Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,221,718	0	G	C	36.4%	62.5 / 10.6	44	pseudogene (520/1436 nt)	mdtQ	multidrug resistance outer membrane protein MdtQ
Reads supporting (aligned to +/- strand):  ref base G (12/16);  new base C (10/6);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 3.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATAATTTTCATTTTCCCCGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑TTT‑GCT‑CGCGTTCCGCCGCCCGTG‑CTTTAACCGT‑A‑CCCAGGCGGGCA‑G‑TAACCTCCGCCCGATTCTTTCCCCAGATATCGAGA‑TGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGG‑CCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCA  >  NZ_CP009273/2221469‑2221966                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                ataatTTTCATTTTCCCCGCGACATCGTTTAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑TTT‑GCT‑CGCGTTccgccg                                                                                                                                                                                                                                                                 <  1:1012884/250‑1 (MQ=255)   aatTTTCATTTTCCCCGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑TTT‑GCC‑CGCGCTCCgccgcc                                                                                                                                                                                                                                                               >  1:676168/1‑250 (MQ=255)        tcattttcCCCGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑TTT‑GCT‑CGCGTTCCGCCGCCCGTg                                                                                                                                                                                                                                                           <  2:317086/249‑1 (MQ=255)          attttcCCCGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑TTT‑GCT‑TGGGTTCCGCCCCCCGTG‑cct                                                                                                                                                                                                                                                       >  1:120078/1‑248 (MQ=255)             ttcCCCGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑TTT‑GCT‑CGCGTTCCGCCGccccggccttta                                                                                                                                                                                                                                                     >  1:502420/1‑241 (MQ=255)             ttcCCCGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑TTT‑GCT‑CGCGTTCCGCCGCCCGTG‑CTTTa                                                                                                                                                                                                                                                     <  1:969719/249‑1 (MQ=255)              tcCCCGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑GTT‑GGT‑CGCGGTCCGCCGCCCCCG‑CTTTTAc                                                                                                                                                                                                                                                   >  1:164269/1‑250 (MQ=255)               ccccGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑TTT‑GCT‑CGCGTTCCGCCGCCCGTG‑CTTTAAc                                                                                                                                                                                                                                                   <  1:158731/249‑1 (MQ=255)                 ccGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑TTT‑GCC‑CGCGGTTCGCCGcccccgcctttaacg                                                                                                                                                                                                                                                  >  1:354496/1‑237 (MQ=255)                  cgcgACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑TTT‑GCT‑CGCGTTCCGCCGCCCGTG‑CTTTAACCGT‑a                                                                                                                                                                                                                                              <  2:980978/250‑1 (MQ=255)                  cgcgACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑TTT‑GCT‑CGCGTTCCGCCGCCCGTG‑CTTTAACCGT‑a                                                                                                                                                                                                                                              <  1:922379/250‑1 (MQ=255)                   gcgACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑GTT‑TCTCCGGGTTCCGCGCCCCTGG‑CTTTAACCGtc                                                                                                                                                                                                                                               >  2:536619/1‑249 (MQ=255)                                      gcCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑TTT‑GCT‑CGCGTTTCGCCGCCCCTG‑CTTTAACCGT‑A‑ACCACGCGGGCC‑G‑TTACCTc                                                                                                                                                                                                                       >  1:909542/1‑250 (MQ=255)                                       ccGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑TTT‑GCT‑CGCGTTCCGCCGCCCGTG‑CTTTAACCGT‑A‑CCCAGGCGGGCA‑G‑TAACCt                                                                                                                                                                                                                        <  1:362382/248‑1 (MQ=255)                                         gggTTTTGCTGGCATTAATAACGGTTTCCGCACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑TTT‑GCT‑CGCGTTCCGCCGCCCGTG‑CTTTAACCGT‑A‑CCCAGGCGGGCA‑G‑TAACCTCCGc                                                                                                                                                                                                                    <  1:974726/250‑1 (MQ=255)                                           gTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGGTTTT‑GCC‑CGGGTTCGCCCCCCCGGC‑CTTTAACCG‑‑A‑CCCAGGCGGGGC‑G‑CAACcccccccc                                                                                                                                                                                                                  >  1:1136509/1‑250 (MQ=255)                                           gTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑TTT‑GCT‑TCCGGTCCGCCCCCCGGG‑CCTTTACCGT‑A‑CCCCGGCGGGCC‑G‑GAACCTcccccc                                                                                                                                                                                                                  >  1:823239/1‑250 (MQ=255)                                            ttttGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑GTT‑GCT‑CTCGTTTCGCCCCCCCAG‑TTTTTACCGC‑A‑TCCACGCGGGCA‑G‑CAACCTccccccc                                                                                                                                                                                                                 >  1:376129/1‑249 (MQ=255)                                                 cTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑TTT‑GCT‑CGCGTTCCGCCGCCCGTG‑CTTTAACCGT‑A‑CCCAGGCGGGCA‑G‑TAACCTCCGCCCGATTCt                                                                                                                                                                                                            <  1:270076/250‑1 (MQ=255)                                                               ggTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑GTT‑TTT‑CGCGGTTCGCCCCCCCCG‑CTTTTACCGC‑A‑CCCCGGGGGGCG‑GCAAACCCCCCCCCCCTTTTTTccccggtattc                                                                                                                                                                                               >  2:575647/1‑243 (MQ=255)                                                                gTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTTGCGGGG‑TTT‑TCT‑CGCGTCCCCCGCCCCGCG‑CTTTACCCTC‑A‑CCC‑GGCGGGGC‑G‑TAATCTCCCCCCCGCTCTTTtccccccccatca                                                                                                                                                                                             >  2:797832/1‑241 (MQ=255)                                                                       cacCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑TTT‑GCT‑CGCGTTCCGCCGCCCGTG‑CTTTAACCGT‑A‑CCCCGGCGGGCA‑G‑TAACCTCCCCCCGGTTTTTTCCCCCGATATCgaaaatccc                                                                                                                                                                                      >  1:1188483/1‑243 (MQ=255)                                                                         ccTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑TTT‑GCT‑CGCGTTCCGCCGCCCGTG‑CTTTAACCGT‑A‑CCCAGGCGGGCA‑G‑Ta                                                                                                                                                                                                                            <  1:1136436/210‑1 (MQ=255)                                                                          cTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑TTT‑GCT‑CGCGTTCCGCCGCCCGTG‑CTTTAACCGT‑A‑CCCAGGCGGGCA‑G‑TAACCTCCGCCCGATTCTTTCCCCAGATATCGAGA‑TGCCAGcc                                                                                                                                                                                  <  1:549184/250‑1 (MQ=255)                                                                          cTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑GTT‑GCT‑CGCGGTCCCCCCCCCCTG‑CTTTTACCGG‑A‑CCCAGGCGGGCA‑G‑TAACCTCCGCCCCGTTCTTTTCCCCGATATCCAGA‑TTCCAGcc                                                                                                                                                                                  >  1:376227/1‑250 (MQ=255)                                                                             cAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑TTT‑GCT‑CGCGTTCCGCCGCCCGTG‑CTTTAACCGT‑A‑CCCAGGCGGGCA‑G‑TAACCTCCGCCCGATTCTTTCCCCAGATATCGAGA‑TGCCAGCccg                                                                                                                                                                                <  1:188129/249‑1 (MQ=255)                                                                                                      tattggcgggCGTTCGTTCGCGATAATGTTTTTCGGCTCTTTTTTTATTTGCTGCAAGCCCGTTTTTACCGCGGCCTGGGTTGC‑CCCCTCCCGGTACGGGCGGGCTCCCCTGCCAGCCAGAATTTGGC‑GGG‑TTT‑GCC‑CGCTTCCCGCCGCCCGTG‑CTTTACCCGT‑C‑CCCAGGGGGGCA‑G‑TACCCCCCGCCCGATTTTTCCCCCAGATATCGAGT‑TGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGgtg                                                                                                                                                      <  2:482572/242‑1 (MQ=255)                                                                                                             tGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑GTT‑TCT‑CGCGGTCCGCCGCCCCCGCCTTTAACCG‑‑A‑CCCCGGCGGGGA‑G‑TAACCCCCGCCCCGCTCTTTTCCCCCATATCCAGA‑TGGCAGCCCCCCCCTTACACCAAAGTACCCTTTGTGTTCCCCg                                                                                                                                               >  2:164539/1‑250 (MQ=255)                                                                                                             tGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGATGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑GTT‑TCT‑CGCGGTCCCCCGCCCCCGCCTTTACCCGC‑A‑CCACGGGGGGAG‑G‑GAACC‑CCCCCCCCTTTTTTCCCCCCATATCGCGA‑AGGCAGCCCCCCCGTTAGCCCAAAAAACCGGTGGTGTagcccg                                                                                                                                               >  2:356713/1‑245 (MQ=255)                                                                                                                          cgATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑GTT‑TGT‑CGCCGTCCGCCCCCCCTG‑CTTTTACCCT‑ACCCCAGCGGGGCG‑G‑AAACCCCCCCCCCTTCTTTTCCCCACAATCCGGAA‑GGCCACCCCCCCCTTAACCCAAAAAACCCCTGGGGGTACCACAGGCgggcgtcac                                                                                                                                   >  2:1041759/1‑244 (MQ=255)                                                                                                                                               tttCTATTTACTGCAAGACCGTGTTTAACGCCGCTTGGGTTTC‑CCACCCCCGGTACGGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑TTT‑GCT‑CGCGTTCCGCCGCCCGTG‑TTTTAACCGT‑C‑CCAAGGGGGGCA‑G‑TAACCTCCGCCCGATTCTTTCCCCAGATATCGAGA‑TGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGG‑CCGGATCGTTCagag                                                                                                             <  1:273065/249‑1 (MQ=255)                                                                                                                                                              agcccGTGTTAACCGCCGCCGGGGTTTG‑CCACTCCCAGAACGGGGGGGCTCCGCGGCCAGCCAGCAGTGGGC‑GGT‑TTT‑GCC‑CGCGTCCCGCCGCCCTTG‑CTTTACCCGT‑A‑CCCAGGCGGGCA‑G‑TACCCCCCGCCCTATTCTTCCCCCAGATATCGAGA‑GGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGG‑CCGGATCGTTCAGAGCAAACGGCCCCATTaa                                                                                             <  2:32964/247‑1 (MQ=255)                                                                                                                                                               gACGGTGTTTACCGCCGCCTGGGTTGC‑CCACCCCCAGTCCAGGGGGGCGCCGCGGCCAGCCGGCAATGGGC‑GGG‑TTT‑GCT‑CGCTTCCCCCCCCCCGTT‑TTTTACCCGT‑C‑CCCGGGCGGGCA‑G‑TACCCCCCGCCCGTTCCTTCCCCCAGATATAGAGA‑TCCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGG‑CCGGATCGTTCAGAGCAAACGGCCCCATTAAg                                                                                            <  2:120078/250‑1 (MQ=255)                                                                                                                                                                              cccccgggTTTGCCCCCCCCCAGGCCGGGCGGCTTCCGCTGCCGGCC‑GTCATTGGG‑GGG‑TTTGGCC‑CGC‑TTCCCCCCCCCCTT‑GTTTAACCGT‑C‑CCCCGGGGGGCAGG‑TACCCCCCCCCCTTTTTTTCCCCCAGTAAGGGAGA‑TGCAGGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGG‑CCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACAt                                                                              <  2:605255/245‑1 (MQ=255)                                                                                                                                                                                   ggttttG‑CCGCCCCCAGTACAGGCGGGCTCCGCTGCCAGCCAGCAATTGGG‑GGG‑TTT‑GCC‑CGCGTCCCGCCCCCCGTG‑CTTTAACCGT‑C‑CCCAGGCGGGCA‑G‑TACCCTCCGCCCGATTCTTTCCCCAGATATCGAGA‑TGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGG‑CCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTg                                                                        <  1:410122/247‑1 (MQ=255)                                                                                                                                                                                          gccccccccAGCACGGCCGGCCTCCGCTGCCAGCCAGCATTGGGG‑GGG‑TTG‑CCC‑CCCGTCCCGCCCCCCGTG‑TTTAACCCGT‑A‑CCCGGGCGGGCA‑G‑CACCCCCCCCCCTATCCTTCCCCCAGATACCGAGA‑TGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGG‑CCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTc                                                                  <  2:258276/244‑1 (MQ=255)                                                                                                                                                                                            cACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑GTT‑GCC‑CCCGGTTCGCCCCCCCCG‑CTTTTACCCTAA‑CCCCGCGGGGCG‑G‑CACCCCCCCCCCCCTCCTTCCCCCCAAAATC‑AGACGGCCAGCCCCCCGTTAACCCAAAA‑CACCCCTGGGGTCACCCAGGGCGGGGGGACCCCCGGCCCGCACCGTTCGGGCCAAACGGCCCCATTCAGCCTTCCGCCGCCCTTTTTT‑CCCCTCCa                                                                >  2:64010/1‑250 (MQ=255)                                                                                                                                                                                            cACCCCCAGTAAGGGCGGGCTCCGCTGCCAGCCAGCAATTGGC‑GGT‑TTG‑GCT‑CGCTTCCCGCCGCCCGTG‑TTTTAACCGT‑A‑CCCAGGCGGGAA‑G‑TAACCCCCGCCCGTTTCTTCCCCCAGATATCGAGA‑TGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGG‑CCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCa                                                                <  1:177637/250‑1 (MQ=255)                                                                                                                                                                                                                    cTGCCAGCCAGCAATTGGC‑GGG‑TTT‑GCT‑CGCGTTCCGCCGCCCGTG‑CTTTAACCGT‑A‑CCCAGGCGGGCA‑G‑TAACCTCCGCCCGATTCTTTCCCCAGATATCGAGA‑TGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGG‑CCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCg                                        >  1:810852/1‑250 (MQ=255)                                                                                                                                                                                                                    cTGCCAGCCAGCAATTGGC‑GGG‑GTT‑GCT‑CGCGTTCCGCCGCCCGTG‑CTTTAACCGT‑A‑CCCAGGCGGGCA‑G‑TAACCTCCGCCCGATTCTTTCCCCCGATATCGAGA‑TGCCAGCCCCCCGTTAAGCCAAAAGTACCGTTGGGGTACCACGGGCCGGGCGTACCTGCGG‑CCGGATCGTTCCGAGCAAACGGCCCCACTACGCCCTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAATCGACCTGCg                                        >  2:35204/1‑250 (MQ=255)                                                                                                                                                                                                                                          g‑TTT‑CCT‑CGCTTTCCCCCCCCCGTG‑TTTTACCCGT‑A‑CCCAGGCGGGAA‑G‑TACCCTCCCCCCGTTTTTTCCCCCAAATATGGGGA‑TGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGG‑CCGGATCGTTCAGAGCAAATGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTg                   <  1:606910/250‑1 (MQ=255)                                                                                                                                                                                                                                           gtttcccT‑CGCTTCCCCCCCCCCGT‑‑CCTTAACCCT‑ACCCCGGGGGGGCA‑G‑TCCCCCCCCCCCTTTTCTTCCCCAGGTTCCCGAGG‑TGCCACCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGG‑CCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACt                    <  2:964753/244‑1 (MQ=255)                                                                                                                                                                                                                                             gttgccccgcgTCCCGCCGCCGGTG‑CTTAAACCGT‑C‑CCCGGGCGGGCA‑G‑TAACCTCCCCCCGATTTTTCCCCCAGATAGCGAGA‑GCCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGG‑CCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTg                   <  1:378126/243‑1 (MQ=255)                                                                                                                                                                                                                                              t‑GCT‑CGCGTTCCGCCGCCCGTG‑CTTTAACCGT‑A‑CCCAGGCGGGCA‑G‑TAACCTCCGCCCGATTCTTTCCCCAGATATCGAGA‑TGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGG‑CCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCt                >  1:480946/1‑250 (MQ=255)                                                                                                                                                                                                                                                 cT‑CGCGTTCCGCCGCCCGTG‑CTTTAACCGT‑A‑CCCAGGCGGGCA‑G‑TAACCTCCGCCCGATTCTTTCCCCAGATATCGAGA‑TGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGG‑CCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTc              >  1:769700/1‑250 (MQ=255)                                                                                                                                                                                                                                                  t‑CGCGTTCCGCCGCCCGTG‑CTTTAACCGT‑A‑CCCAGGCGGGCA‑G‑TAACCTCCGCCCGATTCTTTCCCCAGATATCGAGA‑TGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGG‑CCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCg             >  1:313270/1‑250 (MQ=255)                                                                                                                                                                                                                                                           cgccgcCCGTG‑CTTTAACCGT‑A‑CCCAGGCGGGCA‑G‑TAACCTCCGCCCGATTCTTTCCCCAGATATCGAGA‑TGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGG‑CCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCt     >  2:192578/1‑250 (MQ=255)                                                                                                                                                                                                                                                            cccgccCGTC‑CTTAACCCGT‑C‑CCCGGGCGGGCA‑G‑TACCCCCCCCCCGATTTTTCCCCCAGATACCGAGA‑GGCCGGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGG‑CCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCtt    <  1:164539/249‑1 (MQ=255)                                                                                                                                                                                                                                                             ccgccCGTG‑CTTTAACCGT‑A‑CCCAGGCGGGCA‑G‑TAACCTCCGCCCGATTCTTTCCCCAGATATCGAGA‑TGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGG‑CCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTc   >  2:636046/1‑250 (MQ=255)                                                                                                                                                                                                                                                              cgccCGTG‑CTTTAACCGT‑C‑CCCAGGCGGGGA‑A‑TACCCCCCCCCCGATTCTTCCCCCAGATACCGAGA‑TCCCACCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGG‑CCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCa  <  1:606192/250‑1 (MQ=255)                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                ATAATTTTCATTTTCCCCGCGACATCGTTGAGCTGCTGCCGGGTTTTGCTGGCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTG‑CCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGC‑GGG‑TTT‑GCT‑CGCGTTCCGCCGCCCGTG‑CTTTAACCGT‑A‑CCCAGGCGGGCA‑G‑TAACCTCCGCCCGATTCTTTCCCCAGATATCGAGA‑TGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGG‑CCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCA  >  NZ_CP009273/2221469‑2221966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