Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,326,7180TA30.6% 80.7 / 17.0 49Y44F (TAT→TTT) adiCarginine/agmatine antiporter
Reads supporting (aligned to +/- strand):  ref base T (27/7);  new base A (0/15);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 1.08e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATACCAATGGATCTTTCAGAATCGGGAAGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGG‑TTTGATAACCGAGAAACGGGCCAAAGC‑AGCGGCGGGCGTAAGC‑GTAAGAACCACCA‑GGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCC‑ATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTTATTAATA  >  NZ_CP009273/4326476‑4326961
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   
aTACCAATGGATCTTTCAGAATCGGGAAGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGG‑TTTGATAACCGAGAAACGGGCCAAAGC‑AGCGGCGGGCGTAAGC‑GTAAGAACCACCA‑GGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCC‑ATAAATGGc                                                                                                                                                                                                                                              <  1:41472/250‑1 (MQ=255)
                    aTCGGGAAGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGG‑TTTGATAACCGAGAAACGGGCCAAAGC‑AGCGGCGGGCGGAAGC‑GTAAGAACAACCA‑GGGCTTGGGTCGAGGAACGAACTTTTGGCGTATACCATCGCGAGCCCCAGTGCACCGATCATCGTCACCAACCAACC‑CTAAAATGCAATCCCGGCAGGAGAGGc                                                                                                                                                                                                                            >  2:517540/1‑248 (MQ=255)
                     tCGGGAAGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGG‑TTTGATAACCGAGAAACGGGCCAAAGC‑AGCGGCGGGCGTAAGC‑GTAAGAACCACCA‑GGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCC‑ATAAATGGCAATCCCGCCAGTAGAGGCCAg                                                                                                                                                                                                                         >  1:536444/1‑250 (MQ=255)
                     tCGGGAAGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGG‑TTTGATAACCGAGAAACGGGCCAAAGC‑AGCGGCGGGCGTAAGC‑GTAAGAACCACCA‑GGACATGGGCGGAGGAACGACATTTTGGGGTATACCATCGAGAGTCCCAGCGCACCGCTAAATGTCACAGACCCGCC‑ATAAAAGGCCATCCCGGCAGCAGAAGCCAg                                                                                                                                                                                                                         >  2:139071/1‑250 (MQ=255)
                        ggAAGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGG‑TTTGATAACCGAGAAACGGGCCAAAGC‑AGCGGCGGGCGGAAGC‑GTAAGAACCACCA‑GGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGCAAAACGTCACCAAACCTCC‑ATAAATTGCCATCCCCCCCGTAGAAGACAGGtt                                                                                                                                                                                                                      >  2:148865/1‑250 (MQ=255)
                              agTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGG‑TTTGATAACCGAGAAACGGGCCAAAGC‑AGCGGCGGGCGGAAGA‑GGAAGAACCACCA‑GGACTTGGGTCGAGGAACGACATTTTGGCGGATAACATCGAGAACCCCAGGGCACCGATAATCGGCACCAACCATCC‑ATGAATGGCAATCCCGCCAGTAGAGGCCAGGGTTGAAgg                                                                                                                                                                                                                >  2:529856/1‑250 (MQ=255)
                                 aaCTTAAATATCCTCCCCCAATGACCACCATGGCATTATTGCCGACCCAGCAGGCCAGCCAGTAGAGGACGTTGG‑TTTGATAACCGAGAAACGGGCCAAAGC‑AGCGGCGGGCGTAAGC‑GTAAGAACCACCA‑GGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCGGGGCACCGATAATCGCCACCAACCACCC‑ATAAAGGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTaa                                                                                                                                                                                                             <  1:483381/250‑1 (MQ=255)
                                        atatCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGG‑TTTGATAACCGAGAAACGGGCCAAAGC‑AGCGGCGGGCGTAAGC‑GTAAGAACCACCA‑GGACTTGGGGCGAGGAACGACATTTTGGCGGATACCATCCAGAGCCCCAGTGCACCGAAAATCGTCACCAACCATCC‑CTACATGGCAATCCCGCCAGTAGAGGCCAGGGTTGCAGGTAACAGaaa                                                                                                                                                                                                       >  2:211725/1‑249 (MQ=255)
                                                          caccaTGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGG‑TTTGATAACCGAGAAACGGGCCAAAGC‑AGCGGCGGGCGTAAGC‑GTAAGAACCACCA‑GGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCC‑ATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCataata                                                                                                                                                                                    >  1:46121/1‑250 (MQ=255)
                                                                  tgATACTGCCGCCCCAGCGCGCCAGCCAGTAGAGGACGTTGT‑GTATATAACGCAAAAAGGGCCCAAAGC‑GGCGGCGGGGGTAAGC‑GTAACAACCCCAA‑GGACTTGGGTAGAAGAACGCCTTTTTGGCTTATCCCCTCGAGCCCCCCAGTCCCCCGATAATCGCCCCCCCCCACCA‑AAAAATGACAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGa                                                                                                                                                                            <  2:454320/249‑1 (MQ=255)
