Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,036,3530CT50.0% 25.7 / 26.7 56F193F (TTC→TTT) srkAstress response kinase SrkA
Reads supporting (aligned to +/- strand):  ref base C (17/10);  new base T (27/0);  total (46/10)
Fisher's exact test for biased strand distribution p-value = 7.05e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCCAGTTCGAAGCTGATAATATCGATCAGATGGAAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTAT  >  NZ_CP009273/4036109‑4036602
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                          
gCCAGTTCGAAGCTGATAATATCGATCAGATGGAAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGt                                                                                                                                                                                                                                                       >  1:286485/1‑250 (MQ=255)
    gTTCGAAGCTGATAATATCGATCAGATGGAAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTg                                                                                                                                                                                                                                                   <  2:385080/250‑1 (MQ=255)
    gTTCGAAGCTGATAATATCGATCAGATGGAAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTTACCGTTCTg                                                                                                                                                                                                                                                   >  2:865342/1‑250 (MQ=255)
      tCGAAGCTGATAATATCGATCAGATGGAAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCg                                                                                                                                                                                                                                                 >  1:865589/1‑250 (MQ=255)
              gATAATATCGATCAGATGGAAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTTAACGTTTTGCGGcctcatt                                                                                                                                                                                                                                         >  1:781036/1‑244 (MQ=255)
                        aTCAGATGGAAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGGTTTGACCGGTCTGCGGGCACATTGAGACTGACa                                                                                                                                                                                                                               >  1:466032/1‑250 (MQ=255)
                        aTCAGATGGAAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCGACATGGAGAATGCCa                                                                                                                                                                                                                               >  2:695969/1‑250 (MQ=255)
                               ggAAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCggg                                                                                                                                                                                                                        >  1:593696/1‑250 (MQ=255)
                                 aaGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTGCCCGGTCTGCGGCTACATGGACACCGCCCCGCCgggg                                                                                                                                                                                                                       >  2:400154/1‑248 (MQ=255)
                                 aaGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGCGTGATTTTTTCGTTTTGCGGGTCCATTGGGTCTTGCCCGCCggggg                                                                                                                                                                                                                      >  2:791003/1‑248 (MQ=255)
                                    cGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGGTTTTACCGTTTTGCGGCTACATGGGGAATGCCCCGCCGGGAatat                                                                                                                                                                                                                   >  1:616751/1‑250 (MQ=255)
                                       ttGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGTGAGATTTCGTCCTTCTGCGTCGACATCGAGACCACCGCGCCGCGGATATtct                                                                                                                                                                                                                >  2:46959/1‑250 (MQ=255)
                                           gCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTCTCTgg                                                                                                                                                                                                            >  1:103404/1‑250 (MQ=255)
                                                                 cAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATTGGGACTGCCACGCCGGGAATATTCTCTTGCGCGGT‑GGTCCAATGTttgttg                                                                                                                                                                                     >  1:597306/1‑250 (MQ=255)
                                                                   gACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGGTACATGGAGAATGCCACGCCGGGGATATTTTCTTGCGCGGT‑GGGTCAATGTttgttgtt                                                                                                                                                                                   >  2:162405/1‑248 (MQ=255)
