGG‑TTTGAT‑TTT‑TTTTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCg                                                                                                                                                                         <  2:522690/245‑1 (MQ=255)

                                                                                       ttacAGACTGGTTGTTACCGCGGGGGAATAAAATTT‑AGGGGGTTTAACCTTTTGTTCTTTTATGGGCCACCTAACAAAAAGAAGAAGCCAACGAGAAAAAAAAAAATTTATTTCGCTAAC‑CACCCT‑TTATTTTTTTTTTTAAACC‑GGGCG‑TTTAAT‑TTT‑TTGTCTA‑C‑AC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCt                                                                                                                                                           <  2:185763/246‑1 (MQ=255)

                                                                                            gACGGGTTGTATACGCGGGGGAAAAAAATTT‑CCGGCGGTTATCCCTTTGTTCTTTTTTTGGTCCCCTACCAAAAAAAAAAAGCCGAGGAGCAAAGAAAAAAATTAGTTCGCTAAT‑CAGCCT‑TTATTTTTTTTTTTAAAAC‑TGGGG‑TTTAAA‑TTT‑TTGTCTT‑A‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACaa                                                                                                                                                     <  1:401135/250‑1 (MQ=255)

                                                                                             acgggATGTATACGCGGGGGAATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCAAAAAAAAGAAGCCGACGAGCAAAGAAAAAAATTAGTTCGCTAAC‑CAGCCT‑TTATATTTTTTGTTAAAAC‑GGGGG‑GTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACaaa                                                                                                                                                    <  1:666117/247‑1 (MQ=255)

                                                                                                 gATGTATACGCGGGGGAAAAAAATTT‑ACGGCGTTTATCCCTTTGATCTTTTATGGGTCACCTACCAAAAAAAAGAACCCGACGAGCAAAGAAAAAAATTAGTTCGCTAAT‑CAGCCT‑TTATATTTATTGTTAAAAC‑TGGCG‑TTTGAT‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACt                                                                                                                                                <  1:552510/250‑1 (MQ=255)

                                                                                                          cggggtgaaaaaatTTT‑ACGGCTGTATTCCTATTTTTCTTTTTTTGGCCCCCTATAAAAAAGAAGAGGCCGACGAGTAAAAAGAAAATTTTGTTC‑ATAATGCGGCTT‑TTTTTTTTATTTTTTAACC‑GGGCG‑GTTTAT‑TTG‑TTATCTA‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACa                                                                                                                                       <  2:889170/240‑1 (MQ=255)

                                                                                                           gcgggggAAAAAATTTGACGGTGATTATACCATTTATCTTTTATTGATCACCTACCAAAAAGAAGAAGCCGAGAAAAAAAAAAAAAAATTATTCCCCTAAC‑CAGCTT‑TTATTTTTTTTG‑TTAAAA‑GGGGG‑GTTGAA‑TTT‑TTTTTTT‑CAAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACa                                                                                                                                       <  2:154656/247‑1 (MQ=255)

                                                                                                             gggggATAAAATTT‑ACGGGGGTTATCCCTTTGATCTTTTTTTGGTCCCCTATCAAAAAAAAAAAGCCGACGAGTTAAGAAAAAAATTATTTCGCTAAT‑CAGCCG‑TTATATTTATTTTTAAAAC‑GGGGG‑TTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAAtt                                                                                                                                    <  1:703846/249‑1 (MQ=255)

                                                                                                                      aaTTT‑ACGGAGGTTATACATTTGATCCTTTATGGGTCACCAATCAAAAAGAAAAGGCCAAAGAGCAAAGAGAAAATTTAGTTCGATAAT‑CAGCCT‑TTATATTTATTGTTAAAAC‑GGGCG‑GTTGAA‑TGG‑TTGTCTT‑A‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGtaata                                                                                                                           <  1:798132/250‑1 (MQ=255)

                                                                                                                       tttt‑AGGGCGTTTATACCATTAATCCTTTATTGGTCACCTATCAAAAAAAAAAGCCCGACGAGTTAAGAAAAAAATTATTTCGCTAAT‑CAGCCG‑TTATATTTTTTGTTTAACC‑TGGGG‑TTTGAA‑TTG‑TTTTCTC‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGtaataa                                                                                                                          <  1:737415/249‑1 (MQ=255)