                                                                                           gccagggggggagTTGGTTTTGAAACCCGA‑AAACCGGCCAAAGCGGGCGGCGGGCTAAGGC‑ATAAGACCCCCCG‑GGACTTGGTGCGAGAAACGACTTTTTGGCGAACACCATCGAGACCCCCAGTGCCCCGATAACCGCCACCAACCACCC‑ATAAATGACAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTaa                                                                                                                                                    <  2:550323/238‑1 (MQ=255)
                                                                                                 ggggACGTTGT‑TTTGAAACCCGAAAAACGGACCAAAGC‑GGCGGGGGGCGTAGGC‑GTAAGAACCACCA‑GGATTTGGGTCGAGGAACGACATTTTGGCTTATACCAGCGAGCGCCCCAGTGCACCGATAATCGTCCCCAACCATCA‑ATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACt                                                                                                                                             <  2:536444/248‑1 (MQ=255)
                                                                                                                 aTAACCGAGAAACGGGCCAAAGC‑AGCGGCGGGCGTAAGC‑GTAAGAACCACCA‑GGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCC‑ATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatcagca                                                                                                                              >  2:611500/1‑250 (MQ=255)
                                                                                                                  aaaCCAAGAACGGGGCAAAAGG‑CGCGGGGGGCGTAGGC‑‑AAAAAACCCCCAGGGACTGGGGTCGAGAAACAAATTTTGGGCGTATACCACCGAGACCCCCAGTGC‑CCCAAAATCCGCCCCAAACAACCAAAAAATGCCACCCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatcagcat                                                                                                                             <  2:49852/249‑1 (MQ=255)
                                                                                                                   aaCCGAGACGCGGCACAGCGC‑GGCGGCGGGCG‑AAGCGGTAAAACCCCCAA‑GTACTTGGCGCGAGGAACGACTTTTTGCCATATACCATCGAGGCCCCCAGCACACCGATAATCCTCACCACCCACCA‑AAAAATGGCACTCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatcagcatc                                                                                                                            <  2:617641/250‑1 (MQ=255)
                                                                                                                   aaCCGAGAAACGGGCCAAAGC‑AGTGGCGGGCGTAAGC‑GTAAGAACCACCA‑GGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCC‑ATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGGAACAGAAAAACACCTGACCCCATAATATTCCCCGACAACATCAGGGTGACGGGGATTAAGACCACTTTGTGagcatcagcatc                                                                                                                            >  1:417639/1‑250 (MQ=255)
                                                                                                                            aCGGGCCAAAGC‑AGCGGCGGGCGTAAGC‑GTAAGAACCACCA‑GGACTTGGGTCGCGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCC‑ATAAATTGCAATCCCGCCAGGAGAGGCCAGGTTTGCAGGTAACAGAAAAACAACTGACCCCATCATATTCCCCGACCCCATCCGGGTGACGGGGATTAAGCCCACTTTCTGAGCTTCAACATTCGAAGCCa                                                                                                                    >  2:502621/1‑249 (MQ=255)
                                                                                                                                  cAAAGC‑AGCGGGGGGCGTAAGG‑ATAAGACCCACGG‑GGATTGGGGTGGAGCACCGACATTTGGGTGAATCCCACAGAGAGCCCCGTGGCCCCAAAAACCGCCACCACCCATCC‑AAAAAGGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTaa                                                                                                             <  2:306223/250‑1 (MQ=255)
                                                                                                                                  aaaaGC‑GGCGGCGGGCGTAAGC‑GAAAGAACCACCA‑GGCCGTGGGTCGAGAAACGACTTTTTGGCGTATACCAGCGAGACCCCCAGTGCCCCGAAAATCGCCACCAACAATCA‑ATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTaa                                                                                                             <  2:592260/249‑1 (MQ=255)
                                                                                                                                     agc‑agcGGCGGGCGTAAGC‑GTAAGAACCACCA‑GGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCC‑ATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACt                                                                                                          >  2:272138/1‑250 (MQ=255)
                                                                                                                                         agcGGGGGGCGTAAGC‑GTAAGAACCACCA‑GGCCTTGGGTCGAGGAACGACATTTTGGCTTATCCCACCGAGACCCCCAGTGCCCCGATAATCGTCCCCAACCATCC‑AAAAATGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCt                                                                                                       <  1:205666/250‑1 (MQ=255)
                                                                                                                                                ggcgAAAGC‑GTAAGAACCACCA‑GGCCTGGGGTCGAGAAACGACATTTGGGCGTATCCCATCGAGCGCCCCAGTGCACCGATAATCGTCCCCAACCATCC‑AAAAAGGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAgg                                                                                                <  1:106204/250‑1 (MQ=255)
                                                                                                                                                 gcgtaagc‑gaaaAACCCCCCA‑GGACTGGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGACCCCCAGTCCACCGATAATCGTCACCACCCATCC‑ATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGc                                                                                               <  1:236994/250‑1 (MQ=255)
