| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,907,913 | 0 | A | G | 32.1% | 99.1 / 14.2 | 56 | H216H (CAT→CAC) | glmU | bifunctional UDP‑N‑acetylglucosamine diphosphorylase/glucosamine‑1‑phosphate N‑acetyltransferase GlmU |
| Reads supporting (aligned to +/- strand): ref base A (14/24); new base G (18/0); total (32/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.99e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTAATCACGCAACCGGTGCCAATTTTCACGCGATGACCGAGAGTCACGTTGCCCTCGATGATAACGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGC‑GTTTCATATCTGCG‑CCGTTGGCAATCAGAATGCCGG‑TGTTGATCTCCTGAATCTG‑ACGCTGCTCGT‑CGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGCCGTTTTCACGGGTGATACGTCCATAACCGGTCGGATC > NZ_CP009273/3907665‑3908158 | ttAATCACGCAACCGGTGCCAATTTTCACGCGATGACCGAGAGTCACGTTGCCCTCGATGATAACGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTCCTTCGC‑TTAAACGTTGCGGAt > 1:603057/1‑250 (MQ=255) aCGCAACCGGTGCCAATTTTCACGCGATGACCGAGAGTCACGTTGCCCTCGATGATAACGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTTTTCACGCCCTCTTCTTCGC‑TTAAACGTTGCGGATGaaacg > 1:616518/1‑247 (MQ=255) ggTGCCAATTTTCACGCGATGACCGAGAGTCACGTTGCCCTCGATGATAACGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGGTACTCACGCCCTCTTCTTCCC‑CTTAACGATGCGGGTGTACGGcgacggtg > 2:497514/1‑247 (MQ=255) ggTGCCAATATTCACGCGATGACCTGGAGACACGGTGCCATCGATGATAACGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGC‑GTAAACGTTGCGGATGAACGGCGACGAtt < 2:516242/250‑1 (MQ=255) gTGCCAATTTTCACGCGATGACCGAGAGTCACGTTGCCCTCGATGATAACGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTTACGCCTTCTACTTTGC‑TTAAACGTTGCGGGTGAACGGCGGCGGttt > 1:107927/1‑250 (MQ=255) gCCAATTGACACGCGAGGACCGTGAGTCGCTGTTCCGTCGATGAAAACGTCAGTATCAATTTCAACATCGCCCCCGTGAGTTAGCGTATCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTCACAGCAGTTTTTCAGCCTGTTCGGACGGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGC‑TTAAACGTTGCAGATGAACGGCGACGATTTCa < 2:601232/250‑1 (MQ=255) cAATTTTCACGCGATGACCGAGAGTCACGTTGCCCTCGATGATAACGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTTACGCCTTCTACTTTGC‑TTAAACGGTGGGGGTGGACGGGGGCGATTTCACg > 1:382985/1‑250 (MQ=255) aTTTTCACGCGATGACCGAGAGTCACGTTGCCCTCGATGATAACGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTTACCCCCTCTTCTTTCC‑TTTAACAGTTCGGGTGAGCGGCGCCGCGTTCaaccc > 2:204370/1‑246 (MQ=255) cACGCGATGACCGAGAGTCACGTTGCCCTCGATGATAACGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGCTTTAAACATTG‑GGGTGGAAGGGGGCGATTTTACT‑CCCCTcc > 2:349301/1‑250 (MQ=255) cgATGACCGAGAGTCACGTTGCCCTCGATGATAACGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTTACACCCTCTACTTCCC‑TTTAAAGTTGCGGGTGAACGGCGACGATTTTACG‑CCCCTCCTGa > 1:313202/1‑249 (MQ=255) gACCGAGAGTCACGTTGCCCTCGATGATAACGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCAAGCCTTCTCCTTCGC‑TTAAACGGTGCGGATGAAACGCGACGACTTTACGCCCCTTCCtataccc > 1:381597/1‑244 (MQ=255) tCGATGATAACGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTTACGCCCTCTACATCTC‑CTAAACGTTGCGGATGGACTGCGACGATTTCACC‑CCCCTCCTGACACGCCCGCGCAATAAAGTCGGGGa > 2:181724/1‑250 (MQ=255) cGATGATAACGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTTACGCCCTCTACCTCGC‑TTTAACGTTGCTGGCGAATGGCGACGCTTTCTCG‑CCCTTCCTGTTCCGCCAGCGCAATAATTTCGGTGAt > 1:15770/1‑250 (MQ=255) tgatAACGTTAGTATCAAGTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATgta < 2:187378/250‑1 (MQ=255) aCGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTTACGCCTTCTCCTTCGC‑TTTAACGTTGCGGGGGAAAGGGGGCGGTTTTACC‑CCCTTCCTGATACCCCCCCGCAAAAATGTCGGGGGTGTGGTAtt > 2:149514/1‑250 (MQ=255) gTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCg < 2:390870/250‑1 (MQ=255) cttAGTAGCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTTGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGGTGCAGGCGGTTATTCACGCCTTCTACTTCGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAAGAATGTCGGTGATGTAGTATTCg < 2:592784/249‑1 (MQ=255) aTCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGC‑TTAAACGTTGCGGGTGAACGGCGACGATTTCACG‑CCCTTCCCGTTACCCCCGCGCAATAAAGTCGGGGGTGTAGTAttttcccc > 1:223074/1‑242 (MQ=255) aTTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCat < 2:80469/250‑1 (MQ=255) aCATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACCCCTTCTACTTCGC‑TTTAAAGGTGCGGGTGAACGGCGGCGATTTCACG‑CCCCTTCTTGTAACCCAGCCCAATAATGTCGGGGGTGGAGGATTTGCCCCTGGCattatttt > 1:275817/1‑248 (MQ=255) tCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTgg < 1:376752/250‑1 (MQ=255) ccGTGAGTTAGCGTACCACGCAGATC‑AAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTTACGCCCTCTTCTTCGC‑TTAAACGTTGCGGGTGAACGGCGACGATTTCACG‑CCCCTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCTCCCCGAGCATTTTTGTTTGTTAGCtt > 1:186661/1‑250 (MQ=255) gAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCCTCTACTTCGC‑TTTAACGTTGCGGGTGAACGGGGACGATTTCACC‑CCCCTTCTGATACGCCAGCGCAATAATGTCGGGGATGTAGGATTTGCCCCCAGCCTTATTGTTTGTCTGCttttc > 2:401776/1‑247 (MQ=255) aGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTTTTCACCCCCTCTACTTCGC‑TTAAACGTTGCGGGTGAACGGCGACGATTTCAAG‑CCCTTCCTGCTACGCCAGCGCAATAATGTCGGTGGTGTAGTATTTGCCCTTAGCCTTATTTTTTGTTAGCtttccc > 1:98002/1‑246 (MQ=255) ttAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCgccag < 1:635313/250‑1 (MQ=255) gCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCgccagcc < 1:180357/249‑1 (MQ=255) aGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGC‑GTTTCatat < 1:17010/250‑1 (MQ=255) aaaaCGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGC‑GTTTCATATCTgc < 2:124430/249‑1 (MQ=255) cgcTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGC‑TTAAAAGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGGGATGTAGTATTCGCCCTGAGCATTATTGTTGGGCAGCTTCGCCAGCCAGC‑GGTTCATATCTGCG‑CCGGTg > 1:111057/1‑250 (MQ=255) gCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGC‑TTAAACGTTGCGGGTGAAAGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAAAAATGTCGGTGGTGTAGTATTTGCCCTTGGCCTTTTTTGTGGTCAGCTTCCCCAGCCAGC‑GTTTCATATCTGCG‑CCGTTGGGCATCAGAATGCCGG‑GGTTg > 1:566937/1‑250 (MQ=255) gCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGC‑GTTTCATATCTGCG‑CCGTTGGCAATCAGAATGCCGG‑TGTTg < 2:112457/250‑1 (MQ=255) cTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTTTTTACGCCTTCTACTTCGC‑TTAAACGGTGCGGGTGAACGGCGACGATTTTACG‑CCCTTCCTGGTACGCCAGCGCAATAATGTCGGGGGTGTAGTATTCGCCCCGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGC‑GGTTCATATCTGCG‑CCCGTGGGAATAAGAAAGCCGG‑TGTTGAtctc > 1:506560/1‑250 (MQ=255) aCAGCAGTTTTTCATCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGTTGATGTAGTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGC‑GTTTCATATCTGCG‑CCGTTGGCAATCAGAATGCCGG‑TGTTGATCTcc < 1:489065/248‑1 (MQ=255) cagTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGC‑GTTTCATATCTGCG‑CCGTTGGCAATCAGAATGCCGG‑TGTTGATCTCCTGAATc < 1:521330/250‑1 (MQ=255) cgtaCTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTACTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGC‑GTTTCATATCTGCG‑CCGTTGGCAATCAGAATGCCGG‑TGTTGATCTCCTGAATCTG‑ACGCTGCTCGT‑Cgg < 1:514992/246‑1 (MQ=255) ggACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGC‑GTTTCATATCTGCG‑CCGTTGGCAATCAGAATGCCGG‑TGTTGATCTCCTGAATCTG‑ACGCTGCTCGT‑CGGt < 2:401068/249‑1 (MQ=255) ggACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGC‑GTTTCATATCTGCG‑CCGTTGGCAATCAGAATGCCGG‑TGTTGATCTCCTGAATCTG‑ACGCTGCTCGT‑CGGt < 1:579928/249‑1 (MQ=255) gACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCCTCTACTTCGC‑TTTAACGTTGCGGGTGAACGGCGACGATTTTACG‑CCCTTCCTGATACGCCAGCGCAATAATGTAGGTGATGTAGTATTTGCCCTTCGCATTATTGTTGGTCAGCTTCGCCAGCCAGC‑GTTTCCTATCTTCG‑CCGTTGGCAATCCGAATGCCGG‑TGTTTTTCTTCTGAATCTT‑ACGCTGCTCGC‑CTGTgg > 2:522674/1‑250 (MQ=255) tGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCCTCTACTTCGC‑TTAAAAGTTGCGGGTGAACGGCGACGAATTCACG‑CCCCTCCTGCTACGCCAGCGCAATAATGTCGGGGGTGTTGTATTTGCCCCTTGGAGTATTTTTTGTTCGCTTCGCCCGCCACCAGTTTC‑TATTCGCG‑CCGTTTGGCATTAGAACTCCGGTTGTTGTTTCCTTGATTCTG‑CCCCTGC‑CGT‑Cgggtgcca > 2:499088/1‑244 (MQ=255) acacGCTCCAGACGGGAGAGTTGCAGGCGGTTTTTCACGCCCTCTACTTCGC‑TTTAAAGGTGCGGGTGAACGGGGACGATTTTCCG‑CCCCTCCTGATACGCCCGCGCAATAATGTCGGGGGTTTTGTATTTCCCCCGAGGCTTTTTGTTTGTTAACTTCCCCAGCCCCC‑GGTTTCTATTTTCGCCCTTTGGCAACCAAAATG‑CGG‑GGGTGTTTTCCCGTATCTGCCCGCT‑CTCGTCCGGTGGCTTCtttt > 2:524898/1‑249 (MQ=255) cGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGC‑GTTTCATATCTGCG‑CCGTTGGCAATCAGAATGCCGG‑TGTTGATCTCCTGAATCTG‑ACGCTGCTCGT‑CGGTGGCATCTTTGTGCt < 1:348591/250‑1 (MQ=255) gCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGC‑GTTTCATATCTGCG‑CCGTTGGCAATCAGAATGCCGG‑TGTTGATCTCCTGAATCTG‑ACGCTGCTCGT‑CGGTGGCATCttggggagc < 1:316041/250‑8 (MQ=255) gACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGC‑GTTTCATATCTGCG‑CCGTTGGCAATCAGAATGCCGG‑TGTTGATCTCCTGAATCTG‑ACGCTGCTCGT‑CGGTGGCATCTTTGTGCTCAACAAt < 1:648799/250‑1 (MQ=255) agagTGGCAGGCGGTTATTCCCGCCTTCTACTTCGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGC‑GTTTCATATCTGCG‑CCGTTGGCAATCAGAATGCCGG‑TGTTGATCTCCTGAATCTG‑ACGCTGCTCGT‑CGGTGGCATCTTTGTGCTCAACAATGCCGGt < 2:407991/250‑1 (MQ=255) gCGGTTATTCACGCCTTCTACTTCGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGC‑GTTTCATATCTGCG‑CCGTTGGCAATCAGAATGCCGG‑TGTTGATCTCCTGAATCTG‑ACGCTGCTCGT‑CGGTGGCATCTTTGTGCTCCACCATGCCGGTAACTTTGCCg > 2:484057/1‑250 (MQ=255) gCGGTTATTCACGCCTTCTACTTCGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGC‑GTTTCATATCTGCG‑CCGTTGGCAATCAGAATGCCGG‑TGTTGATCTCCTGAATCTG‑ACGCTGCTCGT‑CGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGCCg > 1:94352/1‑250 (MQ=255) ggTTATTCACGCCTTCTACTTCGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGC‑GTTTCATATCTGCG‑CCGTTGGCAATCAGAATGCCGG‑TGTTGATCTCCTGAATCTG‑ACGCTGCTCGT‑CGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGCCGtt > 1:124332/1‑250 (MQ=255) aTTCACGCCTTCTACTTCGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGC‑GTTTCATATCTGCG‑CCGTTGGCAATCAGAATGCCGG‑TGTTGATCTCCTGAATCTG‑ACGCTGCTCGT‑CGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGCCGTTTTCa > 1:550705/1‑250 (MQ=255) cGCCTTCTACTTCGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGC‑GTTTCATATCTGCG‑CCGTTGGCAATCAGAATGCCGG‑TGTTGATCTCCTGAATCTG‑ACGCTGCTCGT‑CGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGCCGTTTTCACGGGt > 1:263770/1‑250 (MQ=255) cTACTTCGC‑TTAAACGGTGCGGATGAACGGAGACGATTTCACG‑CTCTTCCTGATACGCCAGCACAATAATGCAGGTGATTGAGAATTTGCCCTGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGC‑GTTTCATATCTGCG‑ACGTTGGCAATGAGAATGCCGG‑AGTTTATCACCGGAATCTG‑ACGCTTCACGT‑CGGTGGTATTATCATGCGCAACAATGCCGTTAACTTTGCCGTATTCACgagcaataag > 2:639306/1‑241 (MQ=255) tCGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGC‑GTTTCATATCTGCG‑CCGTTGGCAATCAGAATGCCGG‑TGTTGATCTCCTGAATCTG‑ACGCTGCTCGT‑CGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGCCGTTTTCACGGGTGATACGTCCa > 2:411798/1‑249 (MQ=255) cGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGC‑GTTTCATATCTGCG‑CCGTTGGCAATCAGAATGCCGG‑TGTTGATCTCCTGAATCTG‑ACGCTGCTCGT‑CGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGCCGTTTTCACGGGTGATACGTCCAt > 1:413041/1‑249 (MQ=255) aaCGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGC‑GTTTCATATCTGCG‑CCGTTGGCAATCAGAATGCCGG‑TGTTGATCTCCTGAATCTG‑ACGCTGCTCGT‑CGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGCCGTTTTCACGGGTGATACGTCCATAACCgg < 1:85605/249‑1 (MQ=255) gTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGC‑GTTTCATATCTGCG‑CCGTTGGCAATCAGAATGCCGG‑TGTTGATCTCCTGAATCTG‑ACGCTGCTCGT‑CGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGCCGTTTTCACGGGTGATACGTCCATAACCGGTCgg < 1:612929/250‑1 (MQ=255) gTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGC‑GTTTCATATCTGCG‑CCGTTGGCAATCAGAATGCCGG‑TGTTGATCTCCTGAATCTG‑ACGCTGCTCGT‑CGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGCCGTTTTCACGGGTGATACGTCCATAACCGGTCg > 1:472187/1‑249 (MQ=255) gCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTTTTGTTGGTCAGCTTCGCCAGCCAGC‑GTTTCATATCTGCG‑CCGTTGGCAATCAGAATGCCGG‑TGTTGATCTCCTGAATCTG‑ACGCTGCTCGT‑CGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGCCGTTTTCACGGGTGATACGTCCATAACCGGTCGGatc < 2:301081/250‑1 (MQ=255) | TTAATCACGCAACCGGTGCCAATTTTCACGCGATGACCGAGAGTCACGTTGCCCTCGATGATAACGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTATTCACGCCTTCTACTTCGC‑TTAAACGTTGCGGATGAACGGCGACGATTTCACG‑CCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGTATTCGCCCTGAGCATTATTGTTGGTCAGCTTCGCCAGCCAGC‑GTTTCATATCTGCG‑CCGTTGGCAATCAGAATGCCGG‑TGTTGATCTCCTGAATCTG‑ACGCTGCTCGT‑CGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGCCGTTTTCACGGGTGATACGTCCATAACCGGTCGGATC > NZ_CP009273/3907665‑3908158 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |