Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,199,799	0	C	A	34.0%	70.4 / 18.6	54	intergenic (‑184/‑23)	ttdR/ttdA	DNA‑binding transcriptional activator TtdR/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (13/22);  new base A (5/13);  total (19/35)
Fisher's exact test for biased strand distribution p-value = 5.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATA‑TTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAG‑TGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCG‑CCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAG  >  NZ_CP009273/3199555‑3200037                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   cGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATA‑TTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATa                                                                                                                                                                                                                                               <  2:296259/250‑1 (MQ=255)    tgtgAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATA‑TTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑GATTTTTTCTAACAAAAACa                                                                                                                                                                                                                                             >  1:27774/1‑249 (MQ=255)         aCGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATA‑TTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGA‑G‑AATTTTTCCCGACAAATACAAAAACt                                                                                                                                                                                                                                       >  1:616719/1‑250 (MQ=255)           gATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATA‑TTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTgg                                                                                                                                                                                                                                     <  1:534877/250‑1 (MQ=255)             tttCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATA‑TTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                                                                                                                                   <  2:397247/250‑1 (MQ=255)              ttCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATCGCCATTCCCTGCTCTGGCGCAATA‑TTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑TATTTTTTCACAAAAAAACAAAAACTGGaat                                                                                                                                                                                                                                  >  1:247508/1‑248 (MQ=255)                ctagcaggtccTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGGTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCTATA‑TTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGtt                                                                                                                                                                                                                                 <  2:642959/240‑1 (MQ=255)                cTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATA‑TTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑TATTTTTTTTGACAAAAACAAAAACAGTAGTTg                                                                                                                                                                                                                                >  1:412821/1‑250 (MQ=255)                         ccTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATA‑TTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatgatg                                                                                                                                                                                                                        <  2:178741/249‑1 (MQ=255)                             cAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATA‑TTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCg                                                                                                                                                                                                                   <  1:413496/249‑1 (MQ=255)                                     tGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATA‑TTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGta                                                                                                                                                                                                             <  2:104602/247‑1 (MQ=255)                                       gCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATA‑TTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGtaataa                                                                                                                                                                                                         <  2:562291/249‑1 (MQ=255)                                              gCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATA‑TTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTTCAGACAAATACAAAAAATGGAGTTGGCATGATTA‑GCGAAAGAAATAAACAACAg                                                                                                                                                                                                  >  1:326281/1‑249 (MQ=255)                                               ccAGCTAGTCAGCATCGTTAATTATCCGCAGTCGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCGCTGGCGCAATA‑TTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGc                                                                                                                                                                                                <  2:608374/250‑1 (MQ=255)                                                 aGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATA‑TTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAg                                                                                                                                                                                              <  1:172776/250‑1 (MQ=255)                                                             tCGTTAATTATCCGAAGTTGTGACAAGCGCAGTGTATGTAGCAAAACATTGCCATTCCCTGCTATGACGCAATA‑TTCATTCTTATTTTAGCGTTGATTCACGAACTGTTAATAATATATTCACGGTGGAAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTAGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAATGTAATAAGCAACAGGCAG‑TGAATAAGTTGa                                                                                                                                                                                 <  2:267678/250‑1 (MQ=255)                                                                      aTCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATA‑TTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑GAATTTTTTAAACACATAAAAAAACAGGAGTTGTCCTGTTTA‑GGGGAAGAAATAAAAAACAGACAA‑AGAATAAATTGACagaacatg                                                                                                                                                                        >  2:447097/1‑245 (MQ=255)                                                                                    tAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCTCAATA‑TTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAG‑TGAATAAGTTGACAGAGATTGTCGCTAACTTT‑Acc                                                                                                                                                         <  2:519859/250‑1 (MQ=255)                                                                                               tATTTCGCAAAACTTTGCCTTTCCCTGCTCTGGCGATATT‑TTCATTTTTATTTTCGCTTTGATTCACGAACTTTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCAAAAGTAATAAGCAACAGGCAG‑TAAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCG‑CCATGAttt                                                                                                                                              <  2:467126/249‑1 (MQ=255)                                                                                                 tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATA‑TTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑GAAATTTTTCCAGA‑AAAAAAAAAAAAAGGGGGTGGCATGATGA‑GGGGAAGAAAAAAAAAAAAAGAAG‑GGAAAAAATTGAAAGAGAGTGGCGCTAAATTT‑ATAC‑CCATGAttttt                                                                                                                                            >  1:217712/1‑247 (MQ=255)                                                                                                   tgaaaaacCATTGCCATCCCCGGCTGTGGCG‑AATATTTCATTTTTTTTTTCGGGTTGATTCACAAACTGTTAATTAAATATTCAGGGTGAAAAAAAATAAAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑A‑AATTTTTCCAGAAAAATAAAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAG‑TGAAAAAGTTGAAAGAGATGGTCGCTAACTTT‑ACCG‑CCATGATTTCTAc                                                                                                                                          <  2:188088/245‑1 (MQ=255)                                                                                                            tttGCCATCCCTTGTCCTGGCACAATA‑TTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCAGGGTGGAAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAG‑TAAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCG‑CCATGATTTCTACCCGAATGCCt                                                                                                                                <  2:138277/249‑1 (MQ=255)                                                                                                                ccATTCCCTGCTCTGGCGCAATA‑TTCATTCTTATTTTCTCGTTGATTCACGAACTTTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGGGGCGGT‑A‑AATTTTTCCAGAAAAATACAAAACCTGGAGTTGCCTTAATGA‑GCGAAAGTAAAAAGCACCAGGCAG‑TGAATAAGTTGACAGAGATTGTCGCTAACTTA‑ACCG‑CCATGATTTCTACCCGAATGCCtgatg                                                                                                                            <  1:90434/250‑1 (MQ=255)                                                                                                                        tGCTCTGGCGCAATA‑TTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTTCCGACAAAAAAAAAAACTAGAGTTGCCATGATGA‑GGGAAAGAAATAAACAACAAGCAG‑AGAATAAAATGAAAGAAAATGTTGCTAAATTT‑ACCG‑CCCTGATTTTTACCCGAATTCCCGATTACgtggtg                                                                                                                    >  1:193124/1‑250 (MQ=255)                                                                                                                          ctctGGCGCAATA‑TTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTTTAAAAAAATACAAAAAAAGGGGTTTCCATGGTGG‑GGGAAAGGAATAAGAAAAAAACAA‑TGGAAAAGTTAGGAGAGAATGTCGGTAACTTT‑ATCGCCCTTGTTTTTTACCCAAATACCttttggtgggggg                                                                                                                    >  2:522448/1‑237 (MQ=255)                                                                                                                             tGGCGAAATA‑TTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAG‑TGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCG‑CCATGATTTCTACCCGAATGCCTGATGACGTGGTGGAt                                                                                                                 <  1:175540/248‑1 (MQ=255)                                                                                                                               gcgcAATA‑TTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGAAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAG‑TGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCG‑CCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa                                                                                                               <  1:595268/248‑1 (MQ=255)                                                                                                                                         tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTTCAGACAAAAAAAAAAACTGGAGGTTTCATGATGA‑GCGGAAATAATAAACAACAAGCAG‑TGAATAAAGTGACAGAGATTGTCGCTAACTTT‑TACG‑CCATGATTTTTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGc                                                                                                    >  1:545127/1‑250 (MQ=255)                                                                                                                                              