                                                                                                                                                          gtaagAACCACCA‑GGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCC‑ATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCg                                                                                       >  1:411303/1‑250 (MQ=255)
                                                                                                                                                                   acca‑GGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCC‑ATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATa                                                                               >  2:248131/1‑249 (MQ=255)
                                                                                                                                                                    cca‑GGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCC‑ATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATaaa                                                                             >  1:257909/1‑250 (MQ=255)
                                                                                                                                                                      a‑GGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCC‑ATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGACAAACACATGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAACCTCCTGCGCAGGAGTGCATCGTGaacatacat                                                                            >  2:540658/1‑241 (MQ=255)
                                                                                                                                                                       agtggtggggaagAGGAACGACTTTGTGGCGAAGACCACCGAGACCCCCAGTGCACCGAAAATCGTCACCACCCAACA‑AAAAAGGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAAtt                                                                           <  2:584306/238‑1 (MQ=255)
                                                                                                                                                                         gacgggggTCGGGGACCGACTTTTGGGCGTATCCCATCGAGAGCCCCAGTGCCCCGATAATCGTCACCAACCACCA‑AAAAATGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTAc                                                                         <  1:84857/245‑1 (MQ=255)
                                                                                                                                                                          aCTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCC‑ATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACg                                                                        >  1:593530/1‑250 (MQ=255)
                                                                                                                                                                             tGGGGCGAGGAACATCATTGGGGCGTATACCATCGAGAGCCCCAGTGCCCCGATTTTCGACACCGACCCCCA‑AAAAAGGGCAAGCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTATGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtc                                                                      <  1:434795/249‑1 (MQ=255)
                                                                                                                                                                               ggTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACAGATAATCGTCACCAACCATCC‑ATAAATGGCAATCCCGCCAGAAGAGGCCAGGTTTGCAGGCAACAGAAAAACACCTGACGCCATAATATTCTCCGAGACCATCAGGGTGACGGGGATTAAGTCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAACCTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcata                                                                   >  2:133405/1‑250 (MQ=255)
                                                                                                                                                                                gTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCC‑ATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcatat                                                                  >  2:499375/1‑250 (MQ=255)
                                                                                                                                                                                  ggagaacagACATTTTGGCATAGACCATAGAAACCCAGAGTGCCACGATAATCGTCACAACCCAACA‑AAAAAAAAATACACCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcatatca                                                                <  2:303601/242‑1 (MQ=255)
                                                                                                                                                                                  cGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCC‑ATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAACACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCTCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcatatca                                                                >  1:377191/1‑250 (MQ=255)
                                                                                                                                                                                   gAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCC‑ATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcatatca                                                                >  1:616257/1‑249 (MQ=255)
                                                                                                                                                                                     ggAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCC‑ATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATAc                                                             >  1:130797/1‑250 (MQ=255)
