Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,221,722	0	G	C	30.2%	91.3 / 11.6	54	pseudogene (516/1436 nt)	mdtQ	multidrug resistance outer membrane protein MdtQ
Reads supporting (aligned to +/- strand):  ref base G (15/22);  new base C (15/1);  total (30/24)
Fisher's exact test for biased strand distribution p-value = 2.75e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTTCATTTTCCCCGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCT‑CGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCTTTCCCCAGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAG  >  NZ_CP009273/2221474‑2221971                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                    tttcattttcCCCGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTTCCAGCCCGCCATTTGC‑GGGGTT‑GCTCCGCG‑TCCGCCCCCCgc                                                                                                                                                                                                                                                                    >  2:497231/1‑249 (MQ=255)   tcattttcCCCGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCT‑CGCGTTCCGCCGCCCGTGc                                                                                                                                                                                                                                                                  >  1:766030/1‑250 (MQ=255)       tttcCCCGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTTGC‑GGGTTT‑TCT‑CGCGTTCCGCCCCCCCTGCttt                                                                                                                                                                                                                                                               >  1:1491487/1‑249 (MQ=255)          ccccGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑TCT‑CGCGGTTCCCCGCCCCTGCTTTTA‑cc                                                                                                                                                                                                                                                          >  1:1062494/1‑250 (MQ=255)          ccccGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑GGT‑CCCTGTTCGCCCCCCCTGCCTTTA‑cc                                                                                                                                                                                                                                                          >  1:1038494/1‑250 (MQ=255)          ccccGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑GCT‑CGCGTTCCGCCGCCCGTGCTTTTA‑cc                                                                                                                                                                                                                                                          >  1:595173/1‑250 (MQ=255)           cccGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCT‑CGCGTTCCGCCGCCCGTGCTTTAA‑cc                                                                                                                                                                                                                                                          <  2:792948/249‑1 (MQ=255)                 aCATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCT‑CGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCa                                                                                                                                                                                                                                                  <  2:1098497/250‑1 (MQ=255)                      gTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCCGCAATTGGCGGGGTTT‑TTC‑CGCTTTCCCCCCCCCG‑GGTTTAA‑CCGCA‑CCCaagggg                                                                                                                                                                                                                                             >  1:57927/1‑245 (MQ=255)                      gTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCT‑CGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCgg                                                                                                                                                                                                                                             <  1:619068/250‑1 (MQ=255)                      gTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑TCC‑CCCGTTTCCCCCCCCCGGCTTTTA‑CCCTA‑ACCcggcgg                                                                                                                                                                                                                                             >  1:26890/1‑250 (MQ=255)                        tGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CCACCCTGCCACCCAGCAATTGGC‑GGGTTT‑GAT‑TCCGGTCCTCCGCCCCTGGTTTTA‑CCACA‑CTCAGGCgggg                                                                                                                                                                                                                                           >  2:689012/1‑249 (MQ=255)                         gAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑GCT‑CGCGGTTCCCCCCCCCCGCGTTTA‑CCGGA‑CCCCCGCGGGcg                                                                                                                                                                                                                                          >  2:924321/1‑249 (MQ=255)                         caGCTACTGCCGGCTTTTGACGGCATTAATATCGGCTTCCACAGCTTCAACTGAAGAATTAATCCCGTTCTGATATAACTGCGGATCGTTCAAGATAATGGTGTTCTGCGCTTTTTCGATGTGCTG‑CAAGACCGTGTTTAG‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GGT‑CGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGc                                                                                                                                                                                                                                           <  2:1531336/248‑1 (MQ=255)                            ctgctgCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCT‑CGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTa                                                                                                                                                                                                                                       <  1:590209/250‑1 (MQ=255)                               ctgcCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GGT‑CGCGGTCCCCCGCCCCGGCTTTTA‑ACGTA‑CCCAGGCggggcggaacc                                                                                                                                                                                                                                    >  1:1281921/1‑242 (MQ=255)                               ctgcCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCT‑CGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAAcc                                                                                                                                                                                                                                    <  1:238786/250‑1 (MQ=255)                               ctgcCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCT‑CGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAAcc                                                                                                                                                                                                                                    <  1:921872/250‑1 (MQ=255)                                            cTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACCCTTCCAACCAGCAATTGGC‑GGGGTT‑GGC‑CCCGTTCCGCCGCCCGCGCTTTCA‑CCGTA‑CCCAACCCGGCAGCAAACTccc‑cccccttcc                                                                                                                                                                                                                      >  1:159378/1‑244 (MQ=255)                                             tGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑GCT‑CGCGTTCCGCCGCCCCGGCTTTTA‑ACCTA‑ACCCGGCGGGCGGTAACCTCCC‑CCCGG‑TTCtt                                                                                                                                                                                                                    >  1:597713/1‑250 (MQ=255)                                              ggCATAAAAATCGGCTTCCACTCCGTCAACGAAAGAAGTAATCCCGTTGTGAGATTGCTGGCGATCGGTCGCGATAAGGGTGTTCGGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑GG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCT‑CGCGTGCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCtt                                                                                                                                                                                                                    <  2:887030/249‑1 (MQ=255)                                               gCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCT‑CGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCttt                                                                                                                                                                                                                   <  2:225947/249‑1 (MQ=255)                                               gCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCT‑CGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCTTTc                                                                                                                                                                                                                  <  2:766030/250‑1 (MQ=255)                                                      tatCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGCGGGGTTT‑GTC‑CGGGTT‑CGCCCCCCCCGCCTTTA‑CCCTG‑CCCAGGGGGGGCGTAACCCCCGCCCCGC‑TTTTTTcccccat                                                                                                                                                                                                            >  2:1407198/1‑247 (MQ=255)                                                        tCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTTCCCGCCAGCAATTGGC‑GGGGTT‑TCT‑CGCGCTCCGCCCCCCGCGCTCTTA‑CCCTA‑CCCCCGCGGGCAGCATACCCCC‑CCCCC‑CTCTTTCCCCCCatat                                                                                                                                                                                                         >  2:1269976/1‑250 (MQ=255)                                                             ttCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑TGT‑CGCGGTCCGCCGCCCCGGCTTTTA‑CCCTA‑ACCCGGCGGGCCGGAACCCCCC‑CCCGA‑TTCTTTCCCCCGATATc                                                                                                                                                                                                        >  1:289262/1‑246 (MQ=255)                                                                  cacCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTATTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCT‑CGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCTTTCCCCAGATATCGAGAT‑GCCa                                                                                                                                                                                              <  1:950089/250‑1 (MQ=255)                                                                        cAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCT‑CGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCTTTCCCCAGATATCGAGAT‑GCCAG‑ccc                                                                                                                                                                                         <  2:574965/248‑1 (MQ=255)                                                                        cAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑TCT‑CGCGGTCCGCCCCCCCTGCTTTTA‑CCCGA‑CCCAGGCGGGCCGTAAACCCCC‑CCCGC‑TTCTTTCCCCCGATATCGGGAT‑GCccg‑cccgc                                                                                                                                                                                       >  1:9675/1‑250 (MQ=255)                                                                        cAACTGAAGAAGTAATCCCGTTCTGATATAGCGGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCT‑CGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCTTTCCCCAGATATCGAGAT‑GCCAG‑ccc                                                                                                                                                                                         <  2:153170/248‑1 (MQ=255)                                                                                              cTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑GGT‑CGCGGTCCGCCCCCCCTGCTTTTA‑CCCTA‑CCCCGGCGGGCCGTAAACCCCG‑CCCGG‑TTTTTTTCCCCGATATCGGGAT‑TGCCG‑CCCGCCG‑TTAAGCCCAAAATAACC‑TTg                                                                                                                                                               >  1:642812/1‑250 (MQ=255)                                                                                                          gCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑TCT‑CGCGTTCCGCCGCCCGGGCTTTAA‑CCGTA‑CCCAGGCGGGCCGTAAACTCCG‑CCCGA‑TTCTTTTCCCCGATATCGAGAT‑GCCCA‑CCCGCCC‑GTAAGCCAAAAGCACCG‑TTGGGGTACCACagg                                                                                                                                                   >  1:438950/1‑250 (MQ=255)                                                                                                             gtcggtcggTATAATGGTGTTCGTCTTTTTTTCTATTGGCTG‑CAAGACCGTTTTAAC‑CGCCGCGGGGTTT‑TG‑CCCCCCCCAGACCG‑GGCGGG‑CCACGCTCCCGGCCAGCATTGGGG‑GGTTTT‑GCC‑CGCGTCCCGCCCCCCGTCCTTAAC‑CCGTC‑CCAGGGGGGGCGGAACCCCCCG‑CCCGA‑TTTTTCCCCCAGAAATCGAGAG‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCg                                                                                                                                                <  2:601068/249‑1 (MQ=255)                                                                                                                ggTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑GGT‑CGCGGTCCGCCCCCCCCGGTTTTA‑CCGTA‑ACCCAGCGGGCCGCAAACTCCC‑CCCCG‑TTCTTTCCCCCGCTATACAGAA‑GCCCGACCCCCAC‑TTAACCCAAAAAAACCT‑GTGG‑GGACCACGGGCCgggc                                                                                                                                             >  2:1209340/1‑248 (MQ=255)                                                                                                                  tgccgttAATGTTGTTCTGCTCTTTTCCT‑TTTGGTG‑GAAAACCGTGGTTAACCGCCG‑CTGGGTT‑GG‑AC‑CCCCCCGAAGAGGGCGGGTCTAGGCGGCCGGCCGGCAGTGGGG‑GGGTTT‑GCC‑CGCGTCCCGCCGCCCGTGCTTAAC‑CCTTA‑CCCAGGCGGGGAATACCCCCCG‑CCCGA‑TTCTTCCCCCAAAAATCGGGAT‑GCCAC‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑Gt                                                                                                                                          <  2:562387/244‑1 (MQ=255)                                                                                                                                 tCTGCTTTTTTTCT‑TTTGCCGACAACCCGGTTGTTAC‑CGCC‑CCTGGGTTGTC‑CCTCCCCCAGTACG‑GGCGGT‑CTCCGCTGCCACCCCGAAATTGGC‑GGGTTT‑GCC‑CGCTTCCCGCCGCCCGTTTTTTAC‑CCGTA‑CCCGGGCGGGCAGTACCCTCCC‑CCCGT‑TTCTTCCCCCATATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGAt                                                                                                                            <  2:1497607/249‑1 (MQ=255)                                                                                                                                                        ccaacacggttttAA‑CCCCCCCTGGGGT‑TGCCCCCCCCCAGTACG‑GGGGGT‑CTCCGCCCCCGGCCAGAATTGGGC‑GGTTTT‑GCC‑CGCCTCCCGCCCCCCGTTTTTTAC‑CCGTC‑CCCGGGCGGGCAGTACCCTCCC‑CCCGA‑TTCTTCCCCCAGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCAt                                                                                                     <  2:22885/240‑1 (MQ=255)                                                                                                                                                        cAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑TCG‑CGCGTTCCCCCCCCCCCGCGTTTA‑CCGCA‑CCCCAGCGGGCCGTAACCCCCC‑CCCCAGTTTTTTCCCCA‑ATAACGAGAT‑GCCCA‑CCCCCCC‑GTAAGCCAAAAGTATCGCTCGGTG‑ACCCCGGGCCGGGCGGTCCTTGCGGCGGGTCCGTTC‑GTGCAGAACGCCCCCAt                                                                                                     >  2:685144/1‑250 (MQ=255)                                                                                                                                                            acctgttttcagcgccgcCTGGGTT‑GC‑CCACCCCCAGTACG‑GGCGGC‑TTCCGCTGCCCGCCAGAAATGGGG‑GGGTTT‑CCT‑CCCGTCCCCCCCCCCGTGCTTTAA‑CCGCA‑CCCAGGCGGGCAGAACCCCCCG‑CCCGA‑TTCTTCCCCCAGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAg                                                                                                 <  2:759048/238‑1 (MQ=255)                                                                                                                                                             ccGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CCACGCCGCCAGCCAGCAAATGGC‑GGGGTT‑TCT‑TTCGGTCCCCCCCCCCCGCTTTAA‑CCCCACCCCAGCGGGGCGGAACCCCCCC‑CCCCA‑TTTTTTCCCCAGGAAT‑GCGAT‑GACAA‑CCCCCCGCTTAACCCAAAA‑AATCC‑CTGGGGTACCCCGGGCCGGTC‑CCACCCTCTTCCCGCGCGTTCCGAGCA‑ACCGGCCCCACTAAgac                                                                                               >  2:960047/1‑248 (MQ=255)                                                                                                                                                                                  ggttgcgcccccccccGTCCA‑GGGGGG‑CTCCGCGCCCCGCCGGCATTGGGC‑GGGTTT‑CCT‑CGCGTCCCGCCCCCGTTGCTTTAC‑CCCCC‑CCGGGGCGGGAAGTCCCCCCCC‑CCCTG‑TTCTTTCCCCAGATATGGAGGG‑GCCGG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACAtttttt                                                                              <  2:1507852/237‑1 (MQ=255)                                                                                                                                                                                         ccACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑TCT‑CGCGGTTCGCCGCCCGGGGTTTTA‑ACGTA‑ACCCGGGGGGGAGGAACCTCCC‑CCCCG‑ATCTTTCCCCCGATAACGGGATGGCCAG‑CCCCCGG‑TTAGCCAAAAAGAACGG‑TGGGGGTCCACCGGGCGGGGC‑GAACCCGC‑GCCGGGGCGGTTAGAGGACAACGGGCCCCTAAACCTTCCTGCGGCCAtttttgtcgcccc                                                                       >  2:499426/1‑242 (MQ=255)                                                                                                                                                                                                            