tttATTTTCGATTTTATTCAAGAACTGTTAATTACATATTCGCGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGATA‑GTTTGTGGCGGT‑G‑AATTTTTCCAGAAAAAAACAAAAACGGGAGTTGCAATGATGA‑GCGAAAGAAATAAAAAACAGGCAG‑TGAATAAGTAGACAGAATTTGTCGCTAACTTA‑ACCG‑CCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaaa                                                                                                <  2:553749/248‑1 (MQ=255)                                                                                                                                                 aTTTTCGCGTTGATTCACGAACTTTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAG‑TGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCG‑CCATGATTTCTACCCGAATGCTTGATGACGTGGTGGATAAACTAAAACAGCTAAAg                                                                                               <  1:410853/247‑1 (MQ=255)                                                                                                                                                  ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTTCAAGAAAATAAAAAAAATGGAGTTGGCAGGAGGA‑GGGAAAAAAAAAAAAAAAAAGCAG‑GGGAAAAGATGGAAAAGAATGGTGGTAAATTT‑TTCG‑CCATGAATTTTAACCGAAAGGCGTATGGCGGGGGGGGTGAACTAAAAAAgacaaaagaag                                                                                           >  2:454499/1‑240 (MQ=255)                                                                                                                                                       gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AAATTTTTCAGACAAAAACAAAAAATGGAGGTGCCATGATGA‑GCGAAAGAAAAAAACAACAGGGAG‑TGAATAAATTGACAGAGATTGTCGCTAAATTT‑ACCC‑CCCTGATTTTTACCCCAAAGACTGGTGACGGGGGGGGTAAAATAAAAAAGCTAAAAGAAGCCGa                                                                                       >  1:512105/1‑249 (MQ=255)                                                                                                                                                               tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGTGG‑AATTTTTCAAAAAAAAAAAAAAAACT‑GAGTTGGCATGATGAGGGGAAAGAAAAAACAAAAAGCAAG‑GGAAAAATTTG‑CAGAGAATGTTGCTAAACTT‑ACCG‑CCATGATTTCTTACCCAAAGCCTGGAGACGTGGGGGATAAAATCAAAAAGACAAAAAAAGCCGAAAcatcggc                                                                              >  2:463924/1‑248 (MQ=255)                                                                                                                                                               tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTTCAGACAAAAAAAAAAAAAGGAGGTGGCATGATGA‑GCGGAAATAAAAAAAAAAAAGCAG‑AGAATAAAATGAAAGAGAATGTTGCTAACTTT‑ACCG‑CCCTGATTTCTATCCGAAAGCCTGATGACGTGGGGGATAAAATAAAAAAACTAAAAGAAGCCGGAACgtcgtc                                                                              >  2:598738/1‑250 (MQ=255)                                                                                                                                                                    aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTTCCGACAAAAACAAAAACTGGAGGTGGCATGATGA‑GGGAAAATAATAAACAAAAAGGAG‑TGAATAAAGTGAAAGAGATTGTCGCTAACTTT‑ACCG‑CCCTGATTTCTACCCGCATGCCTGATGACGTGGGGGGTAAAATAAAAAAGCTAAAAGATGACGAAACGTCGTCGGTgg                                                                         >  1:435118/1‑250 (MQ=255)                                                                                                                                                                             ttACATATTCAGGGTGGCAAAAAATAAAAAACCAA‑TTTTGGAGGGGAT‑GTTTGGGGTGGT‑G‑AATTTTTCCAAACAAATACAAAAGCGGGAGTGGCCATGAGGG‑GCGAAAGTAATAACCAACAGGCAG‑TGAATAAGTTAACAGGGATTGTCGCTAATTTT‑ACCG‑CCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTa                                                                <  2:57271/250‑1 (MQ=255)                                                                                                                                                                                     tCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGTGG‑AATTTTTCC‑GAAAAATACAAAAAATGGGGGTTCCATAATGA‑GCGGAAATAATAAACAAAAAGGAG‑TGAAAAATTTGACAAAGATTGTCGCTAACTTT‑ACCG‑CCATGATTTTTACCCGAAATCCTGATGACGTGGTGGATAAAATAAAACAAATAAAAGATGCCCAAACGTCGTCGATTGGGGAAAATATTTACCa                                                         >  1:213716/1‑249 (MQ=255)                                                                                                                                                                                      cagggTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGGGGT‑G‑AATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAG‑TGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCG‑CCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCAt                                                        <  1:403793/246‑1 (MQ=255)                                                                                                                                                                                      cACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTTCAGACAAATACAAAAAATGGAGATTCCATTATGA‑AGGAAAGGAATAAAAAAAAGGCAG‑TGAATAAATTGAAAGAGATTGTCGCTAAATTT‑ACCC‑CCATGATTTTTACCCCAAAGCCCGATGACGTGGGGGGAAAAATAAAACAACTAAAAGAAGCCGAAAACGCGTCGGAGGGGGAAAATAACTAACAt                                                        >  1:375116/1‑249 (MQ=255)                                                                                                                                                                                            gggaaaaaaaaaaaaaaCACAATTTTTAGGGGTA‑TTTTGGGGGGGG‑A‑AATTTTCCAAAAAAAAAACAAAAACGGGAGTTCCCAGGATGA‑GCAAAAGAAAAAAGCAACAGGCAG‑TAAATAAGTGGAAAGAGATTGTCGCTACTTTT‑ACCG‑CCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATg                                                  <  2:545127/247‑1 (MQ=255)                                                                                                                                                                                             gaaaaaaaaaaaaaACCAT‑AGTTTAAG‑GGTATGTTTGGGGGAGT‑A‑AATTTTACAAAAAAAATAAAAAAACGGGAGTTGCAGTGATGA‑GCGAAAGTAATAAGCAACAGGCAG‑TGAATAAGTTGAAAGAGATTGTCGCAAATTTT‑ACCG‑CCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGtt                                                <  2:438215/248‑1 (MQ=255)                                                                                                                                                                                               