Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	748,581	0	T	A	40.8%	59.3 / 27.6	49	intergenic (‑330/+60)	ybgD/gltA	fimbrial protein/citrate synthase
Reads supporting (aligned to +/- strand):  ref base T (23/6);  new base A (0/20);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.63e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTTAAAAATATTGTTAGTTTTATTAAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGAC‑TACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAG  >  NZ_CP009273/748333‑748830                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                          cTTAAAAATATTGTTAGTTTTATTAAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGAC‑TACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTA‑TTTTACAATTTTTTGTGTTTAGGCAtata                                                                                                                                                                                                                                                          >  2:404875/1‑249 (MQ=255)    aaaaaTATTGTTAGTTTTATTAAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGAC‑TACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATaa                                                                                                                                                                                                                                                         <  1:345214/248‑1 (MQ=255)           ttGTTAGTTTTATTAAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGAC‑TACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAcc                                                                                                                                                                                                                                                >  2:54958/1‑250 (MQ=255)            tGTTAGTTTTATTAAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGAC‑TACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAccc                                                                                                                                                                                                                                               >  2:223000/1‑250 (MQ=255)                    ttattaAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGAC‑TACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAGCCCGCCatat                                                                                                                                                                                                                                        <  1:93769/249‑1 (MQ=255)                     tattaAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGAC‑TACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATg                                                                                                                                                                                                                                       >  2:343024/1‑249 (MQ=255)                     tattaAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGAC‑TACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATg                                                                                                                                                                                                                                       >  2:185035/1‑249 (MQ=255)                            tGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGAC‑TACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAAcggcgg                                                                                                                                                                                                                               >  1:57644/1‑250 (MQ=255)                               aaaaCTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTAATAAATGCGAC‑TACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAACCGCCATATGAACGGCGGGt                                                                                                                                                                                                                             >  1:496615/1‑249 (MQ=255)                                aaaaaaaaTTATTGGTATCATAAATTTGTTGTATGAAGAAAAAAATAGGGGGGGGTATAAATAGACATCATTTTCATAGGTTTAAAAGTGGGAT‑TACCATAAAGTTTTAAGTTAAAAGTGTTGGGTTGTTAATAATTTGGGCTGTTCTATTCTTTATAAATATCGATAAGGGGCTGTTAAGGATTTTTATTTTTAAATTTTTTTGTGTTAAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTa                                                                                                                                                                                                                           <  1:199763/245‑1 (MQ=255)                                   ttAAATTTTTGGTATCATAATTTTGTTTTATGATAAATAAAATATGGGGGGGTATAGATAGACGTTATTTTCATAGGGTTATAAATGCGAC‑TACCAGGAATTTTTTAATTAAAAGTATTGGGTTGCTAATATTTTGAGTTTTTTTATTTTTTTAAAATTTCTATATAGGTCTTTAAATGGTTTTTTTTTTTACATTTTTTTGTGTTAAGACATAAAAAAATCAACCCGCCATATGAACGGCGGGTTaaaa                                                                                                                                                                                                                        <  2:8852/249‑1 (MQ=255)                                          attGGTATAATAAATTTGTTTTATGAAAAATAAAATAGAGGTGGGTATAAATAGACTTAATTTTCATAGGTTTATAAATG‑GACTTACAATGAAGTTTTTATTTGAAATTTTTGGGTTGTAGATAATTTGACTTGTTCTTTTCTTTTAAAATATTTATATGGGTTTGTTAATGAATTTTATTTTTAAATTTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTa                                                                                                                                                                                                                  <  1:604344/249‑1 (MQ=255)                                            tgttATCATAAATTTGTTTTATGATAAAAAAAATATAGGGGGGTATAGATATACGTCTTTTTCATAGGTTTATAAATGCAAC‑TCCCATGATTTTTTTAATTAAAAGTATTGGTTTGTTTATATTTTGATTTTTTTTTTTTTTTTAAAATATTTTTATAGTTTTTTTAATGTTTTTTTTTTTTACATTTTTTTGTTTTTGCAAATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACa                                                                                                                                                                                                                <  2:652078/246‑1 (MQ=255)                                              ttATCATGAATTTTTTGTATGATAAAAAAAATATGGGGGGTTATAAATAGACGTCTTTTTAATGGGGTAATAAATGCAAT‑TACCATAAATTTTTTAATTGAAAGTATGGGGTTGCTGATAATTTGAGCTGTTCTATTTTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACATTTTTTTGTGTTTAGGAATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAAc                                                                                                                                                                                                              <  1:359184/248‑1 (MQ=255)                                                 tCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGAC‑TACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAg                                                                                                                                                                                                          >  1:316092/1‑250 (MQ=255)                                                                        tAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGAC‑TACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAAGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAAAATTTACAATTTAGCAATCAACCATTAAAGCCTGata                                                                                                                                                                                   >  1:452340/1‑250 (MQ=255)                                                                          