                                                                                                                                                                                       aaCGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCC‑ATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGc                                                           >  1:427382/1‑250 (MQ=255)
                                                                                                                                                                                                  ggCGTATACCATCGAGAGCCCCAATGCCCCGATAATCGTCACCAACCATCC‑ATAAACGGCAATCCCGCCAGTAGAGCCCAGGCTTTTACGCAACAGAAAAACACCTGACCCCATAATATTCCCCGACAGCATCAGGGTGACGGGCATTAAGCCCACTTTGTGTGCTTCAGCATCCGAAGACACAATTAAACTCCTGCGTAGCCGACCTTCGTGCCTATAAATCACGACATACCACACACGTGCCTTCGAt                                                >  2:397242/1‑250 (MQ=255)
                                                                                                                                                                                                          ccaccaagacCCCCAGTGCACCGAAAACCGTCCCCAACCACCC‑AAAAAGGGAAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAat                                        <  1:344918/244‑1 (MQ=255)
                                                                                                                                                                                                            accGAGAGCCCCAGTGCACCAAAAATCGTCACAAACCACCC‑AAAAATGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAatta                                      <  1:311582/248‑1 (MQ=255)
                                                                                                                                                                                                            aTCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCC‑ATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAatta                                      >  1:503733/1‑250 (MQ=255)
                                                                                                                                                                                                              cGAGACCCCCAGTGCCCCGATAACCGTCCCCAACCACCA‑AAAAATGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAattatt                                    <  1:502544/250‑1 (MQ=255)
                                                                                                                                                                                                                      ccAGTGCACCAATAATCGTCACCAACCATCC‑ATAAATGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCg                            <  1:574636/250‑1 (MQ=255)
                                                                                                                                                                                                                                gATAATCGTCACCAACCATCC‑ATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGCATTATTATCGACCGGGTTATCGCt                  >  1:388434/1‑250 (MQ=255)
                                                                                                                                                                                                                                gATAATCGTCACCAACCATCC‑ATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCt                  >  2:300689/1‑250 (MQ=255)
                                                                                                                                                                                                                                   aaTCGTCACCACCCACCC‑AAAAAGGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGAt               <  1:442225/250‑1 (MQ=255)
                                                                                                                                                                                                                                         cACCAACCACCA‑AAAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGtt         <  1:395103/250‑1 (MQ=255)
                                                                                                                                                                                                                                              cccaccc‑atAAATGGCACCGCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTTATTaa    <  1:607124/249‑1 (MQ=255)
                                                                                                                                                                                                                                              accaTCC‑ATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTTATTaa    >  2:282978/1‑250 (MQ=255)
                                                                                                                                                                                                                                                catcc‑atAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTTATTAata  >  2:118278/1‑250 (MQ=255)
                                                                                                                                                                                                                                                 atcc‑atAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTTATTAata  >  1:54109/1‑249 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   
ATACCAATGGATCTTTCAGAATCGGGAAGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGG‑TTTGATAACCGAGAAACGGGCCAAAGC‑AGCGGCGGGCGTAAGC‑GTAAGAACCACCA‑GGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCC‑ATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTTATTAATA  >  NZ_CP009273/4326476‑4326961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