BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,594,161	0	C	T	32.0%	209.5 / 50.9	122	S62F (TCT→TTT)	bglJ	DNA‑binding transcriptional activator BglJ
Reads supporting (aligned to +/- strand): ref base C (12/71); new base T (39/0); total (51/71)
Fisher's exact test for biased strand distribution p-value = 1.29e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 129 total aligned reads displayed.

GATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCT‑GGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGT  >  NZ_CP009273/4593914‑4594409

gATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATtttttctt                                                                                                                                                                                                                                                              <  1:1084240/250‑1 (MQ=255)

        gcgcgcAGTGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCCGATCTCGTTTGCGGCGGCTCTTtttttctctttttcc                                                                                                                                                                                                                                                       >  2:306160/1‑246 (MQ=255)

        gcgcgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCAtttttttttttttctcc                                                                                                                                                                                                                                                      >  1:951623/1‑245 (MQ=255)

         cgcgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTTTTTTTTTGccc                                                                                                                                                                                                                                                     >  1:1484718/1‑249 (MQ=255)

         cgcgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCa                                                                                                                                                                                                                                                     <  1:1301093/250‑1 (MQ=255)

               gggTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGTGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑aa                                                                                                                                                                                                                                              <  2:366556/250‑1 (MQ=255)

                  tGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTg                                                                                                                                                                                                                                           <  2:748603/250‑1 (MQ=255)

                     aaGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTCCATtaggaaggagc                                                                                                                                                                                                                                         >  2:1453496/1‑242 (MQ=255)

                      agagAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGTCCTGAG‑AAGAGAgcg                                                                                                                                                                                                                                       >  1:497272/1‑250 (MQ=255)

                       gagaACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTCTTCCCTTCG‑AAGTGAgcgc                                                                                                                                                                                                                                      >  1:62786/1‑250 (MQ=255)

                       gagaACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAgcgc                                                                                                                                                                                                                                      <  1:1506353/250‑1 (MQ=255)

                            cttaacttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGCCCTTGG‑AAGTGGgcgccgggg                                                                                                                                                                                                                                 >  2:1142305/1‑245 (MQ=255)

                              taacttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTCCATGGG‑AAGTGAGCGCAGTGAgg                                                                                                                                                                                                                               >  1:367459/1‑250 (MQ=255)

                                 cttCCCTTCTGTTTATGGAGATACCGCAGAATGGAACACAGCCGAATTAATAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGACAATTTATTCAAAAGGTTTGCAGGTAACCCTTATAAGCTCCGTTCCTATACCAGTCAGGTGTCATTTCAGGATGCCTTGTCGCGGCTCTCGTTTGCGGCGG‑TCATTGTTTCTATGTCTGCCGTGAG‑GAGTGACCGCagatttgtat                                                                                                                                                                                                                            <  2:673610/250‑8 (MQ=255)

                                  ttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGCCATGAG‑AAGTGAGGGCCGAGAGGGAtt                                                                                                                                                                                                                           >  2:565180/1‑250 (MQ=255)

                                    gCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGAtt                                                                                                                                                                                                                           <  1:559387/248‑1 (MQ=255)

                                      cTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCt                                                                                                                                                                                                                       <  1:638713/250‑1 (MQ=255)

                                              tATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑CCATTTTTTTTTTTTCTTCCCTGAGCAGGTGGCGGCGGAG‑GGGATTATCtttctctgt                                                                                                                                                                                                               >  1:161065/1‑243 (MQ=255)

                                                tGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTTTTTTTTTTCCTTGAG‑GAGTGGGCGCAGAGAGGGGTTATCTTGCctggctg                                                                                                                                                                                                             >  2:881174/1‑250 (MQ=255)

                                                tGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCctgactg                                                                                                                                                                                                             <  1:447096/250‑1 (MQ=255)

                                                tGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCctgact                                                                                                                                                                                                              <  2:984713/249‑1 (MQ=255)

                                                 ggAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTTTTTTTTTTCCCTGAG‑GAGTGGGGGCCGGGAGGGGTTTTTTTTCcttactg                                                                                                                                                                                                             >  1:817801/1‑249 (MQ=255)

                                                     aTGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTCCCTTAG‑TAGTGAGCGCCGAGAGGGGTTTTTTTGCctgtatggaatg                                                                                                                                                                                                        >  2:576407/1‑241 (MQ=255)

                                                      tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCCTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTTTTTTTTTTCCCCTGG‑TAGTGAGGGCAGGGGGGGAGTTTCTTGCctaacataacgtg                                                                                                                                                                                                       >  2:860524/1‑242 (MQ=255)