                                                                    aCGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCCACATGGAGACTGCCCCGCCGCGGAGATTTTCTGGCGCGCT‑GGTCCCAtgtttgtttttc                                                                                                                                                                                  >  2:699873/1‑246 (MQ=255)
                                                                           gcAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATAtt                                                                                                                                                                                                                  <  1:106947/212‑1 (MQ=255)
                                                                                 aGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCCGGGATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACg                                                                                                                                                                     >  1:96277/1‑250 (MQ=255)
                                                                                     ttttATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCCTTCTGGGGCTACATGGAGAATGCCCCGCCCGGGGTATTCTCTGGCGCGCT‑GGTGCAATGTTTGTTGTTCTGGATGATGCACGTAAt                                                                                                                                                                 >  2:292005/1‑250 (MQ=255)
                                                                                          tCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTTACCGTTCTGCGGCTACATGGGAACTGGCCCGCCGGGGATATTCTCTGGCGCGGT‑GGGCCAATTTTTTTTGGTCTGGGTGGTGCCCGGAATGGTc                                                                                                                                                             >  2:463850/1‑249 (MQ=255)
                                                                                          tCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCCCGCCGGGGATATTCTCTGGCGCGAT‑GGGCCAATGTTTTTTTATCTTGATGATGCACGTAATGGTc                                                                                                                                                             >  1:336272/1‑249 (MQ=255)
                                                                                           ccATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTTTCTGGCGCGGT‑GGTCCAATGTTTTTTGGTCTTGATGATGCACGTAATGGTc                                                                                                                                                             >  1:37727/1‑248 (MQ=255)
                                                                                            cATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTCACCCTTCTGCGGCCACAAGGAGACTGCCACGCCGGGGATATTCTCTTGCGCGGT‑GGGCCAATGTTGTTTGTTCTTGAAGGTGCACGTAATGGTTCAg                                                                                                                                                          >  2:431827/1‑250 (MQ=255)
                                                                                              tCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGcc                                                                                                                                                        >  1:25350/1‑250 (MQ=255)
                                                                                                gCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCCACATGGAGACTGGCCCGCCCGGGATATTCTCTGGCGCGGT‑TGTCCAATGTTTGTTTGTCTTGGTGATGGACGTAATgggtccgccgg                                                                                                                                                      >  2:459095/1‑241 (MQ=255)
                                                                                                  ccGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGCTc                                                                                                                                                    <  1:61222/250‑1 (MQ=255)
                                                                                                    gACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGGTTTGCGGCTACATGGAGACTGCCACCCCGGGGATATTCTCTGGCGCGGT‑GGGCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGGTCAg                                                                                                                                                  >  1:873538/1‑250 (MQ=255)
                                                                                                      ccATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGGTCTTCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGGTGATGCACGTAATGGGCCAGCCGTTCCGGa                                                                                                                                                >  1:355413/1‑250 (MQ=255)
                                                                                                           ggTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGCT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTtgt                                                                                                                                           >  1:340526/1‑250 (MQ=255)
                                                                                                                 aaCGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCAc                                                                                                                                                                                                                              >  1:631876/1‑162 (MQ=255)
