Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,221,729	0	A	T	32.1%	79.6 / 12.7	54	pseudogene (509/1436 nt)	mdtQ	multidrug resistance outer membrane protein MdtQ
Reads supporting (aligned to +/- strand):  ref base A (11/25);  new base T (17/0);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 6.98e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.54e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CCCCGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCTTTCCCCAGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATG  >  NZ_CP009273/2221483‑2221978                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                    ccccGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑TCTCGCGGTTCCCCGCCCCTGCTTTTA‑cc                                                                                                                                                                                                                                                                 >  1:1062494/1‑250 (MQ=255) ccccGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑GGTCCCTGTTCGCCCCCCCTGCCTTTA‑cc                                                                                                                                                                                                                                                                 >  1:1038494/1‑250 (MQ=255) ccccGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑GCTCGCGTTCCGCCGCCCGTGCTTTTA‑cc                                                                                                                                                                                                                                                                 >  1:595173/1‑250 (MQ=255)  cccGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCTCGCGTTCCGCCGCCCGTGCTTTAA‑cc                                                                                                                                                                                                                                                                 <  2:792948/249‑1 (MQ=255)        aCATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCa                                                                                                                                                                                                                                                         <  2:1098497/250‑1 (MQ=255)             gTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCCGCAATTGGCGGGGTTT‑TTCCGCTTTCCCCCCCCCG‑GGTTTAA‑CCGCA‑CCCaagggg                                                                                                                                                                                                                                                    >  1:57927/1‑245 (MQ=255)             gTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCgg                                                                                                                                                                                                                                                    <  1:619068/250‑1 (MQ=255)             gTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑TCCCCCGTTTCCCCCCCCCGGCTTTTA‑CCCTA‑ACCcggcgg                                                                                                                                                                                                                                                    >  1:26890/1‑250 (MQ=255)               tGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CCACCCTGCCACCCAGCAATTGGC‑GGGTTT‑GATTCCGGTCCTCCGCCCCTGGTTTTA‑CCACA‑CTCAGGCgggg                                                                                                                                                                                                                                                  >  2:689012/1‑249 (MQ=255)                gAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑GCTCGCGGTTCCCCCCCCCCGCGTTTA‑CCGGA‑CCCCCGCGGGcg                                                                                                                                                                                                                                                 >  2:924321/1‑249 (MQ=255)                caGCTACTGCCGGCTTTTGACGGCATTAATATCGGCTTCCACAGCTTCAACTGAAGAATTAATCCCGTTCTGATATAACTGCGGATCGTTCAAGATAATGGTGTTCTGCGCTTTTTCGATGTGCTG‑CAAGACCGTGTTTAG‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GGTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGc                                                                                                                                                                                                                                                  <  2:1531336/248‑1 (MQ=255)                   ctgctgCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTa                                                                                                                                                                                                                                              <  1:590209/250‑1 (MQ=255)                      ctgcCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GGTCGCGGTCCCCCGCCCCGGCTTTTA‑ACGTA‑CCCAGGCggggcggaacc                                                                                                                                                                                                                                           >  1:1281921/1‑242 (MQ=255)                      ctgcCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAAcc                                                                                                                                                                                                                                           <  1:238786/250‑1 (MQ=255)                      ctgcCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAAcc                                                                                                                                                                                                                                           <  1:921872/250‑1 (MQ=255)                                   cTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACCCTTCCAACCAGCAATTGGC‑GGGGTT‑GGCCCCGTTCCGCCGCCCGCGCTTTCA‑CCGTA‑CCCAACCCGGCAGCAAACTccc‑cccccttcc                                                                                                                                                                                                                             >  1:159378/1‑244 (MQ=255)                                    tGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑GCTCGCGTTCCGCCGCCCCGGCTTTTA‑ACCTA‑ACCCGGCGGGCGGTAACCTCCC‑CCCGG‑TTCtt                                                                                                                                                                                                                           >  1:597713/1‑250 (MQ=255)                                     ggCATAAAAATCGGCTTCCACTCCGTCAACGAAAGAAGTAATCCCGTTGTGAGATTGCTGGCGATCGGTCGCGATAAGGGTGTTCGGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑GG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCTCGCGTGCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCtt                                                                                                                                                                                                                           <  2:887030/249‑1 (MQ=255)                                      gCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCttt                                                                                                                                                                                                                          <  2:225947/249‑1 (MQ=255)                                      gCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCTTTc                                                                                                                                                                                                                         <  2:766030/250‑1 (MQ=255)                                             tatCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGCGGGGTTT‑GTCCGGGTT‑CGCCCCCCCCGCCTTTA‑CCCTG‑CCCAGGGGGGGCGTAACCCCCGCCCCGC‑TTTTTTcccccat                                                                                                                                                                                                                   >  2:1407198/1‑247 (MQ=255)                                               tCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTTCCCGCCAGCAATTGGC‑GGGGTT‑TCTCGCGCTCCGCCCCCCGCGCTCTTA‑CCCTA‑CCCCCGCGGGCAGCATACCCCC‑CCCCC‑CTCTTTCCCCCCatat                                                                                                                                                                                                                >  2:1269976/1‑250 (MQ=255)                                                    ttCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑TGTCGCGGTCCGCCGCCCCGGCTTTTA‑CCCTA‑ACCCGGCGGGCCGGAACCCCCC‑CCCGA‑TTCTTTCCCCCGATATc                                                                                                                                                                                                               >  1:289262/1‑246 (MQ=255)                                                         cacCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTATTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCTTTCCCCAGATATCGAGAT‑GCCa                                                                                                                                                                                                     <  1:950089/250‑1 (MQ=255)                                                               cAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCTTTCCCCAGATATCGAGAT‑GCCAG‑ccc                                                                                                                                                                                                <  2:574965/248‑1 (MQ=255)                                                               cAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑TCTCGCGGTCCGCCCCCCCTGCTTTTA‑CCCGA‑CCCAGGCGGGCCGTAAACCCCC‑CCCGC‑TTCTTTCCCCCGATATCGGGAT‑GCccg‑cccgc                                                                                                                                                                                              >  1:9675/1‑250 (MQ=255)                                                               cAACTGAAGAAGTAATCCCGTTCTGATATAGCGGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCTTTCCCCAGATATCGAGAT‑GCCAG‑ccc                                                                                                                                                                                                <  2:153170/248‑1 (MQ=255)                                                                                     cTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑GGTCGCGGTCCGCCCCCCCTGCTTTTA‑CCCTA‑CCCCGGCGGGCCGTAAACCCCG‑CCCGG‑TTTTTTTCCCCGATATCGGGAT‑TGCCG‑CCCGCCG‑TTAAGCCCAAAATAACC‑TTg                                                                                                                                                                      >  1:642812/1‑250 (MQ=255)                                                                                                 gCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑TCTCGCGTTCCGCCGCCCGGGCTTTAA‑CCGTA‑CCCAGGCGGGCCGTAAACTCCG‑CCCGA‑TTCTTTTCCCCGATATCGAGAT‑GCCCA‑CCCGCCC‑GTAAGCCAAAAGCACCG‑TTGGGGTACCACagg                                                                                                                                                          >  1:438950/1‑250 (MQ=255)                                                                                                    gtcggtcggTATAATGGTGTTCGTCTTTTTTTCTATTGGCTG‑CAAGACCGTTTTAAC‑CGCCGCGGGGTTT‑TG‑CCCCCCCCAGACCG‑GGCGGG‑CCACGCTCCCGGCCAGCATTGGGG‑GGTTTT‑GCCCGCGTCCCGCCCCCCGTCCTTAAC‑CCGTC‑CCAGGGGGGGCGGAACCCCCCG‑CCCGA‑TTTTTCCCCCAGAAATCGAGAG‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCg                                                                                                                                                       <  2:601068/249‑1 (MQ=255)                                                                                                       ggTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑GGTCGCGGTCCGCCCCCCCCGGTTTTA‑CCGTA‑ACCCAGCGGGCCGCAAACTCCC‑CCCCG‑TTCTTTCCCCCGCTATACAGAA‑GCCCGACCCCCAC‑TTAACCCAAAAAAACCT‑GTGG‑GGACCACGGGCCgggc                                                                                                                                                    >  2:1209340/1‑248 (MQ=255)                                                                                                         tgccgttAATGTTGTTCTGCTCTTTTCCT‑TTTGGTG‑GAAAACCGTGGTTAACCGCCG‑CTGGGTT‑GG‑AC‑CCCCCCGAAGAGGGCGGGTCTAGGCGGCCGGCCGGCAGTGGGG‑GGGTTT‑GCCCGCGTCCCGCCGCCCGTGCTTAAC‑CCTTA‑CCCAGGCGGGGAATACCCCCCG‑CCCGA‑TTCTTCCCCCAAAAATCGGGAT‑GCCAC‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑Gt                                                                                                                                                 <  2:562387/244‑1 (MQ=255)                                                                                                                        tCTGCTTTTTTTCT‑TTTGCCGACAACCCGGTTGTTAC‑CGCC‑CCTGGGTTGTC‑CCTCCCCCAGTACG‑GGCGGT‑CTCCGCTGCCACCCCGAAATTGGC‑GGGTTT‑GCCCGCTTCCCGCCGCCCGTTTTTTAC‑CCGTA‑CCCGGGCGGGCAGTACCCTCCC‑CCCGT‑TTCTTCCCCCATATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGAt                                                                                                                                   <  2:1497607/249‑1 (MQ=255)                                                                                                                                               ccaacacggttttAA‑CCCCCCCTGGGGT‑TGCCCCCCCCCAGTACG‑GGGGGT‑CTCCGCCCCCGGCCAGAATTGGGC‑GGTTTT‑GCCCGCCTCCCGCCCCCCGTTTTTTAC‑CCGTC‑CCCGGGCGGGCAGTACCCTCCC‑CCCGA‑TTCTTCCCCCAGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCAt                                                                                                            <  2:22885/240‑1 (MQ=255)                                                                                                                                               cAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑TCGCGCGTTCCCCCCCCCCCGCGTTTA‑CCGCA‑CCCCAGCGGGCCGTAACCCCCC‑CCCCAGTTTTTTCCCCA‑ATAACGAGAT‑GCCCA‑CCCCCCC‑GTAAGCCAAAAGTATCGCTCGGTG‑ACCCCGGGCCGGGCGGTCCTTGCGGCGGGTCCGTTC‑GTGCAGAACGCCCCCAt                                                                                                            >  2:685144/1‑250 (MQ=255)                                                                                                                                                   acctgttttcagcgccgcCTGGGTT‑GC‑CCACCCCCAGTACG‑GGCGGC‑TTCCGCTGCCCGCCAGAAATGGGG‑GGGTTT‑CCTCCCGTCCCCCCCCCCGTGCTTTAA‑CCGCA‑CCCAGGCGGGCAGAACCCCCCG‑CCCGA‑TTCTTCCCCCAGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAg                                                                                                        <  2:759048/238‑1 (MQ=255)                                                                                                                                                    ccGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CCACGCCGCCAGCCAGCAAATGGC‑GGGGTT‑TCTTTCGGTCCCCCCCCCCCGCTTTAA‑CCCCACCCCAGCGGGGCGGAACCCCCCC‑CCCCA‑TTTTTTCCCCAGGAAT‑GCGAT‑GACAA‑CCCCCCGCTTAACCCAAAA‑AATCC‑CTGGGGTACCCCGGGCCGGTC‑CCACCCTCTTCCCGCGCGTTCCGAGCA‑ACCGGCCCCACTAAgac                                                                                                      >  2:960047/1‑248 (MQ=255)                                                                                                                                                                         ggttgcgcccccccccGTCCA‑GGGGGG‑CTCCGCGCCCCGCCGGCATTGGGC‑GGGTTT‑CCTCGCGTCCCGCCCCCGTTGCTTTAC‑CCCCC‑CCGGGGCGGGAAGTCCCCCCCC‑CCCTG‑TTCTTTCCCCAGATATGGAGGG‑GCCGG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACAtttttt                                                                                     <  2:1507852/237‑1 (MQ=255)                                                                                                                                                                                ccACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑TCTCGCGGTTCGCCGCCCGGGGTTTTA‑ACGTA‑ACCCGGGGGGGAGGAACCTCCC‑CCCCG‑ATCTTTCCCCCGATAACGGGATGGCCAG‑CCCCCGG‑TTAGCCAAAAAGAACGG‑TGGGGGTCCACCGGGCGGGGC‑GAACCCGC‑GCCGGGGCGGTTAGAGGACAACGGGCCCCTAAACCTTCCTGCGGCCAtttttgtcgcccc                                                                              >  2:499426/1‑242 (MQ=255)                                                                                                                                                                                                   gg‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑TCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCCCCG‑CCCGC‑TTCTTTCCCCCGATATCGAGAT‑GCCAG‑ACCGCCC‑TTAAGCCCAAAATACCG‑TTGGTGTACCACGGGCCGGGC‑GGACCCACGGCCGGATCGGTCAGAGCA‑AACAGCGCCATTTAGCCCTCTGCCCGCATTTTTTGTCGCCCCATCTCCGCCGCAAAgac                                                            >  2:320890/1‑247 (MQ=255)                                                                                                                                                                                                         cGCTGCCAGCCAGCAATTGGC‑GGGTTT‑TCTCGCGTTCCGCCGCCCGTGCTTTTA‑CCGTA‑CCCCGGCGGGCAGTAACCTCCG‑CCCGC‑TTCTTTCCCCAGATATCGAGAT‑GCCAG‑CCCCCCC‑TTAAGCCCAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGGTCGTTCCGAGCA‑AACGGCCCCCTTAAGCCTTCTGCCGCCATTTTTTTCCCCCCCCTCTCCCCCCAAAAGTCGATCtt                                                      >  2:976221/1‑249 (MQ=255)                                                                                                                                                                                                             