                                                                                                                            ccggcgtTTATCCTTTTGATCTTTTTTTGGTCCCCTACCAAAAAAAAGAAGCCGACGAGCAAAGAGAAAAATTATTTCGCTAAT‑CAGCCT‑TTATTTTTTTTTTTAAACC‑GGGCG‑TTTGAT‑TTT‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATc                                                                                                                       <  1:607010/248‑1 (MQ=255)

                                                                                                                                    tatcCCTTTGATCTTTTATTGGTCCCCTACCAAAAAAAAGAGCCCGAGGAGAAAAGAGAAAAATTAGTCCTCTAAC‑CAGCCT‑TTATTTTTTTTTTTAAACC‑GGGCG‑TTTAAT‑TTT‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAgttgtt                                                                                                               <  1:493130/249‑1 (MQ=255)

                                                                                                                                         cATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTT‑TTTTCTT‑T‑AA‑CCCCCATGAGAAAATCACCCTGCCCCCAACGCCAGTGCCAACGTTTTTTCATCATCCGTACGCCCGCTTACCCCCCGGCTGGGCAAAAAATGCTTTAACAATTCCGGTAATAAATTGAGTTGTTTCTGCg                                                                                                         >  2:453155/1‑250 (MQ=255)

                                                                                                                                            tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTTAA‑TTG‑TTGTTTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAACGCCAGTGCCAAAGTTTTATTATCATCCGTACGCTCGTTTAACGCCGGGCCGGACAAAAACTGCTTTAACAATTCAGGTAATAAAACGAGTTGTTTCTTCGttt                                                                                                      >  2:937216/1‑249 (MQ=255)

                                                                                                                                            tGACCCTTTATGGGCCCCCTACCAAAAAAAAGAGCCCGACAAGCAAAAAGAAAATTTATTTCGCTAAT‑CGGCCT‑TTATATTTATTTTTTAAAC‑TGGGG‑TTTAAT‑TTG‑TTGTCTC‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGtt                                                                                                       <  1:229380/249‑1 (MQ=255)

                                                                                                                                             gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGGA‑TTT‑TTGTCTT‑C‑AA‑CCGCCCAGTGAAAATTTCTCTGCCCACCGCGCCAGTGCCAAAGTTTTATTATCATCCGTACGCCCGTTTACCGCCCGGCCGGACCAAAAACGCTTTAACAATTCAGGTAATAAATCGAGTTTTTCCTGCCTt‑gc                                                                                                    >  2:535991/1‑250 (MQ=255)

                                                                                                                                              aTCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTT‑TTGTCTT‑T‑TA‑CCGCCCTGAGAAAATCACTCTGCCCACAGCGCCAGTGCCCACGTTTTATCATAATCCGGACGCTCGTTTACCCCCGGGCCGGACAAAAAATGCTTTAAAAATTCAGGGAATAAATCGAGGTGTTCCTGCGGt‑gct                                                                                                   >  2:212040/1‑250 (MQ=255)

                                                                                                                                                 cTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTTAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCCTGAGAAAATCACTCTGCCCACAGCGCCAGGGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTTACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTgcc                                                                                                >  2:187068/1‑250 (MQ=255)

                                                                                                                                                  tttATTGTTCACATACAAAAAAAAAGAGACCGAGGGGATAAGAGAAAAATTATTTCGTTACC‑CTCCTT‑TTATATTT‑TTTTTTAAAC‑GGGGGTGTTAAT‑TTG‑TTTTCTT‑C‑AC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTgcc                                                                                                <  2:862873/249‑1 (MQ=255)

                                                                                                                                                   ttATTGGCCCCCTACCAAAAAAAAGAAGCCAACGAGCTAAGAGAAAAATTATTTCGCTAAT‑CAGCCT‑TTATTTTTTTTTTTAAAAC‑GGGCG‑GTTAAA‑TTG‑TTTTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTgccg                                                                                               <  1:588244/249‑1 (MQ=255)