                                                                                                                  aCGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACg                                                                                                                                                                                                                             <  2:631876/162‑1 (MQ=255)
                                                                                                                       tatCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTTGCGCGGT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGGAATGGTCCAGCCGTTCAGGATTTGTGGGTGTTGCTc                                                                                                                                >  1:190104/1‑249 (MQ=255)
                                                                                                                            cATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTCTCTGGCGCGCT‑GGTCCAATGTTTTTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGGTGTTGTTCAATggg                                                                                                                          >  1:454559/1‑249 (MQ=255)
                                                                                                                                   ccACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAAATTTTACCGTTCTGCGGCCACATGGGGACTGCCCCCCCGGGGATATTTTCTTGCGCGGT‑GGTTCCATGTTTGTTTGTTTGGGTGATGGACGTAATGGTTCAGCCGTTCCGGGTTTTTGGGGGGTGGTCAATTGGGGTaaa                                                                                                                    >  2:300165/1‑249 (MQ=255)
                                                                                                                                            cTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTCCCGTTCTGCGGGTCCATGGAGACTGCCACGCCCGGGATATTTTCTTGCGCGGT‑GGGCCAATGTTTGTTGATCTGGGTGATGCACGTAAAGGTTCAGCCGGTCTGGGTTTGTGGTTGTTGCGTAATGGCGATAAAAGCGagcaac                                                                                                          >  2:915491/1‑248 (MQ=255)
                                                                                                                                               tttGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCCTTCTGCGGCCACACGGGGACTGCCCCCCCGGGGATATTCTCTGGCGCGGT‑TGTCCAATGGTTTTTGGTCTGGATGATGCACGTAATGGTCCACCCGTTCCGGGTTTTTGGATGGTGCTCCATGGCGGTAAAGCCCAGGCGCggg                                                                                                       >  2:254162/1‑249 (MQ=255)
                                                                                                                                               tttGAGGACGCTACACTGATACCTCCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCgg                                                                                                        <  2:394042/249‑1 (MQ=255)
                                                                                                                                                                    cttcccGGGTGGAAAGCGGCATTCCTGAAGGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTg                                                                                  <  2:933732/246‑1 (MQ=255)
                                                                                                                                                                     cTTCCGGGTTGAAAGTGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTTTCTTGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGGTTTGTGGGTGTTTCTCAATTGCGATAAAACCGAGCAGAGGATGCAACTGGGAACTatttttt                                                                                  >  1:222851/1‑248 (MQ=255)
                                                                                                                                                                        ccGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGGT‑GGTCCCAATTTTTTTGATCTGGGGGATGCACGTAATGGTCCAGCCGTTCAGGGTTTGTGGGTGTTTCTCCATGGCGATGAAAGCGGGACGAGGGTGGGACTGGGAAATATTTTTGaaac                                                                              >  2:136211/1‑248 (MQ=255)
                                                                                                                                                                        ccGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTTTCTGGCGCGAT‑GGTCCCATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGGTCAGGATTTGTGGGTGGTGCTCAATGGCGATAAAACCGAGCAGCGGGTGCAACTGGGAACTATTATTGAAGc                                                                              >  1:552530/1‑250 (MQ=255)
                                                                                                                                                                            gTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCGACATGGAGACTGCCACGCCGGGGATATTCTCTGGCGCGAT‑GGTCCAATGTTTTTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCCGGATTTTTGGAGGTTGCTCAATGGCGGTAAAAACGAGCAGCGGGTGCAACTGGAAAATATTATTTAAACTTAt                                                                          >  2:412158/1‑250 (MQ=255)
                                                                                                                                                                             ttGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGAATTTACCGTTCTGCGGGCACATGGAGACTGCCACGCCGGGGATATTTTTTGGGGCGGTGGGTCCAA‑GTTTGTTTATCTGGGTGGTGCACGGAAAGGGCCAGCCGGTCCGGGTTTGTGGGTGTTGGTCAATGGCGGTAAAACCGGGGAGCGGGAGGCACTGGAAAATTTTTTTTAAGCTTTTg                                                                         >  2:443951/1‑250 (MQ=255)
                                                                                                                                                                               gAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATga                                                                        <  2:167912/249‑1 (MQ=255)
