Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,245,9890CT32.7% 80.0 / 13.7 55E136K (GAA→AAA) plsBglycerol‑3‑phosphate 1‑O‑acyltransferase PlsB
Reads supporting (aligned to +/- strand):  ref base C (12/25);  new base T (18/0);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 8.29e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGT‑GCATACGCGCCA‑CGCGCGCCAGTTTCTGAGCGATAG‑TTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTCG‑CCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAAT‑CGAGATAGTCGTGGAACAGCTTAATAGACT‑CTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGT  >  NZ_CP009273/4245742‑4246232
                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     
ttaAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGT‑GCATACGCGCCA‑CGCGCGCCAGTTTCTGAGCGATAG‑TTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTCG‑CCTTTTTCAc                                                                                                                                                                                                                                                     <  1:319680/250‑1 (MQ=255)
  aaaCAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGT‑GCATACGCGCCA‑CGCGCGCCAGTTTCTGAGCGATAG‑TTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTCG‑CCTTTTTCACg                                                                                                                                                                                                                                                    <  1:359535/249‑1 (MQ=255)
                gAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGT‑GCATACGCGCCA‑CGCGCGCCAGTTTCTGAGCGATAG‑TTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTGCAC‑TCG‑CCTTTTTCCACCCCCGtcgcgggccc                                                                                                                                                                                                                                     >  2:900496/1‑245 (MQ=255)
                      gaaaccGTGGCCCTACGGCGGCCGGCCGTTGAGGGGCAAAGT‑GCAT‑CGCGGCACCGCGCGCCAGTTTCTGAGCGATAG‑TTTTATCCGTGCCGGGTCCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTCG‑CCTTTTTCACGCCCCGGCGCGCGACCAAACAt                                                                                                                                                                                                                               <  2:684093/245‑1 (MQ=255)
                        aaaCGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGT‑GCATACGCGCCA‑CGCGCGCCAGTTTCTGAGCGATAG‑TTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGGTCACTTCGCCCTTTTTTAC‑CCCCGCCGCGCGGCCCAACACCa                                                                                                                                                                                                                             >  2:218271/1‑250 (MQ=255)
                          aCGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGT‑GCATACGCGCCA‑CGCGCGCCAGTTTCTGAGCGATAG‑TTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGGTGACCTCC‑GCCTTTTCTCTCCCCCGCGCCCGGCCacaccacacc                                                                                                                                                                                                                           >  2:189699/1‑250 (MQ=255)
                               ggCCTACGGCAGCCAGACGTTGACGGGCAAAGT‑GCATACGCGCCA‑CGCGCGCCAGTTTCTGAGCGATAG‑TTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTCG‑CCCTTTTTACGCCCCCGCGCGCGCCCcaaaaccccccaccc                                                                                                                                                                                                                      >  1:485346/1‑238 (MQ=255)
                                gCCGACGGCAGCCGGGCGTTGGCGGACAAGGG‑GCATACGCCCCG‑CGCGCGCCAGTTTCGGCGCGTAAT‑TTTTTCCCGGGCGGTGTTCCTCCGCCATCCGGCGCAGCAAAACTGACGGCGAAAACCGCACAAAACTGTCGCGCCCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTCG‑CCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACt                                                                                                                                                                                                                     <  2:647547/250‑1 (MQ=255)
                                ccttggggCAGCCAGCTGTTGAGGGACAAAGT‑GCACCCGCGCCA‑CGCGCCCGAGTTTCGGAGCGATAT‑TTTTACCCGCGCCGTGTCCACCCGCCATCCGGCGCAGCGAAACGGACGGCGAGAAACGCACAAAACTGTCGCGCCCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTCG‑CCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACt                                                                                                                                                                                                                     <  2:33499/244‑1 (MQ=255)
                                      ggCAGCCAGACGTTGACGGGCAAAGT‑GCATACGCGCCA‑CGCGCGCCAGTTTCTGAGCGATAG‑TTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTTCCCTT‑G‑CCTTTTTCCCCCCCCCGCGCGCGCCCCCACAACACCCCCACCGGcccc                                                                                                                                                                                                               >  2:529158/1‑250 (MQ=255)
