| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,884,307 | A→T | D161E (GAT→GAA) | yeaY ← | Slp family lipoprotein YeaY |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,884,307 | 0 | A | T | 97.1% | 113.2 / NA | 35 | D161E (GAT→GAA) | yeaY | Slp family lipoprotein YeaY |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (32/2); minor base C (1/0); total (33/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCT > NZ_CP009273/1884061‑1884543 | cGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCAGTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGTTc > 1:393384/1‑249 (MQ=255) gTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAAGGACGTCCACTACCGCCACAAAACCAAGGTTCaa > 2:600004/1‑250 (MQ=255) tAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCAATAAAACCATGGCTCAATc > 2:309001/1‑250 (MQ=255) taataTTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGTTCAATCGGCt > 1:352567/1‑249 (MQ=255) atatTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGTTCAATCGGCTGAg > 1:50965/1‑250 (MQ=255) atatTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGTTCAATCGGCTGAg > 2:305455/1‑250 (MQ=255) ttAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGTTCAATCGGCTGAggcg > 1:451753/1‑250 (MQ=255) cTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGTTCAATCGGCTGAGGCGGCa > 1:571799/1‑249 (MQ=255) cTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGTTCAATCGGCTGAGGCGGCa > 1:300248/1‑249 (MQ=255) cgCTTGATAATTACCCAACGAAAAGGTTGCGATGCGCGTCACTATTTATTTTTATCTTTACCGTAAGATTGCAGAAACAGCGGACCCGCCGCTGTTTCTTTTTCTTTAAACACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATTCCAGCCCCATCCGCCTTAGTCATTGGGCCAGCCACGACCGCCATAAATCCATCGTTCAATCTGCTGAGGCAGCATAATCACctgtt > 2:489640/2‑248 (MQ=255) tGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGTTCAATCGGCTGAGGCGGCATAATCACCTGCTGGGt > 1:569376/1‑250 (MQ=255) gAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGTTCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGttt > 1:338935/1‑250 (MQ=255) aaaaGGTTGCGAAGCGCATCACTATTTATTTTTATCTTTAACGTAAGAATGCAGAACCAGCGCACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGCTACAACTGTTTGTACTCTCGCGCGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCAGGACCGCCATAAAACCATGGTTCAATCTGCTGCGGCAGCCTAATACCCTGCTGGCGTAAACGCCAACgctgc > 2:512938/1‑246 (MQ=255) aaGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGTTCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTAc > 2:367996/1‑250 (MQ=255) cgcgTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTCTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGTTCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTg > 1:234939/1‑250 (MQ=255) cACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGTTCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAATCAGTTACTTGCATCa > 1:356038/1‑250 (MQ=255) aCTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGTTCAATCGGCTGAGGCGCCATAATCACCTGCTGGGTTAAATGCCCACGTTCGCAACCCCTTACTCGCCTCAc > 2:421401/1‑250 (MQ=255) tatttattTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGTTCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCa > 2:466546/1‑250 (MQ=255) aCCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGTTCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTa > 2:558944/1‑250 (MQ=255) gTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGTTCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTAttt > 2:59107/1‑249 (MQ=255) gTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGTTCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCCACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCCCACCACTGAttt > 2:389802/1‑249 (MQ=255) tttctttttcttTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGTTCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTgg > 2:506741/1‑250 (MQ=255) ctttttctttAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGTTCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGccc > 2:104187/1‑250 (MQ=255) ttACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGTTCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCCTAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCCCACCAGTGATTGGCCCGACTACCGTAACCCGTTGTACACGGAAATc > 2:4703/1‑250 (MQ=255) cAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGTTCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCAc > 1:76919/1‑250 (MQ=255) cgcgGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGTTCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCg > 2:637192/1‑250 (MQ=255) gggCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGTTCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTca > 2:569577/1‑250 (MQ=255) gggATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGTTCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCg > 2:198089/1‑250 (MQ=255) aTTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGTTCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCa > 1:635409/1‑250 (MQ=255) aTCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGTTCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACcgc > 1:126548/1‑249 (MQ=255) aGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGTTCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTtc > 1:586884/1‑250 (MQ=255) cgacccaCGCCCGCCATAAAACCATGGTTCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTc < 2:305675/246‑1 (MQ=255) ccagccaCGACCGCCATAAAACCATGGTTCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAACGTTCTCCCCGCGTc > 2:325539/1‑250 (MQ=255) cGCCATAAAACCATGGTTCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTcc > 1:491239/1‑250 (MQ=255) cATAAAACCATGGTTCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCt < 1:529216/250‑1 (MQ=255) | CGTTAACATAATATTAACTCATCATACCAGCTTGATAATTACCCAACGAAAAGGTTGCGAAGCGCGTCACTATTTATTTTTATCTTTACCGTAAGAATGCAGAAACAGCGGACCAGCCGCTGTTTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCT > NZ_CP009273/1884061‑1884543 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |