Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,221,754	0	T	C	43.9%	57.9 / 18.1	57	pseudogene (484/1436 nt)	mdtQ	multidrug resistance outer membrane protein MdtQ
Reads supporting (aligned to +/- strand):  ref base T (22/10);  new base C (8/17);  total (30/27)
Fisher's exact test for biased strand distribution p-value = 7.98e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAA‑CCTCCG‑CCCGA‑TTCTTTCCCC‑AGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATC  >  NZ_CP009273/2221517‑2222001                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                 cTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACCCTTCCAACCAGCAATTGGC‑GGGGTT‑GGCCCCGTTCCGCCGCCCGCGCTTTCA‑CCGTA‑CCCAACCCGGCAGCAA‑ACTccc‑cccccttcc                                                                                                                                                                                                                                                     >  1:159378/1‑244 (MQ=255)  tGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑GCTCGCGTTCCGCCGCCCCGGCTTTTA‑ACCTA‑ACCCGGCGGGCGGTAA‑CCTCCC‑CCCGG‑TTCtt                                                                                                                                                                                                                                                   >  1:597713/1‑250 (MQ=255)   ggCATAAAAATCGGCTTCCACTCCGTCAACGAAAGAAGTAATCCCGTTGTGAGATTGCTGGCGATCGGTCGCGATAAGGGTGTTCGGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑GG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCTCGCGTGCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAA‑CCTCCG‑CCCGA‑TTCtt                                                                                                                                                                                                                                                   <  2:887030/249‑1 (MQ=255)    gCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAA‑CCTCCG‑CCCGA‑TTCttt                                                                                                                                                                                                                                                  <  2:225947/249‑1 (MQ=255)    gCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAA‑CCTCCG‑CCCGA‑TTCTTTc                                                                                                                                                                                                                                                 <  2:766030/250‑1 (MQ=255)           tatCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGCGGGGTTT‑GTCCGGGTT‑CGCCCCCCCCGCCTTTA‑CCCTG‑CCCAGGGGGGGCGTAA‑CCCCCGCCCCGC‑TTTTTTcccccat                                                                                                                                                                                                                                           >  2:1407198/1‑247 (MQ=255)             tCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTTCCCGCCAGCAATTGGC‑GGGGTT‑TCTCGCGCTCCGCCCCCCGCGCTCTTA‑CCCTA‑CCCCCGCGGGCAGCAT‑ACCCCC‑CCCCC‑CTCTTTCCCC‑CCatat                                                                                                                                                                                                                                       >  2:1269976/1‑250 (MQ=255)                  ttCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑TGTCGCGGTCCGCCGCCCCGGCTTTTA‑CCCTA‑ACCCGGCGGGCCGGAA‑CCCCCC‑CCCGA‑TTCTTTCCCC‑CGATATc                                                                                                                                                                                                                                      >  1:289262/1‑246 (MQ=255)                       cacCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTATTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAA‑CCTCCG‑CCCGA‑TTCTTTCCCC‑AGATATCGAGAT‑GCCa                                                                                                                                                                                                                            <  1:950089/250‑1 (MQ=255)                             cAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAA‑CCTCCG‑CCCGA‑TTCTTTCCCC‑AGATATCGAGAT‑GCCAG‑ccc                                                                                                                                                                                                                       <  2:574965/248‑1 (MQ=255)                             cAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑TCTCGCGGTCCGCCCCCCCTGCTTTTA‑CCCGA‑CCCAGGCGGGCCGTAA‑ACCCCC‑CCCGC‑TTCTTTCCCC‑CGATATCGGGAT‑GCccg‑cccgc                                                                                                                                                                                                                     >  1:9675/1‑250 (MQ=255)                             cAACTGAAGAAGTAATCCCGTTCTGATATAGCGGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAA‑CCTCCG‑CCCGA‑TTCTTTCCCC‑AGATATCGAGAT‑GCCAG‑ccc                                                                                                                                                                                                                       <  2:153170/248‑1 (MQ=255)                                                   cTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑GGTCGCGGTCCGCCCCCCCTGCTTTTA‑CCCTA‑CCCCGGCGGGCCGTAA‑ACCCCG‑CCCGG‑TTTTTTTCCC‑CGATATCGGGAT‑TGCCG‑CCCGCCG‑TTAAGCCCAAAATAACC‑TTg                                                                                                                                                                                             >  1:642812/1‑250 (MQ=255)                                                               gCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑TCTCGCGTTCCGCCGCCCGGGCTTTAA‑CCGTA‑CCCAGGCGGGCCGTAA‑ACTCCG‑CCCGA‑TTCTTTTCCC‑CGATATCGAGAT‑GCCCA‑CCCGCCC‑GTAAGCCAAAAGCACCG‑TTGGGGTACCACagg                                                                                                                                                                                 >  1:438950/1‑250 (MQ=255)                                                                  gtcggtcggTATAATGGTGTTCGTCTTTTTTTCTATTGGCTG‑CAAGACCGTTTTAAC‑CGCCGCGGGGTTT‑TG‑CCCCCCCCAGACCG‑GGCGGG‑CCACGCTCCCGGCCAGCATTGGGG‑GGTTTT‑GCCCGCGTCCCGCCCCCCGTCCTTAAC‑CCGTC‑CCAGGGGGGGCGGAAC‑CCCCCG‑CCCGA‑TTTTTCCCCC‑AGAAATCGAGAG‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCg                                                                                                                                                                              <  2:601068/249‑1 (MQ=255)                                                                     ggTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑GGTCGCGGTCCGCCCCCCCCGGTTTTA‑CCGTA‑ACCCAGCGGGCCGCAA‑ACTCCC‑CCCCG‑TTCTTTCCCC‑CGCTATACAGAA‑GCCCGACCCCCAC‑TTAACCCAAAAAAACCT‑GTGG‑GGACCACGGGCCgggc                                                                                                                                                                           >  2:1209340/1‑248 (MQ=255)                                                                       tgccgttAATGTTGTTCTGCTCTTTTCCT‑TTTGGTG‑GAAAACCGTGGTTAACCGCCG‑CTGGGTT‑GG‑AC‑CCCCCCGAAGAGGGCGGGTCTAGGCGGCCGGCCGGCAGTGGGG‑GGGTTT‑GCCCGCGTCCCGCCGCCCGTGCTTAAC‑CCTTA‑CCCAGGCGGGGAATAC‑CCCCCG‑CCCGA‑TTCTTCCCCC‑AAAAATCGGGAT‑GCCAC‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑Gt                                                                                                                                                                        <  2:562387/244‑1 (MQ=255)                                                                                      tCTGCTTTTTTTCT‑TTTGCCGACAACCCGGTTGTTAC‑CGCC‑CCTGGGTTGTC‑CCTCCCCCAGTACG‑GGCGGT‑CTCCGCTGCCACCCCGAAATTGGC‑GGGTTT‑GCCCGCTTCCCGCCGCCCGTTTTTTAC‑CCGTA‑CCCGGGCGGGCAGTAC‑CCTCCC‑CCCGT‑TTCTTCCCCC‑ATATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGAt                                                                                                                                                          <  2:1497607/249‑1 (MQ=255)                                                                                                             ccaacacggttttAA‑CCCCCCCTGGGGT‑TGCCCCCCCCCAGTACG‑GGGGGT‑CTCCGCCCCCGGCCAGAATTGGGC‑GGTTTT‑GCCCGCCTCCCGCCCCCCGTTTTTTAC‑CCGTC‑CCCGGGCGGGCAGTAC‑CCTCCC‑CCCGA‑TTCTTCCCCC‑AGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCAt                                                                                                                                   <  2:22885/240‑1 (MQ=255)                                                                                                             cAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑TCGCGCGTTCCCCCCCCCCCGCGTTTA‑CCGCA‑CCCCAGCGGGCCGTAA‑CCCCCC‑CCCCAGTTTTTTCCCC‑A‑ATAACGAGAT‑GCCCA‑CCCCCCC‑GTAAGCCAAAAGTATCGCTCGGTG‑ACCCCGGGCCGGGCGGTCCTTGCGGCGGGTCCGTTC‑GTGCAGAACGCCCCCAt                                                                                                                                   >  2:685144/1‑250 (MQ=255)                                                                                                                 acctgttttcagcgccgcCTGGGTT‑GC‑CCACCCCCAGTACG‑GGCGGC‑TTCCGCTGCCCGCCAGAAATGGGG‑GGGTTT‑CCTCCCGTCCCCCCCCCCGTGCTTTAA‑CCGCA‑CCCAGGCGGGCAGAAC‑CCCCCG‑CCCGA‑TTCTTCCCCC‑AGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAg                                                                                                                               <  2:759048/238‑1 (MQ=255)                                                                                                                  ccGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CCACGCCGCCAGCCAGCAAATGGC‑GGGGTT‑TCTTTCGGTCCCCCCCCCCCGCTTTAA‑CCCCACCCCAGCGGGGCGGAAC‑CCCCCC‑CCCCA‑TTTTTTCCCC‑AGGAAT‑GCGAT‑GACAA‑CCCCCCGCTTAACCCAAAA‑AATCC‑CTGGGGTACCCCGGGCCGGTC‑CCACCCTCTTCCCGCGCGTTCCGAGCA‑ACCGGCCCCACTAAgac                                                                                                                             >  2:960047/1‑248 (MQ=255)                                                                                                                                       ggttgcgcccccccccGTCCA‑GGGGGG‑CTCCGCGCCCCGCCGGCATTGGGC‑GGGTTT‑CCTCGCGTCCCGCCCCCGTTGCTTTAC‑CCCCC‑CCGGGGCGGGAAGTCC‑CCCCCC‑CCCTG‑TTCTTTCCCC‑AGATATGGAGGG‑GCCGG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACAtttttt                                                                                                            <  2:1507852/237‑1 (MQ=255)                                                                                                                                              ccACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGGTT‑TCTCGCGGTTCGCCGCCCGGGGTTTTA‑ACGTA‑ACCCGGGGGGGAGGAA‑CCTCCC‑CCCCG‑ATCTTTCCCC‑CGATAACGGGATGGCCAG‑CCCCCGG‑TTAGCCAAAAAGAACGG‑TGGGGGTCCACCGGGCGGGGC‑GAACCCGC‑GCCGGGGCGGTTAGAGGACAACGGGCCCCTAAACCTTCCTGCGGCCAtttttgtcgcccc                                                                                                     >  2:499426/1‑242 (MQ=255)                                                                                                                                                                 gg‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑TCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAA‑CCCCCG‑CCCGC‑TTCTTTCCCC‑CGATATCGAGAT‑GCCAG‑ACCGCCC‑TTAAGCCCAAAATACCG‑TTGGTGTACCACGGGCCGGGC‑GGACCCACGGCCGGATCGGTCAGAGCA‑AACAGCGCCATTTAGCCCTCTGCCCGCATTTTTTGTCGCCCCATCTCCGCCGCAAAgac                                                                                   >  2:320890/1‑247 (MQ=255)                                                                                                                                                                       cGCTGCCAGCCAGCAATTGGC‑GGGTTT‑TCTCGCGTTCCGCCGCCCGTGCTTTTA‑CCGTA‑CCCCGGCGGGCAGTAA‑CCTCCG‑CCCGC‑TTCTTTCCCC‑AGATATCGAGAT‑GCCAG‑CCCCCCC‑TTAAGCCCAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGGTCGTTCCGAGCA‑AACGGCCCCCTTAAGCCTTCTGCCGCCATTTTTTTCCCCCCCCTCTCCCCCCAAAAGTCGATCtt                                                                             >  2:976221/1‑249 (MQ=255)                                                                                                                                                                           gccagccaACAATTGGC‑GGGTTT‑GCTCGCGTCCCGCCGCCCGTGTTTTAC‑CCGCC‑CCCAGGCGGGAAGAAC‑CCCCCG‑CCCGA‑TTCTTCCCCC‑AGATATCGAGAG‑GCCGG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGa                                                                         <  1:856397/250‑1 (MQ=255)                                                                                                                                                                                  agcAATGGGC‑GGTTTT‑GCGCGCGTCCCCCCGCCCGTGCTTTAC‑CCGTC‑CCCAGGCGGGCAGTAC‑CCCCCG‑CCCAA‑TTCTTTCCCC‑AGATATCGAGAT‑CCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCt                                                                  <  1:75299/250‑1 (MQ=255)                                                                                                                                                                                    cccttGGC‑GGGTTTCGCCCGC‑TTCCCCCCCCCCTTTTTTAACCCGAC‑CCCGGGGGGGCAGT‑A‑CCCCCC‑CCCCT‑TTTTTTCCCC‑CAATTTCCGGGT‑GCCCGCCCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTg                                                                 <  2:289262/247‑1 (MQ=255)                                                                                                                                                                                       ttGGC‑GGGTTT‑GCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAA‑CCTCCG‑CCCGA‑TTCTTTCCCC‑AGATATCGAGAT‑GCCCG‑CCCGCCG‑TTAAGCCAAAAATACCG‑GTTGTGTACCACCGGCCGGGC‑GTACCTGCGGGCGGAACGGTCAGAGCA‑AAAGGCCCCATTTAGCCTTCTGCCGACATTTTTTGCCGCTCCATATTCCCCGCAAAGTCGATCTGCGGACCATCCTGCGTg                                                             >  2:526205/1‑250 (MQ=255)                                                                                                                                                                                           ggctgtttgccgcgcgTCCCGCCCCCCGCTTTTTAC‑CCGCC‑CCCGGGCGGGCAGTAC‑CCCCCC‑CCCTT‑TTCTCTCCCC‑ATATCTGGAGAT‑CCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTgg                                                            <  2:329817/237‑1 (MQ=255)                                                                                                                                                                                               gTTT‑GCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAA‑CCTCCG‑CCCGA‑TTCTTTCCCC‑AGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTg                                                      >  1:735847/1‑250 (MQ=255)                                                                                                                                                                                               gTTT‑GCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAA‑CCTCCG‑CCCGA‑TTCTTTCCCC‑AGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTg                                                      >  1:602011/1‑250 (MQ=255)                                                                                                                                                                                                             ccgccgCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAA‑CCTCCG‑CCCGA‑TTCTTTCCCC‑AGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGGCGATCTGCGGACCATCCTGAGTTGCAACTGCCTTCGCCTTGGc                                         >  1:1536632/1‑250 (MQ=255)                                                                                                                                                                                                             ccgccgCCCGTCTTTTAC‑CCGTC‑CCCAGGCGGGAAGTAC‑CCCCCC‑CCCGT‑TTCTTCCCCC‑AGATTCCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGc                                         <  1:362212/250‑1 (MQ=255)                                                                                                                                                                                                                ccgccCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAA‑CCTCCG‑CCCGA‑TTCTTTCCCC‑AGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCtt                                       >  1:32922/1‑249 (MQ=255)                                                                                                                                                                                                                   cccGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAA‑CCTCCG‑CCCGA‑TTCTTTCCCC‑AGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCagc                                   >  1:930438/1‑250 (MQ=255)                                                                                                                                                                                                                      gTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAA‑CCTCCG‑CCCGA‑TTCTTTCCCC‑AGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCagctag                                >  2:1401924/1‑250 (MQ=255)                                                                                                                                                                                                                           ttAC‑CCGTC‑CCCAGGCGGGCAGTAC‑CCCCCC‑CCCGA‑TTCTTCCCCC‑AAATAGCGAGAC‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGa                            <  1:1025046/249‑1 (MQ=255)                                                                                                                                                                                                                             aa‑CCGTA‑CCCAGGCGGGCAGTAA‑CCTCCG‑CCCGA‑TTCTTTCCCC‑AGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATg                         >  1:1176599/1‑250 (MQ=255)                                                                                                                                                                                                                                   cgtccccGGGGGGCAAGAA‑CCCCCC‑CCCCG‑TTTTTTCCCCAAGATATGGAGAG‑GCCAC‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATgcgc                      <  1:163534/247‑1 (MQ=255)                                                                                                                                                                                                                                     cccagggggggCAGTAC‑CCTCCC‑CCCGT‑TTCTCCCCCA‑AGAAGCCGACAT‑GCCGC‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGt                   <  2:422598/242‑1 (MQ=255)                                                                                                                                                                                                                                      cccAGGCGGGCAGTAA‑CCTCCG‑CCCGA‑TTCTTTCCCC‑AGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGtt                  >  1:1418594/1‑250 (MQ=255)                                                                                                                                                                                                                                        ccggCGGGTAGTAACCCCCCG‑CCATT‑TTCTTCCCCC‑AAATATGTAGTT‑GCCAC‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTc                 <  1:1296918/248‑1 (MQ=255)                                                                                                                                                                                                                                        cAGGCGGGCAGTAA‑CCTCCG‑CCCGA‑TTCTTTCCCC‑AGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGtt                  >  1:1266206/1‑248 (MQ=255)                                                                                                                                                                                                                                        cAGGCGGGAAGTAC‑CCCCCC‑CCCGA‑TTCTTTCCCC‑AGATATCGAGAT‑CCCAC‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCa                <  1:924321/250‑1 (MQ=255)                                                                                                                                                                                                                                          ggCGGGCAGTAA‑CCTCCG‑CCCGA‑TTCTTTCCCC‑AGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGc              >  1:172255/1‑250 (MQ=255)                                                                                                                                                                                                                                          ggCGGGCAGTAA‑CCTCCG‑CCCGA‑TTCTTTCCCC‑AGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGc              >  1:1414574/1‑250 (MQ=255)                                                                                                                                                                                                                                          ggCGGGATGTAC‑CCCCCC‑CCCGA‑TTTTTCCCCC‑AGATGCCGAGAT‑GCCGG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGc              <  1:272619/250‑1 (MQ=255)                                                                                                                                                                                                                                            aggcaaGTAA‑CCCCCG‑CCCGA‑TCTTTCCCCC‑AGATACCGAGAG‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCa            <  1:1429358/245‑1 (MQ=255)                                                                                                                                                                                                                                             ggggAGGAA‑CCCCCG‑CCAGT‑TTCTTCCCCC‑AGGTATGGGGAT‑ACCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCAc           <  1:1239787/250‑1 (MQ=255)                                                                                                                                                                                                                                              ggCAGTAA‑CCTCCG‑CCCGA‑TTCTTTCCCC‑AGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCAcc          >  1:1217233/1‑250 (MQ=255)                                                                                                                                                                                                                                              agCAGTAC‑CCTCCG‑CCTGA‑TTCTTGCCCC‑AGATAACGAGAG‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCAcc          <  1:1539345/249‑1 (MQ=255)                                                                                                                                                                                                                                                cAGTAC‑CCCCCG‑CCCGT‑TTCTTTCCCC‑AGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTg        <  1:1407198/250‑1 (MQ=255)                                                                                                                                                                                                                                                   tAA‑CCTCCG‑CCCGA‑TTCTTTCCCC‑AGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCa      >  1:425888/1‑249 (MQ=255)                                                                                                                                                                                                                                                    aa‑CCTCCG‑CCCGA‑TTCTTTCCCC‑AGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCata    >  1:759273/1‑250 (MQ=255)                                                                                                                                                                                                                                                       ccTCCG‑CCCGA‑TTCTTTCCCC‑AGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGCGTGGCCACTGCCTTCGCCTGGTCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCGGCatacc  >  2:161295/1‑248 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                 CTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTG‑CAAGACCGTGTTTAA‑CGCCGCCTGGGTT‑TG‑CCACTCCCAGTACA‑GGCGGG‑CTACGCTGCCAGCCAGCAATTGGC‑GGGTTT‑GCTCGCGTTCCGCCGCCCGTGCTTTAA‑CCGTA‑CCCAGGCGGGCAGTAA‑CCTCCG‑CCCGA‑TTCTTTCCCC‑AGATATCGAGAT‑GCCAG‑CCCGCCG‑TTAAGCCAAAAGTACCG‑TTGGTGTACCACGGGCCGGTC‑GTACCTGCGGCCGGATCGTTCAGAGCA‑AACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGTTCAGCCACCTGCATATC  >  NZ_CP009273/2221517‑2222001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