aaaTATAGGGGGGTATAGATATACGTCTTTTACATGTGTTTATAAATGCGAC‑TCCTATAAATTTTTATATTGAAATTATTGTGTTGGTTTTTATTTGGTCTTTTCTTTTTTTTTAAAATATTTATAGAGTTCTTTTAATGGTTTTTATTTTAACATTTTTTTTTTTTTAGGAATAAAAAAACCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATc                                                                                                                                                                                 <  2:338497/250‑1 (MQ=255)                                                                                aGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGAC‑TACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGGTTAAATATTTAAAAATTTACAATCAACAAATAACGCTTGATATCGCtttt                                                                                                                                                                           >  2:125811/1‑250 (MQ=255)                                                                                       tataGATAGACGTCATTTTCATAGGGTTATAAATGCGAC‑TACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAAACATTAACGCTTGATATTGCTTTTAAAGCcg                                                                                                                                                                    >  1:36444/1‑250 (MQ=255)                                                                                             aaaacggtttttaCATAGGTTTAAAAATGCGAC‑TACCATAAATTTTTAAATTGAAAGTATGGGGTTGCTGATATTTTGAGCTTTTTTTTTTTTTTTAAATATTAAAAAAGGGTTGTAAATGGATTTTATTTTTAAAATTTTTTGTGTTAAGGAAAAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGtttt                                                                                                                                                              <  2:171482/241‑1 (MQ=255)                                                                                               gaCGTTTTTTTTATAGGGTTATAAAGGCGAC‑TACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTAATAATTTGATCTGTTTTTTTCTTTTTAAATATCTATATAGGTTTGTTAATGGTTTTTATTTTTACAATTTTTTGTGTTTAGGAATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGttttt                                                                                                                                                             <  2:89399/249‑1 (MQ=255)                                                                                                acgtatttttTATGGGGTTAAAAAGGCTAT‑TACTATGATTTTTTTAATTGAAAGTTTTGGTTTGTTGATAATTTGGGTTTTTTTTTTTTTTTAAAATATCTATATGGTTTTTTTAAGGTTTTTTTTTTTTAAATTTTTTTGTTTTTAGCAAAAAAAAAACAAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCa                                                                                                                                                           <  2:553028/244‑1 (MQ=255)                                                                                                          cATGGGTTTATAAATGCGCT‑TCCCATGAATTTTTTAATTAAATGTTTGGGTTTGTTAATATTTTGAGTTGTTTTTTTTTTTTTAAATATTTATATGGGTCTGTTAATGGTTTTTTTTTTTACATTTTTTTGTGTTTAGGCATAAAAAAATAAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGta                                                                                                                                                  <  1:232/249‑1 (MQ=255)                                                                                                                      aaggagAC‑TACAATGAATTTTTTAATTAAAAGTTTTGGTTTGTTGATAATTTGAGCTTTTTTATTTTTTTAAAATATCTAAATAGGTTTGTTAATGGATTTTATTTTTACATTTTTTTGTGTTTAGGAATAAAAAAATAAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGAcgcg                                                                                                                                     <  1:532906/245‑1 (MQ=255)                                                                                                                      aaTGCGAC‑TACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGAcgcg                                                                                                                                     >  1:106657/1‑250 (MQ=255)                                                                                                                         gCGAC‑TACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTTAAACTTTGAAATCAACCATTAAAACTTGGTATCGCCTTTTAAATCGCGTTTTTTCTATCTTGTATACAGCTGACGCGGAc                                                                                                                                  >  2:49464/1‑250 (MQ=255)                                                                                                                            aC‑TACCATGAATTTTTTAATTGAAATTATTGGTTTGCTGATATTTTGAGTTGTTCTTTTTTTTTAAAATATCTATATAGGTTTTTAAATGGATTTTATTTTTAAATTTTTTTGTGTTTAGGAATAAAAAAACCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACggg                                                                                                                               <  1:44490/250‑1 (MQ=255)                                                                                                                                      aaGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGCTCTATTCTTTTTAAATATCTCTATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGTTTAAAATATTTATAACTTCTAAATCAACAATTAATGACTGATAACACTTTTTATATCGCGTTTTTTATATCATGTTTCAAGATACCGCGGACAGGCAAtacttt                                                                                                                      >  2:402918/1‑245 (MQ=255)                                                                                                                                                   aatgttttGGTTTGTTGATAATTTGAGCTTTTTTTTTCTTTTTAATTATCTATATAGGTTTGATAATGGATTTTATTTTTACATTTTTTTTTGTTTAGAAAAATAAAAATCATCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACtgt                                                                                                          <  1:10539/243‑1 (MQ=255)                                                                                                                                                        aTTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAATGGCGGGGTATAATATTTATAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTACATTCGCGTTTTTCCTATACTGTATTCAACTGACGCGGCCGGGGAATCTTCATACTGTCACTGGGTAttt                                                                                                    >  1:495415/1‑250 (MQ=255)                                                                                                                                                            ggTTGCTGATAATTTGGTCTTTTTTATTTTTTTTAAATATATATATAGGTTTGTTAATTGATTTTTTTTTTAAATTTTTTTGTTTTTAGAAAAAAAAAAACCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCt                                                                                                <  2:285486/250‑1 (MQ=255)                                                                                                                                                              ttGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTTAAATATTTTAAAATTTAAAATTAACCATTAACTCTTGATTTCGCTTTTTAAGACTCGTTTTTCATATCCTTTATACAGCTTAAGCGGACGGGCAATCTTTATTACGTCAATGTGCCTTTCGATcc                                                                                              >  2:648993/1‑250 (MQ=255)                                                                                                                                                                    atatTTTGAGTTTTTTTTTTTTTTTAAAATTTTTATATAGTTTTTTAAATTTATTTTTTTTTTAAATTTTTTTTTTTTTAGGAATAAAAAAACCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTggg                                                                                        <  1:393564/250‑1 (MQ=255)                                                                                                                                                                       