                                                       gCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑GCTTTTTTTTTTTTTTTTCCCTGAG‑AAGTGGGGGCAGAGGGGGGTTATTTTTCctgtttctaattgc                                                                                                                                                                                                      >  2:306261/1‑239 (MQ=255)

                                                           cAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑Att                                                                                                                                                                                                 <  1:811426/250‑1 (MQ=255)

                                                             gAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑aa                                                                                                                                                                                              <  1:926313/250‑1 (MQ=255)

                                                               aTGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTCTTTTTTTTCCTTGAG‑AAGTGAGGGCAGAGAGGGGTTATCTTTCCTGACTGTACTGGCgggtaaat                                                                                                                                                                                              >  2:1033316/1‑243 (MQ=255)

                                                                tGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑CCCTTTTTTCTTTTTCTTCCCCGAG‑AAGTGAGCGCCGCGAGGGGTTTTCTTTCCTGTCTGAACTGGCG‑CTT‑TAgat                                                                                                                                                                                           >  2:705733/1‑248 (MQ=255)

                                                                        aGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTCTTCCATGAG‑GAGGGAGCGCAGAGAGGGAGTATTTTGGCTGGCTGGATTGGGG‑GTT‑GAGTTTTCGCGTa                                                                                                                                                                                   >  1:313363/1‑250 (MQ=255)

                                                                         gCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAGCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGTCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTAc                                                                                                                                                                                  <  1:999809/250‑1 (MQ=255)

                                                                          ccGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTCTTTTTTTTCCATGGG‑AAGAGAGCGCAGAGAGGGGGTTTCTTTCCTTACTGAACTGGCG‑GTT‑AATATTCGGCGTAcc                                                                                                                                                                                 >  1:43777/1‑250 (MQ=255)

                                                                            gAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGCCCTGTG‑AAGAGGGCGCAGGGGGGGATTATTTTGTCTGACTGAACAGGGG‑GTTAAAGTTTcagggccccg                                                                                                                                                                                >  2:1368786/1‑241 (MQ=255)

                                                                              aTTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTCCCTGAG‑AAGTGAGCGCAGCGAGGGATTTTCTTGCCTGACTGAACTGTGG‑ATT‑AAGTTTCCGCGTACCCGGc                                                                                                                                                                             >  1:1271617/1‑250 (MQ=255)

                                                                                tAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTCTGCCCTTGG‑AAGTGAGCGCAGAGGGGGGGTGTTTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTTCGCGTACCCGGCgg                                                                                                                                                                           >  1:1385833/1‑249 (MQ=255)

                                                                                 aagaagAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGCCATGCG‑AAGTGAGCGCGGAGAGGGATTTTCTTTCCTGACTGAACTGGCG‑ATT‑TAGTTTTCGCGTACCCGGCggt                                                                                                                                                                          >  1:166879/1‑248 (MQ=255)

                                                                                  agaagaGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGttt                                                                                                                                                                         <  2:1185659/250‑1 (MQ=255)

                                                                                   gaagaGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGGGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTa                                                                                                                                                                        <  2:1154236/250‑1 (MQ=255)

                                                                                          aaTGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTg                                                                                                                                                                 <  2:430115/250‑1 (MQ=255)

                                                                                           aTGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGc                                                                                                                                                                <  2:557045/250‑1 (MQ=255)

                                                                                             gTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTCTGCCCTTAG‑AAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGGTTCCGCGTACCCGGCGTTTAGTTATTGCgg                                                                                                                                                              >  1:716161/1‑250 (MQ=255)

                                                                                                gCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatg                                                                                                                                                           <  2:748813/250‑1 (MQ=255)

                                                                                                   cTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatg                                                                                                                                                        <  2:1014768/250‑1 (MQ=255)

                                                                                                      aTAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTCTTTTTCTGCCATGAG‑AAGAGGGCGCCGAGAGGGGTTTTCTTGCCTGCCTGAAATGGCG‑ATT‑AAGTTTTCGCGTACCCGGCGTTTTGTTCTTGCGGGTGATGgta                                                                                                                                                     >  1:1074855/1‑250 (MQ=255)

                                                                                                      aTAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGata                                                                                                                                                     <  1:466373/250‑1 (MQ=255)

                                                                                                       gaGAAAAATGCGTCATGAGTAGTATCGGTATTGAGATTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGatat                                                                                                                                                    <  2:1398558/249‑1 (MQ=255)