gccagccaACAATTGGC‑GGGTTT‑GCTCGCGTCCCGCCGCCCGTGTTTTAC‑CCGCC‑CCCAGGCGGGAAGAACCCCCCG‑CCCGA‑TTCTTCCCCCAGATATCGAGAG‑GCCGG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGa                                                  <  1:856397/250‑1 (MQ=255)                                                                                                                                                                                                                    agcAATGGGC‑GGTTTT‑GCGCGCGTCCCCCCGCCCGTGCTTTAC‑CCGTC‑CCCAGGCGGGCAGTACCCCCCG‑CCCAA‑TTCTTTCCCCAGATATCGAGAT‑CCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCt                                           <  1:75299/250‑1 (MQ=255)                                                                                                                                                                                                                      cccttGGC‑GGGTTTCGCCCGC‑TTCCCCCCCCCCTTTTTTAACCCGAC‑CCCGGGGGGGCAGT‑ACCCCCC‑CCCCT‑TTTTTTCCCCCAATTTCCGGGT‑GCCCGCCCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTg                                          <  2:289262/247‑1 (MQ=255)                                                                                                                                                                                                                         ttGGC‑GGGTTT‑GCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCTTTCCCCAGATATCGAGAT‑GCCCG‑CCCGCCG‑TTAAGCCAAAAATACCG‑GTTGTGTACCACCGGCCGGGC‑GTACCTGCGGGCGGAACGGTCAGAGCA‑AAAGGCCCCATTTAGCCTTCTGCCGACATTTTTTGCCGCTCCATATTCCCCGCAAAGTCGATCTGCGGACCATCCTGCGTg                                      >  2:526205/1‑250 (MQ=255)                                                                                                                                                                                                                             ggctgtttgccgcgcgTCCCGCCCCCCGCTTTTTAC‑CCGCC‑CCCGGGCGGGCAGTACCCCCCC‑CCCTT‑TTCTCTCCCCATATCTGGAGAT‑CCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTgg                                     <  2:329817/237‑1 (MQ=255)                                                                                                                                                                                                                                 gTTT‑GCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCTTTCCCCAGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTg                               >  1:735847/1‑250 (MQ=255)                                                                                                                                                                                                                                 gTTT‑GCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCTTTCCCCAGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTg                               >  1:602011/1‑250 (MQ=255)                                                                                                                                                                                                                                               ccgccgCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCTTTCCCCAGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGGCGATCTGCGGACCATCCTGAGTTGCAACTGCCTTCGCCTTGGc                  >  1:1536632/1‑250 (MQ=255)                                                                                                                                                                                                                                               ccgccgCCCGTCTTTTAC‑CCGTC‑CCCAGGCGGGAAGTACCCCCCC‑CCCGT‑TTCTTCCCCCAGATTCCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGc                  <  1:362212/250‑1 (MQ=255)                                                                                                                                                                                                                                                  ccgccCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCTTTCCCCAGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCtt                >  1:32922/1‑249 (MQ=255)                                                                                                                                                                                                                                                     cccGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCTTTCCCCAGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCagc            >  1:930438/1‑250 (MQ=255)                                                                                                                                                                                                                                                        gTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCTTTCCCCAGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCagctag         >  2:1401924/1‑250 (MQ=255)                                                                                                                                                                                                                                                             ttAC‑CCGTC‑CCCAGGCGGGCAGTACCCCCCC‑CCCGA‑TTCTTCCCCCAAATAGCGAGAC‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGa     <  1:1025046/249‑1 (MQ=255)                                                                                                                                                                                                                                                               aa‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCTTTCCCCAGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATg  >  1:1176599/1‑250 (MQ=255)                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                    CCCCGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAACCTCCG‑CCCGA‑TTCTTTCCCCAGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATG  >  NZ_CP009273/2221483‑2221978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