                                                                                                                                                        ggacacttaccaaaaaaaAGAGGCCGACGAGAAAAGAGAAAAATTGGTCCGATAAT‑CGGCGT‑TTATTTTTTTTTTTAAACC‑GGGCG‑GTTAAT‑TTT‑TTGTCTT‑A‑AC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGt                                                                                          <  1:967687/237‑1 (MQ=255)

                                                                                                                                                         tccACCTAACAAAAAAAAAAAGCCGACGACATAAGAGAAATATTAGTTCGTTAAT‑CATCTT‑TTTTTTTTATTTTTTAAGC‑GGGGG‑GTTGAA‑TT‑‑TTTTCTT‑C‑AACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTcc                                                                                        <  2:788841/248‑1 (MQ=255)

                                                                                                                                                         gtccccaaccaaaaaaaaaaaGCCGAAGAGCAAAGAAAAAAATTATTCCTCAAAT‑CAGCCT‑TTATTTTTTTTTTTAAACC‑GGGCG‑TTTAAT‑TTT‑TTGTCTC‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTcc                                                                                        <  1:715469/239‑1 (MQ=255)

                                                                                                                                                           cACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCCCCATGAGAAAATCACTCTTCCCCCAGCGCCAGTGCCCACGGTTTTTCCTCATCCGTACGCCCGTTTACCGCCCGGCTGGACAAAAAATGCTTTAAACATTCAGGGACTAAATACAGGTGTTTCTGCGTT‑GGTGCCCCCAGTCCAt                                                                                      >  2:306988/1‑250 (MQ=255)

                                                                                                                                                                    aaaaGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGTA‑TTG‑TTGTCTT‑T‑AA‑ACGCCATGAGAAACACACTCTGCCCACAGCGCCACTGCCCACGGTTTTTTATAATCCCCACGCCCGTTTAACGCCGGGCTGGACAAAAAATGCTTTTACAATTCCGGTAATAAATCGAGGTGTTCCTGCGTT‑TCTGCCGCCCATCCATTTaaaaaag                                                                             >  2:722512/1‑248 (MQ=255)

                                                                                                                                                                         aagaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAAAAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAAAGGGGt                                                                         >  1:145425/1‑249 (MQ=255)

                                                                                                                                                                               ccgcgaaGCAAAGAGAAAAATTATTCCGCAAAC‑CAGCTT‑TTAT‑TTTTTTTTTTAAAC‑CGGGG‑GTTTAATTTT‑TTTTCTC‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTaaagaaa                                                                  <  1:484573/244‑1 (MQ=255)

                                                                                                                                                                               ccgccggcatAAGAAAAAAATTGGTTGGTTAAC‑CAGCCT‑TTTTTTTTATT‑TTTAAACTTGGCG‑TTTGAG‑TTG‑TTTTCTC‑C‑AC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTaaagaaa                                                                  <  1:530625/241‑1 (MQ=255)

                                                                                                                                                                                  acgaGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGGAAAGAAAgg                                                                >  1:91553/1‑249 (MQ=255)

                                                                                                                                                                                      gcaaaGAGAAAAATTATTTCGCTAAT‑CAGCCT‑TTATTTTTTTTTTTAAACC‑TGGCG‑TTTAAT‑TTT‑TTTTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACAt                                                           <  1:890235/247‑1 (MQ=255)

                                                                                                                                                                                          agagAAAAATTAGTTCGTTAAC‑CGGCTG‑TTATTTTTTTTGTTTAACC‑GGGGG‑GTTAAT‑TTT‑TTTTCTA‑A‑AC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgagg                                                       <  1:455869/250‑1 (MQ=255)

                                                                                                                                                                                                 aaTTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCCTGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCCTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTTACAATTTAGGTAATAAATCGAGTTGTTCCTGCGGT‑GCTGCCGCCAGTCCATTGAAAAACGGGGGAAAGAAAGGCACATGAGGAGAATTa                                                >  1:815528/1‑250 (MQ=255)

                                                                                                                                                                                                   ttAGCTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTGCTGGTCTC‑C‑AA‑CCCCCA‑GAGTAAATCACTCCGGCCACAACGGCAGGGCCAACGCTTTTTTAACATCCCTGTGGTCCCTTACCGCCGGGGCGGGGAAAAAAAGGTTTTACAACTTAGGGAATAAATAGTGGTGGTCCTTCGTTGGCTG‑CGCCAGGTCCTTGAAAAAAGGGGGAAATAAAGGCATATGAGGGGAAATATc                                              >  2:590504/1‑250 (MQ=255)