                                                                                                        aGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTCCCTGAG‑AAGTGGGCGCAGAGAGGGGGTATCTTTCCTTACTGAAACGGCG‑ATT‑TAGTTTCTCCGTCCCCGGCGTTTTTGTATTTCGGGTGATTGTAtt                                                                                                                                                   >  2:1083419/1‑250 (MQ=255)

                                                                                                           aaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGGTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGaa                                                                                                                                                <  2:40714/250‑1 (MQ=255)

                                                                                                            aaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAg                                                                                                                                               <  1:1449059/250‑1 (MQ=255)

                                                                                                            aaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATATCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTTTTTTTTTGTCCTGAG‑GAGGGAGGGCAGAGGGGGGGTATCTTGTCTTACTGAAATGGGG‑GTT‑AAGGTTTCGCGTAACCGGGGGTTAGGTATTGGGGGTGGTGATATTGAAg                                                                                                                                               >  2:233777/1‑250 (MQ=255)

                                                                                                                   tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCt                                                                                                                                        <  2:1235174/250‑1 (MQ=255)

                                                                                                                        agtagtATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTg                                                                                                                                  <  1:662084/250‑1 (MQ=255)

                                                                                                                        agtagtATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTg                                                                                                                                  <  1:481342/250‑1 (MQ=255)

                                                                                                                        agtagtATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGACATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTg                                                                                                                                  <  2:323406/250‑1 (MQ=255)

                                                                                                                        agtagtATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGGAACCCTTATAAGCGCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GAtt                                                                                                                                   <  2:551557/249‑1 (MQ=255)

                                                                                                                                     ttGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑c                                                                                                                     <  1:1427733/249‑1 (MQ=255)

                                                                                                                                         gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCa                                                                                                                <  1:1543307/250‑1 (MQ=255)

                                                                                                                                            tttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTTTTTTTTTTCCCTGAG‑AAGTGAGCGCAGAGAGGGATTTTTTTTCCTGTCTTAACTGGCG‑GTT‑AAGTTTTCGCGTACCCCGCGTTTTGTTATTGCGGATGATGATATTTAAGATCGGGT‑GGTTGGGTTCTTTTTG‑CCCTCAccc                                                                                                             >  2:1087421/1‑249 (MQ=255)

                                                                                                                                                ttCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGGGGGGTTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTTCCCGTAACCGGCGGTTTGTTATTTGGGGTGATGGTATTTAAGCTCGGCT‑GGTTTGTTTCTTTTCG‑CCAttcacgccgg                                                                                                         >  1:1375138/1‑241 (MQ=255)

                                                                                                                                                 tCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTCCCTGAG‑AAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCG‑ATT‑TAGTTTTCGCGTACCCGGCGGTTAGGTATTGCGGGTGATGATATTTAAGCTCGGCT‑GATTTGTTCATTGTTG‑CCCTCACCCCTg                                                                                                          >  1:534757/1‑248 (MQ=255)

                                                                                                                                                    gAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACgg                                                                                                     <  2:841577/250‑1 (MQ=255)

                                                                                                                                                     aaaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTCTTCCCTGAG‑CAATGAGGGCCGAGGGGGGTTTTTTTTCCTTGCTGAAATGGCG‑ATT‑AAATTTTCCCGTACCCCGCGGTTTGGTATTGTGGGTGATGATATTTAAGATTCGCT‑GGTTTGTTCATTGTTG‑GCCTTACCCCTGGGCggg                                                                                                    >  1:538580/1‑249 (MQ=255)

                                                                                                                                                       aGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGgtgt                                                                                                  <  1:52622/250‑1 (MQ=255)

                                                                                                                                                         tttGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGGTTTCGCGGACCCCGCGTTTTGTTATTGCGGGTGGTGATATTGGAGGTCGGCT‑GATTGGTTCATTGTTG‑CCATCACCGCTGGAAGGTGGAt                                                                                                >  1:1036749/1‑250 (MQ=255)

                                                                                                                                                            gCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTTCCTTACTGAACTGGCG‑GTT‑AAGTTTCCGCGTACCCGGCGTTTTGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCCTCACCGCTGGGCGGTGGAtt                                                                                               >  1:1211871/1‑248 (MQ=255)

                                                                                                                                                              gggTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTTTTTTTTTTCCATGGG‑AAGGGAGGGCAGAGAGGGAGTTTCTTGCCTGACTGAACTGGCG‑GTT‑AAGTTTTCGCGTCTTCCGCGTTTTGTTATTGTGGGTGATGATATTGAAGATCCGAT‑GCGTGGTTCATTGTTG‑CCATCACCGCTGGGGGGTGTAGTAAg                                                                                            >  2:533412/1‑249 (MQ=255)