aTTTGATCTGTTTTATTTTTTTTAAATTTTTATATGGTTCTTTTAATGGATTTTTTTTTTACATTTTTTTGTGTTTAGGCATATAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCg                                                                                      <  1:343024/249‑1 (MQ=255)                                                                                                                                                                         ttGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTTAAATATTTTCAACTTATCAATCAACCATTAACGCCTGATTTCGCTTTTTAATTCGCGTTTTTCATATCCCGTATACAGCTGAACCGTCCGGCCAATCTTCATACCGTCACTGAGTATTTTGCCCCATTGGGGGATc                                                                                   >  2:352064/1‑250 (MQ=255)                                                                                                                                                                           gAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATc                                                                                   >  1:358419/1‑248 (MQ=255)                                                                                                                                                                               tGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTAAAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCCGcc                                                                              >  1:344615/1‑249 (MQ=255)                                                                                                                                                                                        tttttaAAATATATATAGAGGTTTGTAAAGGTTTTTTTTTTTTAAATTTTTTTGTTTTTAGAAAAAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACg                                                                     <  2:414792/248‑1 (MQ=255)                                                                                                                                                                                                   tttatataGGTTTGTTATTGGTTTTTATTTTTACATTTTTTTGTGTTTAGAAATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGaa                                                         <  1:38805/248‑1 (MQ=255)                                                                                                                                                                                                    atatataGGTTTTTTAATGGTTTTTATTTTTAAAATTTTTTTTGTTTAGGAATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGaa                                                         <  1:132651/248‑1 (MQ=255)                                                                                                                                                                                                    atatataGGTTTTTTAATGGTTTTTATTTTTAAAATTTTTTGTGTTTAGGAATAAAAAAACCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGaa                                                         <  1:393214/248‑1 (MQ=255)                                                                                                                                                                                                     tatataGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTTGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGaaaa                                                       >  1:154829/1‑250 (MQ=255)                                                                                                                                                                                                      atataGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTTCAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAATGTCGCGTTTTTCATATTCTGTATACAACTGACGCGGACGGGCAATTTTCATACCCTCACCGTGCATTTCGCTCCAGTGGGCGATCCATCCAACGGTACGTGCCACTGCGACAAt                                                      >  1:554371/1‑250 (MQ=255)                                                                                                                                                                                                                tttAATGTTTTTTATTTTTACAATTTTTGTTGTTAAGAAATAAAAAAACCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGaa                                             <  2:622020/248‑1 (MQ=255)                                                                                                                                                                                                                       gATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTTATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCCTACCCTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATgg                                        >  1:426001/1‑247 (MQ=255)                                                                                                                                                                                                                       gATTTTATTTTTACAATTTTTTGGGTTTAGGCATATAAAAACCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAg                                     <  1:436174/250‑1 (MQ=255)                                                                                                                                                                                                                                     atttttttttGTTTAGACAAAAAAAAACCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATc                       <  1:125811/248‑1 (MQ=255)                                                                                                                                                                                                                                     atttttttGTGTTTAGGAATAAAAAAACAACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATc                       <  2:94245/248‑1 (MQ=255)                                                                                                                                                                                                                                       ttttttGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTTAAATATTTTCAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTTCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGTCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCCTCGc                     >  2:146836/1‑250 (MQ=255)                                                                                                                                                                                                                                                      cATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCCACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGGTGATAc       >  1:490637/1‑249 (MQ=255)                                                                                                                                                                                                                                                      aataaAAAAAACAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATAcc      <  1:402717/249‑1 (MQ=255)                                                                                                                                                                                                                                                          taAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAAATTAACAATCAACCATTAAAGCCTGATATCGCTTTTAAAGTCGCGTTTTTTATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATTCCCTTACTGTGCATTTTGCTCCAATGGGCGGTCCAGCCAACGGGACGTGCCATTGCCAAAATGACGGGGAACATGGAAGACGGAATACCCCACGCTTTCCGGCTGACTCCagag  >  2:77530/1‑250 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                          CTTAAAAATATTGTTAGTTTTATTAAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGAC‑TACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAG  >  NZ_CP009273/748333‑748830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