                                                                                                                                                                                                     attccGCTAAC‑CAGCTG‑TTATATTTATTTTTAAACC‑TGGCG‑TTAGAT‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATcc                                             <  1:675982/245‑1 (MQ=255)

                                                                                                                                                                                                            tAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCCCAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATAtt                                     >  1:950077/1‑250 (MQ=255)

                                                                                                                                                                                                             aaaccacctg‑TTATTTTTTTTTTAAAAAC‑TGGGG‑GTTAAA‑TTT‑TTTTTTC‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCata                                       <  1:127224/239‑1 (MQ=255)

                                                                                                                                                                                                              aaaccatcttttATATTTTTTGTTAAAAC‑GGGGG‑TTTGAT‑TTG‑TTGTCTA‑A‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATAtt                                     <  1:92991/240‑1 (MQ=255)

                                                                                                                                                                                                                         tataTTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTT‑TTGTCTT‑T‑AA‑CCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTTGACAAAAAATGCTTTAACAATTTAGGTAATAAATCGAGTTGTTTCTGCGTT‑GCTTCCGCCAGTCCCTTGAAAAAAGGGGTAAAGAAAGGCACATGAGGGGAATTATCCAGCATATTTAAAGCCAggc                          >  2:611500/1‑248 (MQ=255)

                                                                                                                                                                                                                              tttttttttAAAC‑CGGGC‑GTTTAA‑TTT‑TTTTCTTAC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCt                       <  1:229046/243‑1 (MQ=255)

                                                                                                                                                                                                                               ttttttttAAAA‑CGGGG‑GTTTAATTTG‑TTGTTTT‑A‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTg                      <  1:850912/244‑1 (MQ=255)

                                                                                                                                                                                                                               tattTTTAAACC‑GGGGG‑GTTAAT‑TTT‑TTTTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTgg                     <  1:93184/249‑1 (MQ=255)

                                                                                                                                                                                                                                attGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTTTT‑T‑AA‑CCGCCAAGAGAAAATCACTCTGCCCACAGCGCCAGGGCCAACGTTTTTTCATCATCCGTACGCTCGTTTACCCCCCGGCTGGACAAAAAATGCTTTAACAAATCAGGGAATAAAACGAGTTGTTTCTGCGTT‑GCTGCCGCCCGTCCATTGAAAAACGGGGGAAAGAAAGGCACATGAGGAGAATTATCCAGAATATTTAACGCCAGTCGCTggg                    >  2:352666/1‑248 (MQ=255)

                                                                                                                                                                                                                                attGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGGAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGGAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGa                    >  1:918683/1‑249 (MQ=255)

                                                                                                                                                                                                                                  ttttaaaaC‑GGGGG‑TTTGAT‑TTG‑TTGTCTC‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATa                  <  1:330860/244‑1 (MQ=255)

                                                                                                                                                                                                                                    ttaaaaC‑GGGCG‑TTTGAT‑TTT‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATAcc                <  1:186950/246‑1 (MQ=255)

                                                                                                                                                                                                                                        aaC‑TGGCG‑GTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCg           >  2:776696/1‑250 (MQ=255)

                                                                                                                                                                                                                                              gCG‑GTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAt      >  2:216000/1‑250 (MQ=255)

                                                                                                                                                                                                                                               gggtttGAT‑TTT‑TTTTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTaa        <  1:305172/244‑1 (MQ=255)

                                                                                                                                                                                                                                               cG‑GTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAtg     >  1:682855/1‑250 (MQ=255)

                                                                                                                                                                                                                                                   ttGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGT‑AAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAtgctg  >  1:570697/1‑249 (MQ=255)

                                                                                                                                                                                                                                                   ttGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGGAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAtgctg  >  2:626546/1‑250 (MQ=255)

GCATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTT‑ACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAT‑CAGCCG‑TTATATTTATTGTTTAAAC‑TGGCG‑GTTGAA‑TTG‑TTGTCTT‑C‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTT‑GCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGCTG  >  NZ_CP009273/2144922‑2145409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`