                                      ggCAGCCAGACGTTGACGGGCAAAGT‑GCATACGCGCCA‑CGCGCGCCAGTTTCTGAGCGATAG‑TTTTATCCGTGCCGTGTTCATCCACCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTCG‑CCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCAcc                                                                                                                                                                                                               <  1:430652/250‑1 (MQ=255)
                                                agttgggcggCAAAGGGGCATCCGCCCCC‑CGCGCCCCAGTTTCTGAG‑GATTGTTTTTACCCGGCCCGTTTTCTCCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAACCTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTCG‑CCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGcaca                                                                                                                                                                                                      <  2:791067/242‑1 (MQ=255)
                                                  ttGACGGGCAAAGG‑GCATCCGCGCCA‑CGCGCGCCAGTTAGTGGGCGATAG‑TTTTATCCGTGCCGTGTGCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTCG‑CCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATcc                                                                                                                                                                                                   <  2:374233/250‑1 (MQ=255)
                                                     aCGGGCAAAGT‑GCATACGCGCCA‑CGCGCGCCAGTTTCTGAGCGATAG‑TTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTCG‑CCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGa                                                                                                                                                                                                <  1:472301/250‑1 (MQ=255)
                                                        ggCAAGGG‑GCATCCCCGCCG‑CGCGCGCCAGTTTCTGAGCAATAG‑TTTTACCCGTGCCGTTTTCACCCGCCATACGGCGAAGCGAAACTGACGGCGAGAACCGCACAAACCTGCCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTCG‑CCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGAttt                                                                                                                                                                                             <  2:760619/250‑1 (MQ=255)
                                                          cAAAGT‑GCATACGCGCCA‑CGCGCGCCAGTTTCTGAGCGATAG‑TTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGGTCACTTTG‑CCTTTTTTACGCCCCCGCGCGCGACCACACATCACCGACACTGCCACCCCCTCCACATCCCGAttttg                                                                                                                                                                                           >  2:851706/1‑248 (MQ=255)
                                                               t‑GCATCCGCGCCA‑CGCGCGCCAGTTCCTGAGCGATAG‑TTTTACCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGCCCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTCG‑CCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGc                                                                                                                                                                                      <  1:688720/250‑1 (MQ=255)
                                                                     aCGCGCCA‑CGCGCGCCAGTTTCTGAGCGATAG‑TTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTAC‑CCTTTTTTACGCCCCGGCGCGCGAACAAAAAACACCCACACTGGCCCCATCTTCACATCCAGATTTGGGTTggttcag                                                                                                                                                                                 >  1:130165/1‑244 (MQ=255)
                                                                          ccA‑CGGGCGCCCGTTTCCGACCGATAG‑TTTTACCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTCG‑CCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCaa                                                                                                                                                                            <  2:290752/250‑1 (MQ=255)
                                                                           cA‑CGCGCGCCAGTTTCTGAGCGATAG‑TTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTCG‑CCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCaaa                                                                                                                                                                           <  1:285837/250‑1 (MQ=255)
                                                                             ccccgcccCAGTTGCGGTGCGATAG‑TTTTATCGGTGCCGTGTCCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTCG‑CCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAAt                                                                                                                                                                          <  2:645119/247‑1 (MQ=255)
                                                                               gggcgaCAGTTTCGGAGGGATAG‑TTTTATCCGTGCCGGGTCCATCCGCCATACGGCGCAGCGAAACGGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTCG‑CCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAAT‑Cg                                                                                                                                                                       <  2:720586/248‑1 (MQ=255)