                                                                                                                                                               ggTAGCCCTTATAAGCTCCATGCCTATTCCAGTCCGGAATCATTTCAGGATGCCAGTTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGGTGTATTAAGTa                                                                                          <  2:1106737/250‑1 (MQ=255)

                                                                                                                                                                   aaccTTATAAGCTCCATACCTATACCAGTCATGGGTCTTTTCTGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTTACTGAACTGGCG‑ATT‑AAGTTTCCGCTTACCCGTCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGGTGTATTAAGTAAAg                                                                                       <  2:1273173/247‑1 (MQ=255)

                                                                                                                                                                   aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGGTGTATTAAGTAAAg                                                                                       <  1:1156426/249‑1 (MQ=255)

                                                                                                                                                                   aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGGTGTATTAAGTAAAGc                                                                                      <  2:155869/250‑1 (MQ=255)

                                                                                                                                                                          taAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATTTTGCCTGACTGAAACGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGGTTTGTTATTTCGGATGATGATATTTAAGCTCGGGT‑TGTTGGTTCATTTTCT‑CCACTCCCCCTGGGCGGGGGTTTAAGTAAAgaggtcaaa                                                                               >  1:1515433/1‑242 (MQ=255)

                                                                                                                                                                          taAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAAc                                                                                <  1:759915/249‑1 (MQ=255)

                                                                                                                                                                             gCTCCATGCCTATACCAGTCAGGAGTTATTACAGGATGCAATGTCGCGGATCTCGTTTGCGGCGA‑TCATTGTTTCTGTATCTGCAATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGACG‑ATT‑AAGGTTCCGCGAACCCGGCGTTTAGTTATTGCGGATGAAGATATTGAAGCTCGGGT‑GATTGGTTCATTGTCG‑CCATCACCGCTGACCGGTGTATTAAGTAAAGCGTCAACGCT‑g                                                                           <  2:386767/250‑1 (MQ=255)

                                                                                                                                                                              cTCCATTCCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCT‑gg                                                                          <  2:1312741/250‑1 (MQ=255)

                                                                                                                                                                              cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTTAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCT‑gg                                                                          <  1:1158192/250‑1 (MQ=255)

                                                                                                                                                                                 cATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCT‑Ggag                                                                        <  2:667767/249‑1 (MQ=255)

                                                                                                                                                                                 cATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCT‑Gga                                                                         <  1:165355/248‑1 (MQ=255)

                                                                                                                                                                                   tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTCTGCCATGAG‑AAGAGAGCGCCGAGAGGGGTTATCTTGTCTGACTGAACTGGCG‑GTT‑TAGGTTTCGCGGACCCCGCGGTTTGTTATTTCGGGTGATGATATTTAAGGCCCGGT‑GATTGGTTCCTTGTCG‑TCATCACCGCTGGTCGGTGTATTAAGTAATGCGTCAACGGT‑gggggtt                                                                     >  2:455844/1‑245 (MQ=255)

                                                                                                                                                                                          aCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGCCCTGGG‑AAGGGAGGGCAGGGGGGGGTTTTTTTTCCTTGCTGAACTGGGG‑GTT‑AAAGTTTCGCGGAACCGGGGGTTTGTTTTTTCGGGGGGTGGTATTTTAACTCGGCTGGTTTGGTTATTTTTGG‑CCATCCCCCCTGGAGGGGGTTTTAAGAAAGGCGTCAA‑GCTGGGGGAtttttat                                                                >  2:1102272/1‑247 (MQ=255)

                                                                                                                                                                                             aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCT‑GGAGATTTTTCATCAgg                                                           <  2:1074456/250‑1 (MQ=255)

                                                                                                                                                                                             aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCT‑GGAGATTTTTCATCAgg                                                           <  2:1089946/250‑1 (MQ=255)

                                                                                                                                                                                              gTCAGGAGTCATTTCATGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCT‑GGAGATTTTTCATCAGGa                                                          <  1:805397/250‑1 (MQ=255)

                                                                                                                                                                                               tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCT‑GGAGATTTTTCATCAGGaa                                                         <  2:393468/250‑1 (MQ=255)

                                                                                                                                                                                                    aGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCT‑GGAGATTTTTCATCAGGAACTCtt                                                    <  2:447118/250‑1 (MQ=255)

