Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,659,062	0	C	A	32.1%	109.3 / 17.3	53	A37S (GCG→TCG)	gadX	acid resistance transcriptional activator GadX
Reads supporting (aligned to +/- strand):  ref base C (33/3);  new base A (0/17);  total (33/20)
Fisher's exact test for biased strand distribution p-value = 3.53e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGTTCTACGGGCAGGA  >  NZ_CP009273/3658814‑3659306                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     aaaGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGc                                                                                                                                                                                                                                                      >  2:220385/1‑249 (MQ=255)   aGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCaa                                                                                                                                                                                                                                                    >  2:241843/1‑249 (MQ=255)   aGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCaa                                                                                                                                                                                                                                                    >  1:588280/1‑249 (MQ=255)          tGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTACATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAgg                                                                                                                                                                                                                                            >  2:210660/1‑250 (MQ=255)                 ttACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCAcctc                                                                                                                                                                                                                                     >  1:226898/1‑248 (MQ=255)                  tACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGc                                                                                                                                                                                                                                     >  2:25972/1‑249 (MQ=255)                    cGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCAt                                                                                                                                                                                                                                  >  1:32315/1‑250 (MQ=255)                    cGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCAAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCa                                                                                                                                                                                                                                   <  2:492823/249‑1 (MQ=255)                             tCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGc                                                                                                                                                                                                                         >  1:343706/1‑250 (MQ=255)                                 cATAGGAAAGATTTTTATTCTGAGATAGTTGCGCAACTTCCTAAAAAACAGGAATATCTCTACTACAGTGATGAACGAGAAAGGAAAAAATTTTTTTATGTGAATGAAAATTTAATGCCTCCTCTTTGAGAATGGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATAA‑AAATTTT‑TCAACACACTTAT‑CTCCTCAAATTTGGCTGGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGata                                                                                                                                                                                                                     <  1:486885/250‑1 (MQ=255)                                 cATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAACAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGCTTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAAACAGGTCACCGCCATTAAAATAGCGata                                                                                                                                                                                                                     >  2:525452/1‑250 (MQ=255)                                   tAGCGAATATTTTTATTCTGCGATAGTGGCGCAACTTCCTAAAAAACAGAAATATCTCTACTACAGAGATAAAAGATTAATAAAAAATTTTTTTTATGTGAAGAAAAATTTAATCCCCCCTCCTTGAGCAGGGGAAAAAATAATGAAGGCGTCCCCCTTGACACAAATAA‑AAAATTT‑CAAACCCCCTTAT‑CTCCTCAAATTTGGCTTCCACCCAAAAAACCCAGGTCACCGCCATTAAAATAGCGATAtt                                                                                                                                                                                                                   <  2:320510/250‑1 (MQ=255)                                                 aTTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATg                                                                                                                                                                                                     >  2:249039/1‑250 (MQ=255)                                                  ttCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATgg                                                                                                                                                                                                    >  2:550379/1‑250 (MQ=255)                                                        gATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                                                                                                                              >  2:367843/1‑250 (MQ=255)                                                            gTTGCGAAACTTCATAAAAAACAGGAATATCTCTACAACAGTGAGAAACGAAAAATAAAAAATTTTTTTTATGTGAATGAAGATTAAATCCCCCCTCCTGGACAATCGGAAAAAATAAGAAAGGCGTGCCCTTTAACAAAAAAAA‑AAAATTT‑ACACCACACTTAT‑CTACTAAATTTTGGCTTGCACCCGAAAAAACCAGGTAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatat                                                                                                                                                                                           <  2:523070/249‑1 (MQ=255)                                                                         ctaaaaaaCAGAAATATCTCTACTACAGTGATGAACGAGTAATGAAAAAATTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAAAAATAATGAAAGCGTGTCCCTTGACACGAATAA‑AAAATTT‑TCAACACACTTAT‑CTACTCAATTTTGTTTTGCATCCGCAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCa                                                                                                                                                                             <  1:193829/247‑1 (MQ=255)                                                                            aaaaaCAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATAc                                                                                                                                                                          >  1:130279/1‑250 (MQ=255)                                                                             aaaaCAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACg                                                                                                                                                                         >  1:20839/1‑250 (MQ=255)                                                                              aaaCAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACg                                                                                                                                                                         >  1:8835/1‑249 (MQ=255)                                                                               aaCAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGc                                                                                                                                                                        >  1:4071/1‑249 (MQ=255)                                                                               aaCAGAAATATCTCTACTAAAGTGATGAACGAGAAATAAAAAAATTTTTTTATGTGCATGAAGATTTAATGCCCCCTCCTTGAGCATCGGGAGAAAAAATAAAAGCTTGTCCCTTGACAAAAATAC‑AAATTTT‑TAAACACACTTAT‑CTCCTCGATTTTGGCTTGCACCCGAAAAACCAAGGTCACCGCCATTAAAATAGCGACATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCa                                                                                                                                                                       <  1:133170/250‑1 (MQ=255)                                                                                            aaaaaacAGGGATGAAGTAGTAAGAAAAAAATTTTTTTAGGTGAATGAAGATTAAATGCCTCCTCTTTGAGAATGGGAAGAAATAATAAAAGCGTTTCCTTTGACACAAATAA‑AAATTTC‑TAAAAACATTTAT‑CTACGCGAATTGGGCTTGCACCCAAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTc                                                                                                                                                           <  2:332340/244‑1 (MQ=255)                                                                                               ctacattattgaACGAGTAATAAAGAATCTTTTTTAGGTGAAGAAAATTTTAATGCCTCCTCTTTGAGCAGCGGGAAAAATAAGAAAAGCTTCTCCCTTGACACAAATAC‑AAATTTT‑TAAACACCCTTAC‑CTACTCGATTTTGGCTTGCACCCAAAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCa                                                                                                                                                        <  2:542867/240‑1 (MQ=255)                                                                                                         gaACGAATAATGAAAAAATTTTTTTATGTGCATGAATATTTAATGCCTCCTCCTTGAGCAAGGGGAAAAAAAATGAAAGCGTGCCCCTTGACACAAATAA‑AAAATTT‑CCAACACCCTTAT‑CTACTCAAATTTGTCTTCCCACCCAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTg                                                                                                                                             <  1:263527/250‑1 (MQ=255)                                                                                                                 aaTGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGtt                                                                                                                                      >  1:10191/1‑249 (MQ=255)                                                                                                                         atcTTTTTATTGTCAATAAAGATTAAAGCCCTCCCCGTTGAGCATGGGGAAAAATAATGAAAGCGTTCCCCTTGACAAAAAAAA‑AAAATTT‑TCACCCCTCTTAT‑CTCCACAAATTTGGTTGGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAata                                                                                                                             <  1:342452/248‑1 (MQ=255)                                                                                                                                 tATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATa                                                                                                                      >  2:465369/1‑249 (MQ=255)                                                                                                                                  aTGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATaaa                                                                                                                    >  2:577716/1‑250 (MQ=255)                                                                                                                                             gATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGt                                                                                                         >  2:32229/1‑250 (MQ=255)                                                                                                                                              aTTTAGTCCCTCCTCCTGGAGCATCGGAAAAAAAAATAAAAGCGTGTCCCTTCACACAAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCACCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGtt                                                                                                        <  1:39523/250‑1 (MQ=255)                                                                                                                                                 tAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTttat                                                                                                     >  2:103889/1‑250 (MQ=255)                                                                                                                                                   atcccccctcctAGAGCGAGGGAAAAAAAAATAAAAGCGTTCCCCTTAACAAAAAAAA‑AAAATTT‑ACACCACATTTAT‑CTACTAAATTTTGGTTTACAACCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTttatt                                                                                                    <  2:567845/243‑1 (MQ=255)                                                                                                                                                       cccctcctTGAGCATCGGAAAAAAAAATGAAAGCGTTTCCCTTAACACGAAAAC‑AAATTTT‑TCAACCCACTTAT‑CACCTCAAATTTGCTTTGCACCCGAAAAACCCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGt                                                                                               <  1:13179/248‑1 (MQ=255)                                                                                                                                                                gAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCAcgccg                                                                                      >  1:249219/1‑250 (MQ=255)                                                                                                                                                                       gggAGAAATAATGAAGGCGTGTCCTTTAACAATAAAAC‑AAAATTT‑TCAACCCATTTAT‑CTCCTCGATTTTGGCTTCCATCCGAAAAACCAAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCtt                                                                               <  1:235606/250‑1 (MQ=255)                                                                                                                                                                       gggAGAAATAATGAAAGCGTGTCCCTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCctct                                                                                >  2:287981/1‑249 (MQ=255)                                                                                                                                                                          aaaaaTAATGAAAGCGTGCCCCTTGACACAAATAC‑AAAATTT‑CCAACACATTTAT‑CTACTCAATTTGGGCTTGCACCAGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAg                                                                            <  1:145933/248‑1 (MQ=255)                                                                                                                                                                                     aaGCGTGTCCCTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAattgatt                                                                 >  1:576713/1‑250 (MQ=255)                                                                                                                                                                                             cccTTAACAAGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCAATTTTGGCTTGCATCC‑AAAAAACCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGataaataa                                                        <  1:15416/250‑1 (MQ=255)                                                                                                                                                                                              ccTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGataaata                                                         >  1:432179/1‑249 (MQ=255)                                                                                                                                                                                               cTTTACACGAAAAAAAAAATTT‑TCATC‑CACTTTTCCTACTCAAATTTGGTTCACACCCAAAAAAACATGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGataaataa                                                        <  2:2244/250‑1 (MQ=255)                                                                                                                                                                                                   aaacaaaTAC‑AAAATTT‑AAAACACATTTTA‑CTACTGAAATTGGGATTGCACCCGAAAAAACCAGGCAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTa                                                   <  2:184160/245‑1 (MQ=255)                                                                                                                                                                                                            taaaaaTTTCTCAACACACTTAT‑CTCCCCGATTTTGGTTTGCATCCAAAAAAACCAGTTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAg                                            <  1:520240/248‑1 (MQ=255)                                                                                                                                                                                                              aaaaTTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCgg                                         >  1:59799/1‑250 (MQ=255)                                                                                                                                                                                                              aaaaTTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCgg                                         >  1:449510/1‑250 (MQ=255)                                                                                                                                                                                                                               ttAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATaa                         >  2:67301/1‑250 (MQ=255)                                                                                                                                                                                                                                tAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAAt                        >  1:207559/1‑250 (MQ=255)                                                                                                                                                                                                                                 aT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAAtg                       >  1:267848/1‑250 (MQ=255)                                                                                                                                                                                                                                     tACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAACGGGAGAACAGGATGATAAtgtgt                    >  2:468061/1‑250 (MQ=255)                                                                                                                                                                                                                                       ctctAATTTGGCTTGACCCCGCAAAACCCAGCTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAAtgtgtg                   <  1:423508/249‑1 (MQ=255)                                                                                                                                                                                                                                          gAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGt               >  1:521014/1‑250 (MQ=255)                                                                                                                                                                                                                                              ttGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGTTCt            >  2:312115/1‑249 (MQ=255)                                                                                                                                                                                                                                                       aTCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGTTCTACGGGCAGGa  >  2:83166/1‑250 (MQ=255)                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     AAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATAC‑AAAATTT‑TCAACACACTTAT‑CTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGATGATAATGTGTGATGTTCTACGGGCAGGA  >  NZ_CP009273/3658814‑3659306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