                                                                               gcgcgcCAGTTTCTGAGCGATAG‑TTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGGTCACTTCG‑CCTTTTTTACGCCCCCGCGCGCGGCCCAACATCACCGCCACCGGCACCATCTGCACATCCAGATTTGGGGTGCTACTGTGCaaa                                                                                                                                                                           >  1:29324/1‑247 (MQ=255)
                                                                                 gcgcCAGTTTCGGAGGGATAG‑TTTTACCGGGGCGGTGTTCACCCGCCATACGGCGCGGAGAAACTGACGGCGAGAAACGCACAAAACGGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTCG‑CCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAAT‑Cgag                                                                                                                                                                     <  2:719724/250‑1 (MQ=255)
                                                                                                cGATAG‑TTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTCG‑CCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAAT‑CGAGATAGTCGTGGAACAg                                                                                                                                                      <  2:393468/250‑1 (MQ=255)
                                                                                                 gATAG‑TTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTCG‑CCTTTTTTACGCCCCCGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACAACCAGATTTTGGGTGCTACCGTGCAAAA‑CGGGAAAATCGTGGAACAGc                                                                                                                                                     >  1:698844/1‑250 (MQ=255)
                                                                                                        tttATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACG‑‑GAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGGTTCCTTTG‑CCTTTTTTTCGCCCCCGCGCGCGGCCAAACAACCCCCCCCACGGCCCCATCTGCACCTCCAGAGTTGGGGTGCTACGGGGCAAAA‑CGGGAGAGTCCGGGGACCGCTTAATaaa                                                                                                                                             >  2:307840/1‑248 (MQ=255)
                                                                                                          tacccgtgccgttGTCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACGGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTCG‑CCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAAT‑CGAGATAGTCGTGGAACAGCTTAATAg                                                                                                                                              <  2:173200/246‑1 (MQ=255)
                                                                                                               gccccgtgTTCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAACCTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTCG‑CCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAAT‑CGAGATAGTCGTGGAACAGCTTAATAGACt‑ctt                                                                                                                                       <  2:883543/247‑1 (MQ=255)
                                                                                                                 gccgtgTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTCG‑CCTTTTTTACGCCCCGGCGCGCGGCCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTTGGTTGCTACGGGGCAAAT‑CGAGATAGTCGTGGAACAGCTTAAAAGACt‑cttct                                                                                                                                     >  1:602569/1‑250 (MQ=255)
                                                                                                                       ttCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGGTCACTTTG‑CCCTTTTCCCCCCCCCGCGCGCGAGCCAAAATCACCGGCACTGGCCCCACCCGCACATCCCGAGTTTGGTTGGCACGGGAGAAAT‑TGACAAAGTAGGGGGACAGCCTCCTAGACTACTTTTTTCgc                                                                                                                                >  2:165491/1‑249 (MQ=255)
                                                                                                                       ttCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGGTCAATTCG‑CCTTTTTTCCGCCCCCGCGCGCGCCCAAACATAACCGACCCTCTCACCATATGCACATCCAGATTTGGGTTGCTACTGTGGAAAT‑TGAGATAGTCGTGGAAACGCTCAAAAGAAT‑CTCTTTTCTGc                                                                                                                               >  2:339548/1‑250 (MQ=255)
                                                                                                                            ccGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTCG‑CCCTTTTCACCCCCCCGCGCGCGGCCAAACATCACCGACCCCGGCCCCCTCTGCCCATCCCGATTTGGGGTGCTACCGGGCCAAA‑CGCGGTAATCGTGGGACAGCTTATAAAACT‑CCTCTTTCTGCgtgt                                                                                                                           >  2:242304/1‑249 (MQ=255)
                                                                                                                                cATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGGTCACTTCG‑CCTTTTTTCCGCCCCCCCGCGCGACCAAACATCACCGCCACTTGCCCCATCTGCACATCCCGCGTTGGGGTTGTACAGCGCAAAT‑CGAGATAGTCGTGGGAACGCTTAATAGAAT‑CTTTTTTCTGCGGGtagtag                                                                                                                      >  2:361190/1‑250 (MQ=255)