gg‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑TCT‑CGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCCCCG‑CCCGC‑TTCTTTCCCCCGATATCGAGAT‑GCCAG‑ACCGCCC‑TTAAGCCCAAAATACCG‑TTGGTGTACCACGGGCCGGGC‑GGACCCACGGCCGGATCGGTCAGAGCA‑AACAGCGCCATTTAGCCCTCTGCCCGCATTTTTTGTCGCCCCATCTCCGCCGCAAAgac                                                     >  2:320890/1‑247 (MQ=255)                                                                                                                                                                                                                  cGCTGCCAGCCAGCAATTGGC‑GGGTTT‑TCT‑CGCGTTCCGCCGCCCGTGCTTTTA‑CCGTA‑CCCCGGCGGGCAGTAACCTCCG‑CCCGC‑TTCTTTCCCCAGATATCGAGAT‑GCCAG‑CCCCCCC‑TTAAGCCCAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGGTCGTTCCGAGCA‑AACGGCCCCCTTAAGCCTTCTGCCGCCATTTTTTTCCCCCCCCTCTCCCCCCAAAAGTCGATCtt                                               >  2:976221/1‑249 (MQ=255)                                                                                                                                                                                                                      gccagccaACAATTGGC‑GGGTTT‑GCT‑CGCGTCCCGCCGCCCGTGTTTTAC‑CCGCC‑CCCAGGCGGGAAGAACCCCCCG‑CCCGA‑TTCTTCCCCCAGATATCGAGAG‑GCCGG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGa                                           <  1:856397/250‑1 (MQ=255)                                                                                                                                                                                                                             agcAATGGGC‑GGTTTT‑GCG‑CGCGTCCCCCCGCCCGTGCTTTAC‑CCGTC‑CCCAGGCGGGCAGTACCCCCCG‑CCCAA‑TTCTTTCCCCAGATATCGAGAT‑CCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCt                                    <  1:75299/250‑1 (MQ=255)                                                                                                                                                                                                                               cccttGGC‑GGGTTTCGCC‑CGC‑TTCCCCCCCCCCTTTTTTAACCCGAC‑CCCGGGGGGGCAGT‑ACCCCCC‑CCCCT‑TTTTTTCCCCCAATTTCCGGGT‑GCCCGCCCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTg                                   <  2:289262/247‑1 (MQ=255)                                                                                                                                                                                                                                  ttGGC‑GGGTTT‑GCT‑CGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCTTTCCCCAGATATCGAGAT‑GCCCG‑CCCGCCG‑TTAAGCCAAAAATACCG‑GTTGTGTACCACCGGCCGGGC‑GTACCTGCGGGCGGAACGGTCAGAGCA‑AAAGGCCCCATTTAGCCTTCTGCCGACATTTTTTGCCGCTCCATATTCCCCGCAAAGTCGATCTGCGGACCATCCTGCGTg                               >  2:526205/1‑250 (MQ=255)                                                                                                                                                                                                                                       ggctgtttgccgcgcgTCCCGCCCCCCGCTTTTTAC‑CCGCC‑CCCGGGCGGGCAGTACCCCCCC‑CCCTT‑TTCTCTCCCCATATCTGGAGAT‑CCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTgg                              <  2:329817/237‑1 (MQ=255)                                                                                                                                                                                                                                          gTTT‑GCT‑CGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCTTTCCCCAGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTg                        >  1:735847/1‑250 (MQ=255)                                                                                                                                                                                                                                          gTTT‑GCT‑CGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCTTTCCCCAGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTg                        >  1:602011/1‑250 (MQ=255)                                                                                                                                                                                                                                                         ccgccgCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCTTTCCCCAGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGGCGATCTGCGGACCATCCTGAGTTGCAACTGCCTTCGCCTTGGc           >  1:1536632/1‑250 (MQ=255)                                                                                                                                                                                                                                                         ccgccgCCCGTCTTTTAC‑CCGTC‑CCCAGGCGGGAAGTACCCCCCC‑CCCGT‑TTCTTCCCCCAGATTCCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGc           <  1:362212/250‑1 (MQ=255)                                                                                                                                                                                                                                                            ccgccCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCTTTCCCCAGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCtt         >  1:32922/1‑249 (MQ=255)                                                                                                                                                                                                                                                               cccGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCTTTCCCCAGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCagc     >  1:930438/1‑250 (MQ=255)                                                                                                                                                                                                                                                                  gTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCTTTCCCCAGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCagctag  >  2:1401924/1‑250 (MQ=255)                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                    TTTCATTTTCCCCGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCT‑CGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCTTTCCCCAGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAG  >  NZ_CP009273/2221474‑2221971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