Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,446,404	A→G	V499A (GTT→GCT)	paaZ ←	phenylacetic acid degradation bifunctional protein PaaZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,446,404	0	A	G	100.0%	146.9 / NA	44	V499A (GTT→GCT)	paaZ	phenylacetic acid degradation bifunctional protein PaaZ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/22);  total (22/22)

CACCCGCTCACCGAAAATAGATTCGGCAGCAGCAATCTTATCCATATGTGCATAGAAATGATCGCCGCTGAGGCAAGCAAAGTTAACAATATCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAATTCGCCCATGCGTACGTGCCGCGTCGGCAATAAACTGACGCGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGC  >  NZ_CP009273/1446157‑1446635                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                         caccCGCTCACCGAAAATAGATTCGGCAGCAGCAATCTTATCCATATGTGCATAGAAATGATCGCCGCTGAGGCAAGCAAAGTTAACAATATCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCa                                                                                                                                                                                                                                        <  1:191857/250‑1 (MQ=255)                aaTAGATTCGGCAGCAGCAATCTTATCCATATGTGCATAGAAATGATCGCCGCTGAGGCAAGCAAAGTTAACAATATCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATg                                                                                                                                                                                                                         <  2:533445/250‑1 (MQ=255)                  tAGATTCGGCAGCAGCAATCTTATCCATATGTGCATAGAAATGATCGCCGCTGAGGCAAGCAAAGTTAACAATATCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTTGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCAACGTCGGCCTACCCTGAGCAGCGGTTCGCTGCACGt                                                                                                                                                                                                                        >  2:334619/1‑249 (MQ=255)                               agcaATCTTATCCATATGTGCATAGAAATGATCGCCGCTGAGGCAAGCAAAGTTAACAATATCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTCGCTCCCCAAAATATTTTCGGGACGGATGAAAACGGTCTTCTTCGCCTTTTGCACCGCGCACCCCCTGTTTTCTGCAAGCGGCCAGCCTCGTCGGACCTCCCTGAGCAGCGGTTCGCTGACTGTAAACTTTCTCCGCt                                                                                                                                                                                                          >  2:523153/1‑250 (MQ=255)                                gcaATCTTATCCATATGTGCATAGAAATGATCGCCGCTGAGGCAAGCAAAGTTAACAATATCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGTGGAAAGGATGAATACGATCTTTTTCGACTTTCGCAACGCGCACCCCCTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGGTCGCCGCCTGTACTGTTTTACCGCTc                                                                                                                                                                                                         >  2:475383/1‑250 (MQ=255)                                        aTCCATATGTGCATAGAAATGATCGCCGCTGAGGCAAGCAAAGTTAACAATATCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCg                                                                                                                                                                                                 >  1:48871/1‑250 (MQ=255)                                            atatGTGCATAGAAATGAGCGCCGCTGAGGCAAGCAAAGTTAACAATATCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTa                                                                                                                                                                                             <  2:171340/250‑1 (MQ=255)                                                          aTGATCGCCGCTGAGGCAAGCAAAGTTAACAATATCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGGCTACCCTGAGCAGCGGTTCGCTGCATGTAAAGTTTTACCGCCCGTAAACCGCCTAC‑TTCTTCACCGccc                                                                                                                                                                              >  2:275160/1‑249 (MQ=255)                                                              tCGCCGCTGAGGCAAGCAAAGTTAACAATATCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAAAGGATGAATACGAACTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGGAAACCGCCTAA‑TTCTTCACCGCCTCCTg                                                                                                                                                                          >  1:224301/1‑250 (MQ=255)                                                                   gcTGAGGCAAGCAAAGTTAACAATATCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCCAGCATCGTCGGCCTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGa                                                                                                                                                                     >  1:70965/1‑250 (MQ=255)                                                                       aGGCAAGCAAAGTTAACAATATCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAg                                                                                                                                                                 >  1:271885/1‑250 (MQ=255)                                                                              caaAGTTAACAATATCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCCAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCCTCACCGCCCCCCGCCCGACCCGGCccacc                                                                                                                                                          >  2:499957/1‑250 (MQ=255)                                                                                  gTTAACAATATCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATg                                                                                                                                                       >  2:292046/1‑249 (MQ=255)                                                                                   ttAACAATATCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTa                                                                                                                                                     <  1:126197/250‑1 (MQ=255)                                                                                       cAATATCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATAAGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACCACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCCCCCGTAAAACGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAAGCCCACCATGTAcccg                                                                                                                                                 >  1:61052/1‑248 (MQ=255)                                                                                            tCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTtgt                                                                                                                                             <  1:499957/249‑1 (MQ=255)                                                                                               gCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTgg                                                                                                                                           >  1:553236/1‑248 (MQ=255)                                                                                                      tCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGGGGAACGGATGAATACGATCTTATTCGACTTTCGCACCGCGCAACCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCCGAGCAGCGGTTCGACGCATGTAATGTTTCACCGCTCGTAAACCGCCTAATTTTTTCTACG‑ATCCTGCGGGACCCGGCCCACCATGTGACAGTTGTGGCAggggggg                                                                                                                                  >  2:454731/1‑249 (MQ=255)                                                                                                      tCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTggg                                                                                                                                    <  2:294250/248‑1 (MQ=255)                                                                                                           gtgCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCa                                                                                                                              <  1:204747/249‑1 (MQ=255)                                                                                                               ggcggGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGccc                                                                                                                         >  2:145689/1‑250 (MQ=255)                                                                                                                 cggGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCgg                                                                                                                       >  2:90897/1‑250 (MQ=255)                                                                                                                        tCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTc                                                                                                                <  1:174748/250‑1 (MQ=255)                                                                                                                               gCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGcc                                                                                                         <  2:221696/250‑1 (MQ=255)                                                                                                                                   tCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGAc                                                                                                      <  1:227639/249‑1 (MQ=255)                                                                                                                                        tGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCATCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCa                                                                                                >  1:71706/1‑250 (MQ=255)                                                                                                                                            tGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCAtt                                                                                              >  2:57789/1‑248 (MQ=255)                                                                                                                                                       aaaaTATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAAt                                                                                  <  1:131296/249‑1 (MQ=255)                                                                                                                                                        aaaTATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAATTc                                                                                <  1:449164/250‑1 (MQ=255)                                                                                                                                                           tatTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAATTCGcc                                                                             >  2:14300/1‑250 (MQ=255)                                                                                                                                                                 tggAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAATTCGCCCATGc                                                                        <  1:475383/248‑1 (MQ=255)                                                                                                                                                                            aaTCCGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAATTCGCCCATGCGTACGTGCcgc                                                             <  1:400646/249‑1 (MQ=255)                                                                                                                                                                            aaTACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAATTCGCCCATGCGTACGTGCcgc                                                             <  1:410594/249‑1 (MQ=255)                                                                                                                                                                              tACGATCTTCTTCGACTTTCGCACCGCGCACCCCCTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAATTCGCCCATGCGTACGTGCCGCGTc                                                          <  1:482132/250‑1 (MQ=255)                                                                                                                                                                               aCGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTACCGACTCTTCATTGAGGATCTGAATTCGCCCATGCGTACGTGCCGCGTCg                                                         >  1:433287/1‑250 (MQ=255)                                                                                                                                                                                    cttcttCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAATTCGCCCATGCGTACGTGCCGCGTCGGCaa                                                     >  2:158955/1‑249 (MQ=255)                                                                                                                                                                                       cttcGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAATTCGCCCATGCGTACGTGCCGCGTCGGCAATaa                                                  <  1:292046/249‑1 (MQ=255)                                                                                                                                                                                                tCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAATTCGCCCATGCGTACGTGCCGCGTCGGCAATAAACTGAcgcg                                         <  1:81350/249‑1 (MQ=255)                                                                                                                                                                                                        cgcACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAATTCGCCCATGCGTACGTGCCGCGTCGGCAATAAACTGACGCGCAATTTGCg                                <  1:463217/250‑1 (MQ=255)                                                                                                                                                                                                        cgcACCCACTGTTTACTGATAGCGGCAAGCAACGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAATTCGCCCATGCGTACGTGCCGCGTCGGCAATAAACTGACGCGCAATTTGCg                                <  2:271813/250‑1 (MQ=255)                                                                                                                                                                                                                                   aGCATCGTCGGACTCCCCTGAGCGGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAATTCGCCCATGCGTACGTGCCGCGTCGGCAATAAACTGACGCGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCaa     <  2:12633/250‑1 (MQ=255)                                                                                                                                                                                                                                   aGCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAATTCGCCCATGCGTACGTGCCGCGTCGGCAATAAACTGACGCGCAATTTGCGGATCAGCCGTCACCAGCGGTCCCGc       >  1:570710/1‑248 (MQ=255)                                                                                                                                                                                                                                    gCATCGTCGGACTACCCTGAGCAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAATTCGCCCATGCGTACGTGCCGCGTCGGCAATAAACTGACGCGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAg    >  2:216774/1‑250 (MQ=255)                                                                                                                                                                                                                                      aTCGTCGGCCGACCCTGAGCGGCGGTTCGCTGCATGTAAGGTTTCACCGCTCGTAACCAGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAATTCGCCCATGCGTACGTGCCGCGTCGGCAATAAACTGACGCGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGc  <  2:514432/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                         CACCCGCTCACCGAAAATAGATTCGGCAGCAGCAATCTTATCCATATGTGCATAGAAATGATCGCCGCTGAGGCAAGCAAAGTTAACAATATCGGCCTCTGTCATTGTGCGGCGGGGAGTCAACAGGCTGTCGCCTGGTTGTAGCTCCTCAAAATATTTGCGGAACGGATGAATACGATCTTCTTCGACTTTCGCACCGCGCACCCACTGTTTACTGATAGCGGCAAGCATCGTCGGACTACCCTGAACAGCGGTTCGCTGCATGTAATGTTTCACCGCTCGTAAACCGCCTAA‑TTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAATTCGCCCATGCGTACGTGCCGCGTCGGCAATAAACTGACGCGCAATTTGCGGATCAGCCGTCACCAGCGTTCCCGCAAGGC  >  NZ_CP009273/1446157‑1446635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