Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	914,557	Δ1 bp	intergenic (‑17/‑134)	ybjX ← / → macA	VirK/YbjX family protein/macrolide transporter subunit MacA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	914,557	0	C	.	100.0%	350.6 / NA	73	intergenic (‑17/‑134)	ybjX/macA	VirK/YbjX family protein/macrolide transporter subunit MacA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (30/43);  total (30/43)

CGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTACGTTATGGAGAATTCTTAACGCACCCGTGCCGACTTAT  >  NZ_CP009273/914314‑914798                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  cgcgTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTc                                                                                                                                                                                                                                           <  1:363068/250‑1 (MQ=255)          aaTACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCAcc                                                                                                                                                                                                                                  <  2:278975/250‑1 (MQ=255)           aTACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACACGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCa                                                                                                                                                                                                                                 <  1:225670/250‑1 (MQ=255)             aCATTTAGATTAGGCCAGGGGGGAAGGTCGTTTATCCACTCCACGCTTAAAAGCGGGATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAAATTACCCGGCCCACACTGTGCAAGTGCCAGACTAAGAAAAAAAGACAGGCGGCGGAGAGATTCAAATGGGGGAAAGGTGCGTTCAGTTAGCGGGGGAAT‑TTCATGAAATTAAAGGGTATA‑ACTGCGTCGATTTCACCAtt                                                                                                                                                                                                                               >  2:442994/1‑250 (MQ=255)              cATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTg                                                                                                                                                                                                                              >  1:130987/1‑250 (MQ=255)               aTTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGc                                                                                                                                                                                                                             <  2:314424/250‑1 (MQ=255)                   agaTTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTAt                                                                                                                                                                                                                         <  1:592773/250‑1 (MQ=255)                     aTTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGCGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGTTTTCACCATTGCGTATCt                                                                                                                                                                                                                       >  2:101895/1‑250 (MQ=255)                         ggacAGAGGGAAAGTGCTTGAATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAACATTTCTGGGGAAAACTACGGCGAAGCCAGAATTTACCCGGCCGATACTGTCCACGTGCCAGACTAAGAAAAAGAGACAGGCTGCTGGGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAACTGCGACAT‑TTCATGAAATCAATGGATATA‑ATGGCGTCGATTTCACCATTGCGTATCTTaa                                                                                                                                                                                                                    <  2:583053/246‑1 (MQ=255)                           ccAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCa                                                                                                                                                                                                                 <  1:400637/250‑1 (MQ=255)                              gTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCaaa                                                                                                                                                                                                               >  1:374665/1‑249 (MQ=255)                               tGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACa                                                                                                                                                                                                             >  1:360382/1‑250 (MQ=255)                                 ggAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATc                                                                                                                                                                                                           <  1:290992/250‑1 (MQ=255)                                   aaaGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCaa                                                                                                                                                                                                         <  2:39684/250‑1 (MQ=255)                                         cgttcATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAgtgt                                                                                                                                                                                                   >  1:550963/1‑250 (MQ=255)                                          gttcATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAgtgt                                                                                                                                                                                                   >  1:614920/1‑249 (MQ=255)                                             cATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGAt                                                                                                                                                                                                >  1:525492/1‑249 (MQ=255)                                              aTCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTa                                                                                                                                                                                              >  1:593264/1‑250 (MQ=255)                                                      aaCGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACg                                                                                                                                                                                        <  1:30842/248‑1 (MQ=255)                                                            tAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAAttt                                                                                                                                                                                 >  2:354340/1‑249 (MQ=255)                                                                       tAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTttgtt                                                                                                                                                                     <  2:85992/250‑1 (MQ=255)                                                                       tAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAAACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTttgtt                                                                                                                                                                     <  1:124201/250‑1 (MQ=255)                                                                          tCAACGAGCTCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAAAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGat                                                                                                                                                                  <  2:580407/250‑1 (MQ=255)                                                                                 gcgcAGCAAAAATTTCTGGCGAAAACTACGGCGAGGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGt                                                                                                                                                           <  1:182083/250‑1 (MQ=255)                                                                                  cgcAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGtt                                                                                                                                                          <  2:380059/250‑1 (MQ=255)                                                                                            aTTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATg                                                                                                                                                <  2:166826/250‑1 (MQ=255)                                                                                             tttCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGa                                                                                                                                               <  1:128204/250‑1 (MQ=255)                                                                                                   gCGAAAACTACGGCGATGCCAGAATGTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCg                                                                                                                                           <  2:461269/248‑1 (MQ=255)                                                                                                      aaaaCTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTg                                                                                                                                      <  2:615975/250‑1 (MQ=255)                                                                                                       aaaCTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGACAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTgg                                                                                                                                     <  1:147549/250‑1 (MQ=255)                                                                                                             cGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCatat                                                                                                                                <  1:420989/249‑1 (MQ=255)                                                                                                               gCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGa                                                                                                                             <  1:61700/250‑1 (MQ=255)                                                                                                                cGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAg                                                                                                                            <  1:499671/250‑1 (MQ=255)                                                                                                                 gATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGt                                                                                                                           <  2:442115/250‑1 (MQ=255)                                                                                                                  aTGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTa                                                                                                                          <  1:372066/250‑1 (MQ=255)                                                                                                                     ccAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTAtt                                                                                                                        <  1:522347/249‑1 (MQ=255)                                                                                                                       aGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTAtt                                                                                                                        >  1:69659/1‑247 (MQ=255)                                                                                                                        gAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAg                                                                                                                     >  1:213294/1‑249 (MQ=255)                                                                                                                          aTTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGaa                                                                                                                   >  1:573148/1‑249 (MQ=255)                                                                                                                             tACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAAtt                                                                                                               >  2:565025/1‑250 (MQ=255)                                                                                                                              aCCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCTGAAAAttt                                                                                                              >  2:164457/1‑250 (MQ=255)                                                                                                                               cccGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTa                                                                                                             <  2:296611/250‑1 (MQ=255)                                                                                                                                             tCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCATCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGaa                                                                                                <  1:144251/249‑1 (MQ=255)                                                                                                                                             tCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGa                                                                                                 <  2:332816/248‑1 (MQ=255)                                                                                                                                                  gTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGt                                                                                          >  1:285751/1‑250 (MQ=255)                                                                                                                                                       aGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGCTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGACAATTTATGAAAAAGCGGAAAACCGTgaag                                                                                      >  2:478172/1‑249 (MQ=255)                                                                                                                                                         aCTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGaagaag                                                                                   <  1:506308/250‑1 (MQ=255)                                                                                                                                                              gAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGcgtta                                                                              >  1:284516/1‑250 (MQ=255)                                                                                                                                                                  aaGTGACAGGCTGCTGATAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGcgttacgtt                                                                          <  1:236975/250‑1 (MQ=255)                                                                                                                                                                         aGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTgcgc                                                                   >  1:120743/1‑250 (MQ=255)                                                                                                                                                                            ctgctgAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAATTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTgg                                                                <  2:557985/250‑1 (MQ=255)                                                                                                                                                                               ctgAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGa                                                             <  1:172756/250‑1 (MQ=255)                                                                                                                                                                                       ttCGGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATc                                                     <  1:418482/250‑1 (MQ=255)                                                                                                                                                                                         cAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGc                                                   >  1:607068/1‑250 (MQ=255)                                                                                                                                                                                           gATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCg                                                 <  2:285751/250‑1 (MQ=255)                                                                                                                                                                                              ggCGTAAAGGTCCGTTCAGTTAGCTGCTACAT‑TTCATGGAATCAATGGTTATA‑ATGGCGTCGATTTCACCAATGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGGTGAAGTTAATGACCCGGATTGGCATATGGATTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGAc                                              <  2:118716/250‑1 (MQ=255)                                                                                                                                                                                              ggCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGAc                                              >  1:234303/1‑250 (MQ=255)                                                                                                                                                                                              ggCGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGAc                                              >  2:594570/1‑250 (MQ=255)                                                                                                                                                                                               gCGTAAAGGTCCGATCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACt                                             <  2:262324/250‑1 (MQ=255)                                                                                                                                                                                                cGTAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACt                                             >  1:215564/1‑249 (MQ=255)                                                                                                                                                                                                  tAAAGGTCCGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGAt                                          <  2:445125/250‑1 (MQ=255)                                                                                                                                                                                                          cGTTCAGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTACGTTAt                                  <  1:525100/250‑1 (MQ=255)                                                                                                                                                                                                               aGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTACGTTATGgaga                             <  1:631888/250‑1 (MQ=255)                                                                                                                                                                                                               aGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTACGTTATGgag                              <  1:823/249‑1 (MQ=255)                                                                                                                                                                                                               aGTTAGCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTACGTTATGgag                              >  1:29566/1‑249 (MQ=255)                                                                                                                                                                                                                    gCTGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTGTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACACGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTACGTTATGGAGAATTc                         <  1:404181/249‑1 (MQ=255)                                                                                                                                                                                                                      tGCGACAT‑TTCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTACGTTATGGAGAATTCtt                       >  2:564469/1‑249 (MQ=255)                                                                                                                                                                                                                               ttCATGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTACGTTATGGAGAATTCTTAACGCAccc              >  1:574097/1‑250 (MQ=255)                                                                                                                                                                                                                                  aTGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTGCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTACGTTATGGAGAATTCTTAACGCACCCGt            <  2:428940/249‑1 (MQ=255)                                                                                                                                                                                                                                   tGAAATCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTATGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTACGTTATGGAGAATTCTTAACGCACCCGTGc          >  2:296664/1‑250 (MQ=255)                                                                                                                                                                                                                                      aaTCAATGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTACGTTATGGAGAATTCTTAACGCACCCGTGCCGa       >  1:299177/1‑250 (MQ=255)                                                                                                                                                                                                                                            tGGTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTACGTTATGGAGAATTCTTAACGCACCCGTGCCGACTTAt  >  1:490749/1‑249 (MQ=255)                                                                                                                                                                                                                                             ggTTATA‑ATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTACGTTATGGAGAATTCTTAACGCACCCGTGCCGACTTAt  >  1:206818/1‑248 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  CGCGTTAACAATACATTGAGATTAGGCCAGTGGGAAAGTTCGTTCATCCACTCAACGCTTAAACGCGGCATAATCAACGAGCGCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGATGGCGTAAAGGTCCGTTCAGTTAGCTGCGACATATTCATGAAATCAATGGTTATACATGGCGTCGATTTCACCATTGCGTATCTTAACCAAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCATATGGAGTATTCAGAAAATTTATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTACGTTATGGAGAATTCTTAACGCACCCGTGCCGACTTAT  >  NZ_CP009273/914314‑914798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