                                                                                                                                           gCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGGTCACATCG‑CCTTTTTTCAGCCCCCGCCCGCGACCAACCAACAACCACACCGGCCCCACCCGCCCCTCCCGATTTGGGGTGCTTCGGGGGTAATCCGAGCAGGACTTGGACCAGCTTATTA‑ACT‑TCTCTTTTCGCGCGTAATAAGGGAacaacac                                                                                                           >  2:617366/1‑246 (MQ=255)
                                                                                                                                                    gcGGCGAGAAACGCACAAAATTGTCCCGCCCGAGCCACAGGACAGCGAAAAATTTTTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTCG‑CTTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAAT‑CGAGATAGTCGTGGAACAGCTTAATAGACT‑CTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCccgcc                                                                                                  <  2:755533/249‑1 (MQ=255)
                                                                                                                                                    aCGGCAAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATCCGCAGCGGC‑GGGTTCACTTCG‑CCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAAT‑CGAGATAGTCGTGGAACAGCTTAATAGACT‑CTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCccgcc                                                                                                  <  1:167773/250‑1 (MQ=255)
                                                                                                                                                           gaAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACCTTG‑CCTTTTTTTCGCCCCGGCGCGCGACCAAACATCACCGACCCCGGCACCATCTGCACATCCAGAATTTGGTTGCTACGGTGCAAAT‑CGAGATAGTCGTGGGACAGCCTAATAAACT‑CTTCTTTCTGCGGGGAATAAGTGAACACCAGCGGCCCGCCGtgaat                                                                                            >  2:663105/1‑249 (MQ=255)
                                                                                                                                                            aaaCGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTCG‑CCTTTTTTCCGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCCTCTGCACATCCAGATTTGGGTTGCTACGGTGCAAAT‑CGAGATAGTCGTGGAAAAGCTTAATAGACT‑CTTCTTTCGGCGGGGAATAGGTGAACACACCCGGCCCCCCGtgaaag                                                                                           >  1:241556/1‑247 (MQ=255)
                                                                                                                                                               cGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGGTCACTTCC‑CCTTTTTTACGCCCCCGCGCGCGCCCCAACAACACCGACACCGGCCCCAACTTCACATCCCGATTTTGGGTGCTACGGTGCAAAT‑CGGGATAGACGGGGGAAAGCTTAATAAACT‑CTTCTTTCCGCGTGGAATAAGGGAAACCACCCGGcccccccgggatggaca                                                                                       >  2:125104/1‑239 (MQ=255)
                                                                                                                                                                gCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGGTCACTTCG‑CCCTTTTTACGCCCCCGCGCGCGGCCCAACATCACCGACACCGGCCCCATCTGCACCTCCAGATTTGTGGTGCTACGGTGCAAAT‑CGAGATAGGCGTGGGACAGCGTAATAGAAT‑ACTCTTTCGGCGCGTAATAGGGGAACAACAGAGGCCCGCCGTGGATGAaca                                                                                       >  2:119805/1‑249 (MQ=255)
                                                                                                                                                                                  ggAGCCACAGTACAGCAAAAATTTTCTGTACGCCGTAACGCATCCGCGGCGGC‑GGGTTCTCTCCC‑CCTTTTCCCCCCCCCGGCGCGCGCCAAAACACCCCCGCCCCTGGCCCCATCTGCACACCCAGTTTTGGTTTGCTCGGGGGCAAAG‑CGAGATAGTCGTGAAACAGCTTAATAGCCT‑CTTCTTGCGGCGTGTAATAGGTGAACACCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGt                                                                    <  2:189451/249‑1 (MQ=255)
                                                                                                                                                                                    aGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGGTCCCTTCG‑CCTTTTTTACGCCCCCGCGCGCGACCAAACATCACCGACACTGGCACCCTCTTCCCATCCCGATTTTGGGTGCTACGGTGCAAAT‑CGAGATAGTCGGGGAACAGCTTAATAGACT‑CTTCTTTCGGCGGGTAATAAGTGGACACACGCGGCCCCCCCGGAATGAAAACATAGCGCGGGCGGAGCGTGc                                                                  >  2:256244/1‑250 (MQ=255)
                                                                                                                                                                                       cacaGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTTACTTCA‑CCTTTTTTACGCCCCGGCGCGCCGCCCAACATCACCGACACTGGCACCCTCTGCACATACAGATTTTGGTTGCTACCGTGCAAAA‑CGAGATAGTCGTGGGAAAGCTTAATAAACT‑CTTCTTTTGGCGTGTAATAAGGGAACACACCCGGCCCGCCGTGAATGAACACAAAGCACGGGAGGAGCGCGCCgc                                                               >  2:322206/1‑249 (MQ=255)