aaaaaaTATAAAACCAT‑ATTTTAATGTGTA‑TTTGGGGGGGGA‑A‑TATTTATCAAGAAAAAAACAAAACCTGGAGTTGCCGTGAGGA‑AAGAAAGAAATAAGCAACATGCGG‑TAAATAATATAACAGAGATTGCGGATAATTTA‑ACCG‑CCATGATTTCTAACCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCg                                              <  2:591010/250‑1 (MQ=255)                                                                                                                                                                                                 aaaaTAAAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTCCAGAAAAATACAAAAACGGGAGTTGCCATGATGA‑GCGAAAGAAAAAAGCAACAGGCAG‑TGAATAAGTTGACAGAGATTGTCGATAACTTT‑ACCG‑CCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGac                                            <  1:598365/250‑1 (MQ=255)                                                                                                                                                                                                          aaCCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTCCCAAAAAAATAAAAAAACGGGATTTGCCATGATGA‑GCGAAAGTAAAAAGCAACAGGCAG‑TGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCG‑CCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCaa                                   <  1:81725/250‑1 (MQ=255)                                                                                                                                                                                                           cccAT‑ATTTTTAGTGGTA‑TTTTGGGGGGTT‑A‑AATTTTTCCAGACAAAAACAAAAACGGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAG‑TGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCG‑CCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCaaa                                  <  1:508577/249‑1 (MQ=255)                                                                                                                                                                                                              ac‑aTTTTTATGGTTA‑GTTTGTGGCGGT‑A‑AATTTTTCCAGAAAAAAACAAAACCTGGAGTTGACATGATGA‑GGAAAAGTAATAAGCAACAGGAAG‑TGAATAAGTTCACAGAGATTGTCCCTAACTTT‑CCCG‑CCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAg                               <  1:458257/250‑1 (MQ=255)                                                                                                                                                                                                               c‑aTTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAG‑TGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCG‑CCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGc                              <  1:227093/250‑1 (MQ=255)                                                                                                                                                                                                                 aTTTTTAGTGGTA‑GTGTGGGGCGGT‑G‑AATTTTTCCAGAAAAATACAAAAACGGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAG‑TGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCG‑CCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCg                             <  2:512105/250‑1 (MQ=255)                                                                                                                                                                                                                              ggTTTGTGGGTGG‑A‑AATTTTACCAGCAAAATAAAAAAGCGGGGGTGACCATGAGAA‑GCGAAAGAAATAAGCAGC‑GGCGGTTAAATAGGTTAAGAAATGTTGTC‑ATATGTTTAACCG‑CCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGAGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAAt                 <  2:132036/249‑1 (MQ=255)                                                                                                                                                                                                                                 ttGTGGCGGT‑A‑AATTTTCCCAGAAAAATACAAAAACGGGAGTTGCCATGATGA‑GCAAAAGTAAAAAGCAACGGGCAG‑TAAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCG‑CCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCg               <  2:582587/249‑1 (MQ=255)                                                                                                                                                                                                                                    ggggggT‑G‑ATTCTTTCAAGAAAAATACAAAAACGGGAGTTGCCATGAGGA‑GCAAAAGTAATAAACAACCGGTAT‑TGAATAGGTTAACAGAAGTGGTCGCTACTTTT‑ACCG‑CCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcc            <  2:52940/245‑1 (MQ=255)                                                                                                                                                                                                                                     ggCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAG‑TGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCG‑CCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGAATGACCTGAATCGTactg          >  2:577248/1‑250 (MQ=255)                                                                                                                                                                                                                                          t‑G‑AATTTTTCAAAACAAATACAAAAACTGGAGTCGCCATGAGGG‑GAAAAAGTAAAAAGCAACAGGCAG‑GAAATAAGTTGACAGAGATGGTCGCTAATTTA‑ACCG‑CCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGt     <  2:193124/250‑1 (MQ=255)                                                                                                                                                                                                                                                tttttCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGCAATAAGCAACAGGCAG‑TGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCG‑CCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGTTGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATCCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAg  >  1:535070/1‑249 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   CGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATA‑TTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTAGTGGTA‑GTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAG‑TGAATAAGTTGACAGAGATTGTCGCTAACTTT‑ACCG‑CCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAG  >  NZ_CP009273/3199555‑3200037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