                                                                                                                                                                                                        aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTCCATGAG‑AAGTGAGCGCAGAGAGGGGGTATCTTGCCTGACTGAAATGGCG‑GTT‑TAGTTTTCGCGGACCCGGCGGTTTGTTTTTTCGGATGATGATATTGAAGACCGGCT‑GGTTTGTTCATTTTTG‑CCATTACCGCTGGGCGGGGGTTTTAGTAAAGCGTCCAACCT‑GGGGATTTTTTATCAGGAGCTCtttttt                                                >  2:1527754/1‑249 (MQ=255)

                                                                                                                                                                                                            cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑GAATTTTTTTTTTTTTTGTCCTGTG‑AAGGGAGCGCAGGGGGGGATTATCTTTGCTGACTTAACTCGCGCTTT‑AAGTTT‑CGGGTTACCGGGGGTTAGGTTTTTCGGGTGGTGGTATTTTAACTCGGGC‑GGTTGGTTTATTTTCGCCCTTCACC‑CCGGGCGGTGTTTTAAGTAAATCGGTAAAACT‑GGAGATTTTTTCTCAAGAACTCTTTTTGTcta                                            >  2:626905/1‑248 (MQ=255)

                                                                                                                                                                                                               gATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCT‑GGAGATTTTTCATCAGGAACTCTTTTTGTCATTaa                                         <  1:872754/250‑1 (MQ=255)

                                                                                                                                                                                                                aTGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTTCTGTGAG‑AAGTGAGGGCTCAGCGGGGTTATATTTCCTGGCTGCACTGTCC‑ATT‑TATTTTTCGCGTTGCCGCTGTTTAGTTTTTGTGGGTGGTAGAATTTTAGTCCGGCT‑GATTGGTCTTTTTTTG‑CCCTTACCGCTGGGCGGTGTATTTAGTTAAGGGGAAACCCC‑GGACAGTTTTTTTCACGTTCTCttttttgctgtaaa                                        >  2:1467943/1‑241 (MQ=255)

                                                                                                                                                                                                                   gcATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCT‑GGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATgg                                     <  2:913967/249‑1 (MQ=255)

                                                                                                                                                                                                                            ggATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGTCTGAACTGGCG‑ATT‑TAGTTTTCGCGTACCCGGCGGTTTGTTATTTCGGATGATGATATTTAAGCTCGGCT‑GATTGGTTTATTGTTG‑CCATCCCCGCTGGACGGGGGATTTAGTAAAACGTGCAAGCT‑GGGGGTTTTTCATCAGGAAATCTTTTTGTCATTAAATGGGTTACGTct                            >  2:491729/1‑249 (MQ=255)

                                                                                                                                                                                                                             gATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCT‑GGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAg                           <  1:701757/250‑1 (MQ=255)

                                                                                                                                                                                                                                   gTTTGTGGTGG‑TTATTTTTTCTTTTTCTGCAATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGTG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCT‑GGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAgg                          <  1:1179934/245‑1 (MQ=255)

                                                                                                                                                                                                                                        cggcgg‑TCAGTTTTTCTTTTTCTGCAGTGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCT‑GGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGTTGAACGACAGGcgaccgaccg                <  2:316938/250‑1 (MQ=255)

                                                                                                                                                                                                                                           cgg‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGCGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCCCCGCTGGACGGTGTATTAAGTAAAGCATCAACGCT‑GGAGATTTTTCATCAGGATCTCTTTTTGTCGTTACATGGTGTACGTCAGGCTACCGAGCGACt             >  2:179841/1‑250 (MQ=255)

                                                                                                                                                                                                                                                 aTTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCT‑GGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACa        <  2:1207152/250‑1 (MQ=255)

                                                                                                                                                                                                                                                  ttttttCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCT‑GGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGCCCGACCGACTGAACaa       >  1:718499/1‑250 (MQ=255)

                                                                                                                                                                                                                                                  ttttttCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCT‑GGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACaa       >  2:975205/1‑250 (MQ=255)

                                                                                                                                                                                                                                                  ttttttCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCT‑GGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACa        <  2:439441/249‑1 (MQ=255)

                                                                                                                                                                                                                                                       tctttttctGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCT‑GGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAg   <  1:1109075/249‑1 (MQ=255)

                                                                                                                                                                                                                                                       tctttttctGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCT‑GGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGt  >  2:904741/1‑250 (MQ=255)

GATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAG‑AAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCT‑GATTGGTTCATTGTCG‑CCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCT‑GGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGT  >  NZ_CP009273/4593914‑4594409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`