                                                                                                                                                                                        acaGCACAGAAAAAATTTTCTGTCCGCCTTAAACCATCCGGAGCGGG‑GGTTTCCCTCCG‑CTTTTTCCACCCCCGGGCGCGCCACAAAACACCCCCGACACGGCCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAAT‑CGAGATAGTCGTGGAACAGCTTAATAGACT‑CTTCTTTCGGCGTGTAATAGGTAAACCCACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTc                                                              <  2:194340/250‑1 (MQ=255)
                                                                                                                                                                                                 caaaaatTTTCTTTCCGCCGTTAAGCATCCGCAGGGGG‑GGTTTCCCTCCC‑CCTTTTCCCCGCCCCGGCGCGCGACCAAACACCCCCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAAT‑CGAGATAGTCGTGGAACAGCTTAATAGACT‑CTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAg                                                     <  1:215497/248‑1 (MQ=255)
                                                                                                                                                                                                    aaaaTTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTTACTTTT‑CCTTTTTTACGCCCCGGCGCGCGGCCAAACATCACCCACCCCGGCACCATCTGCCCATCCAGATTTGGGGTGCTACGGGGCAAAA‑CGAGATAGTCGTGGGACAGCTTAATAGACT‑CTTCTTTCTGCGTGTAATAAGTGAACACACGCGGCCCGCCGTGAATGAAAACAAAACGCGGCCGGAGCGTGGCGGCGGTTTTCAACgg                                                  >  2:129017/1‑250 (MQ=255)
                                                                                                                                                                                                                  ccGTGAAGCATACGCAGCGGC‑GGTTTCACTCCG‑CTTTTTTCCCCCCCCGGCGCGCGACAAAACACCCCCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAAT‑CGAGATAGTCGTGGAACAGCTTAATAGACT‑CTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAg                                    <  1:740782/250‑1 (MQ=255)
                                                                                                                                                                                                                                    ggc‑ggGTTCACTTCG‑CCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACCGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAAT‑CGAGATAGTCGTGGAACAGCTTAATAGACT‑CTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGCGAATGAACACATAGCGCGGCAGAAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCCGGCAAGTCATGTGCCAGac                  >  2:122153/1‑250 (MQ=255)
                                                                                                                                                                                                                                      c‑ggGTTCACTTCG‑CCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAAT‑CGAGATAGTCGTGGAACAGCTTAATAGACT‑CTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCCGGCAAGTCATGTGCCAGacac                >  2:405360/1‑250 (MQ=255)
                                                                                                                                                                                                                                                ttCG‑CCTTTTTCATGCCCCGGCGCGCGACCAAACATCACCGACACCGGCACCATCTGCACATCCAGATTTGGGCTGCTACGGTGCAAAT‑CGAGATAGTCGTGGAACAGCTTAATAGACT‑CTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGgcgcg        >  2:51129/1‑249 (MQ=255)
                                                                                                                                                                                                                                                ttCG‑CCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAAT‑CGAGATAGTCGTGGAACAGCTTAATAGACT‑CTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGgcgcg        >  2:794419/1‑249 (MQ=255)
                                                                                                                                                                                                                                                   g‑CCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAAT‑CGAGATAGTCGTGGAACAGCTTAATAGACT‑CTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAAc    >  1:19399/1‑250 (MQ=255)
                                                                                                                                                                                                                                                     ccTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAAT‑CGAGATAGTCGTGGAACAGCTTAATAGACT‑CTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACg   >  1:423434/1‑250 (MQ=255)
                                                                                                                                                                                                                                                       tttttCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAAT‑CGAGATAGTCGTGGAACAGCTTAATAGACT‑CTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGt  >  2:83232/1‑249 (MQ=255)
                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     
TTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGT‑GCATACGCGCCA‑CGCGCGCCAGTTTCTGAGCGATAG‑TTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGC‑GGGTTCACTTCG‑CCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAAT‑CGAGATAGTCGTGGAACAGCTTAATAGACT‑CTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGT  >  NZ_CP009273/4245742‑4246232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