                                                                                                                                                                                        aTTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGc                                                              <  1:909349/250‑1 (MQ=255)
                                                                                                                                                                                         ttCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGGATATTTTCTGGCGCGGT‑GGTCCAATGTTTGTTGATCTGGGTGATGCACGTAATGGTCCAGCCGGTCAGGATTTTTGGATGTTGCTCAATGGCGGTAAAACCGAGCAGCGGGTGCAACTTGAAAATATTATTTAAGCTTATGAAGAATTTAGCg                                                             >  1:231858/1‑250 (MQ=255)
                                                                                                                                                                                                  gCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGGAGATTTTACCGTTCTGCTGCTACAAGGTGACCGCCACCACCGGGATATTCTTTTGCGCCAT‑GGTCCCATGTTTTTTGAACTGGGTTATTCACGTAAAGGTGCAGCCGTTTAGGGTTTGTGGATGTTGATCAATGGCGGTAAAGCCGAGCAGCCGGTGGAAATGGGAAAGATTATTGGAGGCTATGAAGAAGTTAGAGGGTTCggc                                                     >  2:390948/1‑247 (MQ=255)
                                                                                                                                                                                                    gACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGAATTTACCGTTCTGCGGCTCCATGGAGACTGCCCCGGCGGGGATATTTTCTGGCGCGGT‑GGTCCCATGTTTTTTTATCTGGGTGATGCACGTAATGGGCCAGCCGGTCCGGGTTTTTGGGTGTTGGTCCATGGCGATAAAGCCCGGCAGCGGGTGCAAATGGAAACTATTATTTAAGCTTATGAAAAATTTATAGGGTTCGaccac                                                  >  2:892750/1‑247 (MQ=255)
                                                                                                                                                                                                      cAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGGTGATGCACGTAATGGTCCAGCCGTTCAGGGTTTGTGGGTGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGc                                                >  1:476103/1‑250 (MQ=255)
                                                                                                                                                                                                         aTGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTTCGGCCACATGGAGACTGCCACGCCGGGGATATTCTCTGGCGCGGT‑GGGCCCATGTTTTTTGATCTGGATGATGCACGGAATGGGCCAGCCGTTCCGGGTTTGTGGGTGTTGCTCCATGGCGATAAAACCGAGCAGCGGGGGGAACTGGGAAATATTATTTAAGCTTTTGAAGAAATTAGCGGGTTCGACACCGccg                                              >  2:528790/1‑247 (MQ=255)
                                                                                                                                                                                                                    gccgccGTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCCACATGGGGAATGCCCCGCCGGGGATATTTTCTGGCGCGAT‑GGGTCCATGTTTGTTGATCTGGATGATGCACGCAATGGGCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGGGATAAAGCCGAGCAGCGGAGGGCACTGGGAACTATTTTTGAAGCTTTTGAAGAAATTTGCGGGTTCGACACCGCGGGAAACGGGCTgg                                  >  2:661553/1‑249 (MQ=255)
                                                                                                                                                                                                                         cgTTACAGCACACTGGCGGGAAGATTTTACCGTTCTGCGGCGACATGGAGACTGCCCCGCCGGGGATATTCTCTGGCGCGGT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGGTTTGTGGATGTTGTTCAATGGCGATAAAGCCGCGCAGCGGATGGAACTGGGAACTATTATTTAAGCTTATTAAGAATTTAGCGAGTTCGACAACCCTGAAATCGGACTGATTGaa                             >  2:360949/1‑250 (MQ=255)
                                                                                                                                                                                                                               aGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTa                       <  1:214751/250‑1 (MQ=255)
                                                                                                                                                                                                                                               gATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTgg       >  1:89167/1‑250 (MQ=255)
                                                                                                                                                                                                                                                  ttCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGtt     >  1:857183/1‑249 (MQ=255)
                                                                                                                                                                                                                                                   tCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGttt    <  1:76511/249‑1 (MQ=255)
                                                                                                                                                                                                                                                    cACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTttat  >  2:18911/1‑250 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                          
GCCAGTTCGAAGCTGATAATATCGATCAGATGGAAGCGGTTGGGCGTTATTTAGGGCGTATGCACCAGACGGGGCGCAAACAGCTTTTTATCCATCGCCCGACCATCGGTTTGAACGAATATCTCATTGAGCCACGCAAGCTGTTTGAGGACGCTACACTGATACCTTCCGGGTTGAAAGCGGCATTCCTGAAAGCGACAGATGAGCTGATTGCCGCCGTTACAGCACACTGGCGGGAAGATTTCACCGTTCTGCGGCTACATGGAGACTGCCACGCCGGGAATATTCTCTGGCGCGAT‑GGTCCAATGTTTGTTGATCTGGATGATGCACGTAATGGTCCAGCCGTTCAGGATTTGTGGATGTTGCTCAATGGCGATAAAGCCGAGCAGCGGATGCAACTGGAAACTATTATTGAAGCTTATGAAGAATTTAGCGAGTTCGACACCGCTGAAATCGGACTGATTGAACCTTTACGCGCCATGCGTTTGGTTTAT  >  NZ_CP009273/4036109‑4036602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