breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 874,860 G→A M221I (ATG→ATA yliI → aldose sugar dehydrogenase YliI
RA 915,286 C→T T199I (ACT→ATT)  macA → macrolide transporter subunit MacA
RA 1,431,900 G→A Q1048* (CAG→TAG)  nifJ ← pyruvate:ferredoxin (flavodoxin) oxidoreductase
RA 3,794,146 C→A K60N (AAG→AAT rfaY ← lipopolysaccharide core heptose(II) kinase RfaY
RA 3,994,633 A→C *19S (TAA→TCA)  ysgD → protein YsgD
RA 4,322,097 C→A L334F (TTG→TTT pmrB ← two‑component system sensor histidine kinase PmrB

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 387322–388428 393888 5461–6567 42 [41] [0] 96 [BW25113_RS01920]–[yaiW] [BW25113_RS01920],BW25113_RS01920,BW25113_RS01930,iprA,ampH,sbmA,[yaiW]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =52 (0.550)56 (0.600) 49/496 2.0 50.0% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 60 (0.640)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 = 388422NA (NA)96 (1.020) 89/496 0.1 100% intergenic (‑65/‑7) BW25113_RS01920/BW25113_RS01930 IS3‑like element IS3 family transposase/autotransporter outer membrane beta‑barrel domain‑containing protein
?NZ_CP009273 393889 = 0 (0.000)coding (562/1095 nt) yaiW surface‑exposed outer membrane lipoprotein YaiW
* ? NZ_CP009273 1203246 =13 (0.140)58 (0.660) 56/464 1.1 86.5% coding (290/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 1205075 = 6 (0.070)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 120326113 (0.140)80 (0.910) 73/464 0.3 89.8% coding (305/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 = 1205058 6 (0.070)coding (57/540 nt) BW25113_RS06005 phage tail protein